Fertilization by refrigerator stored sperm of the Indian major carp, Labeo calbasu (Hamilton, 1822)
Identifieur interne : 000054 ( Istex/Checkpoint ); précédent : 000053; suivant : 000055Fertilization by refrigerator stored sperm of the Indian major carp, Labeo calbasu (Hamilton, 1822)
Auteurs : Md Mahbubul Hassan [Bangladesh] ; Md Nahiduzzaman [Bangladesh] ; Shaheed Nasrullah Al Mamun [Bangladesh] ; Md Abu Taher [Bangladesh] ; Mostafa Ali Reza Hossain [Bangladesh]Source :
- Aquaculture Research [ 1355-557X ] ; 2013-12.
Abstract
This study reports on the spermatological properties, and on the development of a protocol for refrigerator storage (4°C) of Labeo calbasu (Hamilton, 1822) sperm for artificial breeding. Volume, motility, concentration and pH of the freshly collected sperm were 2.21 ± 0.53 (μL g−1 of fish weight) (mean ± SD), 95 ± 1 (%), 1.93 ± 0.44 × 109 (cells mL−1) and 7.56 ± 0.17 respectively. Sperm activation was evaluated at different osmolalities of NaCl solution, and motility ceased completely when osmolality of the extender was ≥287 mOsmol kg−1. Sperm retained motility for 24, 72 and 108 h, after refrigerator storage when sperm were undiluted, suspended in Alsever's solution and suspended in Alsever's solution containing 5% methanol respectively. Fertilization rate of fresh eggs with sperm stored at 4°C in Alsever's solution and Alsever's solution containing 5% methanol was 77% and 60% with a hatching rate of 60% and 43% respectively. The fertilization and hatching success of the stored sperm suggests potential to use refrigeration for transporting genetic material to hatcheries for artificial breeding of L. calbasu in Bangladesh.
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DOI: 10.1111/j.1365-2109.2012.03214.x
Affiliations:
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<front><div type="abstract">This study reports on the spermatological properties, and on the development of a protocol for refrigerator storage (4°C) of Labeo calbasu (Hamilton, 1822) sperm for artificial breeding. Volume, motility, concentration and pH of the freshly collected sperm were 2.21 ± 0.53 (μL g−1 of fish weight) (mean ± SD), 95 ± 1 (%), 1.93 ± 0.44 × 109 (cells mL−1) and 7.56 ± 0.17 respectively. Sperm activation was evaluated at different osmolalities of NaCl solution, and motility ceased completely when osmolality of the extender was ≥287 mOsmol kg−1. Sperm retained motility for 24, 72 and 108 h, after refrigerator storage when sperm were undiluted, suspended in Alsever's solution and suspended in Alsever's solution containing 5% methanol respectively. Fertilization rate of fresh eggs with sperm stored at 4°C in Alsever's solution and Alsever's solution containing 5% methanol was 77% and 60% with a hatching rate of 60% and 43% respectively. The fertilization and hatching success of the stored sperm suggests potential to use refrigeration for transporting genetic material to hatcheries for artificial breeding of L. calbasu in Bangladesh.</div>
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