CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.
Identifieur interne : 000216 ( Main/Curation ); précédent : 000215; suivant : 000217CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.
Auteurs : Florent Cipriani [France] ; Martin Röwer ; Christophe Landret ; Ulrich Zander ; Franck Felisaz ; José Antonio MárquezSource :
- Acta crystallographica. Section D, Biological crystallography [ 1399-0047 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Cristallisation (instrumentation), Cristallisation (méthodes), Cristallographie aux rayons X (MeSH), Diffusion de rayonnements (MeSH), Femelle (MeSH), Lasers (MeSH), Lysozyme (composition chimique), Poulets (MeSH), Protéines végétales (composition chimique), Robotique (instrumentation), Robotique (méthodes).
- MESH :
- composition chimique : Lysozyme, Protéines végétales.
- méthodes : Cristallisation, Robotique.
- instrumentation : Animaux, Cristallisation, Cristallographie aux rayons X, Diffusion de rayonnements, Femelle, Lasers, Poulets, Robotique.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Muramidase, Plant Proteins.
- instrumentation : Crystallization, Robotics.
- methods : Crystallization, Robotics.
- Animals, Chickens, Crystallography, X-Ray, Female, Lasers, Scattering, Radiation.
Abstract
The use of automated systems for crystallization and X-ray data collection is now widespread. However, these two steps are separated by the need to transfer crystals from crystallization supports to X-ray data-collection supports, which is a difficult manual operation. Here, a new approach is proposed called CrystalDirect (CD) which enables full automation of the crystal-harvesting process. In this approach, crystals are grown on ultrathin films in a newly designed vapour-diffusion crystallization plate and are recovered by excision of the film through laser-induced photoablation. The film pieces containing crystals are then directly attached to a pin for X-ray data collection. This new method eliminates the delicate step of `crystal fishing', thereby enabling full automation of the crystal-mounting process. Additional advantages of this approach include the absence of mechanical stress and that it facilitates handling of microcrystals. The CD crystallization plates are also suitable for in situ crystal screening with minimal X-ray background. This method could enable the operational integration of highly automated crystallization and data-collection facilities, minimizing the delay between crystal identification and diffraction measurements. It can also contribute significantly to the advancement of challenging projects that require the systematic testing of large numbers of crystals.
DOI: 10.1107/S0907444912031459
PubMed: 22993093
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.</title><author><name sortKey="Cipriani, Florent" sort="Cipriani, Florent" uniqKey="Cipriani F" first="Florent" last="Cipriani">Florent Cipriani</name><affiliation wicri:level="1"><nlm:affiliation>Grenoble Outstation, European Molecular Biology Laboratory, 6 Rue Jules Horowitz, 38042 Grenoble, France. cipriani@embl.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Grenoble Outstation, European Molecular Biology Laboratory, 6 Rue Jules Horowitz, 38042 Grenoble</wicri:regionArea></affiliation></author><author><name sortKey="Rower, Martin" sort="Rower, Martin" uniqKey="Rower M" first="Martin" last="Röwer">Martin Röwer</name></author><author><name sortKey="Landret, Christophe" sort="Landret, Christophe" uniqKey="Landret C" first="Christophe" last="Landret">Christophe Landret</name></author><author><name sortKey="Zander, Ulrich" sort="Zander, Ulrich" uniqKey="Zander U" first="Ulrich" last="Zander">Ulrich Zander</name></author><author><name sortKey="Felisaz, Franck" sort="Felisaz, Franck" uniqKey="Felisaz F" first="Franck" last="Felisaz">Franck Felisaz</name></author><author><name sortKey="Marquez, Jose Antonio" sort="Marquez, Jose Antonio" uniqKey="Marquez J" first="José Antonio" last="Márquez">José Antonio Márquez</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22993093</idno><idno type="pmid">22993093</idno><idno type="doi">10.1107/S0907444912031459</idno><idno type="wicri:Area/Main/Corpus">000216</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000216</idno><idno type="wicri:Area/Main/Curation">000216</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000216</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.</title><author><name sortKey="Cipriani, Florent" sort="Cipriani, Florent" uniqKey="Cipriani F" first="Florent" last="Cipriani">Florent Cipriani</name><affiliation wicri:level="1"><nlm:affiliation>Grenoble Outstation, European Molecular Biology Laboratory, 6 Rue Jules Horowitz, 38042 Grenoble, France. cipriani@embl.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Grenoble Outstation, European Molecular Biology Laboratory, 6 Rue Jules Horowitz, 38042 Grenoble</wicri:regionArea></affiliation></author><author><name sortKey="Rower, Martin" sort="Rower, Martin" uniqKey="Rower M" first="Martin" last="Röwer">Martin Röwer</name></author><author><name sortKey="Landret, Christophe" sort="Landret, Christophe" uniqKey="Landret C" first="Christophe" last="Landret">Christophe Landret</name></author><author><name sortKey="Zander, Ulrich" sort="Zander, Ulrich" uniqKey="Zander U" first="Ulrich" last="Zander">Ulrich Zander</name></author><author><name sortKey="Felisaz, Franck" sort="Felisaz, Franck" uniqKey="Felisaz F" first="Franck" last="Felisaz">Franck Felisaz</name></author><author><name sortKey="Marquez, Jose Antonio" sort="Marquez, Jose Antonio" uniqKey="Marquez J" first="José Antonio" last="Márquez">José Antonio Márquez</name></author></analytic><series><title level="j">Acta crystallographica. Section D, Biological crystallography</title><idno type="eISSN">1399-0047</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Chickens (MeSH)</term><term>Crystallization (instrumentation)</term><term>Crystallization (methods)</term><term>Crystallography, X-Ray (MeSH)</term><term>Female (MeSH)</term><term>Lasers (MeSH)</term><term>Muramidase (chemistry)</term><term>Plant Proteins (chemistry)</term><term>Robotics (instrumentation)</term><term>Robotics (methods)</term><term>Scattering, Radiation (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Cristallisation (instrumentation)</term><term>Cristallisation (méthodes)</term><term>Cristallographie aux rayons X (MeSH)</term><term>Diffusion de rayonnements (MeSH)</term><term>Femelle (MeSH)</term><term>Lasers (MeSH)</term><term>Lysozyme (composition chimique)</term><term>Poulets (MeSH)</term><term>Protéines végétales (composition chimique)</term><term>Robotique (instrumentation)</term><term>Robotique (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Muramidase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lysozyme</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Crystallization</term><term>Robotics</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Crystallization</term><term>Robotics</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Cristallisation</term><term>Robotique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Crystallography, X-Ray</term><term>Female</term><term>Lasers</term><term>Scattering, Radiation</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Animaux</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Diffusion de rayonnements</term><term>Femelle</term><term>Lasers</term><term>Poulets</term><term>Robotique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The use of automated systems for crystallization and X-ray data collection is now widespread. However, these two steps are separated by the need to transfer crystals from crystallization supports to X-ray data-collection supports, which is a difficult manual operation. Here, a new approach is proposed called CrystalDirect (CD) which enables full automation of the crystal-harvesting process. In this approach, crystals are grown on ultrathin films in a newly designed vapour-diffusion crystallization plate and are recovered by excision of the film through laser-induced photoablation. The film pieces containing crystals are then directly attached to a pin for X-ray data collection. This new method eliminates the delicate step of `crystal fishing', thereby enabling full automation of the crystal-mounting process. Additional advantages of this approach include the absence of mechanical stress and that it facilitates handling of microcrystals. The CD crystallization plates are also suitable for in situ crystal screening with minimal X-ray background. This method could enable the operational integration of highly automated crystallization and data-collection facilities, minimizing the delay between crystal identification and diffraction measurements. It can also contribute significantly to the advancement of challenging projects that require the systematic testing of large numbers of crystals.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22993093</PMID><DateCompleted><Year>2013</Year><Month>01</Month><Day>21</Day></DateCompleted><DateRevised><Year>2012</Year><Month>09</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-0047</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>Pt 10</Issue><PubDate><Year>2012</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Acta crystallographica. Section D, Biological crystallography</Title><ISOAbbreviation>Acta Crystallogr D Biol Crystallogr</ISOAbbreviation></Journal><ArticleTitle>CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.</ArticleTitle><Pagination><MedlinePgn>1393-9</MedlinePgn></Pagination><Abstract><AbstractText>The use of automated systems for crystallization and X-ray data collection is now widespread. However, these two steps are separated by the need to transfer crystals from crystallization supports to X-ray data-collection supports, which is a difficult manual operation. Here, a new approach is proposed called CrystalDirect (CD) which enables full automation of the crystal-harvesting process. In this approach, crystals are grown on ultrathin films in a newly designed vapour-diffusion crystallization plate and are recovered by excision of the film through laser-induced photoablation. The film pieces containing crystals are then directly attached to a pin for X-ray data collection. This new method eliminates the delicate step of `crystal fishing', thereby enabling full automation of the crystal-mounting process. Additional advantages of this approach include the absence of mechanical stress and that it facilitates handling of microcrystals. The CD crystallization plates are also suitable for in situ crystal screening with minimal X-ray background. This method could enable the operational integration of highly automated crystallization and data-collection facilities, minimizing the delay between crystal identification and diffraction measurements. It can also contribute significantly to the advancement of challenging projects that require the systematic testing of large numbers of crystals.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cipriani</LastName><ForeName>Florent</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Grenoble Outstation, European Molecular Biology Laboratory, 6 Rue Jules Horowitz, 38042 Grenoble, France. cipriani@embl.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Röwer</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Landret</LastName><ForeName>Christophe</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Zander</LastName><ForeName>Ulrich</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Felisaz</LastName><ForeName>Franck</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Márquez</LastName><ForeName>José Antonio</ForeName><Initials>JA</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>PDB</DataBankName><AccessionNumberList><AccessionNumber>4AXR</AccessionNumber><AccessionNumber>4AXT</AccessionNumber><AccessionNumber>4AXU</AccessionNumber><AccessionNumber>4B0D</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Acta Crystallogr D Biol Crystallogr</MedlineTA><NlmUniqueID>9305878</NlmUniqueID><ISSNLinking>0907-4449</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>53850-34-3</RegistryNumber><NameOfSubstance UI="C003427">thaumatin protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="C421864">hen egg lysozyme</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.17</RegistryNumber><NameOfSubstance UI="D009113">Muramidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="N">Crystallization</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007834" MajorTopicYN="Y">Lasers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009113" MajorTopicYN="N">Muramidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012371" MajorTopicYN="N">Robotics</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012542" MajorTopicYN="N">Scattering, Radiation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>07</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22993093</ArticleId><ArticleId IdType="pii">S0907444912031459</ArticleId><ArticleId IdType="doi">10.1107/S0907444912031459</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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