Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.
Identifieur interne : 000079 ( Main/Curation ); précédent : 000078; suivant : 000080Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.
Auteurs : Jose M. Martin-Garcia [États-Unis] ; Chelsie E. Conrad [États-Unis] ; Garrett Nelson [États-Unis] ; Natasha Stander [États-Unis] ; Nadia A. Zatsepin [États-Unis] ; James Zook [États-Unis] ; Lan Zhu [États-Unis] ; James Geiger [États-Unis] ; Eugene Chun [États-Unis] ; David Kissick [États-Unis] ; Mark C. Hilgart [États-Unis] ; Craig Ogata [États-Unis] ; Andrii Ishchenko [États-Unis] ; Nirupa Nagaratnam [États-Unis] ; Shatabdi Roy-Chowdhury [États-Unis] ; Jesse Coe [États-Unis] ; Ganesh Subramanian [États-Unis] ; Alexander Schaffer [États-Unis] ; Daniel James [Suisse] ; Gihan Ketwala [États-Unis] ; Nagarajan Venugopalan [États-Unis] ; Shenglan Xu [États-Unis] ; Stephen Corcoran [États-Unis] ; Dale Ferguson [États-Unis] ; Uwe Weierstall [États-Unis] ; John C H. Spence [États-Unis] ; Vadim Cherezov [États-Unis] ; Petra Fromme [États-Unis] ; Robert F. Fischetti [États-Unis] ; Wei Liu [États-Unis]Source :
- IUCrJ [ 2052-2525 ] ; 2017.
Abstract
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX). As a result, the number of SMX experiments is growing rapidly, with a dozen experiments reported so far. Here, the first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported. Microcrystals (5-20 µm) of a wide variety of proteins, including lysozyme, thaumatin, phycocyanin, the human A2A adenosine receptor (A2AAR), the soluble fragment of the membrane lipoprotein Flpp3 and proteinase K, were screened. Crystals suspended in lipidic cubic phase (LCP) or a high-molecular-weight poly(ethylene oxide) (PEO; molecular weight 8 000 000) were delivered to the beam using a high-viscosity injector. In-house data-reduction (hit-finding) software developed at APS as well as the SFX data-reduction and analysis software suites Cheetah and CrystFEL enabled efficient on-site SMX data monitoring, reduction and processing. Complete data sets were collected for A2AAR, phycocyanin, Flpp3, proteinase K and lysozyme, and the structures of A2AAR, phycocyanin, proteinase K and lysozyme were determined at 3.2, 3.1, 2.65 and 2.05 Å resolution, respectively. The data demonstrate the feasibility of serial millisecond crystallography from 5-20 µm crystals using a high-viscosity injector at APS. The resolution of the crystal structures obtained in this study was dictated by the current flux density and crystal size, but upcoming developments in beamline optics and the planned APS-U upgrade will increase the intensity by two orders of magnitude. These developments will enable structure determination from smaller and/or weakly diffracting microcrystals.
DOI: 10.1107/S205225251700570X
PubMed: 28875031
PubMed Central: PMC5571807
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000079
Links to Exploration step
pubmed:28875031Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.</title>
<author><name sortKey="Martin Garcia, Jose M" sort="Martin Garcia, Jose M" uniqKey="Martin Garcia J" first="Jose M" last="Martin-Garcia">Jose M. Martin-Garcia</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Conrad, Chelsie E" sort="Conrad, Chelsie E" uniqKey="Conrad C" first="Chelsie E" last="Conrad">Chelsie E. Conrad</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD 21702</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nelson, Garrett" sort="Nelson, Garrett" uniqKey="Nelson G" first="Garrett" last="Nelson">Garrett Nelson</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Stander, Natasha" sort="Stander, Natasha" uniqKey="Stander N" first="Natasha" last="Stander">Natasha Stander</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zatsepin, Nadia A" sort="Zatsepin, Nadia A" uniqKey="Zatsepin N" first="Nadia A" last="Zatsepin">Nadia A. Zatsepin</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zook, James" sort="Zook, James" uniqKey="Zook J" first="James" last="Zook">James Zook</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhu, Lan" sort="Zhu, Lan" uniqKey="Zhu L" first="Lan" last="Zhu">Lan Zhu</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Geiger, James" sort="Geiger, James" uniqKey="Geiger J" first="James" last="Geiger">James Geiger</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chun, Eugene" sort="Chun, Eugene" uniqKey="Chun E" first="Eugene" last="Chun">Eugene Chun</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kissick, David" sort="Kissick, David" uniqKey="Kissick D" first="David" last="Kissick">David Kissick</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hilgart, Mark C" sort="Hilgart, Mark C" uniqKey="Hilgart M" first="Mark C" last="Hilgart">Mark C. Hilgart</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ogata, Craig" sort="Ogata, Craig" uniqKey="Ogata C" first="Craig" last="Ogata">Craig Ogata</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ishchenko, Andrii" sort="Ishchenko, Andrii" uniqKey="Ishchenko A" first="Andrii" last="Ishchenko">Andrii Ishchenko</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nagaratnam, Nirupa" sort="Nagaratnam, Nirupa" uniqKey="Nagaratnam N" first="Nirupa" last="Nagaratnam">Nirupa Nagaratnam</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Roy Chowdhury, Shatabdi" sort="Roy Chowdhury, Shatabdi" uniqKey="Roy Chowdhury S" first="Shatabdi" last="Roy-Chowdhury">Shatabdi Roy-Chowdhury</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Coe, Jesse" sort="Coe, Jesse" uniqKey="Coe J" first="Jesse" last="Coe">Jesse Coe</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Subramanian, Ganesh" sort="Subramanian, Ganesh" uniqKey="Subramanian G" first="Ganesh" last="Subramanian">Ganesh Subramanian</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schaffer, Alexander" sort="Schaffer, Alexander" uniqKey="Schaffer A" first="Alexander" last="Schaffer">Alexander Schaffer</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="James, Daniel" sort="James, Daniel" uniqKey="James D" first="Daniel" last="James">Daniel James</name>
<affiliation wicri:level="1"><nlm:affiliation>Paul Scherrer Institute, 5232 Villigen, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Paul Scherrer Institute, 5232 Villigen</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ketwala, Gihan" sort="Ketwala, Gihan" uniqKey="Ketwala G" first="Gihan" last="Ketwala">Gihan Ketwala</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Venugopalan, Nagarajan" sort="Venugopalan, Nagarajan" uniqKey="Venugopalan N" first="Nagarajan" last="Venugopalan">Nagarajan Venugopalan</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xu, Shenglan" sort="Xu, Shenglan" uniqKey="Xu S" first="Shenglan" last="Xu">Shenglan Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Corcoran, Stephen" sort="Corcoran, Stephen" uniqKey="Corcoran S" first="Stephen" last="Corcoran">Stephen Corcoran</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ferguson, Dale" sort="Ferguson, Dale" uniqKey="Ferguson D" first="Dale" last="Ferguson">Dale Ferguson</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Weierstall, Uwe" sort="Weierstall, Uwe" uniqKey="Weierstall U" first="Uwe" last="Weierstall">Uwe Weierstall</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Spence, John C H" sort="Spence, John C H" uniqKey="Spence J" first="John C H" last="Spence">John C H. Spence</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cherezov, Vadim" sort="Cherezov, Vadim" uniqKey="Cherezov V" first="Vadim" last="Cherezov">Vadim Cherezov</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fromme, Petra" sort="Fromme, Petra" uniqKey="Fromme P" first="Petra" last="Fromme">Petra Fromme</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fischetti, Robert F" sort="Fischetti, Robert F" uniqKey="Fischetti R" first="Robert F" last="Fischetti">Robert F. Fischetti</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:28875031</idno>
<idno type="pmid">28875031</idno>
<idno type="doi">10.1107/S205225251700570X</idno>
<idno type="pmc">PMC5571807</idno>
<idno type="wicri:Area/Main/Corpus">000079</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000079</idno>
<idno type="wicri:Area/Main/Curation">000079</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000079</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.</title>
<author><name sortKey="Martin Garcia, Jose M" sort="Martin Garcia, Jose M" uniqKey="Martin Garcia J" first="Jose M" last="Martin-Garcia">Jose M. Martin-Garcia</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Conrad, Chelsie E" sort="Conrad, Chelsie E" uniqKey="Conrad C" first="Chelsie E" last="Conrad">Chelsie E. Conrad</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD 21702</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nelson, Garrett" sort="Nelson, Garrett" uniqKey="Nelson G" first="Garrett" last="Nelson">Garrett Nelson</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Stander, Natasha" sort="Stander, Natasha" uniqKey="Stander N" first="Natasha" last="Stander">Natasha Stander</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zatsepin, Nadia A" sort="Zatsepin, Nadia A" uniqKey="Zatsepin N" first="Nadia A" last="Zatsepin">Nadia A. Zatsepin</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zook, James" sort="Zook, James" uniqKey="Zook J" first="James" last="Zook">James Zook</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhu, Lan" sort="Zhu, Lan" uniqKey="Zhu L" first="Lan" last="Zhu">Lan Zhu</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Geiger, James" sort="Geiger, James" uniqKey="Geiger J" first="James" last="Geiger">James Geiger</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chun, Eugene" sort="Chun, Eugene" uniqKey="Chun E" first="Eugene" last="Chun">Eugene Chun</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kissick, David" sort="Kissick, David" uniqKey="Kissick D" first="David" last="Kissick">David Kissick</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hilgart, Mark C" sort="Hilgart, Mark C" uniqKey="Hilgart M" first="Mark C" last="Hilgart">Mark C. Hilgart</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ogata, Craig" sort="Ogata, Craig" uniqKey="Ogata C" first="Craig" last="Ogata">Craig Ogata</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ishchenko, Andrii" sort="Ishchenko, Andrii" uniqKey="Ishchenko A" first="Andrii" last="Ishchenko">Andrii Ishchenko</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nagaratnam, Nirupa" sort="Nagaratnam, Nirupa" uniqKey="Nagaratnam N" first="Nirupa" last="Nagaratnam">Nirupa Nagaratnam</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Roy Chowdhury, Shatabdi" sort="Roy Chowdhury, Shatabdi" uniqKey="Roy Chowdhury S" first="Shatabdi" last="Roy-Chowdhury">Shatabdi Roy-Chowdhury</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Coe, Jesse" sort="Coe, Jesse" uniqKey="Coe J" first="Jesse" last="Coe">Jesse Coe</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Subramanian, Ganesh" sort="Subramanian, Ganesh" uniqKey="Subramanian G" first="Ganesh" last="Subramanian">Ganesh Subramanian</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schaffer, Alexander" sort="Schaffer, Alexander" uniqKey="Schaffer A" first="Alexander" last="Schaffer">Alexander Schaffer</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="James, Daniel" sort="James, Daniel" uniqKey="James D" first="Daniel" last="James">Daniel James</name>
<affiliation wicri:level="1"><nlm:affiliation>Paul Scherrer Institute, 5232 Villigen, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Paul Scherrer Institute, 5232 Villigen</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ketwala, Gihan" sort="Ketwala, Gihan" uniqKey="Ketwala G" first="Gihan" last="Ketwala">Gihan Ketwala</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Venugopalan, Nagarajan" sort="Venugopalan, Nagarajan" uniqKey="Venugopalan N" first="Nagarajan" last="Venugopalan">Nagarajan Venugopalan</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xu, Shenglan" sort="Xu, Shenglan" uniqKey="Xu S" first="Shenglan" last="Xu">Shenglan Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Corcoran, Stephen" sort="Corcoran, Stephen" uniqKey="Corcoran S" first="Stephen" last="Corcoran">Stephen Corcoran</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ferguson, Dale" sort="Ferguson, Dale" uniqKey="Ferguson D" first="Dale" last="Ferguson">Dale Ferguson</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Weierstall, Uwe" sort="Weierstall, Uwe" uniqKey="Weierstall U" first="Uwe" last="Weierstall">Uwe Weierstall</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Spence, John C H" sort="Spence, John C H" uniqKey="Spence J" first="John C H" last="Spence">John C H. Spence</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cherezov, Vadim" sort="Cherezov, Vadim" uniqKey="Cherezov V" first="Vadim" last="Cherezov">Vadim Cherezov</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fromme, Petra" sort="Fromme, Petra" uniqKey="Fromme P" first="Petra" last="Fromme">Petra Fromme</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fischetti, Robert F" sort="Fischetti, Robert F" uniqKey="Fischetti R" first="Robert F" last="Fischetti">Robert F. Fischetti</name>
<affiliation wicri:level="1"><nlm:affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">IUCrJ</title>
<idno type="ISSN">2052-2525</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX). As a result, the number of SMX experiments is growing rapidly, with a dozen experiments reported so far. Here, the first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported. Microcrystals (5-20 µm) of a wide variety of proteins, including lysozyme, thaumatin, phycocyanin, the human A<sub>2A</sub>
adenosine receptor (A<sub>2A</sub>
AR), the soluble fragment of the membrane lipoprotein Flpp3 and proteinase K, were screened. Crystals suspended in lipidic cubic phase (LCP) or a high-molecular-weight poly(ethylene oxide) (PEO; molecular weight 8 000 000) were delivered to the beam using a high-viscosity injector. In-house data-reduction (hit-finding) software developed at APS as well as the SFX data-reduction and analysis software suites <i>Cheetah</i>
and <i>CrystFEL</i>
enabled efficient on-site SMX data monitoring, reduction and processing. Complete data sets were collected for A<sub>2A</sub>
AR, phycocyanin, Flpp3, proteinase K and lysozyme, and the structures of A<sub>2A</sub>
AR, phycocyanin, proteinase K and lysozyme were determined at 3.2, 3.1, 2.65 and 2.05 Å resolution, respectively. The data demonstrate the feasibility of serial millisecond crystallography from 5-20 µm crystals using a high-viscosity injector at APS. The resolution of the crystal structures obtained in this study was dictated by the current flux density and crystal size, but upcoming developments in beamline optics and the planned APS-U upgrade will increase the intensity by two orders of magnitude. These developments will enable structure determination from smaller and/or weakly diffracting microcrystals.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28875031</PMID>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>29</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2052-2525</ISSN>
<JournalIssue CitedMedium="Print"><Volume>4</Volume>
<Issue>Pt 4</Issue>
<PubDate><Year>2017</Year>
<Month>Jul</Month>
<Day>01</Day>
</PubDate>
</JournalIssue>
<Title>IUCrJ</Title>
<ISOAbbreviation>IUCrJ</ISOAbbreviation>
</Journal>
<ArticleTitle>Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.</ArticleTitle>
<Pagination><MedlinePgn>439-454</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1107/S205225251700570X</ELocationID>
<Abstract><AbstractText>Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX). As a result, the number of SMX experiments is growing rapidly, with a dozen experiments reported so far. Here, the first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported. Microcrystals (5-20 µm) of a wide variety of proteins, including lysozyme, thaumatin, phycocyanin, the human A<sub>2A</sub>
adenosine receptor (A<sub>2A</sub>
AR), the soluble fragment of the membrane lipoprotein Flpp3 and proteinase K, were screened. Crystals suspended in lipidic cubic phase (LCP) or a high-molecular-weight poly(ethylene oxide) (PEO; molecular weight 8 000 000) were delivered to the beam using a high-viscosity injector. In-house data-reduction (hit-finding) software developed at APS as well as the SFX data-reduction and analysis software suites <i>Cheetah</i>
and <i>CrystFEL</i>
enabled efficient on-site SMX data monitoring, reduction and processing. Complete data sets were collected for A<sub>2A</sub>
AR, phycocyanin, Flpp3, proteinase K and lysozyme, and the structures of A<sub>2A</sub>
AR, phycocyanin, proteinase K and lysozyme were determined at 3.2, 3.1, 2.65 and 2.05 Å resolution, respectively. The data demonstrate the feasibility of serial millisecond crystallography from 5-20 µm crystals using a high-viscosity injector at APS. The resolution of the crystal structures obtained in this study was dictated by the current flux density and crystal size, but upcoming developments in beamline optics and the planned APS-U upgrade will increase the intensity by two orders of magnitude. These developments will enable structure determination from smaller and/or weakly diffracting microcrystals.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martin-Garcia</LastName>
<ForeName>Jose M</ForeName>
<Initials>JM</Initials>
<Identifier Source="ORCID">https://orcid.org/0000-0002-4558-3858</Identifier>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Conrad</LastName>
<ForeName>Chelsie E</ForeName>
<Initials>CE</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD 21702, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nelson</LastName>
<ForeName>Garrett</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Stander</LastName>
<ForeName>Natasha</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zatsepin</LastName>
<ForeName>Nadia A</ForeName>
<Initials>NA</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zook</LastName>
<ForeName>James</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Lan</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Geiger</LastName>
<ForeName>James</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chun</LastName>
<ForeName>Eugene</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kissick</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hilgart</LastName>
<ForeName>Mark C</ForeName>
<Initials>MC</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ogata</LastName>
<ForeName>Craig</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ishchenko</LastName>
<ForeName>Andrii</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nagaratnam</LastName>
<ForeName>Nirupa</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Roy-Chowdhury</LastName>
<ForeName>Shatabdi</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Coe</LastName>
<ForeName>Jesse</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Subramanian</LastName>
<ForeName>Ganesh</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schaffer</LastName>
<ForeName>Alexander</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>James</LastName>
<ForeName>Daniel</ForeName>
<Initials>D</Initials>
<Identifier Source="ORCID">https://orcid.org/0000-0002-8348-6661</Identifier>
<AffiliationInfo><Affiliation>Paul Scherrer Institute, 5232 Villigen, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ketwala</LastName>
<ForeName>Gihan</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Venugopalan</LastName>
<ForeName>Nagarajan</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Shenglan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Corcoran</LastName>
<ForeName>Stephen</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ferguson</LastName>
<ForeName>Dale</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Weierstall</LastName>
<ForeName>Uwe</ForeName>
<Initials>U</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Spence</LastName>
<ForeName>John C H</ForeName>
<Initials>JCH</Initials>
<AffiliationInfo><Affiliation>Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cherezov</LastName>
<ForeName>Vadim</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry, Bridge Institute, University of Southern California, 3430 South Vermont Avenue, MC 3303, Los Angeles, CA 90089, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fromme</LastName>
<ForeName>Petra</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fischetti</LastName>
<ForeName>Robert F</ForeName>
<Initials>RF</Initials>
<AffiliationInfo><Affiliation>Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>R01 GM108635</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R21 DA042298</GrantID>
<Acronym>DA</Acronym>
<Agency>NIDA NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>05</Month>
<Day>24</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>IUCrJ</MedlineTA>
<NlmUniqueID>101623101</NlmUniqueID>
<ISSNLinking>2052-2525</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Advanced Photon Source</Keyword>
<Keyword MajorTopicYN="N">high-viscosity injector</Keyword>
<Keyword MajorTopicYN="N">serial millisecond crystallography</Keyword>
<Keyword MajorTopicYN="N">synchrotron radiation</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>02</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>04</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>9</Month>
<Day>7</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>9</Month>
<Day>7</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>9</Month>
<Day>7</Day>
<Hour>6</Hour>
<Minute>1</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28875031</ArticleId>
<ArticleId IdType="doi">10.1107/S205225251700570X</ArticleId>
<ArticleId IdType="pii">it5011</ArticleId>
<ArticleId IdType="pmc">PMC5571807</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Structure. 2014 Jun 10;22(6):899-910</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24882744</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1985 Jul 20;184(2):257-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3928897</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2000 Aug 17;406(6797):752-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10963603</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Struct Biol. 2006 Oct;16(5):624-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16938450</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Struct Funct Genomics. 2012 Jun;13(2):81-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22418934</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUCrJ. 2014 Feb 10;1(Pt 2):87-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25075324</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Crystallogr. 2016 Mar 29;49(Pt 2):680-689</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27047311</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Opt Express. 2010 Mar 15;18(6):5713-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20389587</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2015 Oct;71(Pt 10):1987-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26457423</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phys Rev B Condens Matter Mater Phys. 2011 Dec 1;84(21):214111</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24089594</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1584-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23090408</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Struct Biol. 2016 Jan;72 (Pt 1):93-112</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26894538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19461840</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Adv. 2016 Sep 23;2(9):e1600292</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27679816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Sep;67(Pt 9):792-803</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21904032</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2015 Nov;71(Pt 11):2328-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26527148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2005 Mar 15;44(10):3955-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15751971</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):339-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20382986</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2450-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12604785</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Sep 27;108(39):16247-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21918110</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr A. 2011 Mar;67(Pt 2):131-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21325716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1231-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23793149</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Biochem Biophys. 2016 Jul 15;602:32-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27143509</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUCrJ. 2015 Jan 27;2(Pt 2):168-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25866654</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Crystallogr. 2014 Nov 18;47(Pt 6):1992-1999</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25484844</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUCrJ. 2014 May 30;1(Pt 4):204-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25075341</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1966 Dec;56(6):1746-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16591415</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2013 Jan;20(Pt 1):49-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23254655</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2007 Jan;14(Pt 1):1-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17211066</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2017 Jan 1;24(Pt 1):29-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28009544</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20551-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24297937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biochem Sci. 2014 May;39(5):233-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24767681</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUCrJ. 2015 Aug 04;2(Pt 5):545-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26306196</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2009 Mar;16(Pt 2):217-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19240333</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2005 May;12(Pt 3):257-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15840908</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Dec 3;462(7273):669-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19956261</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1195-203</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23793145</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Jan 11;339(6116):227-230</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23196907</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):176-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21358048</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2004 Dec 14;43(49):15595-603</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15581372</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2009;4(5):706-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19390528</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2015 Mar;22(2):239-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25723925</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1238-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26057665</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Jul 13;337(6091):232-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22798613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Phys Conf Ser. 2013;425:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25383086</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Phys Lipids. 1998 Sep;95(1):11-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9807807</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2014 Sep;9(9):2123-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25122522</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):387-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25664750</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods. 2004 Nov;34(3):415-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15325658</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2015 May;71(Pt 5):1184-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25945583</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21460454</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Rev Sci Instrum. 2012 Mar;83(3):035108</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22462961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUCrJ. 2015 Jun 30;2(Pt 4):421-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26177184</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2014;5:3309</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24525480</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUCrJ. 2014 Aug 25;1(Pt 5):349-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25295176</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Top Curr Chem. 2012;315:33-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21928014</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2012 Nov 16;287(47):39982-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23027878</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2011 Feb 3;470(7332):73-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21293373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1990 Feb 20;29(7):1669-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2184884</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12769-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25136092</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15572765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Pharmacol Sci. 2016 Dec;37(12 ):1055-1069</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27726881</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130316</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24914149</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Crystallogr. 2014 May 29;47(Pt 3):1118-1131</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24904246</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 2007 Jun;75(6):3089-101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17420240</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2008 Apr;64(Pt 4):425-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18391409</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Jul 20;337(6092):362-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22653729</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1997 Mar 7;275(5305):1471-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9045611</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19256-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23132943</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Anal Chem (Palo Alto Calif). 2011;4:419-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21469954</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phys Rev E Stat Nonlin Soft Matter Phys. 2015 Feb;91(2):022705</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25768529</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20057045</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2012 Jul;68(Pt 7):810-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22751666</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Synchrotron Radiat. 2011 Sep;18(Pt 5):717-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21862850</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/ThaumatinV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000079 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000079 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= ThaumatinV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:28875031 |texte= Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:28875031" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a ThaumatinV1
![]() | This area was generated with Dilib version V0.6.37. | ![]() |