Radioreceptor Assay for growth hormone in coho salmon (Oncorhynchus kisutch) and its application to the study of stunting
Identifieur interne : 001227 ( Istex/Checkpoint ); précédent : 001226; suivant : 001228Radioreceptor Assay for growth hormone in coho salmon (Oncorhynchus kisutch) and its application to the study of stunting
Auteurs : Elisabeth S. Gray [États-Unis] ; Graham Young [États-Unis, Nouvelle-Zélande] ; Howard A. Bern [États-Unis]Source :
- Journal of Experimental Zoology [ 0022-104X ] ; 1990-12.
Abstract
Binding sites for native chum salmon growth hormone (sGH) in coho salmon (Oncorhynchus kisutch) hepatic membranes were analyzed by radioreceptor assay. Displaceable (specific) binding represented up to 25% of total radiolabeled sGH added. Binding was dependent on buffer pH and membrane protein concentration, and was complete by 24 hours at 15°C. Specific binding was greatest in liver membranes, and was also detected in rr.uscle, ovary, gill, kidney, and brain. Scatchard analyses indicated a single class of hepatic binding sites that were specific for sGH. In stunts, abnormal seawater salmon with elevated plasma GEI levels and inhibited growth, specific binding of sGH to liver membranes was three times lower thiin in normal seawater smolts. The concentration of salmon GH binding sites was decreased in stunt livers by 60%, while their affinity for sGH was unchanged. Down‐regulation of hepatic GH receptors by high plasma GH levels may explain in part the low sGH binding in stunts.
Url:
DOI: 10.1002/jez.1402560308
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Radioreceptor Assay for growth hormone in coho salmon (Oncorhynchus kisutch) and its application to the study of stunting</title><author><name sortKey="Gray, Elisabeth S" sort="Gray, Elisabeth S" uniqKey="Gray E" first="Elisabeth S." last="Gray">Elisabeth S. Gray</name></author><author><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name></author><author><name sortKey="Bern, Howard A" sort="Bern, Howard A" uniqKey="Bern H" first="Howard A." last="Bern">Howard A. Bern</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:33129FA9B73543264FAD114FD20A63E180B64AE0</idno><date when="1990" year="1990">1990</date><idno type="doi">10.1002/jez.1402560308</idno><idno type="url">https://api.istex.fr/document/33129FA9B73543264FAD114FD20A63E180B64AE0/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000858</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000858</idno><idno type="wicri:Area/Istex/Curation">000856</idno><idno type="wicri:Area/Istex/Checkpoint">001227</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001227</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Radioreceptor Assay for growth hormone in coho salmon (Oncorhynchus kisutch) and its application to the study of stunting</title><author><name sortKey="Gray, Elisabeth S" sort="Gray, Elisabeth S" uniqKey="Gray E" first="Elisabeth S." last="Gray">Elisabeth S. Gray</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Integrative Biology and Cancer Research Laboratory, University of California, Berkeley</wicri:cityArea></affiliation></author><author><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Integrative Biology and Cancer Research Laboratory, University of California, Berkeley</wicri:cityArea></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Current Address: Dept. of Zoology, University of Otago, Dunedin</wicri:regionArea><wicri:noRegion>Dunedin</wicri:noRegion></affiliation></author><author><name sortKey="Bern, Howard A" sort="Bern, Howard A" uniqKey="Bern H" first="Howard A." last="Bern">Howard A. Bern</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Integrative Biology and Cancer Research Laboratory, University of California, Berkeley</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Zoology</title><title level="j" type="abbrev">J. Exp. Zool.</title><idno type="ISSN">0022-104X</idno><idno type="eISSN">1097-010X</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1990-12">1990-12</date><biblScope unit="volume">256</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="290">290</biblScope><biblScope unit="page" to="296">296</biblScope></imprint><idno type="ISSN">0022-104X</idno></series><idno type="istex">33129FA9B73543264FAD114FD20A63E180B64AE0</idno><idno type="DOI">10.1002/jez.1402560308</idno><idno type="ArticleID">JEZ1402560308</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-104X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Binding sites for native chum salmon growth hormone (sGH) in coho salmon (Oncorhynchus kisutch) hepatic membranes were analyzed by radioreceptor assay. Displaceable (specific) binding represented up to 25% of total radiolabeled sGH added. Binding was dependent on buffer pH and membrane protein concentration, and was complete by 24 hours at 15°C. Specific binding was greatest in liver membranes, and was also detected in rr.uscle, ovary, gill, kidney, and brain. Scatchard analyses indicated a single class of hepatic binding sites that were specific for sGH. In stunts, abnormal seawater salmon with elevated plasma GEI levels and inhibited growth, specific binding of sGH to liver membranes was three times lower thiin in normal seawater smolts. The concentration of salmon GH binding sites was decreased in stunt livers by 60%, while their affinity for sGH was unchanged. Down‐regulation of hepatic GH receptors by high plasma GH levels may explain in part the low sGH binding in stunts.</div></front></TEI><affiliations><list><country><li>Nouvelle-Zélande</li><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Gray, Elisabeth S" sort="Gray, Elisabeth S" uniqKey="Gray E" first="Elisabeth S." last="Gray">Elisabeth S. Gray</name></region><name sortKey="Bern, Howard A" sort="Bern, Howard A" uniqKey="Bern H" first="Howard A." last="Bern">Howard A. Bern</name><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name></country><country name="Nouvelle-Zélande"><noRegion><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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