Quantitation of peptides from non-invasive skin tapings using isotope dilution and tandem mass spectrometry.
Identifieur interne : 000745 ( Main/Corpus ); précédent : 000744; suivant : 000746Quantitation of peptides from non-invasive skin tapings using isotope dilution and tandem mass spectrometry.
Auteurs : Nichole Reisdorph ; Michael Armstrong ; Roger Powell ; Kevin Quinn ; Kevin Legg ; Donald Leung ; Rick ReisdorphSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1873-376X ] ; 2018.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Peptides.
- chemistry : Skin.
- metabolism : Dermatitis, Atopic.
- methods : Isotope Labeling, Proteomics, Tandem Mass Spectrometry.
- Case-Control Studies, Humans, Limit of Detection, Linear Models, Reproducibility of Results.
Abstract
Previous work from our laboratories utilized a novel skin taping method and mass spectrometry-based proteomics to discover clinical biomarkers of skin conditions; these included atopic dermatitis, Staphylococcus aureus colonization, and eczema herpeticum. While suitable for discovery purposes, semi-quantitative proteomics is generally time-consuming and expensive. Furthermore, depending on the method used, discovery-based proteomics can result in high variation and inadequate sensitivity to detect low abundant peptides. Therefore, we strove to develop a rapid, sensitive, and reproducible method to quantitate disease-related proteins from skin tapings. We utilized isotopically-labeled peptides and tandem mass spectrometry to obtain absolute quantitation values on 14 peptides from 7 proteins; these proteins had shown previous importance in skin disease. The method demonstrated good reproducibility, dynamic range, and linearity (R2 > 0.993) when n = 3 standards were analyzed across 0.05-2.5 pmol. The method was used to determine if differences exist between skin proteins in a small group of atopic versus non-atopic individuals (n = 12). While only minimal differences were found, peptides were detected in all samples and exhibited good correlation between peptides for 5 of the 7 proteins (R2 = 0.71-0.98). This method can be applied to larger cohorts to further establish the relationships of these proteins to skin disease.
DOI: 10.1016/j.jchromb.2018.03.031
PubMed: 29601982
PubMed Central: PMC6093185
Links to Exploration step
pubmed:29601982Le document en format XML
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Electronic address: Nichole.Reisdorph@UCDenver.edu.</nlm:affiliation></affiliation></author><author><name sortKey="Armstrong, Michael" sort="Armstrong, Michael" uniqKey="Armstrong M" first="Michael" last="Armstrong">Michael Armstrong</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Powell, Roger" sort="Powell, Roger" uniqKey="Powell R" first="Roger" last="Powell">Roger Powell</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Quinn, Kevin" sort="Quinn, Kevin" uniqKey="Quinn K" first="Kevin" last="Quinn">Kevin Quinn</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Legg, Kevin" sort="Legg, Kevin" uniqKey="Legg K" first="Kevin" last="Legg">Kevin Legg</name><affiliation><nlm:affiliation>The Center for Forensic Science Research and Education, 2300 Stratford Avenue, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Leung, Donald" sort="Leung, Donald" uniqKey="Leung D" first="Donald" last="Leung">Donald Leung</name><affiliation><nlm:affiliation>Department of Pediatrics, National Jewish Health, Denver, CO 80206, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Reisdorph, Rick" sort="Reisdorph, Rick" uniqKey="Reisdorph R" first="Rick" last="Reisdorph">Rick Reisdorph</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29601982</idno><idno type="pmid">29601982</idno><idno type="doi">10.1016/j.jchromb.2018.03.031</idno><idno type="pmc">PMC6093185</idno><idno type="wicri:Area/Main/Corpus">000745</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000745</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantitation of peptides from non-invasive skin tapings using isotope dilution and tandem mass spectrometry.</title><author><name sortKey="Reisdorph, Nichole" sort="Reisdorph, Nichole" uniqKey="Reisdorph N" first="Nichole" last="Reisdorph">Nichole Reisdorph</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address: Nichole.Reisdorph@UCDenver.edu.</nlm:affiliation></affiliation></author><author><name sortKey="Armstrong, Michael" sort="Armstrong, Michael" uniqKey="Armstrong M" first="Michael" last="Armstrong">Michael Armstrong</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Powell, Roger" sort="Powell, Roger" uniqKey="Powell R" first="Roger" last="Powell">Roger Powell</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Quinn, Kevin" sort="Quinn, Kevin" uniqKey="Quinn K" first="Kevin" last="Quinn">Kevin Quinn</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Legg, Kevin" sort="Legg, Kevin" uniqKey="Legg K" first="Kevin" last="Legg">Kevin Legg</name><affiliation><nlm:affiliation>The Center for Forensic Science Research and Education, 2300 Stratford Avenue, Willow Grove, PA 19090, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Leung, Donald" sort="Leung, Donald" uniqKey="Leung D" first="Donald" last="Leung">Donald Leung</name><affiliation><nlm:affiliation>Department of Pediatrics, National Jewish Health, Denver, CO 80206, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Reisdorph, Rick" sort="Reisdorph, Rick" uniqKey="Reisdorph R" first="Rick" last="Reisdorph">Rick Reisdorph</name><affiliation><nlm:affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="eISSN">1873-376X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Case-Control Studies (MeSH)</term><term>Dermatitis, Atopic (metabolism)</term><term>Humans (MeSH)</term><term>Isotope Labeling (methods)</term><term>Limit of Detection (MeSH)</term><term>Linear Models (MeSH)</term><term>Peptides (analysis)</term><term>Proteomics (methods)</term><term>Reproducibility of Results (MeSH)</term><term>Skin (chemistry)</term><term>Tandem Mass Spectrometry (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Skin</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Dermatitis, Atopic</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Isotope Labeling</term><term>Proteomics</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Case-Control Studies</term><term>Humans</term><term>Limit of Detection</term><term>Linear Models</term><term>Reproducibility of Results</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previous work from our laboratories utilized a novel skin taping method and mass spectrometry-based proteomics to discover clinical biomarkers of skin conditions; these included atopic dermatitis, Staphylococcus aureus colonization, and eczema herpeticum. While suitable for discovery purposes, semi-quantitative proteomics is generally time-consuming and expensive. Furthermore, depending on the method used, discovery-based proteomics can result in high variation and inadequate sensitivity to detect low abundant peptides. Therefore, we strove to develop a rapid, sensitive, and reproducible method to quantitate disease-related proteins from skin tapings. We utilized isotopically-labeled peptides and tandem mass spectrometry to obtain absolute quantitation values on 14 peptides from 7 proteins; these proteins had shown previous importance in skin disease. The method demonstrated good reproducibility, dynamic range, and linearity (R<sup>2</sup> > 0.993) when n = 3 standards were analyzed across 0.05-2.5 pmol. The method was used to determine if differences exist between skin proteins in a small group of atopic versus non-atopic individuals (n = 12). While only minimal differences were found, peptides were detected in all samples and exhibited good correlation between peptides for 5 of the 7 proteins (R<sup>2</sup> = 0.71-0.98). This method can be applied to larger cohorts to further establish the relationships of these proteins to skin disease.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29601982</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-376X</ISSN><JournalIssue CitedMedium="Internet"><Volume>1084</Volume><PubDate><Year>2018</Year><Month>May</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</Title><ISOAbbreviation>J Chromatogr B Analyt Technol Biomed Life Sci</ISOAbbreviation></Journal><ArticleTitle>Quantitation of peptides from non-invasive skin tapings using isotope dilution and tandem mass spectrometry.</ArticleTitle><Pagination><MedlinePgn>132-140</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1570-0232(17)32215-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jchromb.2018.03.031</ELocationID><Abstract><AbstractText>Previous work from our laboratories utilized a novel skin taping method and mass spectrometry-based proteomics to discover clinical biomarkers of skin conditions; these included atopic dermatitis, Staphylococcus aureus colonization, and eczema herpeticum. While suitable for discovery purposes, semi-quantitative proteomics is generally time-consuming and expensive. Furthermore, depending on the method used, discovery-based proteomics can result in high variation and inadequate sensitivity to detect low abundant peptides. Therefore, we strove to develop a rapid, sensitive, and reproducible method to quantitate disease-related proteins from skin tapings. We utilized isotopically-labeled peptides and tandem mass spectrometry to obtain absolute quantitation values on 14 peptides from 7 proteins; these proteins had shown previous importance in skin disease. The method demonstrated good reproducibility, dynamic range, and linearity (R<sup>2</sup> > 0.993) when n = 3 standards were analyzed across 0.05-2.5 pmol. The method was used to determine if differences exist between skin proteins in a small group of atopic versus non-atopic individuals (n = 12). While only minimal differences were found, peptides were detected in all samples and exhibited good correlation between peptides for 5 of the 7 proteins (R<sup>2</sup> = 0.71-0.98). This method can be applied to larger cohorts to further establish the relationships of these proteins to skin disease.</AbstractText><CopyrightInformation>Copyright © 2017. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Reisdorph</LastName><ForeName>Nichole</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address: Nichole.Reisdorph@UCDenver.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Armstrong</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Powell</LastName><ForeName>Roger</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Quinn</LastName><ForeName>Kevin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Legg</LastName><ForeName>Kevin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>The Center for Forensic Science Research and Education, 2300 Stratford Avenue, Willow Grove, PA 19090, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leung</LastName><ForeName>Donald</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Pediatrics, National Jewish Health, Denver, CO 80206, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reisdorph</LastName><ForeName>Rick</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>KL2 TR001080</GrantID><Acronym>TR</Acronym><Agency>NCATS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>KL2 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UI="D010455">Peptides</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016022" MajorTopicYN="N">Case-Control Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003876" MajorTopicYN="N">Dermatitis, Atopic</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007553" MajorTopicYN="N">Isotope Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016014" MajorTopicYN="N">Linear Models</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012867" MajorTopicYN="N">Skin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Atopic dermatitis</Keyword><Keyword MajorTopicYN="N">Isotope dilution</Keyword><Keyword MajorTopicYN="N">Mass spectrometry</Keyword><Keyword MajorTopicYN="N">Proteomics</Keyword><Keyword MajorTopicYN="N">Quantitation</Keyword><Keyword MajorTopicYN="N">Skin tapings</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>12</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>03</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>3</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29601982</ArticleId><ArticleId IdType="pii">S1570-0232(17)32215-8</ArticleId><ArticleId 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