First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA.
Identifieur interne : 000206 ( Main/Exploration ); précédent : 000205; suivant : 000207First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA.
Auteurs : Jonathan Willow [Estonie] ; Liina Soonvald [Estonie] ; Silva Sulg [Estonie] ; Riina Kaasik [Estonie] ; Ana Isabel Silva [Pays-Bas] ; Clauvis Nji Tizi Taning [Belgique] ; Olivier Christiaens [Belgique] ; Guy Smagghe [Belgique] ; Eve Veromann [Estonie]Source :
- Insects [ 2075-4450 ] ; 2020.
Abstract
Spray-induced gene silencing (SIGS) is a potential strategy for agricultural pest management, whereby nucleotide sequence-specific double-stranded RNA (dsRNA) can be sprayed onto a crop; the desired effect being a consumption of dsRNA by the target pest, and subsequent gene silencing-induced mortality. Nucleotide sequence-specificity is the basis for dsRNA's perceived biosafety. A biosafe approach to pollen beetle (Brassicogethes aeneus) management in oilseed rape (Brassica napus) agroecosystems is needed. We examined the potential for SIGS in B. aeneus, via bud feeding, a field-relevant dsRNA exposure route. Oilseed rape buds were uniformly treated with dsRNA designed to target αCOP in B. aeneus. Our model control dsRNA (dsGFP) remained detectable on buds throughout the entire 3 d exposure period. When applied at 5 µg/µL, dsαCOP induced significant αCOP silencing 3 d after dietary exposure to buds treated with this dsαCOP concentration. We also observed a trend of increased αCOP silencing with increasing concentrations of dsαCOP at both 3 and 6 d. Furthermore, we observed a marginally significant and significant reduction in B. aeneus survival at 10 and 15 d, respectively. Our results suggest potential for developing a SIGS approach to B. aeneus management-though further experiments are needed to more fully understand this potential.
DOI: 10.3390/insects11110769
PubMed: 33171797
Affiliations:
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Willow</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu</wicri:regionArea><wicri:noRegion>51006 Tartu</wicri:noRegion></affiliation></author><author><name sortKey="Soonvald, Liina" sort="Soonvald, Liina" uniqKey="Soonvald L" first="Liina" last="Soonvald">Liina Soonvald</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu</wicri:regionArea><wicri:noRegion>51006 Tartu</wicri:noRegion></affiliation></author><author><name sortKey="Sulg, Silva" sort="Sulg, Silva" uniqKey="Sulg S" first="Silva" last="Sulg">Silva Sulg</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu</wicri:regionArea><wicri:noRegion>51006 Tartu</wicri:noRegion></affiliation></author><author><name sortKey="Kaasik, Riina" sort="Kaasik, Riina" uniqKey="Kaasik R" first="Riina" last="Kaasik">Riina Kaasik</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu</wicri:regionArea><wicri:noRegion>51006 Tartu</wicri:noRegion></affiliation></author><author><name sortKey="Silva, Ana Isabel" sort="Silva, Ana Isabel" uniqKey="Silva A" first="Ana Isabel" last="Silva">Ana Isabel Silva</name><affiliation wicri:level="1"><nlm:affiliation>School of Mental Health and Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, 6229 ER Maastricht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>School of Mental Health and Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, 6229 ER Maastricht</wicri:regionArea><wicri:noRegion>6229 ER Maastricht</wicri:noRegion></affiliation></author><author><name sortKey="Taning, Clauvis Nji Tizi" sort="Taning, Clauvis Nji Tizi" uniqKey="Taning C" first="Clauvis Nji Tizi" last="Taning">Clauvis Nji Tizi Taning</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Christiaens, Olivier" sort="Christiaens, Olivier" uniqKey="Christiaens O" first="Olivier" last="Christiaens">Olivier Christiaens</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Smagghe, Guy" sort="Smagghe, Guy" uniqKey="Smagghe G" first="Guy" last="Smagghe">Guy Smagghe</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Veromann, Eve" sort="Veromann, Eve" uniqKey="Veromann E" first="Eve" last="Veromann">Eve Veromann</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu</wicri:regionArea><wicri:noRegion>51006 Tartu</wicri:noRegion></affiliation></author></analytic><series><title level="j">Insects</title><idno type="ISSN">2075-4450</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Spray-induced gene silencing (SIGS) is a potential strategy for agricultural pest management, whereby nucleotide sequence-specific double-stranded RNA (dsRNA) can be sprayed onto a crop; the desired effect being a consumption of dsRNA by the target pest, and subsequent gene silencing-induced mortality. Nucleotide sequence-specificity is the basis for dsRNA's perceived biosafety. A biosafe approach to pollen beetle (<i>Brassicogethes aeneus</i>) management in oilseed rape (<i>Brassica napus</i>) agroecosystems is needed. We examined the potential for SIGS in <i>B. aeneus</i>, via bud feeding, a field-relevant dsRNA exposure route. Oilseed rape buds were uniformly treated with dsRNA designed to target <i>αCOP</i> in <i>B. aeneus</i>. Our model control dsRNA (dsGFP) remained detectable on buds throughout the entire 3 d exposure period. When applied at 5 µg/µL, dsαCOP induced significant <i>αCOP</i> silencing 3 d after dietary exposure to buds treated with this dsαCOP concentration. We also observed a trend of increased <i>αCOP</i> silencing with increasing concentrations of dsαCOP at both 3 and 6 d. Furthermore, we observed a marginally significant and significant reduction in <i>B. aeneus</i> survival at 10 and 15 d, respectively. Our results suggest potential for developing a SIGS approach to <i>B. aeneus</i> management-though further experiments are needed to more fully understand this potential.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33171797</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>11</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2075-4450</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>07</Day></PubDate></JournalIssue><Title>Insects</Title><ISOAbbreviation>Insects</ISOAbbreviation></Journal><ArticleTitle>First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E769</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/insects11110769</ELocationID><Abstract><AbstractText>Spray-induced gene silencing (SIGS) is a potential strategy for agricultural pest management, whereby nucleotide sequence-specific double-stranded RNA (dsRNA) can be sprayed onto a crop; the desired effect being a consumption of dsRNA by the target pest, and subsequent gene silencing-induced mortality. Nucleotide sequence-specificity is the basis for dsRNA's perceived biosafety. A biosafe approach to pollen beetle (<i>Brassicogethes aeneus</i>) management in oilseed rape (<i>Brassica napus</i>) agroecosystems is needed. We examined the potential for SIGS in <i>B. aeneus</i>, via bud feeding, a field-relevant dsRNA exposure route. Oilseed rape buds were uniformly treated with dsRNA designed to target <i>αCOP</i> in <i>B. aeneus</i>. Our model control dsRNA (dsGFP) remained detectable on buds throughout the entire 3 d exposure period. When applied at 5 µg/µL, dsαCOP induced significant <i>αCOP</i> silencing 3 d after dietary exposure to buds treated with this dsαCOP concentration. We also observed a trend of increased <i>αCOP</i> silencing with increasing concentrations of dsαCOP at both 3 and 6 d. Furthermore, we observed a marginally significant and significant reduction in <i>B. aeneus</i> survival at 10 and 15 d, respectively. Our results suggest potential for developing a SIGS approach to <i>B. aeneus</i> management-though further experiments are needed to more fully understand this potential.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Willow</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0003-2377-4917</Identifier><AffiliationInfo><Affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soonvald</LastName><ForeName>Liina</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sulg</LastName><ForeName>Silva</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-4060-9596</Identifier><AffiliationInfo><Affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaasik</LastName><ForeName>Riina</ForeName><Initials>R</Initials><Identifier Source="ORCID">0000-0002-8090-2199</Identifier><AffiliationInfo><Affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Ana Isabel</ForeName><Initials>AI</Initials><Identifier Source="ORCID">0000-0002-1184-4909</Identifier><AffiliationInfo><Affiliation>School of Mental Health and Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, 6229 ER Maastricht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taning</LastName><ForeName>Clauvis Nji Tizi</ForeName><Initials>CNT</Initials><Identifier Source="ORCID">0000-0002-7597-1261</Identifier><AffiliationInfo><Affiliation>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Christiaens</LastName><ForeName>Olivier</ForeName><Initials>O</Initials><Identifier Source="ORCID">0000-0001-5850-340X</Identifier><AffiliationInfo><Affiliation>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smagghe</LastName><ForeName>Guy</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0001-8334-3313</Identifier><AffiliationInfo><Affiliation>Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Veromann</LastName><ForeName>Eve</ForeName><Initials>E</Initials><Identifier Source="ORCID">0000-0002-0977-5270</Identifier><AffiliationInfo><Affiliation>Chair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>IUT36-2</GrantID><Agency>Estonian Research Council</Agency><Country></Country></Grant><Grant><GrantID>3G0H0416</GrantID><Agency>ERA-NET C-IPM</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Insects</MedlineTA><NlmUniqueID>101574235</NlmUniqueID><ISSNLinking>2075-4450</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Coleoptera</Keyword><Keyword MajorTopicYN="N">Meligethes aeneus</Keyword><Keyword MajorTopicYN="N">Nitidulidae</Keyword><Keyword MajorTopicYN="N">RNA interference</Keyword><Keyword MajorTopicYN="N">biopesticide</Keyword><Keyword MajorTopicYN="N">insecticide</Keyword><Keyword MajorTopicYN="N">rapeseed</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate 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