Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes.
Identifieur interne : 000977 ( Main/Exploration ); précédent : 000976; suivant : 000978Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes.
Auteurs : Nidhal Soualeh [France] ; Aliçia Stiévenard [France] ; Elie Baudelaire [France] ; Rachid Soulimani [France] ; Jaouad Bouayed [France]Source :
- International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition [ 0300-9831 ] ; 2017.
Abstract
In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50-100 µm, 100-180 µm and 180-315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50-100 µm and 100-180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100-180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.
DOI: 10.1024/0300-9831/a000506
PubMed: 30816796
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Improvement of cytoprotective and antioxidant activity of <b><i>Rosa canina</i></b> L. and <b><i>Salix alba</i></b> L. by controlled differential sieving process against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in mouse primary splenocytes.</title><author><name sortKey="Soualeh, Nidhal" sort="Soualeh, Nidhal" uniqKey="Soualeh N" first="Nidhal" last="Soualeh">Nidhal Soualeh</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Stievenard, Alicia" sort="Stievenard, Alicia" uniqKey="Stievenard A" first="Aliçia" last="Stiévenard">Aliçia Stiévenard</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Baudelaire, Elie" sort="Baudelaire, Elie" uniqKey="Baudelaire E" first="Elie" last="Baudelaire">Elie Baudelaire</name><affiliation wicri:level="3"><nlm:affiliation>2 AGRITECH, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2 AGRITECH, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author><author><name sortKey="Soulimani, Rachid" sort="Soulimani, Rachid" uniqKey="Soulimani R" first="Rachid" last="Soulimani">Rachid Soulimani</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Bouayed, Jaouad" sort="Bouayed, Jaouad" uniqKey="Bouayed J" first="Jaouad" last="Bouayed">Jaouad Bouayed</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:30816796</idno><idno type="pmid">30816796</idno><idno type="doi">10.1024/0300-9831/a000506</idno><idno type="wicri:Area/Main/Corpus">000512</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000512</idno><idno type="wicri:Area/Main/Curation">000512</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000512</idno><idno type="wicri:Area/Main/Exploration">000512</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Improvement of cytoprotective and antioxidant activity of <b><i>Rosa canina</i></b> L. and <b><i>Salix alba</i></b> L. by controlled differential sieving process against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in mouse primary splenocytes.</title><author><name sortKey="Soualeh, Nidhal" sort="Soualeh, Nidhal" uniqKey="Soualeh N" first="Nidhal" last="Soualeh">Nidhal Soualeh</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Stievenard, Alicia" sort="Stievenard, Alicia" uniqKey="Stievenard A" first="Aliçia" last="Stiévenard">Aliçia Stiévenard</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Baudelaire, Elie" sort="Baudelaire, Elie" uniqKey="Baudelaire E" first="Elie" last="Baudelaire">Elie Baudelaire</name><affiliation wicri:level="3"><nlm:affiliation>2 AGRITECH, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2 AGRITECH, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author><author><name sortKey="Soulimani, Rachid" sort="Soulimani, Rachid" uniqKey="Soulimani R" first="Rachid" last="Soulimani">Rachid Soulimani</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Bouayed, Jaouad" sort="Bouayed, Jaouad" uniqKey="Bouayed J" first="Jaouad" last="Bouayed">Jaouad Bouayed</name><affiliation wicri:level="4"><nlm:affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Metz</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author></analytic><series><title level="j">International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition</title><idno type="ISSN">0300-9831</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><b></b> In this study, cytoprotective and antioxidant activities of <i>Rosa canina</i> (RC) and <i>Salix alba</i> (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, <i>versus</i> a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50-100 µm, 100-180 µm and 180-315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H<sub>2</sub>O<sub>2</sub>-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50-100 µm and 100-180 µm, both <i>p</i> < 0.001), and with the intermediate powder for RC (i. e., 100-180 µm, <i>p</i> < 0.05) <i>versus</i> HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H<sub>2</sub>O<sub>2</sub>, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30816796</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0300-9831</ISSN><JournalIssue CitedMedium="Print"><Volume>87</Volume><Issue>3-4</Issue><PubDate><Year>2017</Year><Month>May</Month></PubDate></JournalIssue><Title>International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition</Title><ISOAbbreviation>Int J Vitam Nutr Res</ISOAbbreviation></Journal><ArticleTitle>Improvement of cytoprotective and antioxidant activity of <b><i>Rosa canina</i></b> L. and <b><i>Salix alba</i></b> L. by controlled differential sieving process against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in mouse primary splenocytes.</ArticleTitle><Pagination><MedlinePgn>191-200</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1024/0300-9831/a000506</ELocationID><Abstract><AbstractText><b></b> In this study, cytoprotective and antioxidant activities of <i>Rosa canina</i> (RC) and <i>Salix alba</i> (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, <i>versus</i> a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50-100 µm, 100-180 µm and 180-315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H<sub>2</sub>O<sub>2</sub>-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50-100 µm and 100-180 µm, both <i>p</i> < 0.001), and with the intermediate powder for RC (i. e., 100-180 µm, <i>p</i> < 0.05) <i>versus</i> HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H<sub>2</sub>O<sub>2</sub>, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Soualeh</LastName><ForeName>Nidhal</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stiévenard</LastName><ForeName>Aliçia</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baudelaire</LastName><ForeName>Elie</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>2 AGRITECH, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soulimani</LastName><ForeName>Rachid</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouayed</LastName><ForeName>Jaouad</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>1 Université de Lorraine, Neurotoxicologie Alimentaire et Bioactivité, Metz, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int J Vitam Nutr Res</MedlineTA><NlmUniqueID>1273304</NlmUniqueID><ISSNLinking>0300-9831</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N"> L.</Keyword><Keyword MajorTopicYN="N">Controlled differential sieving process</Keyword><Keyword MajorTopicYN="N">antioxidant activity</Keyword><Keyword MajorTopicYN="N">cytoprotective activity</Keyword><Keyword MajorTopicYN="N">splenocytes</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30816796</ArticleId><ArticleId IdType="doi">10.1024/0300-9831/a000506</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Metz</li><li>Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="France"><region name="Grand Est"><name sortKey="Soualeh, Nidhal" sort="Soualeh, Nidhal" uniqKey="Soualeh N" first="Nidhal" last="Soualeh">Nidhal Soualeh</name></region><name sortKey="Baudelaire, Elie" sort="Baudelaire, Elie" uniqKey="Baudelaire E" first="Elie" last="Baudelaire">Elie Baudelaire</name><name sortKey="Bouayed, Jaouad" sort="Bouayed, Jaouad" uniqKey="Bouayed J" first="Jaouad" last="Bouayed">Jaouad Bouayed</name><name sortKey="Soulimani, Rachid" sort="Soulimani, Rachid" uniqKey="Soulimani R" first="Rachid" last="Soulimani">Rachid Soulimani</name><name sortKey="Stievenard, Alicia" sort="Stievenard, Alicia" uniqKey="Stievenard A" first="Aliçia" last="Stiévenard">Aliçia Stiévenard</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/WillowV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000977 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000977 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= WillowV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30816796
|texte= Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30816796" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a WillowV1
| This area was generated with Dilib version V0.6.37. |  |