Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress.
Identifieur interne : 000000 ( Main/Corpus ); suivant : 000001Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress.
Auteurs : Songlin Zhang ; Hongchun Chen ; Danni He ; Xinrui He ; Ya Yan ; Kejun Wu ; Hong WeiSource :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
Chelate induction of organic acids has been recognized to enhance metal uptake and translocation in plants, but the underlying mechanism remains unclear. In this study, seedlings of Salix variegata were hydroponically exposed to the combinations of Cd (0 and 50 μM) and three exogenous organic acids (100 μM of citric, tartaric, or malic acid). Plant biomass, antioxidant enzymes, non-protein thiol compounds (NPT) content, and the expression of candidate genes associated with Cd accumulation and tolerance were determined. Results showed that Cd significantly inhibited plant biomass but stimulated the activity of antioxidant enzymes in the roots and leaves, while the lipid peroxidation increased as well. Respective addition of three organic acids greatly enhanced plant resistance to oxidative stress and reduced the lipid peroxidation induced by Cd, with the effect of malic acid showing greatest. The addition of organic acids also significantly increased the content of glutathione in the root, further improving the antioxidant capacity and potential of phytochelatin biosynthesis. Moreover, Cd induced the expression level of candidate genes in roots of S. variegata. The addition of three organic acids not only promoted the expression of candidate genes but also drastically increased Cd accumulation in S. variegata. In summary, application of citric, tartaric, or malic acid alleviated Cd-imposed toxicity through the boost of enzymatic and non-enzymatic antioxidants and candidate gene expression, while their effects on Cd tolerance and accumulation of S. variegata differed.
DOI: 10.3389/fpls.2020.594352
PubMed: 33193554
PubMed Central: PMC7644951
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Under Cd Stress.</title><author><name sortKey="Zhang, Songlin" sort="Zhang, Songlin" uniqKey="Zhang S" first="Songlin" last="Zhang">Songlin Zhang</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Hongchun" sort="Chen, Hongchun" uniqKey="Chen H" first="Hongchun" last="Chen">Hongchun Chen</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Guizhou Provincial Water Conservancy Research Institute, Guiyang, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Danni" sort="He, Danni" uniqKey="He D" first="Danni" last="He">Danni He</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Xinrui" sort="He, Xinrui" uniqKey="He X" first="Xinrui" last="He">Xinrui He</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yan, Ya" sort="Yan, Ya" uniqKey="Yan Y" first="Ya" last="Yan">Ya Yan</name><affiliation><nlm:affiliation>Guizhou Provincial Water Conservancy Research Institute, Guiyang, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Kejun" sort="Wu, Kejun" uniqKey="Wu K" first="Kejun" last="Wu">Kejun Wu</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wei, Hong" sort="Wei, Hong" uniqKey="Wei H" first="Hong" last="Wei">Hong Wei</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33193554</idno><idno type="pmid">33193554</idno><idno type="doi">10.3389/fpls.2020.594352</idno><idno type="pmc">PMC7644951</idno><idno type="wicri:Area/Main/Corpus">000000</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000000</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of <i>Salix variegata</i> Franch. Under Cd Stress.</title><author><name sortKey="Zhang, Songlin" sort="Zhang, Songlin" uniqKey="Zhang S" first="Songlin" last="Zhang">Songlin Zhang</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Hongchun" sort="Chen, Hongchun" uniqKey="Chen H" first="Hongchun" last="Chen">Hongchun Chen</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Guizhou Provincial Water Conservancy Research Institute, Guiyang, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Danni" sort="He, Danni" uniqKey="He D" first="Danni" last="He">Danni He</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="He, Xinrui" sort="He, Xinrui" uniqKey="He X" first="Xinrui" last="He">Xinrui He</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yan, Ya" sort="Yan, Ya" uniqKey="Yan Y" first="Ya" last="Yan">Ya Yan</name><affiliation><nlm:affiliation>Guizhou Provincial Water Conservancy Research Institute, Guiyang, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Kejun" sort="Wu, Kejun" uniqKey="Wu K" first="Kejun" last="Wu">Kejun Wu</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wei, Hong" sort="Wei, Hong" uniqKey="Wei H" first="Hong" last="Wei">Hong Wei</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chelate induction of organic acids has been recognized to enhance metal uptake and translocation in plants, but the underlying mechanism remains unclear. In this study, seedlings of <i>Salix variegata</i> were hydroponically exposed to the combinations of Cd (0 and 50 μM) and three exogenous organic acids (100 μM of citric, tartaric, or malic acid). Plant biomass, antioxidant enzymes, non-protein thiol compounds (NPT) content, and the expression of candidate genes associated with Cd accumulation and tolerance were determined. Results showed that Cd significantly inhibited plant biomass but stimulated the activity of antioxidant enzymes in the roots and leaves, while the lipid peroxidation increased as well. Respective addition of three organic acids greatly enhanced plant resistance to oxidative stress and reduced the lipid peroxidation induced by Cd, with the effect of malic acid showing greatest. The addition of organic acids also significantly increased the content of glutathione in the root, further improving the antioxidant capacity and potential of phytochelatin biosynthesis. Moreover, Cd induced the expression level of candidate genes in roots of <i>S. variegata</i>. The addition of three organic acids not only promoted the expression of candidate genes but also drastically increased Cd accumulation in <i>S. variegata</i>. In summary, application of citric, tartaric, or malic acid alleviated Cd-imposed toxicity through the boost of enzymatic and non-enzymatic antioxidants and candidate gene expression, while their effects on Cd tolerance and accumulation of <i>S. variegata</i> differed.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33193554</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of <i>Salix variegata</i> Franch. Under Cd Stress.</ArticleTitle><Pagination><MedlinePgn>594352</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.594352</ELocationID><Abstract><AbstractText>Chelate induction of organic acids has been recognized to enhance metal uptake and translocation in plants, but the underlying mechanism remains unclear. In this study, seedlings of <i>Salix variegata</i> were hydroponically exposed to the combinations of Cd (0 and 50 μM) and three exogenous organic acids (100 μM of citric, tartaric, or malic acid). Plant biomass, antioxidant enzymes, non-protein thiol compounds (NPT) content, and the expression of candidate genes associated with Cd accumulation and tolerance were determined. Results showed that Cd significantly inhibited plant biomass but stimulated the activity of antioxidant enzymes in the roots and leaves, while the lipid peroxidation increased as well. Respective addition of three organic acids greatly enhanced plant resistance to oxidative stress and reduced the lipid peroxidation induced by Cd, with the effect of malic acid showing greatest. The addition of organic acids also significantly increased the content of glutathione in the root, further improving the antioxidant capacity and potential of phytochelatin biosynthesis. Moreover, Cd induced the expression level of candidate genes in roots of <i>S. variegata</i>. The addition of three organic acids not only promoted the expression of candidate genes but also drastically increased Cd accumulation in <i>S. variegata</i>. In summary, application of citric, tartaric, or malic acid alleviated Cd-imposed toxicity through the boost of enzymatic and non-enzymatic antioxidants and candidate gene expression, while their effects on Cd tolerance and accumulation of <i>S. variegata</i> differed.</AbstractText><CopyrightInformation>Copyright © 2020 Zhang, Chen, He, He, Yan, Wu and Wei.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Songlin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Hongchun</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guizhou Provincial Water Conservancy Research Institute, Guiyang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Danni</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Xinrui</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Ya</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Guizhou Provincial Water Conservancy Research Institute, Guiyang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Kejun</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Hong</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Salix variegata</Keyword><Keyword MajorTopicYN="N">antioxidant enzymatic system</Keyword><Keyword MajorTopicYN="N">cadmium</Keyword><Keyword MajorTopicYN="N">gene expression</Keyword><Keyword MajorTopicYN="N">non-protein sulfhydryl compounds</Keyword><Keyword MajorTopicYN="N">organic acid</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>08</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>16</Day><Hour>8</Hour><Minute>48</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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