Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.
Identifieur interne : 000443 ( Main/Corpus ); précédent : 000442; suivant : 000444Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.
Auteurs : Benjamin Petre ; Cécile Lorrain ; Diane G O. Saunders ; Joe Win ; Jan Sklenar ; Sébastien Duplessis ; Sophien KamounSource :
- Cellular microbiology [ 1462-5822 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Fungal Proteins, Virulence Factors.
- metabolism : Chloroplasts.
- microbiology : Plant Diseases, Populus.
- physiology : Basidiomycota.
- Molecular Mimicry, Protein Transport.
Abstract
Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified chloroplast-targeted protein 1 (CTP1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts and mitochondria. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins. Two members of that family, CTP2 and CTP3, also translocate in chloroplasts in an N-terminal signal-dependent manner. CTP1, CTP2 and CTP3 are cleaved when they accumulate in chloroplasts, while they remain intact when they do not translocate into chloroplasts. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells.
DOI: 10.1111/cmi.12530
PubMed: 26426202
Links to Exploration step
pubmed:26426202Le document en format XML
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Saunders</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>The Genome Analysis Centre, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>The John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Sklenar, Jan" sort="Sklenar, Jan" uniqKey="Sklenar J" first="Jan" last="Sklenar">Jan Sklenar</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation><nlm:affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</nlm:affiliation></affiliation></author><author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26426202</idno><idno type="pmid">26426202</idno><idno type="doi">10.1111/cmi.12530</idno><idno type="wicri:Area/Main/Corpus">000443</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000443</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.</title><author><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</nlm:affiliation></affiliation></author><author><name sortKey="Saunders, Diane G O" sort="Saunders, Diane G O" uniqKey="Saunders D" first="Diane G O" last="Saunders">Diane G O. Saunders</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>The Genome Analysis Centre, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>The John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Sklenar, Jan" sort="Sklenar, Jan" uniqKey="Sklenar J" first="Jan" last="Sklenar">Jan Sklenar</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation><nlm:affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</nlm:affiliation></affiliation></author><author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name><affiliation><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Cellular microbiology</title><idno type="eISSN">1462-5822</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (physiology)</term><term>Chloroplasts (metabolism)</term><term>Fungal Proteins (metabolism)</term><term>Molecular Mimicry (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Populus (microbiology)</term><term>Protein Transport (MeSH)</term><term>Virulence Factors (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chloroplasts</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Mimicry</term><term>Protein Transport</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified chloroplast-targeted protein 1 (CTP1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts and mitochondria. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins. Two members of that family, CTP2 and CTP3, also translocate in chloroplasts in an N-terminal signal-dependent manner. CTP1, CTP2 and CTP3 are cleaved when they accumulate in chloroplasts, while they remain intact when they do not translocate into chloroplasts. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26426202</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-5822</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Cellular microbiology</Title><ISOAbbreviation>Cell Microbiol</ISOAbbreviation></Journal><ArticleTitle>Rust fungal effectors mimic host transit peptides to translocate into chloroplasts.</ArticleTitle><Pagination><MedlinePgn>453-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/cmi.12530</ELocationID><Abstract><AbstractText>Parasite effector proteins target various host cell compartments to alter host processes and promote infection. How effectors cross membrane-rich interfaces to reach these compartments is a major question in effector biology. Growing evidence suggests that effectors use molecular mimicry to subvert host cell machinery for protein sorting. We recently identified chloroplast-targeted protein 1 (CTP1), a candidate effector from the poplar leaf rust fungus Melampsora larici-populina that carries a predicted transit peptide and accumulates in chloroplasts and mitochondria. Here, we show that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts. CTP1 is part of a Melampsora-specific family of polymorphic secreted proteins. Two members of that family, CTP2 and CTP3, also translocate in chloroplasts in an N-terminal signal-dependent manner. CTP1, CTP2 and CTP3 are cleaved when they accumulate in chloroplasts, while they remain intact when they do not translocate into chloroplasts. Our findings reveal that fungi have evolved effector proteins that mimic plant-specific sorting signals to traffic within plant cells. </AbstractText><CopyrightInformation>© 2015 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petre</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lorrain</LastName><ForeName>Cécile</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saunders</LastName><ForeName>Diane G O</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Genome Analysis Centre, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Win</LastName><ForeName>Joe</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sklenar</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duplessis</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, 54280, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, 54506, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kamoun</LastName><ForeName>Sophien</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-0290-0315</Identifier><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cell Microbiol</MedlineTA><NlmUniqueID>100883691</NlmUniqueID><ISSNLinking>1462-5814</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018716" MajorTopicYN="N">Molecular Mimicry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>05</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>09</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>09</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26426202</ArticleId><ArticleId IdType="doi">10.1111/cmi.12530</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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