Heterologous Expression of SvMBD5 from Salix viminalis L. Promotes Flowering in Arabidopsis thaliana L.
Identifieur interne : 000192 ( Main/Exploration ); précédent : 000191; suivant : 000193Heterologous Expression of SvMBD5 from Salix viminalis L. Promotes Flowering in Arabidopsis thaliana L.
Auteurs : Yunhe Cheng [République populaire de Chine] ; Lili Cheng [République populaire de Chine] ; Qingchang Cao [République populaire de Chine] ; Junzhu Zou [République populaire de Chine] ; Xia Li [République populaire de Chine] ; Xiaodong Ma [République populaire de Chine] ; Jingjing Zhou [République populaire de Chine] ; Feifei Zhai [République populaire de Chine] ; Zhenyuan Sun [République populaire de Chine] ; Yanping Lan [République populaire de Chine] ; Lei Han [République populaire de Chine]Source :
- Genes [ 2073-4425 ] ; 2020.
Abstract
Methyl-CpG-binding domain (MBD) proteins have diverse molecular and biological functions in plants. Most studies of MBD proteins in plants have focused on the model plant Arabidopsis thaliana L. Here we cloned SvMBD5 from the willow Salix viminalis L. by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed the structure of SvMBD5 and its evolutionary relationships with proteins in other species. The coding sequence of SvMBD5 is 645 bp long, encoding a 214 amino acid protein with a methyl-CpG-binding domain. SvMBD5 belongs to the same subfamily as AtMBD5 and AtMBD6 from Arabidopsis. Subcellular localization analysis showed that SvMBD5 is only expressed in the nucleus. We transformed Arabidopsis plants with a 35S::SvMBD5 expression construct to examine SvMBD5 function. The Arabidopsis SvMBD5-expressing line flowered earlier than the wild type. In the transgenic plants, the expression of FLOWERING LOCUS T and CONSTANS significantly increased, while the expression of FLOWERING LOCUS C greatly decreased. In addition, heterologously expressing SvMBD5 in Arabidopsis significantly inhibited the establishment and maintenance of methylation of CHROMOMETHYLASE 3 and METHYLTRANSFERASE 1, as well as their expression, and significantly increased the expression of the demethylation-related genes REPRESSOR OF SILENCING1 and DEMETER-LIKE PROTEIN3. Our findings suggest that SvMBD5 participates in the flowering process by regulating the methylation levels of flowering genes, laying the foundation for further studying the role of SvMBD5 in regulating DNA demethylation.
DOI: 10.3390/genes11030285
PubMed: 32156087
PubMed Central: PMC7140845
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Promotes Flowering in <i>Arabidopsis thaliana</i> L.</title><author><name sortKey="Cheng, Yunhe" sort="Cheng, Yunhe" uniqKey="Cheng Y" first="Yunhe" last="Cheng">Yunhe Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cheng, Lili" sort="Cheng, Lili" uniqKey="Cheng L" first="Lili" last="Cheng">Lili Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cao, Qingchang" sort="Cao, Qingchang" uniqKey="Cao Q" first="Qingchang" last="Cao">Qingchang Cao</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement 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Promotes Flowering in <i>Arabidopsis thaliana</i> L.</title><author><name sortKey="Cheng, Yunhe" sort="Cheng, Yunhe" uniqKey="Cheng Y" first="Yunhe" last="Cheng">Yunhe Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cheng, Lili" sort="Cheng, Lili" uniqKey="Cheng L" first="Lili" last="Cheng">Lili Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cao, Qingchang" sort="Cao, Qingchang" uniqKey="Cao Q" first="Qingchang" last="Cao">Qingchang Cao</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zou, Junzhu" sort="Zou, Junzhu" uniqKey="Zou J" first="Junzhu" last="Zou">Junzhu Zou</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Xia" sort="Li, Xia" uniqKey="Li X" first="Xia" last="Li">Xia Li</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture and Bioengineering, Heze University, Heze 274000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture and Bioengineering, Heze University, Heze 274000</wicri:regionArea><wicri:noRegion>Heze 274000</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Xiaodong" sort="Ma, Xiaodong" uniqKey="Ma X" first="Xiaodong" last="Ma">Xiaodong Ma</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhou, Jingjing" sort="Zhou, Jingjing" uniqKey="Zhou J" first="Jingjing" last="Zhou">Jingjing Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhai, Feifei" sort="Zhai, Feifei" uniqKey="Zhai F" first="Feifei" last="Zhai">Feifei Zhai</name><affiliation wicri:level="1"><nlm:affiliation>School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo 454000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo 454000</wicri:regionArea><wicri:noRegion>Jiaozuo 454000</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Zhenyuan" sort="Sun, Zhenyuan" uniqKey="Sun Z" first="Zhenyuan" last="Sun">Zhenyuan Sun</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lan, Yanping" sort="Lan, Yanping" uniqKey="Lan Y" first="Yanping" last="Lan">Yanping Lan</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Han, Lei" sort="Han, Lei" uniqKey="Han L" first="Lei" last="Han">Lei Han</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Genes</title><idno type="eISSN">2073-4425</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Methyl-CpG-binding domain (MBD) proteins have diverse molecular and biological functions in plants. Most studies of MBD proteins in plants have focused on the model plant <i>Arabidopsis thaliana</i> L. Here we cloned <i>SvMBD5</i> from the willow <i>Salix viminalis</i> L. by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed the structure of SvMBD5 and its evolutionary relationships with proteins in other species. The coding sequence of <i>SvMBD5</i> is 645 bp long, encoding a 214 amino acid protein with a methyl-CpG-binding domain. SvMBD5 belongs to the same subfamily as AtMBD5 and AtMBD6 from Arabidopsis. Subcellular localization analysis showed that <i>SvMBD5</i> is only expressed in the nucleus. We transformed Arabidopsis plants with a <i>35S::SvMBD5</i> expression construct to examine SvMBD5 function. The Arabidopsis <i>SvMBD5</i>-expressing line flowered earlier than the wild type. In the transgenic plants, the expression of <i>FLOWERING LOCUS T</i> and <i>CONSTANS</i> significantly increased, while the expression of <i>FLOWERING LOCUS C</i> greatly decreased. In addition, heterologously expressing <i>SvMBD5</i> in Arabidopsis significantly inhibited the establishment and maintenance of methylation of <i>CHROMOMETHYLASE 3</i> and <i>METHYLTRANSFERASE 1</i>, as well as their expression, and significantly increased the expression of the demethylation-related genes <i>REPRESSOR OF SILENCING1</i> and <i>DEMETER-LIKE PROTEIN3</i>. Our findings suggest that SvMBD5 participates in the flowering process by regulating the methylation levels of flowering genes, laying the foundation for further studying the role of SvMBD5 in regulating DNA demethylation.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32156087</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>23</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2073-4425</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>03</Month><Day>07</Day></PubDate></JournalIssue><Title>Genes</Title><ISOAbbreviation>Genes (Basel)</ISOAbbreviation></Journal><ArticleTitle>Heterologous Expression of <i>SvMBD5</i> from <i>Salix viminalis</i> L. Promotes Flowering in <i>Arabidopsis thaliana</i> L.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E285</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/genes11030285</ELocationID><Abstract><AbstractText>Methyl-CpG-binding domain (MBD) proteins have diverse molecular and biological functions in plants. Most studies of MBD proteins in plants have focused on the model plant <i>Arabidopsis thaliana</i> L. Here we cloned <i>SvMBD5</i> from the willow <i>Salix viminalis</i> L. by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed the structure of SvMBD5 and its evolutionary relationships with proteins in other species. The coding sequence of <i>SvMBD5</i> is 645 bp long, encoding a 214 amino acid protein with a methyl-CpG-binding domain. SvMBD5 belongs to the same subfamily as AtMBD5 and AtMBD6 from Arabidopsis. Subcellular localization analysis showed that <i>SvMBD5</i> is only expressed in the nucleus. We transformed Arabidopsis plants with a <i>35S::SvMBD5</i> expression construct to examine SvMBD5 function. The Arabidopsis <i>SvMBD5</i>-expressing line flowered earlier than the wild type. In the transgenic plants, the expression of <i>FLOWERING LOCUS T</i> and <i>CONSTANS</i> significantly increased, while the expression of <i>FLOWERING LOCUS C</i> greatly decreased. In addition, heterologously expressing <i>SvMBD5</i> in Arabidopsis significantly inhibited the establishment and maintenance of methylation of <i>CHROMOMETHYLASE 3</i> and <i>METHYLTRANSFERASE 1</i>, as well as their expression, and significantly increased the expression of the demethylation-related genes <i>REPRESSOR OF SILENCING1</i> and <i>DEMETER-LIKE PROTEIN3</i>. Our findings suggest that SvMBD5 participates in the flowering process by regulating the methylation levels of flowering genes, laying the foundation for further studying the role of SvMBD5 in regulating DNA demethylation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Yunhe</ForeName><Initials>Y</Initials><Identifier Source="ORCID">0000-0002-4008-6115</Identifier><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Lili</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Qingchang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zou</LastName><ForeName>Junzhu</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xia</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Agriculture and Bioengineering, Heze University, Heze 274000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Xiaodong</ForeName><Initials>X</Initials><Identifier Source="ORCID">0000-0002-6537-7920</Identifier><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Jingjing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhai</LastName><ForeName>Feifei</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo 454000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Zhenyuan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lan</LastName><ForeName>Yanping</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Lei</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Genes (Basel)</MedlineTA><NlmUniqueID>101551097</NlmUniqueID><ISSNLinking>2073-4425</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Salix viminalis</Keyword><Keyword MajorTopicYN="Y">SvMBD5</Keyword><Keyword MajorTopicYN="Y">demethylation</Keyword><Keyword MajorTopicYN="Y">flowering</Keyword><Keyword MajorTopicYN="Y">transgenic</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">23540698</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2007 Feb;12(2):80-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17208509</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2015 Nov;34(11):1857-62</Citation><ArticleIdList><ArticleId IdType="pubmed">26183954</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2015;66:243-67</Citation><ArticleIdList><ArticleId IdType="pubmed">25494460</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2019 Mar 30;690:90-98</Citation><ArticleIdList><ArticleId IdType="pubmed">30597235</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18729-34</Citation><ArticleIdList><ArticleId IdType="pubmed">20937895</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2006 Jun 21;25(12):2828-36</Citation><ArticleIdList><ArticleId IdType="pubmed">16724114</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Apr;46(2):282-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16623890</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2005 Oct;222(2):301-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15968513</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2011 Mar;21(3):442-65</Citation><ArticleIdList><ArticleId IdType="pubmed">21321601</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2010 Mar;11(3):204-20</Citation><ArticleIdList><ArticleId IdType="pubmed">20142834</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1998 Dec;16(6):735-43</Citation><ArticleIdList><ArticleId IdType="pubmed">10069079</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Mol Cell Biol. 2018 Aug;19(8):489-506</Citation><ArticleIdList><ArticleId IdType="pubmed">29784956</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2018 Mar 26;19(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">29587418</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2007 May-Jun;1769(5-6):287-94</Citation><ArticleIdList><ArticleId IdType="pubmed">17407793</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2007;2(10):2348-53</Citation><ArticleIdList><ArticleId IdType="pubmed">17947977</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2005 Nov;44(3):420-32</Citation><ArticleIdList><ArticleId IdType="pubmed">16236152</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2017 May 31;18(1):99</Citation><ArticleIdList><ArticleId IdType="pubmed">28558752</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 Jun;34(5):565-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12787239</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2009 Jul;59(1):123-35</Citation><ArticleIdList><ArticleId IdType="pubmed">19419532</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2014 Jan;21(1):64-72</Citation><ArticleIdList><ArticleId IdType="pubmed">24336224</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2002 Dec 13;111(6):803-14</Citation><ArticleIdList><ArticleId IdType="pubmed">12526807</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 May 11;107(19):8689-94</Citation><ArticleIdList><ArticleId IdType="pubmed">20395551</ArticleId></ArticleIdList></Reference><Reference><Citation>Physiol Plant. 2009 May;136(1):110-26</Citation><ArticleIdList><ArticleId IdType="pubmed">19374717</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W369-73</Citation><ArticleIdList><ArticleId IdType="pubmed">16845028</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Feb 10;124(3):495-506</Citation><ArticleIdList><ArticleId IdType="pubmed">16469697</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2016 Feb 1;9(2):305-307</Citation><ArticleIdList><ArticleId IdType="pubmed">26455460</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2003 Mar;163(3):1109-22</Citation><ArticleIdList><ArticleId IdType="pubmed">12663548</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2013 Jan;64(2):405-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23125359</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2007 Jan;39(1):61-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17128275</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2007 Jan 9;17(1):54-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17208187</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2003 Sep 15;31(18):5291-304</Citation><ArticleIdList><ArticleId IdType="pubmed">12954765</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2003 Dec;133(4):1747-54</Citation><ArticleIdList><ArticleId IdType="pubmed">14605234</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2014 Mar 6;507(7490):124-128</Citation><ArticleIdList><ArticleId IdType="pubmed">24463519</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2015 Jan;81(2):268-81</Citation><ArticleIdList><ArticleId IdType="pubmed">25404462</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2001 Jun 15;292(5524):2077-80</Citation><ArticleIdList><ArticleId IdType="pubmed">11349138</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 May 2;103(18):6853-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16624880</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Sep 22;126(6):1189-201</Citation><ArticleIdList><ArticleId IdType="pubmed">16949657</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1993 Jun 25;260(5116):1926-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8316832</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2005 May;138(1):92-104</Citation><ArticleIdList><ArticleId IdType="pubmed">15888682</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11796-801</Citation><ArticleIdList><ArticleId IdType="pubmed">16864782</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8334-9</Citation><ArticleIdList><ArticleId IdType="pubmed">8710871</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2012 May 25;336(6084):1045-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22628657</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biosci. 2017 Mar;42(1):57-68</Citation><ArticleIdList><ArticleId IdType="pubmed">28229965</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Plants. 2016 Oct 31;2(11):16169</Citation><ArticleIdList><ArticleId IdType="pubmed">27797352</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2008 Aug;67(6):671-81</Citation><ArticleIdList><ArticleId IdType="pubmed">18493721</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Jan 17;13:27</Citation><ArticleIdList><ArticleId IdType="pubmed">22251412</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Cheng, Yunhe" sort="Cheng, Yunhe" uniqKey="Cheng Y" first="Yunhe" 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