Isolation and partial characterization of proteins involved in maternal transfer of selenium in the western fence lizard (Sceloporus occidentalis)
Identifieur interne : 000393 ( Istex/Corpus ); précédent : 000392; suivant : 000394Isolation and partial characterization of proteins involved in maternal transfer of selenium in the western fence lizard (Sceloporus occidentalis)
Auteurs : Jason M. Unrine ; Brian P. Jackson ; William A. Hopkins ; Christopher RomanekSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2006-07.
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Abstract
Selenium from dietary exposure is efficiently transferred from mother to offspring in oviparous vertebrates, where it can cause severe teratogenic effects. We isolated and partially characterized proteins involved in maternal transfer of selenium in the oviparous lizard Sceloporus occidentalis using size‐exclusion chromatography, inductively coupled plasma–mass spectrometry, and polyacrylamide gel electrophoresis. Selenium from dietary selenomethionine exposure was incorporated into at least three egg proteins. One of these proteins was lipovitellin. The other two proteins may be part of a previously unknown mechanism of maternal transfer of Se that is independent of vitellogenesis or albumin secretion. Our results suggest at least three pathways for maternal transfer of Se in vertebrates that may vary in importance depending on the species.
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DOI: 10.1897/05-598R.1
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Drawer E, Aiken, South Carolina 29802, USA</affiliation><affiliation>Department of Geology, University of Georgia, Geography–Geology Building, Athens, Georgia 30602, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="shortCommunication"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2006-07</dateIssued><dateCaptured encoding="w3cdtf">2005-10-23</dateCaptured><dateValid encoding="w3cdtf">2005-12-16</dateValid><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="references">18</extent></physicalDescription><abstract lang="en">Selenium from dietary exposure is efficiently transferred from mother to offspring in oviparous vertebrates, where it can cause severe teratogenic effects. We isolated and partially characterized proteins involved in maternal transfer of selenium in the oviparous lizard Sceloporus occidentalis using size‐exclusion chromatography, inductively coupled plasma–mass spectrometry, and polyacrylamide gel electrophoresis. Selenium from dietary selenomethionine exposure was incorporated into at least three egg proteins. One of these proteins was lipovitellin. The other two proteins may be part of a previously unknown mechanism of maternal transfer of Se that is independent of vitellogenesis or albumin secretion. Our results suggest at least three pathways for maternal transfer of Se in vertebrates that may vary in importance depending on the species.</abstract><subject lang="en"><genre>keywords</genre><topic>Maternal transfer</topic><topic>Selenium</topic><topic>Sceloporus occidentalis</topic><topic>Inductively coupled plasma–mass spectrometry</topic><topic>Size‐exclusion chromatography</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Short Communication</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>1864</start><end>1867</end><total>4</total></extent></part></relatedItem><identifier type="istex">B0779E828415050B8BD0A15EA519CF0516582A4D</identifier><identifier type="DOI">10.1897/05-598R.1</identifier><identifier type="ArticleID">ETC5620250723</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2006 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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