Amending an As/Pb contaminated soil with biochar, compost and iron grit: effect on Salix viminalis growth, root proteome profiles and metal(loid) accumulation indexes.
Identifieur interne : 000321 ( Main/Corpus ); précédent : 000320; suivant : 000322Amending an As/Pb contaminated soil with biochar, compost and iron grit: effect on Salix viminalis growth, root proteome profiles and metal(loid) accumulation indexes.
Auteurs : Manhattan Lebrun ; Elena De Zio ; Florie Miard ; Gabriella S. Scippa ; Giovanni Renzone ; Andrea Scaloni ; Sylvain Bourgerie ; Domenico Morabito ; Dalila TrupianoSource :
- Chemosphere [ 1879-1298 ] ; 2020.
English descriptors
- KwdEn :
- Arsenic (analysis), Arsenic (chemistry), Biodegradation, Environmental (MeSH), Charcoal (MeSH), Composting (MeSH), Environmental Restoration and Remediation (methods), Iron (MeSH), Lead (analysis), Lead (chemistry), Metals, Heavy (analysis), Proteome (MeSH), Proteomics (MeSH), Salix (physiology), Soil (MeSH), Soil Pollutants (analysis), Soil Pollutants (chemistry).
- MESH :
- chemical , analysis : Arsenic, Lead, Metals, Heavy, Soil Pollutants.
- chemical , chemistry : Arsenic, Lead, Soil Pollutants.
- methods : Environmental Restoration and Remediation.
- physiology : Salix.
- Biodegradation, Environmental, Charcoal, Composting, Iron, Proteome, Proteomics, Soil.
Abstract
There is currently a large amount of research being done into the phytoremediation of polluted soils. Plant installation in contaminated soils may require the application of soil amendments, such as biochar, compost and/or iron grit, which can improve the soil conditions and reduce the metal (loid) phytoavailability and mobility. The beneficial effects of these amendments on soil properties, plant growth and metal (loid) accumulation ability have already been described, although their effect on the plants response machinery has been poorly studied. This study aimed to assess the effect of these amendments on Salix viminalis growth and metal (loid) accumulation, as well as elucidating associated molecular mechanisms. The results showed that the amendment applications improved plant growth by three fold, except for the biochar plus iron combination. It also revealed that metal (loid)s were not effectively translocated from the roots to the shoots (translocation factors <1), their bioaccumulation peaked in the roots, and increased in the presence of iron-based amendments. Corresponding proteomic profiles revealed 34 protein spots differentially represented and suggested that plants counteracted metal (loid)-induced oxidative stress after the addition of biochar and/or compost by eliciting proper defense and signaling pathways, and by redirecting the metabolic fluxes towards primary and secondary metabolism. However, they did highlight the occurrence of oxidative stress markers when the biochar plus iron amendment was applied, which could be both the cause and result of protein degradation impairment.
DOI: 10.1016/j.chemosphere.2019.125397
PubMed: 31812046
Links to Exploration step
pubmed:31812046Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Amending an As/Pb contaminated soil with biochar, compost and iron grit: effect on Salix viminalis growth, root proteome profiles and metal(loid) accumulation indexes.</title><author><name sortKey="Lebrun, Manhattan" sort="Lebrun, Manhattan" uniqKey="Lebrun M" first="Manhattan" last="Lebrun">Manhattan Lebrun</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy; LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="De Zio, Elena" sort="De Zio, Elena" uniqKey="De Zio E" first="Elena" last="De Zio">Elena De Zio</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Miard, Florie" sort="Miard, Florie" uniqKey="Miard F" first="Florie" last="Miard">Florie Miard</name><affiliation><nlm:affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Scippa, Gabriella S" sort="Scippa, Gabriella S" uniqKey="Scippa G" first="Gabriella S" last="Scippa">Gabriella S. Scippa</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Renzone, Giovanni" sort="Renzone, Giovanni" uniqKey="Renzone G" first="Giovanni" last="Renzone">Giovanni Renzone</name><affiliation><nlm:affiliation>Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147, Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Scaloni, Andrea" sort="Scaloni, Andrea" uniqKey="Scaloni A" first="Andrea" last="Scaloni">Andrea Scaloni</name><affiliation><nlm:affiliation>Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147, Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Bourgerie, Sylvain" sort="Bourgerie, Sylvain" uniqKey="Bourgerie S" first="Sylvain" last="Bourgerie">Sylvain Bourgerie</name><affiliation><nlm:affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name><affiliation><nlm:affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Trupiano, Dalila" sort="Trupiano, Dalila" uniqKey="Trupiano D" first="Dalila" last="Trupiano">Dalila Trupiano</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy. Electronic address: dalila.trupiano@unimol.it.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31812046</idno><idno type="pmid">31812046</idno><idno type="doi">10.1016/j.chemosphere.2019.125397</idno><idno type="wicri:Area/Main/Corpus">000321</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000321</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Amending an As/Pb contaminated soil with biochar, compost and iron grit: effect on Salix viminalis growth, root proteome profiles and metal(loid) accumulation indexes.</title><author><name sortKey="Lebrun, Manhattan" sort="Lebrun, Manhattan" uniqKey="Lebrun M" first="Manhattan" last="Lebrun">Manhattan Lebrun</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy; LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="De Zio, Elena" sort="De Zio, Elena" uniqKey="De Zio E" first="Elena" last="De Zio">Elena De Zio</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Miard, Florie" sort="Miard, Florie" uniqKey="Miard F" first="Florie" last="Miard">Florie Miard</name><affiliation><nlm:affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Scippa, Gabriella S" sort="Scippa, Gabriella S" uniqKey="Scippa G" first="Gabriella S" last="Scippa">Gabriella S. Scippa</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Renzone, Giovanni" sort="Renzone, Giovanni" uniqKey="Renzone G" first="Giovanni" last="Renzone">Giovanni Renzone</name><affiliation><nlm:affiliation>Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147, Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Scaloni, Andrea" sort="Scaloni, Andrea" uniqKey="Scaloni A" first="Andrea" last="Scaloni">Andrea Scaloni</name><affiliation><nlm:affiliation>Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147, Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Bourgerie, Sylvain" sort="Bourgerie, Sylvain" uniqKey="Bourgerie S" first="Sylvain" last="Bourgerie">Sylvain Bourgerie</name><affiliation><nlm:affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name><affiliation><nlm:affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Trupiano, Dalila" sort="Trupiano, Dalila" uniqKey="Trupiano D" first="Dalila" last="Trupiano">Dalila Trupiano</name><affiliation><nlm:affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy. Electronic address: dalila.trupiano@unimol.it.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Chemosphere</title><idno type="eISSN">1879-1298</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arsenic (analysis)</term><term>Arsenic (chemistry)</term><term>Biodegradation, Environmental (MeSH)</term><term>Charcoal (MeSH)</term><term>Composting (MeSH)</term><term>Environmental Restoration and Remediation (methods)</term><term>Iron (MeSH)</term><term>Lead (analysis)</term><term>Lead (chemistry)</term><term>Metals, Heavy (analysis)</term><term>Proteome (MeSH)</term><term>Proteomics (MeSH)</term><term>Salix (physiology)</term><term>Soil (MeSH)</term><term>Soil Pollutants (analysis)</term><term>Soil Pollutants (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Arsenic</term><term>Lead</term><term>Metals, Heavy</term><term>Soil Pollutants</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arsenic</term><term>Lead</term><term>Soil Pollutants</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Environmental Restoration and Remediation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Salix</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Charcoal</term><term>Composting</term><term>Iron</term><term>Proteome</term><term>Proteomics</term><term>Soil</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is currently a large amount of research being done into the phytoremediation of polluted soils. Plant installation in contaminated soils may require the application of soil amendments, such as biochar, compost and/or iron grit, which can improve the soil conditions and reduce the metal (loid) phytoavailability and mobility. The beneficial effects of these amendments on soil properties, plant growth and metal (loid) accumulation ability have already been described, although their effect on the plants response machinery has been poorly studied. This study aimed to assess the effect of these amendments on Salix viminalis growth and metal (loid) accumulation, as well as elucidating associated molecular mechanisms. The results showed that the amendment applications improved plant growth by three fold, except for the biochar plus iron combination. It also revealed that metal (loid)s were not effectively translocated from the roots to the shoots (translocation factors <1), their bioaccumulation peaked in the roots, and increased in the presence of iron-based amendments. Corresponding proteomic profiles revealed 34 protein spots differentially represented and suggested that plants counteracted metal (loid)-induced oxidative stress after the addition of biochar and/or compost by eliciting proper defense and signaling pathways, and by redirecting the metabolic fluxes towards primary and secondary metabolism. However, they did highlight the occurrence of oxidative stress markers when the biochar plus iron amendment was applied, which could be both the cause and result of protein degradation impairment.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31812046</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1298</ISSN><JournalIssue CitedMedium="Internet"><Volume>244</Volume><PubDate><Year>2020</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>Amending an As/Pb contaminated soil with biochar, compost and iron grit: effect on Salix viminalis growth, root proteome profiles and metal(loid) accumulation indexes.</ArticleTitle><Pagination><MedlinePgn>125397</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0045-6535(19)32637-2</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chemosphere.2019.125397</ELocationID><Abstract><AbstractText>There is currently a large amount of research being done into the phytoremediation of polluted soils. Plant installation in contaminated soils may require the application of soil amendments, such as biochar, compost and/or iron grit, which can improve the soil conditions and reduce the metal (loid) phytoavailability and mobility. The beneficial effects of these amendments on soil properties, plant growth and metal (loid) accumulation ability have already been described, although their effect on the plants response machinery has been poorly studied. This study aimed to assess the effect of these amendments on Salix viminalis growth and metal (loid) accumulation, as well as elucidating associated molecular mechanisms. The results showed that the amendment applications improved plant growth by three fold, except for the biochar plus iron combination. It also revealed that metal (loid)s were not effectively translocated from the roots to the shoots (translocation factors <1), their bioaccumulation peaked in the roots, and increased in the presence of iron-based amendments. Corresponding proteomic profiles revealed 34 protein spots differentially represented and suggested that plants counteracted metal (loid)-induced oxidative stress after the addition of biochar and/or compost by eliciting proper defense and signaling pathways, and by redirecting the metabolic fluxes towards primary and secondary metabolism. However, they did highlight the occurrence of oxidative stress markers when the biochar plus iron amendment was applied, which could be both the cause and result of protein degradation impairment.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lebrun</LastName><ForeName>Manhattan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy; LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>De Zio</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miard</LastName><ForeName>Florie</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scippa</LastName><ForeName>Gabriella S</ForeName><Initials>GS</Initials><AffiliationInfo><Affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Renzone</LastName><ForeName>Giovanni</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147, Napoli, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scaloni</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147, Napoli, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bourgerie</LastName><ForeName>Sylvain</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morabito</LastName><ForeName>Domenico</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>LBLGC-EA 1207, INRA USC1328, Orléans University, Rue de Chartres, BP 6759, 45067, Orléans Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trupiano</LastName><ForeName>Dalila</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Biosciences and Territory, University of Molise, 86090, Pesche, IS, Italy. Electronic address: dalila.trupiano@unimol.it.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Chemosphere</MedlineTA><NlmUniqueID>0320657</NlmUniqueID><ISSNLinking>0045-6535</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C540010">biochar</NameOfSubstance></Chemical><Chemical><RegistryNumber>16291-96-6</RegistryNumber><NameOfSubstance UI="D002606">Charcoal</NameOfSubstance></Chemical><Chemical><RegistryNumber>2P299V784P</RegistryNumber><NameOfSubstance UI="D007854">Lead</NameOfSubstance></Chemical><Chemical><RegistryNumber>E1UOL152H7</RegistryNumber><NameOfSubstance UI="D007501">Iron</NameOfSubstance></Chemical><Chemical><RegistryNumber>N712M78A8G</RegistryNumber><NameOfSubstance UI="D001151">Arsenic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001151" MajorTopicYN="N">Arsenic</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002606" MajorTopicYN="N">Charcoal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000076282" MajorTopicYN="N">Composting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052918" MajorTopicYN="N">Environmental Restoration and Remediation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007501" MajorTopicYN="N">Iron</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007854" MajorTopicYN="N">Lead</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Amendment</Keyword><Keyword MajorTopicYN="N">Arsenic</Keyword><Keyword MajorTopicYN="N">Heavy metals</Keyword><Keyword MajorTopicYN="N">Lead</Keyword><Keyword MajorTopicYN="N">Phytoremediation</Keyword><Keyword MajorTopicYN="N">Phytostabilization</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31812046</ArticleId><ArticleId IdType="pii">S0045-6535(19)32637-2</ArticleId><ArticleId IdType="doi">10.1016/j.chemosphere.2019.125397</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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