Degradation of Aflatoxin B1 by a Sustainable Enzymatic Extract from Spent Mushroom Substrate of Pleurotus eryngii.
Identifieur interne : 000159 ( Main/Exploration ); précédent : 000158; suivant : 000160Degradation of Aflatoxin B1 by a Sustainable Enzymatic Extract from Spent Mushroom Substrate of Pleurotus eryngii.
Auteurs : Maria Teresa Branà [Italie] ; Lucrezia Sergio [Italie] ; Miriam Haidukowski [Italie] ; Antonio F. Logrieco [Italie] ; Claudio Altomare [Italie]Source :
- Toxins [ 2072-6651 ] ; 2020.
Abstract
Ligninolytic enzymes from white-rot fungi, such as laccase (Lac) and Mn-peroxidase (MnP), are able to degrade aflatoxin B1 (AFB1), the most harmful among the known mycotoxins. The high cost of purification of these enzymes has limited their implementation into practical technologies. Every year, tons of spent mushroom substrate (SMS) are produced as a by-product of edible mushroom cultivation, such as Pleurotus spp., and disposed at a cost for farmers. SMS may still bea source of ligninolytic enzymes useful for AFB1 degradation. The in vitro AFB1-degradative activity of an SMS crude extract (SMSE) was investigated. Results show that: (1) in SMSE, high Lac activity (4 U g-1dry matter) and low MnP activity (0.4 U g-1dry matter) were present; (2) after 1 d of incubation at 25 °C, the SMSE was able to degrade more than 50% of AFB1, whereas after 3 and 7 d of incubation, the percentage of degradation reached the values of 75% and 90%, respectively; (3) with increasing pH values, the degradation percentage increased, reaching 90% after 3 d at pH 8. Based on these results, SMS proved to be a suitable source of AFB1 degrading enzymes and the use of SMSE to detoxify AFB1 contaminated commodities appears conceivable.
DOI: 10.3390/toxins12010049
PubMed: 31947703
PubMed Central: PMC7020430
Affiliations:
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Amendola 122/O, 70126 Bari, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari</wicri:regionArea><wicri:noRegion>70126 Bari</wicri:noRegion></affiliation></author><author><name sortKey="Sergio, Lucrezia" sort="Sergio, Lucrezia" uniqKey="Sergio L" first="Lucrezia" last="Sergio">Lucrezia Sergio</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari</wicri:regionArea><wicri:noRegion>70126 Bari</wicri:noRegion></affiliation></author><author><name sortKey="Haidukowski, Miriam" sort="Haidukowski, Miriam" uniqKey="Haidukowski M" first="Miriam" last="Haidukowski">Miriam Haidukowski</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari</wicri:regionArea><wicri:noRegion>70126 Bari</wicri:noRegion></affiliation></author><author><name sortKey="Logrieco, Antonio F" sort="Logrieco, Antonio F" uniqKey="Logrieco A" first="Antonio F" last="Logrieco">Antonio F. Logrieco</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari</wicri:regionArea><wicri:noRegion>70126 Bari</wicri:noRegion></affiliation></author><author><name sortKey="Altomare, Claudio" sort="Altomare, Claudio" uniqKey="Altomare C" first="Claudio" last="Altomare">Claudio Altomare</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari</wicri:regionArea><wicri:noRegion>70126 Bari</wicri:noRegion></affiliation></author></analytic><series><title level="j">Toxins</title><idno type="eISSN">2072-6651</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ligninolytic enzymes from white-rot fungi, such as laccase (Lac) and Mn-peroxidase (MnP), are able to degrade aflatoxin B<sub>1</sub> (AFB1), the most harmful among the known mycotoxins. The high cost of purification of these enzymes has limited their implementation into practical technologies. Every year, tons of spent mushroom substrate (SMS) are produced as a by-product of edible mushroom cultivation, such as Pleurotus spp., and disposed at a cost for farmers. SMS may still bea source of ligninolytic enzymes useful for AFB1 degradation. The in vitro AFB1-degradative activity of an SMS crude extract (SMSE) was investigated. Results show that: (1) in SMSE, high Lac activity (4 U g<sup>-1</sup>dry matter) and low MnP activity (0.4 U g<sup>-1</sup>dry matter) were present; (2) after 1 d of incubation at 25 °C, the SMSE was able to degrade more than 50% of AFB1<sub>,</sub> whereas after 3 and 7 d of incubation, the percentage of degradation reached the values of 75% and 90%, respectively; (3) with increasing pH values, the degradation percentage increased, reaching 90% after 3 d at pH 8. Based on these results, SMS proved to be a suitable source of AFB1 degrading enzymes and the use of SMSE to detoxify AFB1 contaminated commodities appears conceivable.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31947703</PMID><DateRevised><Year>2020</Year><Month>05</Month><Day>08</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2072-6651</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>14</Day></PubDate></JournalIssue><Title>Toxins</Title><ISOAbbreviation>Toxins (Basel)</ISOAbbreviation></Journal><ArticleTitle>Degradation of Aflatoxin B<sub>1</sub> by a Sustainable Enzymatic Extract from Spent Mushroom Substrate of <i>Pleurotus eryngii</i>.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E49</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/toxins12010049</ELocationID><Abstract><AbstractText>Ligninolytic enzymes from white-rot fungi, such as laccase (Lac) and Mn-peroxidase (MnP), are able to degrade aflatoxin B<sub>1</sub> (AFB1), the most harmful among the known mycotoxins. The high cost of purification of these enzymes has limited their implementation into practical technologies. Every year, tons of spent mushroom substrate (SMS) are produced as a by-product of edible mushroom cultivation, such as Pleurotus spp., and disposed at a cost for farmers. SMS may still bea source of ligninolytic enzymes useful for AFB1 degradation. The in vitro AFB1-degradative activity of an SMS crude extract (SMSE) was investigated. Results show that: (1) in SMSE, high Lac activity (4 U g<sup>-1</sup>dry matter) and low MnP activity (0.4 U g<sup>-1</sup>dry matter) were present; (2) after 1 d of incubation at 25 °C, the SMSE was able to degrade more than 50% of AFB1<sub>,</sub> whereas after 3 and 7 d of incubation, the percentage of degradation reached the values of 75% and 90%, respectively; (3) with increasing pH values, the degradation percentage increased, reaching 90% after 3 d at pH 8. Based on these results, SMS proved to be a suitable source of AFB1 degrading enzymes and the use of SMSE to detoxify AFB1 contaminated commodities appears conceivable.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Branà</LastName><ForeName>Maria Teresa</ForeName><Initials>MT</Initials><AffiliationInfo><Affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sergio</LastName><ForeName>Lucrezia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haidukowski</LastName><ForeName>Miriam</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Logrieco</LastName><ForeName>Antonio F</ForeName><Initials>AF</Initials><AffiliationInfo><Affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Altomare</LastName><ForeName>Claudio</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Sciences of Food Production, National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Toxins (Basel)</MedlineTA><NlmUniqueID>101530765</NlmUniqueID><ISSNLinking>2072-6651</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Pleurotuseryngi</Keyword><Keyword MajorTopicYN="Y">aflatoxins</Keyword><Keyword MajorTopicYN="Y">crudeextract</Keyword><Keyword MajorTopicYN="Y">feed detoxification</Keyword><Keyword MajorTopicYN="Y">king oyster mushroom</Keyword><Keyword MajorTopicYN="Y">laccase</Keyword><Keyword MajorTopicYN="Y">spent mushroom substrate</Keyword></KeywordList><CoiStatement>The authors declare no conflicts of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31947703</ArticleId><ArticleId 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Logrieco</name><name sortKey="Sergio, Lucrezia" sort="Sergio, Lucrezia" uniqKey="Sergio L" first="Lucrezia" last="Sergio">Lucrezia Sergio</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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