Molecular cytogenetic study on the ploidy status in Acipenser mikadoi
Identifieur interne : 000715 ( Istex/Corpus ); précédent : 000714; suivant : 000716Molecular cytogenetic study on the ploidy status in Acipenser mikadoi
Auteurs : H. Zhou ; T. Fujimoto ; S. Adachi ; S. Abe ; E. Yamaha ; K. AraiSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2013-02.
Abstract
The ploidy status of Acipenser mikadoi was examined using nuclear DNA contents, karyotypes and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. In flow‐cytometrically sorted specimens with 8.2–9.1 pg DNA content per somatic cell, i.e. genetic diploid, the best informative metaphase with 268 chromosomes had 80 biarmed meta‐ or submetacentric (M or SM) chromosomes, 48 monoarmed telocentric (T) chromosomes and 140 microchromosomes. In genetic triploid specimens with 12.6–13.0 pg DNA content, the best informative metaphase with 402 chromosomes showed 120 biarmed M or SM, 72 monoarmed T chromosomes and 210 microchromosomes. The rDNA FISH detected a maximum 18 and 27 signals in the diploid and triploid A. miakdoi, respectively. The obtained findings thus corroborated a clear parallel between nuclear DNA contents and karyological or FISH profiles in the genetic diploid and triploid specimens, suggesting 1.5 times chromosome complements of diploid counterparts or three sets of homologues in the triploid sturgeons. Moreover, the estimated genome size and the observed molecular cytogenetic features in the diploid A. mikadoi strongly suggest that this species is a member of a functional tetraploid group recently proposed in the literature.
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DOI: 10.1111/jai.12109
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Zhou</name><affiliation><mods:affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>He Zhou, Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University, 3‐1‐1 Minato‐cho, Hakodate, Hokkaido 041‐8611, Japan.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: xiaoben0603@yahoo.co.jp</mods:affiliation></affiliation></author><author><name sortKey="Fujimoto, T" sort="Fujimoto, T" uniqKey="Fujimoto T" first="T." last="Fujimoto">T. Fujimoto</name><affiliation><mods:affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</mods:affiliation></affiliation></author><author><name sortKey="Adachi, S" sort="Adachi, S" uniqKey="Adachi S" first="S." last="Adachi">S. Adachi</name><affiliation><mods:affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</mods:affiliation></affiliation></author><author><name sortKey="Abe, S" sort="Abe, S" uniqKey="Abe S" first="S." last="Abe">S. Abe</name><affiliation><mods:affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</mods:affiliation></affiliation></author><author><name sortKey="Yamaha, E" sort="Yamaha, E" uniqKey="Yamaha E" first="E." last="Yamaha">E. Yamaha</name><affiliation><mods:affiliation>Nanae Fresh‐Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Nanae, Kameda, Hokkaido, Japan</mods:affiliation></affiliation></author><author><name sortKey="Arai, K" sort="Arai, K" uniqKey="Arai K" first="K." last="Arai">K. Arai</name><affiliation><mods:affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-02">2013-02</date><biblScope unit="volume">29</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="51">51</biblScope><biblScope unit="page" to="55">55</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">1A04E51BC83828FAA431217CB4D61FA9F39F7745</idno><idno type="DOI">10.1111/jai.12109</idno><idno type="ArticleID">JAI12109</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The ploidy status of Acipenser mikadoi was examined using nuclear DNA contents, karyotypes and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. In flow‐cytometrically sorted specimens with 8.2–9.1 pg DNA content per somatic cell, i.e. genetic diploid, the best informative metaphase with 268 chromosomes had 80 biarmed meta‐ or submetacentric (M or SM) chromosomes, 48 monoarmed telocentric (T) chromosomes and 140 microchromosomes. In genetic triploid specimens with 12.6–13.0 pg DNA content, the best informative metaphase with 402 chromosomes showed 120 biarmed M or SM, 72 monoarmed T chromosomes and 210 microchromosomes. The rDNA FISH detected a maximum 18 and 27 signals in the diploid and triploid A. miakdoi, respectively. The obtained findings thus corroborated a clear parallel between nuclear DNA contents and karyological or FISH profiles in the genetic diploid and triploid specimens, suggesting 1.5 times chromosome complements of diploid counterparts or three sets of homologues in the triploid sturgeons. Moreover, the estimated genome size and the observed molecular cytogenetic features in the diploid A. mikadoi strongly suggest that this species is a member of a functional tetraploid group recently proposed in the literature.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>H. Zhou</name><affiliations><json:string>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</json:string><json:string>He Zhou, Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University, 3‐1‐1 Minato‐cho, Hakodate, Hokkaido 041‐8611, Japan.E‐mail:</json:string><json:string>E-mail: xiaoben0603@yahoo.co.jp</json:string></affiliations></json:item><json:item><name>T. Fujimoto</name><affiliations><json:string>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</json:string></affiliations></json:item><json:item><name>S. Adachi</name><affiliations><json:string>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</json:string></affiliations></json:item><json:item><name>S. Abe</name><affiliations><json:string>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</json:string></affiliations></json:item><json:item><name>E. Yamaha</name><affiliations><json:string>Nanae Fresh‐Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Nanae, Kameda, Hokkaido, Japan</json:string></affiliations></json:item><json:item><name>K. 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In genetic triploid specimens with 12.6–13.0 pg DNA content, the best informative metaphase with 402 chromosomes showed 120 biarmed M or SM, 72 monoarmed T chromosomes and 210 microchromosomes. The rDNA FISH detected a maximum 18 and 27 signals in the diploid and triploid A. miakdoi, respectively. The obtained findings thus corroborated a clear parallel between nuclear DNA contents and karyological or FISH profiles in the genetic diploid and triploid specimens, suggesting 1.5 times chromosome complements of diploid counterparts or three sets of homologues in the triploid sturgeons. 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No. K‐2;</note><note>Ministry of Education, Culture, Sports and Technology (MEXT)</note><note>Exploratory Research - No. 18658073;</note><note>Challenging Exploratory Research - No. 21658067;</note><note>Japan Society for the Promotion of Science (JSPS)</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Molecular cytogenetic study on the ploidy status in Acipenser mikadoi</title><author xml:id="author-1"><persName><forename type="first">H.</forename><surname>Zhou</surname></persName><email>xiaoben0603@yahoo.co.jp</email><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation><affiliation>He Zhou, Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University, 3‐1‐1 Minato‐cho, Hakodate, Hokkaido 041‐8611, Japan.E‐mail:</affiliation></author><author xml:id="author-2"><persName><forename type="first">T.</forename><surname>Fujimoto</surname></persName><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation></author><author xml:id="author-3"><persName><forename type="first">S.</forename><surname>Adachi</surname></persName><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation></author><author xml:id="author-4"><persName><forename type="first">S.</forename><surname>Abe</surname></persName><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation></author><author xml:id="author-5"><persName><forename type="first">E.</forename><surname>Yamaha</surname></persName><affiliation>Nanae Fresh‐Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Nanae, Kameda, Hokkaido, Japan</affiliation></author><author xml:id="author-6"><persName><forename type="first">K.</forename><surname>Arai</surname></persName><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-02"></date><biblScope unit="volume">29</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="51">51</biblScope><biblScope unit="page" to="55">55</biblScope></imprint></monogr><idno type="istex">1A04E51BC83828FAA431217CB4D61FA9F39F7745</idno><idno type="DOI">10.1111/jai.12109</idno><idno type="ArticleID">JAI12109</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-10-19</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>The ploidy status of Acipenser mikadoi was examined using nuclear DNA contents, karyotypes and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. In flow‐cytometrically sorted specimens with 8.2–9.1 pg DNA content per somatic cell, i.e. genetic diploid, the best informative metaphase with 268 chromosomes had 80 biarmed meta‐ or submetacentric (M or SM) chromosomes, 48 monoarmed telocentric (T) chromosomes and 140 microchromosomes. In genetic triploid specimens with 12.6–13.0 pg DNA content, the best informative metaphase with 402 chromosomes showed 120 biarmed M or SM, 72 monoarmed T chromosomes and 210 microchromosomes. The rDNA FISH detected a maximum 18 and 27 signals in the diploid and triploid A. miakdoi, respectively. The obtained findings thus corroborated a clear parallel between nuclear DNA contents and karyological or FISH profiles in the genetic diploid and triploid specimens, suggesting 1.5 times chromosome complements of diploid counterparts or three sets of homologues in the triploid sturgeons. Moreover, the estimated genome size and the observed molecular cytogenetic features in the diploid A. mikadoi strongly suggest that this species is a member of a functional tetraploid group recently proposed in the literature.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Original Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-12-08">Received</change><change when="2012-04-28">Registration</change><change when="2012-10-19">Created</change><change when="2013-02">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/1A04E51BC83828FAA431217CB4D61FA9F39F7745/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:id="jai12109" xml:lang="en"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id></idGroup><titleGroup><title sort="JOURNAL OF APPLIED ICHTHYOLOGY" type="main">Journal of Applied Ichthyology</title><title type="short">J. 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Ichthyol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="02101"><doi origin="wiley">10.1111/jai.2012.29.issue-1</doi><copyright ownership="publisher">Journal compilation © 2013 Journal of Applied Ichthyology</copyright><numberingGroup><numbering type="journalVolume" number="29">29</numbering><numbering type="journalIssue">1</numbering></numberingGroup><coverDate startDate="2013-02">February 2013</coverDate></publicationMeta><publicationMeta level="unit" position="9" status="forIssue" type="article"><doi>10.1111/jai.12109</doi><idGroup><id type="unit" value="JAI12109"></id></idGroup><countGroup><count number="5" type="pageTotal"></count></countGroup><titleGroup><title type="articleCategory">Original Article</title><title type="tocHeading1">Sturgeon Articles</title></titleGroup><copyright ownership="publisher">© 2012 Blackwell Verlag GmbH</copyright><eventGroup><event date="2011-12-08" type="manuscriptReceived"></event><event date="2012-04-28" type="manuscriptAccepted"></event><event agent="SPS" date="2012-10-19" type="xmlCreated"></event><event type="publishedOnlineEarlyUnpaginated" date="2012-12-22"></event><event type="firstOnline" date="2012-12-22"></event><event type="publishedOnlineFinalForm" date="2013-01-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.6.4 mode:FullText" date="2015-10-02"></event></eventGroup><numberingGroup><numbering type="pageFirst">51</numbering><numbering type="pageLast">55</numbering></numberingGroup><correspondenceTo><lineatedText><line><b>Author's address:</b> He Zhou, Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University, 3‐1‐1 Minato‐cho, Hakodate, Hokkaido 041‐8611, Japan.</line><line>E‐mail: <email>xiaoben0603@yahoo.co.jp</email></line></lineatedText></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI12109.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Molecular cytogenetic study on the ploidy status in <i><fc>A</fc>cipenser mikadoi</i></title><title type="shortAuthors">H. Zhou et al.</title></titleGroup><creators><creator affiliationRef="#jai12109-aff-0001" corresponding="yes" creatorRole="author" xml:id="jai12109-cr-0001"><personName><givenNames>H.</givenNames> <familyName>Zhou</familyName></personName></creator><creator affiliationRef="#jai12109-aff-0001" creatorRole="author" xml:id="jai12109-cr-0002"><personName><givenNames>T.</givenNames> <familyName>Fujimoto</familyName></personName></creator><creator affiliationRef="#jai12109-aff-0001" creatorRole="author" xml:id="jai12109-cr-0003"><personName><givenNames>S.</givenNames> <familyName>Adachi</familyName></personName></creator><creator affiliationRef="#jai12109-aff-0001" creatorRole="author" xml:id="jai12109-cr-0004"><personName><givenNames>S.</givenNames> <familyName>Abe</familyName></personName></creator><creator affiliationRef="#jai12109-aff-0002" creatorRole="author" xml:id="jai12109-cr-0005"><personName><givenNames>E.</givenNames> <familyName>Yamaha</familyName></personName></creator><creator affiliationRef="#jai12109-aff-0001" creatorRole="author" xml:id="jai12109-cr-0006"><personName><givenNames>K.</givenNames> <familyName>Arai</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="JP" type="organization" xml:id="jai12109-aff-0001"><orgDiv>Graduate School of Fisheries Sciences</orgDiv> <orgName>Hokkaido University</orgName> <address><city>Hakodate, Hokkaido</city> <country>Japan</country></address></affiliation><affiliation countryCode="JP" type="organization" xml:id="jai12109-aff-0002"><orgDiv>Nanae Fresh‐Water Laboratory</orgDiv> <orgDiv>Field Science Center for Northern Biosphere</orgDiv> <orgName>Hokkaido University</orgName> <address><city>Nanae, Kameda, Hokkaido</city> <country>Japan</country></address></affiliation></affiliationGroup><fundingInfo><fundingAgency>Grants‐in‐Aid </fundingAgency><fundingNumber>K‐2</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Ministry of Education, Culture, Sports and Technology (MEXT)</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Exploratory Research</fundingAgency><fundingNumber>18658073</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Challenging Exploratory Research</fundingAgency><fundingNumber>21658067</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Japan Society for the Promotion of Science (JSPS)</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:id="jai12109-abs-0001"><title type="main">Summary</title><p>The ploidy status of <i>Acipenser mikadoi</i> was examined using nuclear DNA contents, karyotypes and fluorescence <i>in situ</i> hybridization (<fc>FISH</fc>) with 5.8S + 28S <fc>rDNA</fc> as a probe. In flow‐cytometrically sorted specimens with 8.2–9.1 pg <fc>DNA</fc> content per somatic cell, i.e. genetic diploid, the best informative metaphase with 268 chromosomes had 80 biarmed meta‐ or submetacentric (<fc>M</fc> or <fc>SM</fc>) chromosomes, 48 monoarmed telocentric (<fc>T</fc>) chromosomes and 140 microchromosomes. In genetic triploid specimens with 12.6–13.0 pg DNA content, the best informative metaphase with 402 chromosomes showed 120 biarmed <fc>M</fc> or <fc>SM</fc>, 72 monoarmed <fc>T</fc> chromosomes and 210 microchromosomes. The <fc>rDNA</fc> FISH detected a maximum 18 and 27 signals in the diploid and triploid <i>A. miakdoi</i>, respectively. The obtained findings thus corroborated a clear parallel between nuclear DNA contents and karyological or <fc>FISH</fc> profiles in the genetic diploid and triploid specimens, suggesting 1.5 times chromosome complements of diploid counterparts or three sets of homologues in the triploid sturgeons. Moreover, the estimated genome size and the observed molecular cytogenetic features in the diploid <i>A. mikadoi</i> strongly suggest that this species is a member of a functional tetraploid group recently proposed in the literature.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Molecular cytogenetic study on the ploidy status in Acipenser mikadoi</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Molecular cytogenetic study on the ploidy status in Acipenser mikadoi</title></titleInfo><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Zhou</namePart><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation><affiliation>He Zhou, Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University, 3‐1‐1 Minato‐cho, Hakodate, Hokkaido 041‐8611, Japan.E‐mail:</affiliation><affiliation>E-mail: xiaoben0603@yahoo.co.jp</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Fujimoto</namePart><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Adachi</namePart><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Abe</namePart><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Yamaha</namePart><affiliation>Nanae Fresh‐Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Nanae, Kameda, Hokkaido, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Arai</namePart><affiliation>Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2013-02</dateIssued><dateCreated encoding="w3cdtf">2012-10-19</dateCreated><dateCaptured encoding="w3cdtf">2011-12-08</dateCaptured><dateValid encoding="w3cdtf">2012-04-28</dateValid><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>The ploidy status of Acipenser mikadoi was examined using nuclear DNA contents, karyotypes and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. In flow‐cytometrically sorted specimens with 8.2–9.1 pg DNA content per somatic cell, i.e. genetic diploid, the best informative metaphase with 268 chromosomes had 80 biarmed meta‐ or submetacentric (M or SM) chromosomes, 48 monoarmed telocentric (T) chromosomes and 140 microchromosomes. In genetic triploid specimens with 12.6–13.0 pg DNA content, the best informative metaphase with 402 chromosomes showed 120 biarmed M or SM, 72 monoarmed T chromosomes and 210 microchromosomes. The rDNA FISH detected a maximum 18 and 27 signals in the diploid and triploid A. miakdoi, respectively. The obtained findings thus corroborated a clear parallel between nuclear DNA contents and karyological or FISH profiles in the genetic diploid and triploid specimens, suggesting 1.5 times chromosome complements of diploid counterparts or three sets of homologues in the triploid sturgeons. Moreover, the estimated genome size and the observed molecular cytogenetic features in the diploid A. mikadoi strongly suggest that this species is a member of a functional tetraploid group recently proposed in the literature.</abstract><note type="funding">Grants‐in‐Aid - No. K‐2; </note><note type="funding">Ministry of Education, Culture, Sports and Technology (MEXT)</note><note type="funding">Exploratory Research - No. 18658073; </note><note type="funding">Challenging Exploratory Research - No. 21658067; </note><note type="funding">Japan Society for the Promotion of Science (JSPS)</note><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><titleInfo type="abbreviated"><title>J. Appl. Ichthyol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Original Article</topic></subject><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2013</date><detail type="volume"><caption>vol.</caption><number>29</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>51</start><end>55</end><total>5</total></extent></part></relatedItem><identifier type="istex">1A04E51BC83828FAA431217CB4D61FA9F39F7745</identifier><identifier type="DOI">10.1111/jai.12109</identifier><identifier type="ArticleID">JAI12109</identifier><accessCondition type="use and reproduction" contentType="copyright">Journal compilation © 2013 Journal of Applied Ichthyology© 2012 Blackwell Verlag GmbH</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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