Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).
Identifieur interne : 000803 ( Main/Corpus ); précédent : 000802; suivant : 000804Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).
Auteurs : Alex J. Krotulski ; Amanda L A. Mohr ; Melissa Friscia ; Barry K. LoganSource :
- Journal of analytical toxicology [ 1945-2403 ] ; 2018.
English descriptors
- KwdEn :
- Chromatography, Liquid (MeSH), Equipment Design (MeSH), Florida (MeSH), Humans (MeSH), Illicit Drugs (analysis), Limit of Detection (MeSH), Point-of-Care Systems (MeSH), Point-of-Care Testing (MeSH), Predictive Value of Tests (MeSH), Reproducibility of Results (MeSH), Saliva (chemistry), Substance Abuse Detection (instrumentation), Substance Abuse Detection (methods), Substance-Related Disorders (diagnosis), Substance-Related Disorders (metabolism), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , analysis : Illicit Drugs.
- geographic : Florida.
- chemistry : Saliva.
- diagnosis : Substance-Related Disorders.
- instrumentation : Substance Abuse Detection.
- metabolism : Substance-Related Disorders.
- methods : Substance Abuse Detection.
- Chromatography, Liquid, Equipment Design, Humans, Limit of Detection, Point-of-Care Systems, Point-of-Care Testing, Predictive Value of Tests, Reproducibility of Results, Tandem Mass Spectrometry.
Abstract
The collection and analysis of drugs in oral fluid (OF) at the roadside has become more feasible with the introduction of portable testing devices such as the Alere™ DDS®2 Mobile Test System (DDS®2). The objective of this study was to compare the on-site results for the DDS®2 to laboratory-based confirmatory assays with respect to detection of drugs of abuse in human subjects. As part of a larger Institutional Review Board approved study, two OF samples were collected from each participant at a music festival in Miami, FL, USA. One OF sample was field screened using the DDS®2, and a confirmatory OF sample was collected using the Quantisal™ OF collection device and submitted to the laboratory for testing. In total, 124 subjects participated in this study providing two contemporaneous OF samples. DDS®2 field screening yielded positive results for delta-9-tetrahydrocannabinol (THC) (n = 27), cocaine (n = 12), amphetamine (n = 3), methamphetamine (n = 3) and benzodiazepine (n = 1). No opiate-positive OF samples were detected. For cocaine, amphetamine, methamphetamine and benzodiazepines, the DDS®2 displayed sensitivity, specificity and accuracy of 100%. For THC, the DDS®2 displayed sensitivity of 90%, specificity of 100% and accuracy of 97.5%, when the threshold for confirmation matched that of the manufacturers advertised cut-off. When this confirmatory threshold was lowered to the analytical limit of detection (i.e., 1 ng/mL), apparent device performance for THC was poorer due to additional samples testing positive by confirmatory assay that had tested negative on the DDS®2, demonstrating a need for correlation between manufacturer cut-off and analytical reporting limit. These results from drug-using subjects demonstrate the value of field-based OF testing, and illustrate the significance of selecting an appropriate confirmation cut-off concentration with respect to performance evaluation and detection of drug use.
DOI: 10.1093/jat/bkx105
PubMed: 29301050
Links to Exploration step
pubmed:29301050Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).</title><author><name sortKey="Krotulski, Alex J" sort="Krotulski, Alex J" uniqKey="Krotulski A" first="Alex J" last="Krotulski">Alex J. Krotulski</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Mohr, Amanda L A" sort="Mohr, Amanda L A" uniqKey="Mohr A" first="Amanda L A" last="Mohr">Amanda L A. Mohr</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Friscia, Melissa" sort="Friscia, Melissa" uniqKey="Friscia M" first="Melissa" last="Friscia">Melissa Friscia</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Logan, Barry K" sort="Logan, Barry K" uniqKey="Logan B" first="Barry K" last="Logan">Barry K. Logan</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>NMS Labs, 3701 Welsh Rd, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29301050</idno><idno type="pmid">29301050</idno><idno type="doi">10.1093/jat/bkx105</idno><idno type="wicri:Area/Main/Corpus">000803</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000803</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).</title><author><name sortKey="Krotulski, Alex J" sort="Krotulski, Alex J" uniqKey="Krotulski A" first="Alex J" last="Krotulski">Alex J. Krotulski</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Mohr, Amanda L A" sort="Mohr, Amanda L A" uniqKey="Mohr A" first="Amanda L A" last="Mohr">Amanda L A. Mohr</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Friscia, Melissa" sort="Friscia, Melissa" uniqKey="Friscia M" first="Melissa" last="Friscia">Melissa Friscia</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Logan, Barry K" sort="Logan, Barry K" uniqKey="Logan B" first="Barry K" last="Logan">Barry K. Logan</name><affiliation><nlm:affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>NMS Labs, 3701 Welsh Rd, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of analytical toxicology</title><idno type="eISSN">1945-2403</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, Liquid (MeSH)</term><term>Equipment Design (MeSH)</term><term>Florida (MeSH)</term><term>Humans (MeSH)</term><term>Illicit Drugs (analysis)</term><term>Limit of Detection (MeSH)</term><term>Point-of-Care Systems (MeSH)</term><term>Point-of-Care Testing (MeSH)</term><term>Predictive Value of Tests (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Saliva (chemistry)</term><term>Substance Abuse Detection (instrumentation)</term><term>Substance Abuse Detection (methods)</term><term>Substance-Related Disorders (diagnosis)</term><term>Substance-Related Disorders (metabolism)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Illicit Drugs</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Florida</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Saliva</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Substance-Related Disorders</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Substance Abuse Detection</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Substance-Related Disorders</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Substance Abuse Detection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Liquid</term><term>Equipment Design</term><term>Humans</term><term>Limit of Detection</term><term>Point-of-Care Systems</term><term>Point-of-Care Testing</term><term>Predictive Value of Tests</term><term>Reproducibility of Results</term><term>Tandem Mass Spectrometry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The collection and analysis of drugs in oral fluid (OF) at the roadside has become more feasible with the introduction of portable testing devices such as the Alere™ DDS®2 Mobile Test System (DDS®2). The objective of this study was to compare the on-site results for the DDS®2 to laboratory-based confirmatory assays with respect to detection of drugs of abuse in human subjects. As part of a larger Institutional Review Board approved study, two OF samples were collected from each participant at a music festival in Miami, FL, USA. One OF sample was field screened using the DDS®2, and a confirmatory OF sample was collected using the Quantisal™ OF collection device and submitted to the laboratory for testing. In total, 124 subjects participated in this study providing two contemporaneous OF samples. DDS®2 field screening yielded positive results for delta-9-tetrahydrocannabinol (THC) (n = 27), cocaine (n = 12), amphetamine (n = 3), methamphetamine (n = 3) and benzodiazepine (n = 1). No opiate-positive OF samples were detected. For cocaine, amphetamine, methamphetamine and benzodiazepines, the DDS®2 displayed sensitivity, specificity and accuracy of 100%. For THC, the DDS®2 displayed sensitivity of 90%, specificity of 100% and accuracy of 97.5%, when the threshold for confirmation matched that of the manufacturers advertised cut-off. When this confirmatory threshold was lowered to the analytical limit of detection (i.e., 1 ng/mL), apparent device performance for THC was poorer due to additional samples testing positive by confirmatory assay that had tested negative on the DDS®2, demonstrating a need for correlation between manufacturer cut-off and analytical reporting limit. These results from drug-using subjects demonstrate the value of field-based OF testing, and illustrate the significance of selecting an appropriate confirmation cut-off concentration with respect to performance evaluation and detection of drug use.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29301050</PMID><DateCompleted><Year>2018</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1945-2403</ISSN><JournalIssue CitedMedium="Internet"><Volume>42</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>Apr</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of analytical toxicology</Title><ISOAbbreviation>J Anal Toxicol</ISOAbbreviation></Journal><ArticleTitle>Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).</ArticleTitle><Pagination><MedlinePgn>170-176</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jat/bkx105</ELocationID><Abstract><AbstractText>The collection and analysis of drugs in oral fluid (OF) at the roadside has become more feasible with the introduction of portable testing devices such as the Alere™ DDS®2 Mobile Test System (DDS®2). The objective of this study was to compare the on-site results for the DDS®2 to laboratory-based confirmatory assays with respect to detection of drugs of abuse in human subjects. As part of a larger Institutional Review Board approved study, two OF samples were collected from each participant at a music festival in Miami, FL, USA. One OF sample was field screened using the DDS®2, and a confirmatory OF sample was collected using the Quantisal™ OF collection device and submitted to the laboratory for testing. In total, 124 subjects participated in this study providing two contemporaneous OF samples. DDS®2 field screening yielded positive results for delta-9-tetrahydrocannabinol (THC) (n = 27), cocaine (n = 12), amphetamine (n = 3), methamphetamine (n = 3) and benzodiazepine (n = 1). No opiate-positive OF samples were detected. For cocaine, amphetamine, methamphetamine and benzodiazepines, the DDS®2 displayed sensitivity, specificity and accuracy of 100%. For THC, the DDS®2 displayed sensitivity of 90%, specificity of 100% and accuracy of 97.5%, when the threshold for confirmation matched that of the manufacturers advertised cut-off. When this confirmatory threshold was lowered to the analytical limit of detection (i.e., 1 ng/mL), apparent device performance for THC was poorer due to additional samples testing positive by confirmatory assay that had tested negative on the DDS®2, demonstrating a need for correlation between manufacturer cut-off and analytical reporting limit. These results from drug-using subjects demonstrate the value of field-based OF testing, and illustrate the significance of selecting an appropriate confirmation cut-off concentration with respect to performance evaluation and detection of drug use.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Krotulski</LastName><ForeName>Alex J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mohr</LastName><ForeName>Amanda L A</ForeName><Initials>ALA</Initials><AffiliationInfo><Affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Friscia</LastName><ForeName>Melissa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Logan</LastName><ForeName>Barry K</ForeName><Initials>BK</Initials><AffiliationInfo><Affiliation>Center for Forensic Science Research and Education at the Fredric Rieders Family Foundation, 2300 Stratford Ave, Willow Grove, PA 19090, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>NMS Labs, 3701 Welsh Rd, Willow Grove, PA 19090, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Anal Toxicol</MedlineTA><NlmUniqueID>7705085</NlmUniqueID><ISSNLinking>0146-4760</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013287">Illicit Drugs</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002853" MajorTopicYN="Y">Chromatography, Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004867" MajorTopicYN="N">Equipment Design</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005431" MajorTopicYN="N" Type="Geographic">Florida</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013287" MajorTopicYN="N">Illicit Drugs</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019095" MajorTopicYN="N">Point-of-Care Systems</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000067716" MajorTopicYN="Y">Point-of-Care Testing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011237" MajorTopicYN="N">Predictive Value of Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012463" MajorTopicYN="N">Saliva</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015813" MajorTopicYN="N">Substance Abuse Detection</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019966" MajorTopicYN="N">Substance-Related Disorders</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="Y">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>09</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29301050</ArticleId><ArticleId IdType="pii">4780787</ArticleId><ArticleId IdType="doi">10.1093/jat/bkx105</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/WillowV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000803 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000803 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= WillowV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:29301050
|texte= Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:29301050" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a WillowV1
| This area was generated with Dilib version V0.6.37. |  |