The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to infection by Melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins.
Identifieur interne : 000263 ( Main/Corpus ); précédent : 000262; suivant : 000264The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to infection by Melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins.
Auteurs : Manoela Miranda ; Steven G. Ralph ; Robin Mellway ; Rick White ; Michele C. Heath ; Jörg Bohlmann ; C Peter ConstabelSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2007.
English descriptors
- KwdEn :
- Basidiomycota (growth & development), Flavonoids (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Hybridization, Genetic (MeSH), Molecular Structure (MeSH), Oligonucleotide Array Sequence Analysis (MeSH), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Leaves (microbiology), Populus (genetics), Populus (metabolism), Populus (microbiology), Proanthocyanidins (chemistry), Proanthocyanidins (metabolism), Signal Transduction (genetics), Signal Transduction (physiology), Time Factors (MeSH), Transcription, Genetic (MeSH).
- MESH :
- chemical , chemistry : Proanthocyanidins.
- chemical , metabolism : Flavonoids, Proanthocyanidins.
- genetics : Plant Leaves, Populus, Signal Transduction.
- growth & development : Basidiomycota.
- metabolism : Plant Leaves, Populus.
- microbiology : Plant Leaves, Populus.
- physiology : Signal Transduction.
- Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Hybridization, Genetic, Molecular Structure, Oligonucleotide Array Sequence Analysis, Time Factors, Transcription, Genetic.
Abstract
The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to poplar leaf rust (Melampsora medusae) infection was studied using the Populus 15.5K cDNA microarray. Pronounced changes in the transcriptome were observed, with approximately 20% of genes on the array showing either induction or repression of transcription within the 9-day infection timecourse. A small number of pathogen-defense genes encoding PR-1, chitinases, and other pathogenesis-related proteins were consistently upregulated throughout the experimental period, but most genes were affected only at individual timepoints. The largest number of changes in gene expression was observed late in the infection at 6 to 9 days postinoculation (dpi). At these timepoints, genes encoding enzymes required for proanthocyanidin (condensed tannin) synthesis were upregulated dramatically. Phytochemical analysis confirmed that, late in the infection, proanthocyanidin levels increased in infected leaves. Strongly M. medusae-repressed genes at 9 dpi included previously characterized wound- and herbivore-induced defense genes, which suggests antagonism between the tree responses to insect feeding and M. medusae infection. In this highly compatible plant-pathogen interaction, we postulate that the biotrophic pathogen evades detection and suppresses early host responses.
DOI: 10.1094/MPMI-20-7-0816
PubMed: 17601169
Links to Exploration step
pubmed:17601169Le document en format XML
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<author><name sortKey="Miranda, Manoela" sort="Miranda, Manoela" uniqKey="Miranda M" first="Manoela" last="Miranda">Manoela Miranda</name>
<affiliation><nlm:affiliation>Centre for Forest Biology and Department of Biology, University of Victoria, Victoria, BC, Canada.</nlm:affiliation>
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<author><name sortKey="Ralph, Steven G" sort="Ralph, Steven G" uniqKey="Ralph S" first="Steven G" last="Ralph">Steven G. Ralph</name>
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<author><name sortKey="Mellway, Robin" sort="Mellway, Robin" uniqKey="Mellway R" first="Robin" last="Mellway">Robin Mellway</name>
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<author><name sortKey="White, Rick" sort="White, Rick" uniqKey="White R" first="Rick" last="White">Rick White</name>
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<author><name sortKey="Heath, Michele C" sort="Heath, Michele C" uniqKey="Heath M" first="Michele C" last="Heath">Michele C. Heath</name>
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<author><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name>
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<author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to infection by Melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins.</title>
<author><name sortKey="Miranda, Manoela" sort="Miranda, Manoela" uniqKey="Miranda M" first="Manoela" last="Miranda">Manoela Miranda</name>
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<author><name sortKey="Mellway, Robin" sort="Mellway, Robin" uniqKey="Mellway R" first="Robin" last="Mellway">Robin Mellway</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (growth & development)</term>
<term>Flavonoids (metabolism)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Hybridization, Genetic (MeSH)</term>
<term>Molecular Structure (MeSH)</term>
<term>Oligonucleotide Array Sequence Analysis (MeSH)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Leaves (microbiology)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Populus (microbiology)</term>
<term>Proanthocyanidins (chemistry)</term>
<term>Proanthocyanidins (metabolism)</term>
<term>Signal Transduction (genetics)</term>
<term>Signal Transduction (physiology)</term>
<term>Time Factors (MeSH)</term>
<term>Transcription, Genetic (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Proanthocyanidins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Flavonoids</term>
<term>Proanthocyanidins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term>
<term>Populus</term>
<term>Signal Transduction</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term>
<term>Populus</term>
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<term>Populus</term>
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<term>Genes, Plant</term>
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<front><div type="abstract" xml:lang="en">The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to poplar leaf rust (Melampsora medusae) infection was studied using the Populus 15.5K cDNA microarray. Pronounced changes in the transcriptome were observed, with approximately 20% of genes on the array showing either induction or repression of transcription within the 9-day infection timecourse. A small number of pathogen-defense genes encoding PR-1, chitinases, and other pathogenesis-related proteins were consistently upregulated throughout the experimental period, but most genes were affected only at individual timepoints. The largest number of changes in gene expression was observed late in the infection at 6 to 9 days postinoculation (dpi). At these timepoints, genes encoding enzymes required for proanthocyanidin (condensed tannin) synthesis were upregulated dramatically. Phytochemical analysis confirmed that, late in the infection, proanthocyanidin levels increased in infected leaves. Strongly M. medusae-repressed genes at 9 dpi included previously characterized wound- and herbivore-induced defense genes, which suggests antagonism between the tree responses to insect feeding and M. medusae infection. In this highly compatible plant-pathogen interaction, we postulate that the biotrophic pathogen evades detection and suppresses early host responses.</div>
</front>
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<Abstract><AbstractText>The transcriptional response of hybrid poplar (Populus trichocarpa x P. deltoides) to poplar leaf rust (Melampsora medusae) infection was studied using the Populus 15.5K cDNA microarray. Pronounced changes in the transcriptome were observed, with approximately 20% of genes on the array showing either induction or repression of transcription within the 9-day infection timecourse. A small number of pathogen-defense genes encoding PR-1, chitinases, and other pathogenesis-related proteins were consistently upregulated throughout the experimental period, but most genes were affected only at individual timepoints. The largest number of changes in gene expression was observed late in the infection at 6 to 9 days postinoculation (dpi). At these timepoints, genes encoding enzymes required for proanthocyanidin (condensed tannin) synthesis were upregulated dramatically. Phytochemical analysis confirmed that, late in the infection, proanthocyanidin levels increased in infected leaves. Strongly M. medusae-repressed genes at 9 dpi included previously characterized wound- and herbivore-induced defense genes, which suggests antagonism between the tree responses to insect feeding and M. medusae infection. In this highly compatible plant-pathogen interaction, we postulate that the biotrophic pathogen evades detection and suppresses early host responses.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Miranda</LastName>
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