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A full saturated linkage map of Picea abies including AFLP, SSR, ESTP, 5S rDNA and morphological markers.

Identifieur interne : 000282 ( Main/Exploration ); précédent : 000281; suivant : 000283

A full saturated linkage map of Picea abies including AFLP, SSR, ESTP, 5S rDNA and morphological markers.

Auteurs : V. Acheré [France] ; P. Faivre-Rampant ; S. Jeandroz ; G. Besnard ; T. Markussen ; A. Aragones ; M. Fladung ; E. Ritter ; J-M Favre

Source :

RBID : pubmed:14991106

Descripteurs français

English descriptors

Abstract

Based on an F(1) progeny of 73 individuals, two parental maps were constructed according to the double pseudo-test cross strategy. The paternal map contained 16 linkage groups for a total genetic length of 1,792 cM. The maternal map covered 1,920 cM, and consisted of 12 linkage groups. These parental maps were then integrated using 66 intercross markers. The resulting consensus map covered 2,035 cM and included 755 markers (661 AFLPs, 74 SSRs, 18 ESTPs, the 5S rDNA and the early cone formation trait) on 12 linkage groups, reflecting the haploid number of chromosomes of Picea abies. The average spacing between two adjacent markers was 2.6 cM. The presence of 39 of the SSR and/or ESTP markers from this consensus map on other published maps of different Picea and Pinus species allowed us to establish partial linkage group homologies across three P. abies maps (up to five common markers per linkage group). This first saturated linkage map of P. abies could be therefore used as a support for developing comparative genome mapping in conifers.

DOI: 10.1007/s00122-004-1587-y
PubMed: 14991106


Affiliations:


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Le document en format XML

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<term>DNA, Ribosomal (genetics)</term>
<term>Expressed Sequence Tags (MeSH)</term>
<term>Genetic Markers (genetics)</term>
<term>Genome, Plant (MeSH)</term>
<term>Genomics (MeSH)</term>
<term>Karyotyping (MeSH)</term>
<term>Microsatellite Repeats (genetics)</term>
<term>Phenotype (MeSH)</term>
<term>Picea (genetics)</term>
<term>Picea (physiology)</term>
<term>Polymorphism, Genetic (genetics)</term>
<term>RNA, Ribosomal, 5S (genetics)</term>
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<term>ADN ribosomique (génétique)</term>
<term>ARN ribosomique 5S (génétique)</term>
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<term>Caryotypage (MeSH)</term>
<term>Chromosomes de plante (génétique)</term>
<term>Génome végétal (MeSH)</term>
<term>Génomique (MeSH)</term>
<term>Marqueurs génétiques (génétique)</term>
<term>Phénotype (MeSH)</term>
<term>Picea (génétique)</term>
<term>Picea (physiologie)</term>
<term>Polymorphisme génétique (génétique)</term>
<term>Répétitions microsatellites (génétique)</term>
<term>Étiquettes de séquences exprimées (MeSH)</term>
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<term>Genetic Markers</term>
<term>RNA, Ribosomal, 5S</term>
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<term>ARN ribosomique 5S</term>
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<term>Chromosome Mapping</term>
<term>Expressed Sequence Tags</term>
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<term>Caryotypage</term>
<term>Génome végétal</term>
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<div type="abstract" xml:lang="en">Based on an F(1) progeny of 73 individuals, two parental maps were constructed according to the double pseudo-test cross strategy. The paternal map contained 16 linkage groups for a total genetic length of 1,792 cM. The maternal map covered 1,920 cM, and consisted of 12 linkage groups. These parental maps were then integrated using 66 intercross markers. The resulting consensus map covered 2,035 cM and included 755 markers (661 AFLPs, 74 SSRs, 18 ESTPs, the 5S rDNA and the early cone formation trait) on 12 linkage groups, reflecting the haploid number of chromosomes of Picea abies. The average spacing between two adjacent markers was 2.6 cM. The presence of 39 of the SSR and/or ESTP markers from this consensus map on other published maps of different Picea and Pinus species allowed us to establish partial linkage group homologies across three P. abies maps (up to five common markers per linkage group). This first saturated linkage map of P. abies could be therefore used as a support for developing comparative genome mapping in conifers.</div>
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<AbstractText>Based on an F(1) progeny of 73 individuals, two parental maps were constructed according to the double pseudo-test cross strategy. The paternal map contained 16 linkage groups for a total genetic length of 1,792 cM. The maternal map covered 1,920 cM, and consisted of 12 linkage groups. These parental maps were then integrated using 66 intercross markers. The resulting consensus map covered 2,035 cM and included 755 markers (661 AFLPs, 74 SSRs, 18 ESTPs, the 5S rDNA and the early cone formation trait) on 12 linkage groups, reflecting the haploid number of chromosomes of Picea abies. The average spacing between two adjacent markers was 2.6 cM. The presence of 39 of the SSR and/or ESTP markers from this consensus map on other published maps of different Picea and Pinus species allowed us to establish partial linkage group homologies across three P. abies maps (up to five common markers per linkage group). This first saturated linkage map of P. abies could be therefore used as a support for developing comparative genome mapping in conifers.</AbstractText>
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