Application of non‐lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods
Identifieur interne : 000382 ( Istex/Checkpoint ); précédent : 000381; suivant : 000383Application of non‐lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods
Auteurs : R. T. Andvik [États-Unis] ; J. A. Vandehey [États-Unis] ; M. J. Fincel [États-Unis] ; W. E. French [États-Unis] ; K. N. Bertrand [États-Unis] ; S. R. Chipps [États-Unis] ; R. A. Klumb [États-Unis] ; B. D. S. Graeb [États-Unis]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2010-12.
Abstract
Traditional techniques for stable isotope analysis (SIA) generally require sacrificing animals to collect tissue samples; this can be problematic when studying diets of endangered species such as the pallid sturgeon Scaphirhynchus albus. Our objectives were to (i) determine if pectoral fin tissue (non‐lethal) could be a substitute for muscle tissue (lethal) in SIA of juvenile pallid sturgeon, and (ii) evaluate the influence of preservation techniques on stable isotope values. In the laboratory, individual juvenile pallid sturgeon were held for up to 186 day and fed chironomids, fish, or a commercially available pellet diet. Significant, positive relationships (r² ≥ 0.8) were observed between fin and muscle tissues for both δ15N and δ13C; in all samples isotopes were enriched in fins compared to muscle tissue. Chironomid and fish based diets of juvenile pallid sturgeon were distinguishable for fast growing fish (0.3 mm day−1) using stable δ15N and δ13C isotopes. Frozen and preserved fin tissue δ15N isotopes were strongly related (r2 = 0.89) but δ13C isotopes were weakly related (r2 = 0.16). Therefore, freezing is recommended for preservation of fin clips to avoid the confounding effect of enrichment by ethanol. This study demonstrates the utility of a non‐lethal technique to assess time integrated food habits of juvenile pallid sturgeon and should be applicable to other threatened or endangered species.
Url:
DOI: 10.1111/j.1439-0426.2010.01527.x
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:1BD479A0FDB60754ADFEBD4208F79BF9B0B1765ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Application of non‐lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods</title><author><name sortKey="Andvik, R T" sort="Andvik, R T" uniqKey="Andvik R" first="R. T." last="Andvik">R. T. Andvik</name></author><author><name sortKey="Vandehey, J A" sort="Vandehey, J A" uniqKey="Vandehey J" first="J. A." last="Vandehey">J. A. Vandehey</name></author><author><name sortKey="Fincel, M J" sort="Fincel, M J" uniqKey="Fincel M" first="M. J." last="Fincel">M. J. Fincel</name></author><author><name sortKey="French, W E" sort="French, W E" uniqKey="French W" first="W. E." last="French">W. E. French</name></author><author><name sortKey="Bertrand, K N" sort="Bertrand, K N" uniqKey="Bertrand K" first="K. N." last="Bertrand">K. N. Bertrand</name></author><author><name sortKey="Chipps, S R" sort="Chipps, S R" uniqKey="Chipps S" first="S. R." last="Chipps">S. R. Chipps</name></author><author><name sortKey="Klumb, R A" sort="Klumb, R A" uniqKey="Klumb R" first="R. A." last="Klumb">R. A. Klumb</name></author><author><name sortKey="Graeb, B D S" sort="Graeb, B D S" uniqKey="Graeb B" first="B. D. S." last="Graeb">B. D. S. Graeb</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1BD479A0FDB60754ADFEBD4208F79BF9B0B1765A</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1111/j.1439-0426.2010.01527.x</idno><idno type="url">https://api.istex.fr/document/1BD479A0FDB60754ADFEBD4208F79BF9B0B1765A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A35</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A35</idno><idno type="wicri:Area/Istex/Curation">000A33</idno><idno type="wicri:Area/Istex/Checkpoint">000382</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000382</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Application of non‐lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods</title><author><name sortKey="Andvik, R T" sort="Andvik, R T" uniqKey="Andvik R" first="R. T." last="Andvik">R. T. Andvik</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="Vandehey, J A" sort="Vandehey, J A" uniqKey="Vandehey J" first="J. A." last="Vandehey">J. A. Vandehey</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="Fincel, M J" sort="Fincel, M J" uniqKey="Fincel M" first="M. J." last="Fincel">M. J. Fincel</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>US Geological Survey, South Dakota Cooperative Fisheries and Wildlife Research Unit, Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="French, W E" sort="French, W E" uniqKey="French W" first="W. E." last="French">W. E. French</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="Bertrand, K N" sort="Bertrand, K N" uniqKey="Bertrand K" first="K. N." last="Bertrand">K. N. Bertrand</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="Chipps, S R" sort="Chipps, S R" uniqKey="Chipps S" first="S. R." last="Chipps">S. R. Chipps</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>US Geological Survey, South Dakota Cooperative Fisheries and Wildlife Research Unit, Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="Klumb, R A" sort="Klumb, R A" uniqKey="Klumb R" first="R. A." last="Klumb">R. A. Klumb</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>US Fish & Wildlife Service, Great Plains Fish & Wildlife Conservation Office, Pierre, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author><author><name sortKey="Graeb, B D S" sort="Graeb, B D S" uniqKey="Graeb B" first="B. D. S." last="Graeb">B. D. S. Graeb</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Wildlife and Fisheries Sciences, South Dakota State University, Brookings, SD</wicri:regionArea><placeName><region type="state">Dakota du Sud</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-12">2010-12</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="831">831</biblScope><biblScope unit="page" to="835">835</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">1BD479A0FDB60754ADFEBD4208F79BF9B0B1765A</idno><idno type="DOI">10.1111/j.1439-0426.2010.01527.x</idno><idno type="ArticleID">JAI1527</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Traditional techniques for stable isotope analysis (SIA) generally require sacrificing animals to collect tissue samples; this can be problematic when studying diets of endangered species such as the pallid sturgeon Scaphirhynchus albus. Our objectives were to (i) determine if pectoral fin tissue (non‐lethal) could be a substitute for muscle tissue (lethal) in SIA of juvenile pallid sturgeon, and (ii) evaluate the influence of preservation techniques on stable isotope values. In the laboratory, individual juvenile pallid sturgeon were held for up to 186 day and fed chironomids, fish, or a commercially available pellet diet. Significant, positive relationships (r² ≥ 0.8) were observed between fin and muscle tissues for both δ15N and δ13C; in all samples isotopes were enriched in fins compared to muscle tissue. Chironomid and fish based diets of juvenile pallid sturgeon were distinguishable for fast growing fish (0.3 mm day−1) using stable δ15N and δ13C isotopes. Frozen and preserved fin tissue δ15N isotopes were strongly related (r2 = 0.89) but δ13C isotopes were weakly related (r2 = 0.16). Therefore, freezing is recommended for preservation of fin clips to avoid the confounding effect of enrichment by ethanol. This study demonstrates the utility of a non‐lethal technique to assess time integrated food habits of juvenile pallid sturgeon and should be applicable to other threatened or endangered species.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Dakota du Sud</li></region></list><tree><country name="États-Unis"><region name="Dakota du Sud"><name sortKey="Andvik, R T" sort="Andvik, R T" uniqKey="Andvik R" first="R. T." last="Andvik">R. T. Andvik</name></region><name sortKey="Bertrand, K N" sort="Bertrand, K N" uniqKey="Bertrand K" first="K. N." last="Bertrand">K. N. Bertrand</name><name sortKey="Chipps, S R" sort="Chipps, S R" uniqKey="Chipps S" first="S. R." last="Chipps">S. R. Chipps</name><name sortKey="Fincel, M J" sort="Fincel, M J" uniqKey="Fincel M" first="M. J." last="Fincel">M. J. Fincel</name><name sortKey="French, W E" sort="French, W E" uniqKey="French W" first="W. E." last="French">W. E. French</name><name sortKey="Graeb, B D S" sort="Graeb, B D S" uniqKey="Graeb B" first="B. D. S." last="Graeb">B. D. S. Graeb</name><name sortKey="Klumb, R A" sort="Klumb, R A" uniqKey="Klumb R" first="R. A." last="Klumb">R. A. Klumb</name><name sortKey="Vandehey, J A" sort="Vandehey, J A" uniqKey="Vandehey J" first="J. A." last="Vandehey">J. A. Vandehey</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000382 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000382 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:1BD479A0FDB60754ADFEBD4208F79BF9B0B1765A
|texte= Application of non‐lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods
}}
| This area was generated with Dilib version V0.6.27. |  |