Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher.
Identifieur interne : 000006 ( Main/Exploration ); précédent : 000005; suivant : 000007Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher.
Auteurs : Jie Wei [République populaire de Chine] ; Liping Cui [République populaire de Chine] ; Na Zhang [République populaire de Chine] ; Dongdong Du [République populaire de Chine] ; Qingfang Meng [République populaire de Chine] ; Hongfei Yan [République populaire de Chine] ; Daqun Liu [République populaire de Chine] ; Wenxiang Yang [République populaire de Chine]Source :
- BMC genetics [ 1471-2156 ] ; 2020.
Abstract
BACKGROUND
Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks. (Pt) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates.
RESULTS
Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 h post inoculation (hpi). The mRNA was then sequenced, and a total of 2784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes.
CONCLUSION
The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.
DOI: 10.1186/s12863-020-00851-5
PubMed: 32345220
PubMed Central: PMC7189582
Affiliations:
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Na" sort="Zhang, Na" uniqKey="Zhang N" first="Na" last="Zhang">Na Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Du, Dongdong" sort="Du, Dongdong" uniqKey="Du D" first="Dongdong" last="Du">Dongdong Du</name><affiliation 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Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Daqun" sort="Liu, Daqun" uniqKey="Liu D" first="Daqun" last="Liu">Daqun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 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wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Cui, Liping" sort="Cui, Liping" uniqKey="Cui L" first="Liping" last="Cui">Liping Cui</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Na" sort="Zhang, Na" uniqKey="Zhang N" first="Na" last="Zhang">Na Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Du, Dongdong" sort="Du, Dongdong" uniqKey="Du D" first="Dongdong" last="Du">Dongdong Du</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Meng, Qingfang" sort="Meng, Qingfang" uniqKey="Meng Q" first="Qingfang" last="Meng">Qingfang Meng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Yan, Hongfei" sort="Yan, Hongfei" uniqKey="Yan H" first="Hongfei" last="Yan">Hongfei Yan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Daqun" sort="Liu, Daqun" uniqKey="Liu D" first="Daqun" last="Liu">Daqun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081</wicri:regionArea><wicri:noRegion>100081</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Wenxiang" sort="Yang, Wenxiang" uniqKey="Yang W" first="Wenxiang" last="Yang">Wenxiang Yang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China. wenxiangyang2003@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001</wicri:regionArea><wicri:noRegion>071001</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC genetics</title><idno type="eISSN">1471-2156</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks. (Pt) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 h post inoculation (hpi). The mRNA was then sequenced, and a total of 2784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32345220</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>06</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2156</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>04</Month><Day>28</Day></PubDate></JournalIssue><Title>BMC genetics</Title><ISOAbbreviation>BMC Genet</ISOAbbreviation></Journal><ArticleTitle>Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher.</ArticleTitle><Pagination><MedlinePgn>48</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12863-020-00851-5</ELocationID><Abstract><AbstractText Label="BACKGROUND">Wheat leaf rust is an important disease worldwide. Understanding the pathogenic molecular mechanism of Puccinia triticina Eriks. (Pt) and the inconstant toxic region is critical for managing the disease. The present study aimed to analyze the pathogenic divergence between Pt isolates.</AbstractText><AbstractText Label="RESULTS">Total RNA was extracted from the wheat cultivar Thatcher infected by two Pt isolates, Tc361_1 (THTT) and Tc284_2 (THTS), at 144 h post inoculation (hpi). The mRNA was then sequenced, and a total of 2784 differentially expressed genes (DEGs) were detected. Forty-five genes were specifically expressed in THTT; these genes included transcription initiation factors and genes with transmembrane transporter activity and other genes. Twenty-six genes were specifically expressed in THTS, including genes with GTPase activity, ABC transporters and other genes. Fifty-four differentially expressed candidate effectors were screened from the two isolates. Two candidate effectors were chosen and validated on tobacco, and the results showed that they could inhibit necrosis induced by BAX. qRT-PCR of 12 significant DEGs was carried out to validate that the results are similar to those of RNA-seq at 144 hpi, to show the expression levels of these DEGs in the early stage and to elucidate the differences in expression between the two Pt pathotypes.</AbstractText><AbstractText Label="CONCLUSION">The results obtained in this study showed that although the two pathotypes of THTT and THTS contribute similar virulence to wheat, there are a large number of genes participate in the interaction with the susceptible wheat cultivar Thatcher, and revealed the pathogenicity of rust is very complicated.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Jie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cui</LastName><ForeName>Liping</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Na</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Dongdong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Qingfang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Hongfei</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Daqun</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Wenxiang</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, 071001, China. wenxiangyang2003@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genet</MedlineTA><NlmUniqueID>100966978</NlmUniqueID><ISSNLinking>1471-2156</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Differential expression analysis</Keyword><Keyword MajorTopicYN="Y">Effector proteins</Keyword><Keyword MajorTopicYN="Y">RNA-seq analysis</Keyword><Keyword MajorTopicYN="Y">Wheat leaf rust</Keyword><Keyword MajorTopicYN="Y">qRT-PCR</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32345220</ArticleId><ArticleId IdType="doi">10.1186/s12863-020-00851-5</ArticleId><ArticleId IdType="pii">10.1186/s12863-020-00851-5</ArticleId><ArticleId 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Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wei, Jie" sort="Wei, Jie" uniqKey="Wei J" first="Jie" last="Wei">Jie Wei</name></noRegion><name sortKey="Cui, Liping" sort="Cui, Liping" uniqKey="Cui L" first="Liping" last="Cui">Liping Cui</name><name sortKey="Du, Dongdong" sort="Du, Dongdong" uniqKey="Du D" first="Dongdong" last="Du">Dongdong Du</name><name sortKey="Liu, Daqun" sort="Liu, Daqun" uniqKey="Liu D" first="Daqun" last="Liu">Daqun Liu</name><name sortKey="Meng, Qingfang" sort="Meng, Qingfang" uniqKey="Meng Q" first="Qingfang" last="Meng">Qingfang Meng</name><name sortKey="Yan, Hongfei" sort="Yan, Hongfei" uniqKey="Yan H" first="Hongfei" last="Yan">Hongfei Yan</name><name sortKey="Yang, Wenxiang" sort="Yang, Wenxiang" uniqKey="Yang W" first="Wenxiang" last="Yang">Wenxiang Yang</name><name sortKey="Zhang, Na" sort="Zhang, Na" uniqKey="Zhang N" first="Na" last="Zhang">Na 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