Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds.
Identifieur interne : 001349 ( Main/Exploration ); précédent : 001348; suivant : 001350Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds.
Auteurs : Jingli Yang [République populaire de Chine] ; Jaeseon Yi ; Chuanping Yang ; Chenghao LiSource :
- Tree physiology [ 1758-4469 ] ; 2013.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Développement des plantes (génétique), Germination (MeSH), Graines (MeSH), Gènes de plante (MeSH), Kanamycine (MeSH), Pousses de plante (MeSH), Racines de plante (MeSH), Résistance aux substances (génétique), Salix (génétique), Transformation génétique (MeSH), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Drug Resistance (genetics), Genes, Plant (MeSH), Genetic Vectors (MeSH), Germination (MeSH), Kanamycin (MeSH), Plant Development (genetics), Plant Roots (MeSH), Plant Shoots (MeSH), Plants, Genetically Modified (MeSH), Salix (genetics), Seeds (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical : Kanamycin.
- genetics : Agrobacterium tumefaciens, Drug Resistance, Plant Development, Salix.
- Genes, Plant, Genetic Vectors, Germination, Plant Roots, Plant Shoots, Plants, Genetically Modified, Seeds, Transformation, Genetic.
Abstract
An Agrobacterium tumefaciens-mediated transformation method was developed for Salix matsudana Koidz. using mature seeds as starting material. Multiple shoots were induced directly from embryonic shoot apices of germinating seeds. Although thidiazuron, 6-benzylaminopurine and zeatin induced multiple shoot induction with high frequency, zeatin (4.5 μM) was more effective for elongation of shoots and roots. The binary vector pCAMBIA1303, which contained neomycin phosphotransferase as a selectable marker gene and β-glucuronidase as a reporter gene, was used for transformation. Factors affecting transformation efficiency were examined for optimization of the procedure. Up to 35 of 180 seeds regenerated kanamycin-resistant shoots under optimal transformation conditions as follows: seeds were precultured for 4 days, apices of embryonic shoots were removed and infected with A. tumefaciens strain LBA4404 grown to a cell density equivalent (OD600) of 0.6, and then the infected explants were cultivated at 21 °C for 4 days. Storage of seeds at -20 °C for as long as 3 years had no significant effect on the induction of kanamycin-resistant shoots. Using this method, transgenic plants were obtained within ∼5 months with a transformation frequency of 7.2%. Analysis by polymerase chain reaction (PCR) showed that 36.4-93.8% of plants from all 13 tested kanamycin-resistant lines were PCR positive. Several 'escapes' were eliminated by a second round of selection. PCR, Southern blot and reverse transcriptase-PCR analyses of selected transgenic individuals 2 years after cutting propagation confirmed the successful generation of stable transformants. Our method, which minimizes the duration of axenic culture, may provide an alternative procedure for transformation of other recalcitrant Salix species.
DOI: 10.1093/treephys/tpt038
PubMed: 23771952
Affiliations:
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last="Yang">Chuanping Yang</name></author><author><name sortKey="Li, Chenghao" sort="Li, Chenghao" uniqKey="Li C" first="Chenghao" last="Li">Chenghao Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23771952</idno><idno type="pmid">23771952</idno><idno type="doi">10.1093/treephys/tpt038</idno><idno type="wicri:Area/Main/Corpus">001257</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001257</idno><idno type="wicri:Area/Main/Curation">001257</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001257</idno><idno type="wicri:Area/Main/Exploration">001257</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds.</title><author><name sortKey="Yang, Jingli" sort="Yang, Jingli" uniqKey="Yang J" first="Jingli" last="Yang">Jingli Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Yi, Jaeseon" sort="Yi, Jaeseon" uniqKey="Yi J" first="Jaeseon" last="Yi">Jaeseon Yi</name></author><author><name sortKey="Yang, Chuanping" sort="Yang, Chuanping" uniqKey="Yang C" first="Chuanping" last="Yang">Chuanping Yang</name></author><author><name sortKey="Li, Chenghao" sort="Li, Chenghao" uniqKey="Li C" first="Chenghao" last="Li">Chenghao Li</name></author></analytic><series><title level="j">Tree physiology</title><idno 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(MeSH)</term><term>Résistance aux substances (génétique)</term><term>Salix (génétique)</term><term>Transformation génétique (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Kanamycin</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Drug Resistance</term><term>Plant Development</term><term>Salix</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Développement des plantes</term><term>Résistance aux substances</term><term>Salix</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Plant</term><term>Genetic Vectors</term><term>Germination</term><term>Plant Roots</term><term>Plant Shoots</term><term>Plants, Genetically Modified</term><term>Seeds</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Germination</term><term>Graines</term><term>Gènes de plante</term><term>Kanamycine</term><term>Pousses de plante</term><term>Racines de plante</term><term>Transformation génétique</term><term>Vecteurs génétiques</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An Agrobacterium tumefaciens-mediated transformation method was developed for Salix matsudana Koidz. using mature seeds as starting material. Multiple shoots were induced directly from embryonic shoot apices of germinating seeds. Although thidiazuron, 6-benzylaminopurine and zeatin induced multiple shoot induction with high frequency, zeatin (4.5 μM) was more effective for elongation of shoots and roots. The binary vector pCAMBIA1303, which contained neomycin phosphotransferase as a selectable marker gene and β-glucuronidase as a reporter gene, was used for transformation. Factors affecting transformation efficiency were examined for optimization of the procedure. Up to 35 of 180 seeds regenerated kanamycin-resistant shoots under optimal transformation conditions as follows: seeds were precultured for 4 days, apices of embryonic shoots were removed and infected with A. tumefaciens strain LBA4404 grown to a cell density equivalent (OD600) of 0.6, and then the infected explants were cultivated at 21 °C for 4 days. Storage of seeds at -20 °C for as long as 3 years had no significant effect on the induction of kanamycin-resistant shoots. Using this method, transgenic plants were obtained within ∼5 months with a transformation frequency of 7.2%. Analysis by polymerase chain reaction (PCR) showed that 36.4-93.8% of plants from all 13 tested kanamycin-resistant lines were PCR positive. Several 'escapes' were eliminated by a second round of selection. PCR, Southern blot and reverse transcriptase-PCR analyses of selected transgenic individuals 2 years after cutting propagation confirmed the successful generation of stable transformants. Our method, which minimizes the duration of axenic culture, may provide an alternative procedure for transformation of other recalcitrant Salix species. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23771952</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>07</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>6</Issue><PubDate><Year>2013</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds.</ArticleTitle><Pagination><MedlinePgn>628-39</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpt038</ELocationID><Abstract><AbstractText>An Agrobacterium tumefaciens-mediated transformation method was developed for Salix matsudana Koidz. using mature seeds as starting material. Multiple shoots were induced directly from embryonic shoot apices of germinating seeds. Although thidiazuron, 6-benzylaminopurine and zeatin induced multiple shoot induction with high frequency, zeatin (4.5 μM) was more effective for elongation of shoots and roots. The binary vector pCAMBIA1303, which contained neomycin phosphotransferase as a selectable marker gene and β-glucuronidase as a reporter gene, was used for transformation. Factors affecting transformation efficiency were examined for optimization of the procedure. Up to 35 of 180 seeds regenerated kanamycin-resistant shoots under optimal transformation conditions as follows: seeds were precultured for 4 days, apices of embryonic shoots were removed and infected with A. tumefaciens strain LBA4404 grown to a cell density equivalent (OD600) of 0.6, and then the infected explants were cultivated at 21 °C for 4 days. Storage of seeds at -20 °C for as long as 3 years had no significant effect on the induction of kanamycin-resistant shoots. Using this method, transgenic plants were obtained within ∼5 months with a transformation frequency of 7.2%. Analysis by polymerase chain reaction (PCR) showed that 36.4-93.8% of plants from all 13 tested kanamycin-resistant lines were PCR positive. Several 'escapes' were eliminated by a second round of selection. PCR, Southern blot and reverse transcriptase-PCR analyses of selected transgenic individuals 2 years after cutting propagation confirmed the successful generation of stable transformants. Our method, which minimizes the duration of axenic culture, may provide an alternative procedure for transformation of other recalcitrant Salix species. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jingli</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yi</LastName><ForeName>Jaeseon</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chuanping</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chenghao</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>06</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>59-01-8</RegistryNumber><NameOfSubstance UI="D007612">Kanamycin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004351" MajorTopicYN="N">Drug Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018525" MajorTopicYN="N">Germination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007612" MajorTopicYN="N">Kanamycin</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D063245" MajorTopicYN="N">Plant Development</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="Y">Seeds</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">chimaeric</Keyword><Keyword MajorTopicYN="N">embryonic shoot apex</Keyword><Keyword MajorTopicYN="N">mature seed</Keyword><Keyword MajorTopicYN="N">multiple shoot</Keyword><Keyword MajorTopicYN="N">willow</Keyword><Keyword MajorTopicYN="N">woody plant transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23771952</ArticleId><ArticleId IdType="pii">tpt038</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpt038</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Li, Chenghao" sort="Li, Chenghao" uniqKey="Li C" first="Chenghao" last="Li">Chenghao Li</name><name sortKey="Yang, Chuanping" sort="Yang, Chuanping" uniqKey="Yang C" first="Chuanping" last="Yang">Chuanping Yang</name><name sortKey="Yi, Jaeseon" sort="Yi, Jaeseon" uniqKey="Yi J" first="Jaeseon" last="Yi">Jaeseon Yi</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yang, Jingli" sort="Yang, Jingli" uniqKey="Yang J" first="Jingli" last="Yang">Jingli Yang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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