Quantitative multi-locus metabarcoding and waggle dance interpretation reveal honey bee spring foraging patterns in Midwest agroecosystems.
Identifieur interne : 000417 ( Main/Exploration ); précédent : 000416; suivant : 000418Quantitative multi-locus metabarcoding and waggle dance interpretation reveal honey bee spring foraging patterns in Midwest agroecosystems.
Auteurs : Rodney T. Richardson [États-Unis] ; Hailey R. Curtis [États-Unis] ; Emma G. Matcham [États-Unis] ; Chia-Hua Lin [États-Unis] ; Sreelakshmi Suresh [États-Unis] ; Douglas B. Sponsler [États-Unis] ; Luke E. Hearon [États-Unis] ; Reed M. Johnson [États-Unis]Source :
- Molecular ecology [ 1365-294X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Pollen.
- physiologie : Abeilles.
- Animaux, Bases de données d'acides nucléiques, Codage à barres de l'ADN pour la taxonomie, Comportement animal, Comportement appétitif, Ohio, Saisons.
English descriptors
- KwdEn :
- MESH :
- geographic : Ohio.
- genetics : Pollen.
- physiology : Bees.
- Animals, Appetitive Behavior, Behavior, Animal, DNA Barcoding, Taxonomic, Databases, Nucleic Acid, Seasons.
Abstract
We explored the pollen foraging behaviour of honey bee colonies situated in the corn and soybean dominated agroecosystems of central Ohio over a month-long period using both pollen metabarcoding and waggle dance inference of spatial foraging patterns. For molecular pollen analysis, we developed simple and cost-effective laboratory and bioinformatics methods. Targeting four plant barcode loci (ITS2, rbcL, trnL and trnH), we implemented metabarcoding library preparation and dual-indexing protocols designed to minimize amplification biases and index mistagging events. We constructed comprehensive, curated reference databases for hierarchical taxonomic classification of metabarcoding data and used these databases to train the metaxa2 DNA sequence classifier. Comparisons between morphological and molecular palynology provide strong support for the quantitative potential of multi-locus metabarcoding. Results revealed consistent foraging habits between locations and show clear trends in the phenological progression of honey bee spring foraging in these agricultural areas. Our data suggest that three key taxa, woody Rosaceae such as pome fruits and hawthorns, Salix, and Trifolium provided the majority of pollen nutrition during the study. Spatially, these foraging patterns were associated with a significant preference for forests and tree lines relative to herbaceous land cover and nonflowering crop fields.
DOI: 10.1111/mec.14975
PubMed: 30549365
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Richardson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Entomology, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Curtis, Hailey R" sort="Curtis, Hailey R" uniqKey="Curtis H" first="Hailey R" last="Curtis">Hailey R. Curtis</name><affiliation wicri:level="2"><nlm:affiliation>College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>College of Veterinary Medicine, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Matcham, Emma G" sort="Matcham, Emma G" uniqKey="Matcham E" first="Emma G" last="Matcham">Emma G. Matcham</name><affiliation wicri:level="2"><nlm:affiliation>Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Horticulture and Crop Science, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Lin, Chia Hua" sort="Lin, Chia Hua" uniqKey="Lin C" first="Chia-Hua" last="Lin">Chia-Hua Lin</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Entomology, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Suresh, Sreelakshmi" sort="Suresh, Sreelakshmi" uniqKey="Suresh S" first="Sreelakshmi" last="Suresh">Sreelakshmi Suresh</name><affiliation wicri:level="2"><nlm:affiliation>Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Sponsler, Douglas B" sort="Sponsler, Douglas B" uniqKey="Sponsler D" first="Douglas B" last="Sponsler">Douglas B. Sponsler</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, Pennsylvania State University, University Park, Pennsylvania.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Entomology, Pennsylvania State University, University Park</wicri:cityArea></affiliation></author><author><name sortKey="Hearon, Luke E" sort="Hearon, Luke E" uniqKey="Hearon L" first="Luke E" last="Hearon">Luke E. Hearon</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Entomology, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Johnson, Reed M" sort="Johnson, Reed M" uniqKey="Johnson R" first="Reed M" last="Johnson">Reed M. Johnson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Entomology, The Ohio State University, Columbus</wicri:cityArea></affiliation></author></analytic><series><title level="j">Molecular ecology</title><idno type="eISSN">1365-294X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Appetitive Behavior (MeSH)</term><term>Bees (physiology)</term><term>Behavior, Animal (MeSH)</term><term>DNA Barcoding, Taxonomic (MeSH)</term><term>Databases, Nucleic Acid (MeSH)</term><term>Ohio (MeSH)</term><term>Pollen (genetics)</term><term>Seasons (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Abeilles (physiologie)</term><term>Animaux (MeSH)</term><term>Bases de données d'acides nucléiques (MeSH)</term><term>Codage à barres de l'ADN pour la taxonomie (MeSH)</term><term>Comportement animal (MeSH)</term><term>Comportement appétitif (MeSH)</term><term>Ohio (MeSH)</term><term>Pollen (génétique)</term><term>Saisons (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Ohio</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Abeilles</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Bees</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Appetitive Behavior</term><term>Behavior, Animal</term><term>DNA Barcoding, Taxonomic</term><term>Databases, Nucleic Acid</term><term>Seasons</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bases de données d'acides nucléiques</term><term>Codage à barres de l'ADN pour la taxonomie</term><term>Comportement animal</term><term>Comportement appétitif</term><term>Ohio</term><term>Saisons</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We explored the pollen foraging behaviour of honey bee colonies situated in the corn and soybean dominated agroecosystems of central Ohio over a month-long period using both pollen metabarcoding and waggle dance inference of spatial foraging patterns. For molecular pollen analysis, we developed simple and cost-effective laboratory and bioinformatics methods. Targeting four plant barcode loci (ITS2, rbcL, trnL and trnH), we implemented metabarcoding library preparation and dual-indexing protocols designed to minimize amplification biases and index mistagging events. We constructed comprehensive, curated reference databases for hierarchical taxonomic classification of metabarcoding data and used these databases to train the metaxa2 DNA sequence classifier. Comparisons between morphological and molecular palynology provide strong support for the quantitative potential of multi-locus metabarcoding. Results revealed consistent foraging habits between locations and show clear trends in the phenological progression of honey bee spring foraging in these agricultural areas. Our data suggest that three key taxa, woody Rosaceae such as pome fruits and hawthorns, Salix, and Trifolium provided the majority of pollen nutrition during the study. Spatially, these foraging patterns were associated with a significant preference for forests and tree lines relative to herbaceous land cover and nonflowering crop fields.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30549365</PMID><DateCompleted><Year>2019</Year><Month>10</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1365-294X</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>3</Issue><PubDate><Year>2019</Year><Month>02</Month></PubDate></JournalIssue><Title>Molecular ecology</Title><ISOAbbreviation>Mol Ecol</ISOAbbreviation></Journal><ArticleTitle>Quantitative multi-locus metabarcoding and waggle dance interpretation reveal honey bee spring foraging patterns in Midwest agroecosystems.</ArticleTitle><Pagination><MedlinePgn>686-697</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/mec.14975</ELocationID><Abstract><AbstractText>We explored the pollen foraging behaviour of honey bee colonies situated in the corn and soybean dominated agroecosystems of central Ohio over a month-long period using both pollen metabarcoding and waggle dance inference of spatial foraging patterns. For molecular pollen analysis, we developed simple and cost-effective laboratory and bioinformatics methods. Targeting four plant barcode loci (ITS2, rbcL, trnL and trnH), we implemented metabarcoding library preparation and dual-indexing protocols designed to minimize amplification biases and index mistagging events. We constructed comprehensive, curated reference databases for hierarchical taxonomic classification of metabarcoding data and used these databases to train the metaxa2 DNA sequence classifier. Comparisons between morphological and molecular palynology provide strong support for the quantitative potential of multi-locus metabarcoding. Results revealed consistent foraging habits between locations and show clear trends in the phenological progression of honey bee spring foraging in these agricultural areas. Our data suggest that three key taxa, woody Rosaceae such as pome fruits and hawthorns, Salix, and Trifolium provided the majority of pollen nutrition during the study. Spatially, these foraging patterns were associated with a significant preference for forests and tree lines relative to herbaceous land cover and nonflowering crop fields.</AbstractText><CopyrightInformation>© 2018 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Richardson</LastName><ForeName>Rodney T</ForeName><Initials>RT</Initials><Identifier Source="ORCID">0000-0002-4443-1705</Identifier><AffiliationInfo><Affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Curtis</LastName><ForeName>Hailey R</ForeName><Initials>HR</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Matcham</LastName><ForeName>Emma G</ForeName><Initials>EG</Initials><AffiliationInfo><Affiliation>Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Chia-Hua</ForeName><Initials>CH</Initials><AffiliationInfo><Affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suresh</LastName><ForeName>Sreelakshmi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sponsler</LastName><ForeName>Douglas B</ForeName><Initials>DB</Initials><AffiliationInfo><Affiliation>Department of Entomology, Pennsylvania State University, University Park, Pennsylvania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hearon</LastName><ForeName>Luke E</ForeName><Initials>LE</Initials><AffiliationInfo><Affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>Reed M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Department of Entomology, The Ohio State University, Columbus, Ohio.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Project Apis m.-Costco Honey Bee Biology Fellowship</Agency><Country>International</Country></Grant><Grant><Agency>Pollinator Partnership Corn Dust Research Consortium grant</Agency><Country>International</Country></Grant><Grant><GrantID>OHO01277</GrantID><Agency>state and federal funds appropriated to The Ohio State University, Ohio Agricultural Research and Development Center</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Ecol</MedlineTA><NlmUniqueID>9214478</NlmUniqueID><ISSNLinking>0962-1083</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001070" MajorTopicYN="Y">Appetitive Behavior</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001516" MajorTopicYN="N">Bees</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001522" MajorTopicYN="Y">Behavior, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058893" MajorTopicYN="N">DNA Barcoding, Taxonomic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030561" MajorTopicYN="N">Databases, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009820" MajorTopicYN="N" Type="Geographic">Ohio</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012621" MajorTopicYN="N">Seasons</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">hierarchical classification</Keyword><Keyword MajorTopicYN="Y">molecular palynology</Keyword><Keyword MajorTopicYN="Y">pollinator nutrition</Keyword><Keyword MajorTopicYN="Y">quantitative metabarcoding</Keyword><Keyword MajorTopicYN="Y">waggle dance</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>09</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>11</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30549365</ArticleId><ArticleId IdType="doi">10.1111/mec.14975</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Richardson, Rodney T" sort="Richardson, Rodney T" uniqKey="Richardson R" first="Rodney T" last="Richardson">Rodney T. Richardson</name></region><name sortKey="Curtis, Hailey R" sort="Curtis, Hailey R" uniqKey="Curtis H" first="Hailey R" last="Curtis">Hailey R. Curtis</name><name sortKey="Hearon, Luke E" sort="Hearon, Luke E" uniqKey="Hearon L" first="Luke E" last="Hearon">Luke E. Hearon</name><name sortKey="Johnson, Reed M" sort="Johnson, Reed M" uniqKey="Johnson R" first="Reed M" last="Johnson">Reed M. Johnson</name><name sortKey="Lin, Chia Hua" sort="Lin, Chia Hua" uniqKey="Lin C" first="Chia-Hua" last="Lin">Chia-Hua Lin</name><name sortKey="Matcham, Emma G" sort="Matcham, Emma G" uniqKey="Matcham E" first="Emma G" last="Matcham">Emma G. Matcham</name><name sortKey="Sponsler, Douglas B" sort="Sponsler, Douglas B" uniqKey="Sponsler D" first="Douglas B" last="Sponsler">Douglas B. Sponsler</name><name sortKey="Suresh, Sreelakshmi" sort="Suresh, Sreelakshmi" uniqKey="Suresh S" first="Sreelakshmi" last="Suresh">Sreelakshmi Suresh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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