Stem rust spores elicit rapid RPG1 phosphorylation.
Identifieur interne : 000143 ( Main/Corpus ); précédent : 000142; suivant : 000144Stem rust spores elicit rapid RPG1 phosphorylation.
Auteurs : Jayaveeramuthu Nirmala ; Tom Drader ; Xianming Chen ; Brian Steffenson ; Andris KleinhofsSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Hordeum.
- metabolism : Hordeum, Plant Proteins.
- microbiology : Hordeum, Plant Diseases.
- physiology : Fungi, Gene Expression Regulation, Plant.
- Host-Pathogen Interactions, Phosphorylation.
Abstract
Stem rust threatens cereal production worldwide. Understanding the mechanism by which durable resistance genes, such as Rpg1, function is critical. We show that the RPG1 protein is phosphorylated within 5 min by exposure to spores from avirulent but not virulent races of stem rust. Transgenic mutants encoding an RPG1 protein with an in vitro inactive kinase domain fail to phosphorylate RPG1 in vivo and are susceptible to stem rust, demonstrating that phosphorylation is a prerequisite for disease resistance. Protein kinase inhibitors prevent RPG1 phosphorylation and result in susceptibility to stem rust, providing further evidence for the importance of phosphorylation in disease resistance. We conclude that phosphorylation of the RPG1 protein by the kinase activity of the pK2 domain induced by the interaction with an unknown pathogen spore product is required for resistance to the avirulent stem rust races. The pseudokinase pK1 domain is required for disease resistance but not phosphorylation. The very rapid phosphorylation of RPG1 suggests that an effector is already present in or on the stem rust urediniospores when they are placed on the leaf surface. However, spores must be alive, as determined by their ability to germinate, in order to elicit RPG1 phosphorylation.
DOI: 10.1094/MPMI-06-10-0136
PubMed: 20653415
Links to Exploration step
pubmed:20653415Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stem rust spores elicit rapid RPG1 phosphorylation.</title><author><name sortKey="Nirmala, Jayaveeramuthu" sort="Nirmala, Jayaveeramuthu" uniqKey="Nirmala J" first="Jayaveeramuthu" last="Nirmala">Jayaveeramuthu Nirmala</name><affiliation><nlm:affiliation>Department of Crop and Soil Sciences, Washington State University, Pullman 99164, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Drader, Tom" sort="Drader, Tom" uniqKey="Drader T" first="Tom" last="Drader">Tom Drader</name></author><author><name sortKey="Chen, Xianming" sort="Chen, Xianming" uniqKey="Chen X" first="Xianming" last="Chen">Xianming Chen</name></author><author><name sortKey="Steffenson, Brian" sort="Steffenson, Brian" uniqKey="Steffenson B" first="Brian" last="Steffenson">Brian Steffenson</name></author><author><name sortKey="Kleinhofs, Andris" sort="Kleinhofs, Andris" uniqKey="Kleinhofs A" first="Andris" last="Kleinhofs">Andris Kleinhofs</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20653415</idno><idno type="pmid">20653415</idno><idno type="doi">10.1094/MPMI-06-10-0136</idno><idno type="wicri:Area/Main/Corpus">000143</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000143</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Stem rust spores elicit rapid RPG1 phosphorylation.</title><author><name sortKey="Nirmala, Jayaveeramuthu" sort="Nirmala, Jayaveeramuthu" uniqKey="Nirmala J" first="Jayaveeramuthu" last="Nirmala">Jayaveeramuthu Nirmala</name><affiliation><nlm:affiliation>Department of Crop and Soil Sciences, Washington State University, Pullman 99164, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Drader, Tom" sort="Drader, Tom" uniqKey="Drader T" first="Tom" last="Drader">Tom Drader</name></author><author><name sortKey="Chen, Xianming" sort="Chen, Xianming" uniqKey="Chen X" first="Xianming" last="Chen">Xianming Chen</name></author><author><name sortKey="Steffenson, Brian" sort="Steffenson, Brian" uniqKey="Steffenson B" first="Brian" last="Steffenson">Brian Steffenson</name></author><author><name sortKey="Kleinhofs, Andris" sort="Kleinhofs, Andris" uniqKey="Kleinhofs A" first="Andris" last="Kleinhofs">Andris Kleinhofs</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungi (physiology)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Hordeum (genetics)</term><term>Hordeum (metabolism)</term><term>Hordeum (microbiology)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Phosphorylation (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hordeum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hordeum</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hordeum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fungi</term><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Host-Pathogen Interactions</term><term>Phosphorylation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Stem rust threatens cereal production worldwide. Understanding the mechanism by which durable resistance genes, such as Rpg1, function is critical. We show that the RPG1 protein is phosphorylated within 5 min by exposure to spores from avirulent but not virulent races of stem rust. Transgenic mutants encoding an RPG1 protein with an in vitro inactive kinase domain fail to phosphorylate RPG1 in vivo and are susceptible to stem rust, demonstrating that phosphorylation is a prerequisite for disease resistance. Protein kinase inhibitors prevent RPG1 phosphorylation and result in susceptibility to stem rust, providing further evidence for the importance of phosphorylation in disease resistance. We conclude that phosphorylation of the RPG1 protein by the kinase activity of the pK2 domain induced by the interaction with an unknown pathogen spore product is required for resistance to the avirulent stem rust races. The pseudokinase pK1 domain is required for disease resistance but not phosphorylation. The very rapid phosphorylation of RPG1 suggests that an effector is already present in or on the stem rust urediniospores when they are placed on the leaf surface. However, spores must be alive, as determined by their ability to germinate, in order to elicit RPG1 phosphorylation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20653415</PMID><DateCompleted><Year>2011</Year><Month>01</Month><Day>11</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>23</Volume><Issue>12</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Stem rust spores elicit rapid RPG1 phosphorylation.</ArticleTitle><Pagination><MedlinePgn>1635-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-06-10-0136</ELocationID><Abstract><AbstractText>Stem rust threatens cereal production worldwide. Understanding the mechanism by which durable resistance genes, such as Rpg1, function is critical. We show that the RPG1 protein is phosphorylated within 5 min by exposure to spores from avirulent but not virulent races of stem rust. Transgenic mutants encoding an RPG1 protein with an in vitro inactive kinase domain fail to phosphorylate RPG1 in vivo and are susceptible to stem rust, demonstrating that phosphorylation is a prerequisite for disease resistance. Protein kinase inhibitors prevent RPG1 phosphorylation and result in susceptibility to stem rust, providing further evidence for the importance of phosphorylation in disease resistance. We conclude that phosphorylation of the RPG1 protein by the kinase activity of the pK2 domain induced by the interaction with an unknown pathogen spore product is required for resistance to the avirulent stem rust races. The pseudokinase pK1 domain is required for disease resistance but not phosphorylation. The very rapid phosphorylation of RPG1 suggests that an effector is already present in or on the stem rust urediniospores when they are placed on the leaf surface. However, spores must be alive, as determined by their ability to germinate, in order to elicit RPG1 phosphorylation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nirmala</LastName><ForeName>Jayaveeramuthu</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Sciences, Washington State University, Pullman 99164, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Drader</LastName><ForeName>Tom</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xianming</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Steffenson</LastName><ForeName>Brian</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Kleinhofs</LastName><ForeName>Andris</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001467" MajorTopicYN="N">Hordeum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010766" MajorTopicYN="N">Phosphorylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>7</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>7</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>1</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20653415</ArticleId><ArticleId IdType="doi">10.1094/MPMI-06-10-0136</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RustEffectorV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000143 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000143 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RustEffectorV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:20653415
|texte= Stem rust spores elicit rapid RPG1 phosphorylation.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:20653415" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a RustEffectorV1
| This area was generated with Dilib version V0.6.37. |  |