Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.
Identifieur interne : 000443 ( Main/Corpus ); précédent : 000442; suivant : 000444Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.
Auteurs : Benjamin Petre ; Cécile Lorrain ; Diane G O. Saunders ; Joe Win ; Jan Sklenar ; Sébastien Duplessis ; Sophien KamounSource :
- Cellular microbiology [ 1462-5822 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Fungal Proteins, Virulence Factors.
- metabolism : Chloroplasts.
- microbiology : Plant Diseases, Populus.
- physiology : Basidiomycota.
- Molecular Mimicry, Protein Transport.
Abstract
Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified chloroplast-targeted protein 1 (CTP1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts and mitochondria. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins. Two members of that family, CTP2 and CTP3, also translocate in chloroplasts in an N-terminal signal-dependent manner. CTP1, CTP2 and CTP3 are cleaved when they accumulate in chloroplasts, while they remain intact when they do not translocate into chloroplasts. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells.
DOI: 10.1111/cmi.12530
PubMed: 26426202
Links to Exploration step
pubmed:26426202Le document en format XML
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<affiliation><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</nlm:affiliation>
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<author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name>
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<author><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name>
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<author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name>
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<series><title level="j">Cellular microbiology</title>
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<term>Chloroplasts (metabolism)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Molecular Mimicry (MeSH)</term>
<term>Plant Diseases (microbiology)</term>
<term>Populus (microbiology)</term>
<term>Protein Transport (MeSH)</term>
<term>Virulence Factors (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term>
<term>Virulence Factors</term>
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</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term>
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<front><div type="abstract" xml:lang="en">Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified chloroplast-targeted protein 1 (CTP1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts and mitochondria. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins. Two members of that family, CTP2 and CTP3, also translocate in chloroplasts in an N-terminal signal-dependent manner. CTP1, CTP2 and CTP3 are cleaved when they accumulate in chloroplasts, while they remain intact when they do not translocate into chloroplasts. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells. </div>
</front>
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<ArticleTitle>Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.</ArticleTitle>
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<Abstract><AbstractText>Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified chloroplast-targeted protein 1 (CTP1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts and mitochondria. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins. Two members of that family, CTP2 and CTP3, also translocate in chloroplasts in an N-terminal signal-dependent manner. CTP1, CTP2 and CTP3 are cleaved when they accumulate in chloroplasts, while they remain intact when they do not translocate into chloroplasts. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells. </AbstractText>
<CopyrightInformation>© 2015 John Wiley & Sons Ltd.</CopyrightInformation>
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