Studies on purification and characterization of pituitary thyrotropin from black silver carp, Aristichthys nobilis
Identifieur interne : 001050 ( Istex/Checkpoint ); précédent : 001049; suivant : 001051Studies on purification and characterization of pituitary thyrotropin from black silver carp, Aristichthys nobilis
Auteurs : Subhadra Banerjee [République populaire de Chine] ; Partha P. Banerjee [République populaire de Chine] ; San-Tai Shen [République populaire de Chine] ; John Yuh-Lin Yu [République populaire de Chine]Source :
- Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology [ 0305-0491 ] ; 1994.
Abstract
Thyrotropin (TSH) was isolated from pituitaries of black silver carp (Aristichthys nobilis), designated as bscTSH. Molecular weight of bscTSH was 28 kDa as determined by gel-filtration chromatography. SDS-PAGE analysis revealed that bscTSH consisted of two subunits. Isoelectric point of bscTSH was at pHs from 7.8 to 8.4, and its sialic acid content was 0.86%. Amino acid composition of intact bscTSH was comparable to tetrapod TSHs. The isolated bscTSH was potent in stimulation of the circulating thyroxine level in common carp and loach, but was inactive in stimulation of their gonadal steroidogenesis.
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DOI: 10.1016/0305-0491(94)90057-4
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Banerjee</name></author><author><name sortKey="Shen, San Tai" sort="Shen, San Tai" uniqKey="Shen S" first="San-Tai" last="Shen">San-Tai Shen</name></author><author><name sortKey="Yuh Lin Yu, John" sort="Yuh Lin Yu, John" uniqKey="Yuh Lin Yu J" first="John" last="Yuh-Lin Yu">John Yuh-Lin Yu</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:99F25842A10A362764DFCC0BBB3B1D121F8F45EB</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1016/0305-0491(94)90057-4</idno><idno type="url">https://api.istex.fr/document/99F25842A10A362764DFCC0BBB3B1D121F8F45EB/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000940</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000940</idno><idno type="wicri:Area/Istex/Curation">000938</idno><idno type="wicri:Area/Istex/Checkpoint">001050</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001050</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Studies on purification and characterization of pituitary thyrotropin from black silver carp, Aristichthys nobilis</title><author><name sortKey="Banerjee, Subhadra" sort="Banerjee, Subhadra" uniqKey="Banerjee S" first="Subhadra" last="Banerjee">Subhadra Banerjee</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Endocrinology Laboratory, Institute of Zoology, Academia Sinica, Taipei, Taipei, Taiwan 115</wicri:regionArea><wicri:noRegion>Taiwan 115</wicri:noRegion></affiliation></author><author><name sortKey="Banerjee, Partha P" sort="Banerjee, Partha P" uniqKey="Banerjee P" first="Partha P." last="Banerjee">Partha P. Banerjee</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Endocrinology Laboratory, Institute of Zoology, Academia Sinica, Taipei, Taipei, Taiwan 115</wicri:regionArea><wicri:noRegion>Taiwan 115</wicri:noRegion></affiliation></author><author><name sortKey="Shen, San Tai" sort="Shen, San Tai" uniqKey="Shen S" first="San-Tai" last="Shen">San-Tai Shen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Endocrinology Laboratory, Institute of Zoology, Academia Sinica, Taipei, Taipei, Taiwan 115</wicri:regionArea><wicri:noRegion>Taiwan 115</wicri:noRegion></affiliation></author><author><name sortKey="Yuh Lin Yu, John" sort="Yuh Lin Yu, John" uniqKey="Yuh Lin Yu J" first="John" last="Yuh-Lin Yu">John Yuh-Lin Yu</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Endocrinology Laboratory, Institute of Zoology, Academia Sinica, Taipei, Taipei, Taiwan 115</wicri:regionArea><wicri:noRegion>Taiwan 115</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title><title level="j" type="abbrev">CBBOLD</title><idno type="ISSN">0305-0491</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">107</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="337">337</biblScope><biblScope unit="page" to="346">346</biblScope></imprint><idno type="ISSN">0305-0491</idno></series><idno type="istex">99F25842A10A362764DFCC0BBB3B1D121F8F45EB</idno><idno type="DOI">10.1016/0305-0491(94)90057-4</idno><idno type="PII">0305-0491(94)90057-4</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-0491</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Thyrotropin (TSH) was isolated from pituitaries of black silver carp (Aristichthys nobilis), designated as bscTSH. Molecular weight of bscTSH was 28 kDa as determined by gel-filtration chromatography. SDS-PAGE analysis revealed that bscTSH consisted of two subunits. Isoelectric point of bscTSH was at pHs from 7.8 to 8.4, and its sialic acid content was 0.86%. Amino acid composition of intact bscTSH was comparable to tetrapod TSHs. The isolated bscTSH was potent in stimulation of the circulating thyroxine level in common carp and loach, but was inactive in stimulation of their gonadal steroidogenesis.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Banerjee, Subhadra" sort="Banerjee, Subhadra" uniqKey="Banerjee S" first="Subhadra" last="Banerjee">Subhadra Banerjee</name></noRegion><name sortKey="Banerjee, Partha P" sort="Banerjee, Partha P" uniqKey="Banerjee P" first="Partha P." last="Banerjee">Partha P. Banerjee</name><name sortKey="Shen, San Tai" sort="Shen, San Tai" uniqKey="Shen S" first="San-Tai" last="Shen">San-Tai Shen</name><name sortKey="Yuh Lin Yu, John" sort="Yuh Lin Yu, John" uniqKey="Yuh Lin Yu J" first="John" last="Yuh-Lin Yu">John Yuh-Lin Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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