Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds.
Identifieur interne : 000027 ( Main/Exploration ); précédent : 000026; suivant : 000028Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds.
Auteurs : Karine De Guillen [France] ; Cécile Lorrain [France] ; Pascale Tsan [France] ; Philippe Barthe [France] ; Benjamin Petre [France] ; Natalya Saveleva [France] ; Nicolas Rouhier [France] ; Sébastien Duplessis [France] ; André Padilla [France] ; Arnaud Hecker [France]Source :
- Scientific reports [ 2045-2322 ] ; 2019.
Descripteurs français
- KwdFr :
- Basidiomycota (composition chimique), Basidiomycota (génétique), Conformation des protéines (MeSH), Cystine (composition chimique), Cystine (génétique), Génome fongique (MeSH), Maladies des plantes (microbiologie), Modèles moléculaires (MeSH), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Protéines recombinantes (composition chimique), Protéines recombinantes (génétique), Résonance magnétique nucléaire biomoléculaire (MeSH), Stabilité protéique (MeSH), Transporteurs nucléocytoplasmiques (composition chimique), Transporteurs nucléocytoplasmiques (génétique).
- MESH :
- composition chimique : Basidiomycota, Cystine, Protéines fongiques, Protéines recombinantes, Transporteurs nucléocytoplasmiques.
- génétique : Basidiomycota, Cystine, Protéines fongiques, Protéines recombinantes, Transporteurs nucléocytoplasmiques.
- microbiologie : Maladies des plantes.
- Conformation des protéines, Génome fongique, Modèles moléculaires, Résonance magnétique nucléaire biomoléculaire, Stabilité protéique.
English descriptors
- KwdEn :
- Basidiomycota (chemistry), Basidiomycota (genetics), Cystine (chemistry), Cystine (genetics), Fungal Proteins (chemistry), Fungal Proteins (genetics), Genome, Fungal (MeSH), Models, Molecular (MeSH), Nuclear Magnetic Resonance, Biomolecular (MeSH), Nucleocytoplasmic Transport Proteins (chemistry), Nucleocytoplasmic Transport Proteins (genetics), Plant Diseases (microbiology), Protein Conformation (MeSH), Protein Stability (MeSH), Recombinant Proteins (chemistry), Recombinant Proteins (genetics).
- MESH :
- chemical , chemistry : Cystine, Fungal Proteins, Nucleocytoplasmic Transport Proteins, Recombinant Proteins.
- chemistry : Basidiomycota.
- genetics : Basidiomycota, Cystine, Fungal Proteins, Nucleocytoplasmic Transport Proteins, Recombinant Proteins.
- microbiology : Plant Diseases.
- Genome, Fungal, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Protein Conformation, Protein Stability.
Abstract
Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus Melampsora larici-populina in Escherichia coli. We successfully purified and solved the three-dimensional structure of two proteins, MLP124266 and MLP124017, using NMR spectroscopy. Although both MLP124266 and MLP124017 show no sequence similarity with known proteins, they exhibit structural similarities to knottins, which are disulfide-rich small proteins characterized by intricate disulfide bridges, and to nuclear transport factor 2-like proteins, which are molecular containers involved in a wide range of functions, respectively. Interestingly, such structural folds have not been reported so far in pathogen effectors, indicating that MLP124266 and MLP124017 may bear novel functions related to pathogenicity. Our findings show that sequence-unrelated effectors can adopt folds similar to known proteins, and encourage the use of biochemical and structural approaches to functionally characterize effector candidates.
DOI: 10.1038/s41598-019-53816-9
PubMed: 31792250
PubMed Central: PMC6889267
Affiliations:
- France
- Grand Est, Languedoc-Roussillon, Lorraine (région), Occitanie (région administrative)
- Montpellier, Nancy
- Université de Lorraine
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Fungal Proteins (chemistry)</term>
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<term>Nucleocytoplasmic Transport Proteins (chemistry)</term>
<term>Nucleocytoplasmic Transport Proteins (genetics)</term>
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<term>Maladies des plantes (microbiologie)</term>
<term>Modèles moléculaires (MeSH)</term>
<term>Protéines fongiques (composition chimique)</term>
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<term>Protéines recombinantes (composition chimique)</term>
<term>Protéines recombinantes (génétique)</term>
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<term>Protéines recombinantes</term>
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<term>Protéines recombinantes</term>
<term>Transporteurs nucléocytoplasmiques</term>
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<term>Protein Stability</term>
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<front><div type="abstract" xml:lang="en">Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus Melampsora larici-populina in Escherichia coli. We successfully purified and solved the three-dimensional structure of two proteins, MLP124266 and MLP124017, using NMR spectroscopy. Although both MLP124266 and MLP124017 show no sequence similarity with known proteins, they exhibit structural similarities to knottins, which are disulfide-rich small proteins characterized by intricate disulfide bridges, and to nuclear transport factor 2-like proteins, which are molecular containers involved in a wide range of functions, respectively. Interestingly, such structural folds have not been reported so far in pathogen effectors, indicating that MLP124266 and MLP124017 may bear novel functions related to pathogenicity. Our findings show that sequence-unrelated effectors can adopt folds similar to known proteins, and encourage the use of biochemical and structural approaches to functionally characterize effector candidates.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31792250</PMID>
<DateCompleted><Year>2020</Year>
<Month>11</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>11</Month>
<Day>06</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>9</Volume>
<Issue>1</Issue>
<PubDate><Year>2019</Year>
<Month>12</Month>
<Day>02</Day>
</PubDate>
</JournalIssue>
<Title>Scientific reports</Title>
<ISOAbbreviation>Sci Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds.</ArticleTitle>
<Pagination><MedlinePgn>18084</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-53816-9</ELocationID>
<Abstract><AbstractText>Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus Melampsora larici-populina in Escherichia coli. We successfully purified and solved the three-dimensional structure of two proteins, MLP124266 and MLP124017, using NMR spectroscopy. Although both MLP124266 and MLP124017 show no sequence similarity with known proteins, they exhibit structural similarities to knottins, which are disulfide-rich small proteins characterized by intricate disulfide bridges, and to nuclear transport factor 2-like proteins, which are molecular containers involved in a wide range of functions, respectively. Interestingly, such structural folds have not been reported so far in pathogen effectors, indicating that MLP124266 and MLP124017 may bear novel functions related to pathogenicity. Our findings show that sequence-unrelated effectors can adopt folds similar to known proteins, and encourage the use of biochemical and structural approaches to functionally characterize effector candidates.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Guillen</LastName>
<ForeName>Karine</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Centre de Biochimie Structurale (CBS), INSERM U1054, CNRS UMR 5048, Univ Montpellier, F-34090, Montpellier, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lorrain</LastName>
<ForeName>Cécile</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tsan</LastName>
<ForeName>Pascale</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, CNRS, CRM2, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Barthe</LastName>
<ForeName>Philippe</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Centre de Biochimie Structurale (CBS), INSERM U1054, CNRS UMR 5048, Univ Montpellier, F-34090, Montpellier, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Petre</LastName>
<ForeName>Benjamin</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Saveleva</LastName>
<ForeName>Natalya</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rouhier</LastName>
<ForeName>Nicolas</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Duplessis</LastName>
<ForeName>Sébastien</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Padilla</LastName>
<ForeName>André</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Centre de Biochimie Structurale (CBS), INSERM U1054, CNRS UMR 5048, Univ Montpellier, F-34090, Montpellier, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hecker</LastName>
<ForeName>Arnaud</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine, INRA, IAM, F-54000, Nancy, France. arnaud.hecker@univ-lorraine.fr.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>12</Month>
<Day>02</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Sci Rep</MedlineTA>
<NlmUniqueID>101563288</NlmUniqueID>
<ISSNLinking>2045-2322</ISSNLinking>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029543">Nucleocytoplasmic Transport Proteins</NameOfSubstance>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003553" MajorTopicYN="N">Cystine</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016681" MajorTopicYN="N">Genome, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019906" MajorTopicYN="N">Nuclear Magnetic Resonance, Biomolecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029543" MajorTopicYN="N">Nucleocytoplasmic Transport Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055550" MajorTopicYN="N">Protein Stability</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
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<Month>09</Month>
<Day>04</Day>
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<Month>10</Month>
<Day>31</Day>
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<affiliations><list><country><li>France</li>
</country>
<region><li>Grand Est</li>
<li>Languedoc-Roussillon</li>
<li>Lorraine (région)</li>
<li>Occitanie (région administrative)</li>
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<tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="De Guillen, Karine" sort="De Guillen, Karine" uniqKey="De Guillen K" first="Karine" last="De Guillen">Karine De Guillen</name>
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<name sortKey="Barthe, Philippe" sort="Barthe, Philippe" uniqKey="Barthe P" first="Philippe" last="Barthe">Philippe Barthe</name>
<name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name>
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<name sortKey="Padilla, Andre" sort="Padilla, Andre" uniqKey="Padilla A" first="André" last="Padilla">André Padilla</name>
<name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name>
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