NTZIP antisense plants show reduced chlorophyll levels.
Identifieur interne : 001E05 ( Main/Corpus ); précédent : 001E04; suivant : 001E06NTZIP antisense plants show reduced chlorophyll levels.
Auteurs : Ning Liu ; Yu-Tao Yang ; Han-Hua Liu ; Guo-Dong Yang ; Nai-Hua Zhang ; Cheng Chao ZhengSource :
- Plant physiology and biochemistry : PPB [ 0981-9428 ] ; 2004.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Blotting, Northern (MeSH), Blotting, Southern (MeSH), Carbon Dioxide (metabolism), Chlorophyll (biosynthesis), DNA, Plant (analysis), Genes, Plant (MeSH), Leucine Zippers (genetics), Molecular Sequence Data (MeSH), Photosynthesis (physiology), Plant Leaves (genetics), Plant Leaves (physiology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Protein Structure, Secondary (MeSH), RNA, Antisense (genetics), RNA, Messenger (biosynthesis), RNA, Plant (genetics), Tobacco (genetics), Tobacco (metabolism).
- MESH :
- chemical , analysis : DNA, Plant.
- chemical , biosynthesis : Chlorophyll, RNA, Messenger.
- chemical , genetics : RNA, Antisense, RNA, Plant.
- chemical , metabolism : Carbon Dioxide.
- genetics : Leucine Zippers, Plant Leaves, Plants, Genetically Modified, Tobacco.
- metabolism : Plants, Genetically Modified, Tobacco.
- physiology : Photosynthesis, Plant Leaves.
- Amino Acid Sequence, Blotting, Northern, Blotting, Southern, Genes, Plant, Molecular Sequence Data, Protein Structure, Secondary.
Abstract
We have isolated and characterized a new photosynthetic tissue-specific gene NTZIP (Nicotiana tabacum leucine zipper) from tobacco (N. tabacum). Its deduced amino acid sequence has two highly conserved regions, leucine zipper and [EX(n)DEXRH](2) motifs, which are related to the gene's biochemical functions. NTZIP was expressed in leaves and stems, but was not detected in roots or flowers, suggesting that its physiological functions might be associated with photosynthesis. Northern blot analysis showed that NTZIP mRNA accumulation was induced by light signals, increased greatly under low temperatures and was repressed by strong light illumination. Furthermore, a number of homologs of NTZIP were isolated from cucumber (Cucumis sativus), rape (Brassica napus), clover (Trifolium repens), willow (Salix babylonica), rosebush (Rusa dovurica), wheat (Triticum aestivum) and spinach (Spinacia oleracea), proving the ubiquitous existence of the NTZIP-like genes in higher plants. Transgenic tobaccos constitutively expressing antisense RNA to NTZIP displayed chlorosis and a lack of ability to turn green even under normal growth conditions. The chlorophyll deficiency was further confirmed by chlorophyll content determination and gas exchange analysis. Based on these observations, we propose that NTZIP may be involved in chlorophyll biosynthesis, and might define a novel family of evolutionarily conserved proteins with its homologs in other plant species.
DOI: 10.1016/j.plaphy.2004.02.007
PubMed: 15120117
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pubmed:15120117Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">NTZIP antisense plants show reduced chlorophyll levels.</title><author><name sortKey="Liu, Ning" sort="Liu, Ning" uniqKey="Liu N" first="Ning" last="Liu">Ning Liu</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Yu Tao" sort="Yang, Yu Tao" uniqKey="Yang Y" first="Yu-Tao" last="Yang">Yu-Tao Yang</name></author><author><name sortKey="Liu, Han Hua" sort="Liu, Han Hua" uniqKey="Liu H" first="Han-Hua" last="Liu">Han-Hua Liu</name></author><author><name sortKey="Yang, Guo Dong" sort="Yang, Guo Dong" uniqKey="Yang G" first="Guo-Dong" last="Yang">Guo-Dong Yang</name></author><author><name sortKey="Zhang, Nai Hua" sort="Zhang, Nai Hua" uniqKey="Zhang N" first="Nai-Hua" last="Zhang">Nai-Hua Zhang</name></author><author><name sortKey="Zheng, Cheng Chao" sort="Zheng, Cheng Chao" uniqKey="Zheng C" first="Cheng Chao" last="Zheng">Cheng Chao Zheng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15120117</idno><idno type="pmid">15120117</idno><idno type="doi">10.1016/j.plaphy.2004.02.007</idno><idno type="wicri:Area/Main/Corpus">001E05</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001E05</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">NTZIP antisense plants show reduced chlorophyll levels.</title><author><name sortKey="Liu, Ning" sort="Liu, Ning" uniqKey="Liu N" first="Ning" last="Liu">Ning Liu</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Yu Tao" sort="Yang, Yu Tao" uniqKey="Yang Y" first="Yu-Tao" last="Yang">Yu-Tao Yang</name></author><author><name sortKey="Liu, Han Hua" sort="Liu, Han Hua" uniqKey="Liu H" first="Han-Hua" last="Liu">Han-Hua Liu</name></author><author><name sortKey="Yang, Guo Dong" sort="Yang, Guo Dong" uniqKey="Yang G" first="Guo-Dong" last="Yang">Guo-Dong Yang</name></author><author><name sortKey="Zhang, Nai Hua" sort="Zhang, Nai Hua" uniqKey="Zhang N" first="Nai-Hua" last="Zhang">Nai-Hua Zhang</name></author><author><name sortKey="Zheng, Cheng Chao" sort="Zheng, Cheng Chao" uniqKey="Zheng C" first="Cheng Chao" last="Zheng">Cheng Chao Zheng</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="ISSN">0981-9428</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Blotting, Northern (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Carbon Dioxide (metabolism)</term><term>Chlorophyll (biosynthesis)</term><term>DNA, Plant (analysis)</term><term>Genes, Plant (MeSH)</term><term>Leucine Zippers (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Photosynthesis (physiology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (physiology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Protein Structure, Secondary (MeSH)</term><term>RNA, Antisense (genetics)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Plant (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Chlorophyll</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Antisense</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon Dioxide</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Leucine Zippers</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Photosynthesis</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Blotting, Northern</term><term>Blotting, Southern</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have isolated and characterized a new photosynthetic tissue-specific gene NTZIP (Nicotiana tabacum leucine zipper) from tobacco (N. tabacum). Its deduced amino acid sequence has two highly conserved regions, leucine zipper and [EX(n)DEXRH](2) motifs, which are related to the gene's biochemical functions. NTZIP was expressed in leaves and stems, but was not detected in roots or flowers, suggesting that its physiological functions might be associated with photosynthesis. Northern blot analysis showed that NTZIP mRNA accumulation was induced by light signals, increased greatly under low temperatures and was repressed by strong light illumination. Furthermore, a number of homologs of NTZIP were isolated from cucumber (Cucumis sativus), rape (Brassica napus), clover (Trifolium repens), willow (Salix babylonica), rosebush (Rusa dovurica), wheat (Triticum aestivum) and spinach (Spinacia oleracea), proving the ubiquitous existence of the NTZIP-like genes in higher plants. Transgenic tobaccos constitutively expressing antisense RNA to NTZIP displayed chlorosis and a lack of ability to turn green even under normal growth conditions. The chlorophyll deficiency was further confirmed by chlorophyll content determination and gas exchange analysis. Based on these observations, we propose that NTZIP may be involved in chlorophyll biosynthesis, and might define a novel family of evolutionarily conserved proteins with its homologs in other plant species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15120117</PMID><DateCompleted><Year>2004</Year><Month>07</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0981-9428</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>4</Issue><PubDate><Year>2004</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>NTZIP antisense plants show reduced chlorophyll levels.</ArticleTitle><Pagination><MedlinePgn>321-7</MedlinePgn></Pagination><Abstract><AbstractText>We have isolated and characterized a new photosynthetic tissue-specific gene NTZIP (Nicotiana tabacum leucine zipper) from tobacco (N. tabacum). Its deduced amino acid sequence has two highly conserved regions, leucine zipper and [EX(n)DEXRH](2) motifs, which are related to the gene's biochemical functions. NTZIP was expressed in leaves and stems, but was not detected in roots or flowers, suggesting that its physiological functions might be associated with photosynthesis. Northern blot analysis showed that NTZIP mRNA accumulation was induced by light signals, increased greatly under low temperatures and was repressed by strong light illumination. Furthermore, a number of homologs of NTZIP were isolated from cucumber (Cucumis sativus), rape (Brassica napus), clover (Trifolium repens), willow (Salix babylonica), rosebush (Rusa dovurica), wheat (Triticum aestivum) and spinach (Spinacia oleracea), proving the ubiquitous existence of the NTZIP-like genes in higher plants. Transgenic tobaccos constitutively expressing antisense RNA to NTZIP displayed chlorosis and a lack of ability to turn green even under normal growth conditions. The chlorophyll deficiency was further confirmed by chlorophyll content determination and gas exchange analysis. Based on these observations, we propose that NTZIP may be involved in chlorophyll biosynthesis, and might define a novel family of evolutionarily conserved proteins with its homologs in other plant species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ning</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yu-Tao</ForeName><Initials>YT</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Han-Hua</ForeName><Initials>HH</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Guo-Dong</ForeName><Initials>GD</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Nai-Hua</ForeName><Initials>NH</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Cheng Chao</ForeName><Initials>CC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016372">RNA, Antisense</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>142M471B3J</RegistryNumber><NameOfSubstance UI="D002245">Carbon Dioxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002245" MajorTopicYN="N">Carbon Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000096" 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MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017433" MajorTopicYN="N">Protein Structure, Secondary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016372" MajorTopicYN="N">RNA, Antisense</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" 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