Thin-layer chromatography and multivariate data analysis of willow bark extracts.
Identifieur interne : 001D84 ( Main/Corpus ); précédent : 001D83; suivant : 001D85Thin-layer chromatography and multivariate data analysis of willow bark extracts.
Auteurs : Kim Wuthold ; Ines Germann ; Gudrun Roos ; Olaf Kelber ; D. Weiser ; Helmut Heinle ; Karl-Artur KovarSource :
- Journal of chromatographic science [ 0021-9665 ] ; 2004.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Plant Extracts.
- chemistry : Plant Bark, Salix.
- methods : Chromatography, Thin Layer.
- chemical , pharmacology : Plant Extracts.
- Multivariate Analysis.
Abstract
In most cases the pharmacological activity of plant extracts is not assigned to single components and often not all active ingredients are known. Approaches other than those considering single compounds only to analyze plant material have proven helpful for a better characterization of extracts in their entirety. In this study extracts of willow bark are analyzed by high-performance thin-layer chromatography (HPTLC) and two different pharmacological tests [the 2,2'-azobis (2-amidinopropane) dihydrochloride reaction and the xanthine/xanthine oxidase reaction] with the help of multivariate data analysis. Described are two models using the results of the chromatographic study of 22 various extracts of willow bark and their pharmacological properties. The chromatographic data are obtained by a special TLC scanner that enables measurement of HPTLC tracks simultaneously in the range of lambda = 200-400 nm. Additionally, the developed models are used to predict the activity of another three extracts of willow bark demonstrating the quality of the model.
DOI: 10.1093/chromsci/42.6.306
PubMed: 15296530
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pubmed:15296530Le document en format XML
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Weiser</name></author><author><name sortKey="Heinle, Helmut" sort="Heinle, Helmut" uniqKey="Heinle H" first="Helmut" last="Heinle">Helmut Heinle</name></author><author><name sortKey="Kovar, Karl Artur" sort="Kovar, Karl Artur" uniqKey="Kovar K" first="Karl-Artur" last="Kovar">Karl-Artur Kovar</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15296530</idno><idno type="pmid">15296530</idno><idno type="doi">10.1093/chromsci/42.6.306</idno><idno type="wicri:Area/Main/Corpus">001D84</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001D84</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Thin-layer chromatography and multivariate data analysis of willow bark extracts.</title><author><name sortKey="Wuthold, Kim" sort="Wuthold, Kim" uniqKey="Wuthold K" first="Kim" last="Wuthold">Kim Wuthold</name><affiliation><nlm:affiliation>Department of Pharmacy, Eberhard-Karls-University, Auf der Morgenstelle 8, 64295 Darmstadt, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Germann, Ines" sort="Germann, Ines" uniqKey="Germann I" first="Ines" last="Germann">Ines Germann</name></author><author><name sortKey="Roos, Gudrun" sort="Roos, Gudrun" uniqKey="Roos G" first="Gudrun" last="Roos">Gudrun Roos</name></author><author><name sortKey="Kelber, Olaf" sort="Kelber, Olaf" uniqKey="Kelber O" first="Olaf" last="Kelber">Olaf Kelber</name></author><author><name sortKey="Weiser, D" sort="Weiser, D" uniqKey="Weiser D" first="D" last="Weiser">D. Weiser</name></author><author><name sortKey="Heinle, Helmut" sort="Heinle, Helmut" uniqKey="Heinle H" first="Helmut" last="Heinle">Helmut Heinle</name></author><author><name sortKey="Kovar, Karl Artur" sort="Kovar, Karl Artur" uniqKey="Kovar K" first="Karl-Artur" last="Kovar">Karl-Artur Kovar</name></author></analytic><series><title level="j">Journal of chromatographic science</title><idno type="ISSN">0021-9665</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, Thin Layer (methods)</term><term>Multivariate Analysis (MeSH)</term><term>Plant Bark (chemistry)</term><term>Plant Extracts (analysis)</term><term>Plant Extracts (pharmacology)</term><term>Salix (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Bark</term><term>Salix</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Thin Layer</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Multivariate Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In most cases the pharmacological activity of plant extracts is not assigned to single components and often not all active ingredients are known. Approaches other than those considering single compounds only to analyze plant material have proven helpful for a better characterization of extracts in their entirety. In this study extracts of willow bark are analyzed by high-performance thin-layer chromatography (HPTLC) and two different pharmacological tests [the 2,2'-azobis (2-amidinopropane) dihydrochloride reaction and the xanthine/xanthine oxidase reaction] with the help of multivariate data analysis. Described are two models using the results of the chromatographic study of 22 various extracts of willow bark and their pharmacological properties. The chromatographic data are obtained by a special TLC scanner that enables measurement of HPTLC tracks simultaneously in the range of lambda = 200-400 nm. Additionally, the developed models are used to predict the activity of another three extracts of willow bark demonstrating the quality of the model.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15296530</PMID><DateCompleted><Year>2004</Year><Month>11</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9665</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>6</Issue><PubDate><Year>2004</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Journal of chromatographic science</Title><ISOAbbreviation>J Chromatogr Sci</ISOAbbreviation></Journal><ArticleTitle>Thin-layer chromatography and multivariate data analysis of willow bark extracts.</ArticleTitle><Pagination><MedlinePgn>306-9</MedlinePgn></Pagination><Abstract><AbstractText>In most cases the pharmacological activity of plant extracts is not assigned to single components and often not all active ingredients are known. Approaches other than those considering single compounds only to analyze plant material have proven helpful for a better characterization of extracts in their entirety. In this study extracts of willow bark are analyzed by high-performance thin-layer chromatography (HPTLC) and two different pharmacological tests [the 2,2'-azobis (2-amidinopropane) dihydrochloride reaction and the xanthine/xanthine oxidase reaction] with the help of multivariate data analysis. Described are two models using the results of the chromatographic study of 22 various extracts of willow bark and their pharmacological properties. The chromatographic data are obtained by a special TLC scanner that enables measurement of HPTLC tracks simultaneously in the range of lambda = 200-400 nm. Additionally, the developed models are used to predict the activity of another three extracts of willow bark demonstrating the quality of the model.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wuthold</LastName><ForeName>Kim</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Eberhard-Karls-University, Auf der Morgenstelle 8, 64295 Darmstadt, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Germann</LastName><ForeName>Ines</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Roos</LastName><ForeName>Gudrun</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Kelber</LastName><ForeName>Olaf</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Weiser</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Heinle</LastName><ForeName>Helmut</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Kovar</LastName><ForeName>Karl-Artur</ForeName><Initials>KA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Chromatogr Sci</MedlineTA><NlmUniqueID>0173225</NlmUniqueID><ISSNLinking>0021-9665</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002855" MajorTopicYN="N">Chromatography, Thin Layer</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015999" MajorTopicYN="N">Multivariate Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D024301" MajorTopicYN="N">Plant Bark</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>8</Month><Day>7</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>11</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>8</Month><Day>7</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15296530</ArticleId><ArticleId IdType="doi">10.1093/chromsci/42.6.306</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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