The glutathione peroxidase gene family of Lotus japonicus: characterization of genomic clones, expression analyses and immunolocalization in legumes.
Identifieur interne : 000253 ( Main/Corpus ); précédent : 000252; suivant : 000254The glutathione peroxidase gene family of Lotus japonicus: characterization of genomic clones, expression analyses and immunolocalization in legumes.
Auteurs : Javier Ramos ; Manuel A. Matamoros ; Loreto Naya ; Euan K. James ; Nicolas Rouhier ; Shusei Sato ; Satoshi Tabata ; Manuel BecanaSource :
- The New phytologist [ 1469-8137 ] ; 2009.
English descriptors
- KwdEn :
- Adaptation, Biological (genetics), Amino Acid Sequence (MeSH), Fabaceae (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Glutathione Peroxidase (genetics), Glutathione Peroxidase (metabolism), Immunoblotting (MeSH), Lotus (enzymology), Lotus (ultrastructure), Molecular Sequence Data (MeSH), Multigene Family (MeSH), RNA, Messenger (metabolism).
- MESH :
- chemical , genetics : Glutathione Peroxidase.
- enzymology : Lotus.
- genetics : Adaptation, Biological.
- chemical , metabolism : Glutathione Peroxidase, RNA, Messenger.
- ultrastructure : Lotus.
- Amino Acid Sequence, Fabaceae, Gene Expression, Gene Expression Regulation, Plant, Genes, Plant, Immunoblotting, Molecular Sequence Data, Multigene Family.
Abstract
Despite the multiple roles played by antioxidants in rhizobia-legume symbioses, little is known about glutathione peroxidases (GPXs) in legumes. Here the characterization of six GPX genes of Lotus japonicus is reported. Expression of GPX genes was analysed by quantitative reverse transcription-polymerase chain reaction in L. japonicus and Lotus corniculatus plants exposed to various treatments known to generate reactive oxygen and/or nitrogen species. LjGPX1 and LjGPX3 were the most abundantly expressed genes in leaves, roots and nodules. Compared with roots, LjGPX1 and LjGPX6 were highly expressed in leaves and LjGPX3 and LjGPX6 in nodules. In roots, salinity decreased GPX4 expression, aluminium decreased expression of the six genes, and cadmium caused up-regulation of GPX3, GPX4 and GPX5 after 1 h and down-regulation of GPX1, GPX2, GPX4 and GPX6 after 3-24 h. Exposure of roots to sodium nitroprusside (a nitric oxide donor) for 1 h increased the mRNA levels of GPX4 and GPX6 by 3.3- and 30-fold, respectively. Thereafter, the GPX6 mRNA level remained consistently higher than that of the control. Immunogold labelling revealed the presence of GPX proteins in root and nodule amyloplasts and in leaf chloroplasts of L. japonicus and other legumes. Labelling was associated with starch grains. These results underscore the differential regulation of GPX expression in response to cadmium, aluminium and nitric oxide, and strongly support a role for GPX6 and possibly other GPX genes in stress and/or metabolic signalling.
DOI: 10.1111/j.1469-8137.2008.02629.x
PubMed: 18826485
Links to Exploration step
pubmed:18826485Le document en format XML
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Matamoros</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Naya, Loreto" sort="Naya, Loreto" uniqKey="Naya L" first="Loreto" last="Naya">Loreto Naya</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="James, Euan K" sort="James, Euan K" uniqKey="James E" first="Euan K" last="James">Euan K. James</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Sato, Shusei" sort="Sato, Shusei" uniqKey="Sato S" first="Shusei" last="Sato">Shusei Sato</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Tabata, Satoshi" sort="Tabata, Satoshi" uniqKey="Tabata S" first="Satoshi" last="Tabata">Satoshi Tabata</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Becana, Manuel" sort="Becana, Manuel" uniqKey="Becana M" first="Manuel" last="Becana">Manuel Becana</name><affiliation><nlm:affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Biological (genetics)</term><term>Amino Acid Sequence (MeSH)</term><term>Fabaceae (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Glutathione Peroxidase (genetics)</term><term>Glutathione Peroxidase (metabolism)</term><term>Immunoblotting (MeSH)</term><term>Lotus (enzymology)</term><term>Lotus (ultrastructure)</term><term>Molecular Sequence Data (MeSH)</term><term>Multigene Family (MeSH)</term><term>RNA, Messenger (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutathione Peroxidase</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Lotus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adaptation, Biological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Peroxidase</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Lotus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Fabaceae</term><term>Gene Expression</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Immunoblotting</term><term>Molecular Sequence Data</term><term>Multigene Family</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Despite the multiple roles played by antioxidants in rhizobia-legume symbioses, little is known about glutathione peroxidases (GPXs) in legumes. Here the characterization of six GPX genes of Lotus japonicus is reported. Expression of GPX genes was analysed by quantitative reverse transcription-polymerase chain reaction in L. japonicus and Lotus corniculatus plants exposed to various treatments known to generate reactive oxygen and/or nitrogen species. LjGPX1 and LjGPX3 were the most abundantly expressed genes in leaves, roots and nodules. Compared with roots, LjGPX1 and LjGPX6 were highly expressed in leaves and LjGPX3 and LjGPX6 in nodules. In roots, salinity decreased GPX4 expression, aluminium decreased expression of the six genes, and cadmium caused up-regulation of GPX3, GPX4 and GPX5 after 1 h and down-regulation of GPX1, GPX2, GPX4 and GPX6 after 3-24 h. Exposure of roots to sodium nitroprusside (a nitric oxide donor) for 1 h increased the mRNA levels of GPX4 and GPX6 by 3.3- and 30-fold, respectively. Thereafter, the GPX6 mRNA level remained consistently higher than that of the control. Immunogold labelling revealed the presence of GPX proteins in root and nodule amyloplasts and in leaf chloroplasts of L. japonicus and other legumes. Labelling was associated with starch grains. These results underscore the differential regulation of GPX expression in response to cadmium, aluminium and nitric oxide, and strongly support a role for GPX6 and possibly other GPX genes in stress and/or metabolic signalling.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18826485</PMID><DateCompleted><Year>2009</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>181</Volume><Issue>1</Issue><PubDate><Year>2009</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The glutathione peroxidase gene family of Lotus japonicus: characterization of genomic clones, expression analyses and immunolocalization in legumes.</ArticleTitle><Pagination><MedlinePgn>103-114</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2008.02629.x</ELocationID><Abstract><AbstractText>Despite the multiple roles played by antioxidants in rhizobia-legume symbioses, little is known about glutathione peroxidases (GPXs) in legumes. Here the characterization of six GPX genes of Lotus japonicus is reported. Expression of GPX genes was analysed by quantitative reverse transcription-polymerase chain reaction in L. japonicus and Lotus corniculatus plants exposed to various treatments known to generate reactive oxygen and/or nitrogen species. LjGPX1 and LjGPX3 were the most abundantly expressed genes in leaves, roots and nodules. Compared with roots, LjGPX1 and LjGPX6 were highly expressed in leaves and LjGPX3 and LjGPX6 in nodules. In roots, salinity decreased GPX4 expression, aluminium decreased expression of the six genes, and cadmium caused up-regulation of GPX3, GPX4 and GPX5 after 1 h and down-regulation of GPX1, GPX2, GPX4 and GPX6 after 3-24 h. Exposure of roots to sodium nitroprusside (a nitric oxide donor) for 1 h increased the mRNA levels of GPX4 and GPX6 by 3.3- and 30-fold, respectively. Thereafter, the GPX6 mRNA level remained consistently higher than that of the control. Immunogold labelling revealed the presence of GPX proteins in root and nodule amyloplasts and in leaf chloroplasts of L. japonicus and other legumes. Labelling was associated with starch grains. These results underscore the differential regulation of GPX expression in response to cadmium, aluminium and nitric oxide, and strongly support a role for GPX6 and possibly other GPX genes in stress and/or metabolic signalling.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramos</LastName><ForeName>Javier</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Matamoros</LastName><ForeName>Manuel A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Naya</LastName><ForeName>Loreto</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>James</LastName><ForeName>Euan K</ForeName><Initials>EK</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rouhier</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sato</LastName><ForeName>Shusei</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tabata</LastName><ForeName>Satoshi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Becana</LastName><ForeName>Manuel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 13034, 50080 Zaragoza, Spain;College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK;UMR 1136 Tree-Microbes Interactions, IFR110, Nancy University, Vandoeuvre-les-Nancy, France;Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>09</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.9</RegistryNumber><NameOfSubstance UI="D005979">Glutathione Peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000220" MajorTopicYN="N">Adaptation, Biological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007887" MajorTopicYN="N">Fabaceae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005979" MajorTopicYN="N">Glutathione Peroxidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015151" MajorTopicYN="N">Immunoblotting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000070116" MajorTopicYN="N">Lotus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="N">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>2</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18826485</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2008.02629.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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