Proteome analysis of wheat leaf rust fungus, Puccinia triticina, infection structures enriched for haustoria.
Identifieur interne : 000138 ( Main/Corpus ); précédent : 000137; suivant : 000139Proteome analysis of wheat leaf rust fungus, Puccinia triticina, infection structures enriched for haustoria.
Auteurs : Xiao Song ; Christof Rampitsch ; Bahram Soltani ; Wayne Mauthe ; Rob Linning ; Travis Banks ; Brent Mccallum ; Guus BakkerenSource :
- Proteomics [ 1615-9861 ] ; 2011.
English descriptors
- KwdEn :
- Basidiomycota (chemistry), Basidiomycota (genetics), Centrifugation, Density Gradient (MeSH), Cluster Analysis (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Expressed Sequence Tags (chemistry), Fungal Proteins (chemistry), Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Host-Parasite Interactions (MeSH), Mass Spectrometry (MeSH), Plant Diseases (microbiology), Plant Leaves (microbiology), Proteome (genetics), Proteome (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Analysis (MeSH), Sequence Homology (MeSH), Triticum (microbiology), Up-Regulation (MeSH).
- MESH :
- chemical , chemistry : Fungal Proteins.
- chemistry : Basidiomycota, Expressed Sequence Tags.
- genetics : Basidiomycota, Fungal Proteins, Proteome.
- chemical , metabolism : Proteome.
- microbiology : Plant Diseases, Plant Leaves, Triticum.
- Centrifugation, Density Gradient, Cluster Analysis, Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Fungal, Host-Parasite Interactions, Mass Spectrometry, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, Sequence Homology, Up-Regulation.
Abstract
Puccinia triticina (Pt) is a representative of several cereal-infecting rust fungal pathogens of major economic importance world wide. Upon entry through leaf stomata, these fungi establish intracellular haustoria, crucial feeding structures. We report the first proteome of infection structures from parasitized wheat leaves, enriched for haustoria through filtration and sucrose density centrifugation. 2-D PAGE MS/MS and gel-based LC-MS (GeLC-MS) were used to separate proteins. Generated spectra were compared with a partial proteome predicted from a preliminary Pt genome and generated ESTs, to a comprehensive genome-predicted protein complement from the related wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt) and to various plant resources. We identified over 260 fungal proteins, 16 of which matched peptides from Pgt. Based on bioinformatic analyses and/or the presence of a signal peptide, at least 50 proteins were predicted to be secreted. Among those, six have effector protein signatures, some are related and the respective genes of several seem to belong to clusters. Many ribosomal structural proteins, proteins involved in energy, general metabolism and transport were detected. Measuring gene expression over several life cycle stages of ten representative candidates using quantitative RT-PCR, all were shown to be strongly upregulated and four expressed solely upon infection.
DOI: 10.1002/pmic.201000014
PubMed: 21280219
Links to Exploration step
pubmed:21280219Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteome analysis of wheat leaf rust fungus, Puccinia triticina, infection structures enriched for haustoria.</title><author><name sortKey="Song, Xiao" sort="Song, Xiao" uniqKey="Song X" first="Xiao" last="Song">Xiao Song</name><affiliation><nlm:affiliation>Agriculture & Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Rampitsch, Christof" sort="Rampitsch, Christof" uniqKey="Rampitsch C" first="Christof" last="Rampitsch">Christof Rampitsch</name></author><author><name sortKey="Soltani, Bahram" sort="Soltani, Bahram" uniqKey="Soltani B" first="Bahram" last="Soltani">Bahram Soltani</name></author><author><name sortKey="Mauthe, Wayne" sort="Mauthe, Wayne" uniqKey="Mauthe W" first="Wayne" last="Mauthe">Wayne Mauthe</name></author><author><name sortKey="Linning, Rob" sort="Linning, Rob" uniqKey="Linning R" first="Rob" last="Linning">Rob Linning</name></author><author><name sortKey="Banks, Travis" sort="Banks, Travis" uniqKey="Banks T" first="Travis" last="Banks">Travis Banks</name></author><author><name sortKey="Mccallum, Brent" sort="Mccallum, Brent" uniqKey="Mccallum B" first="Brent" last="Mccallum">Brent Mccallum</name></author><author><name sortKey="Bakkeren, Guus" sort="Bakkeren, Guus" uniqKey="Bakkeren G" first="Guus" last="Bakkeren">Guus Bakkeren</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21280219</idno><idno type="pmid">21280219</idno><idno type="doi">10.1002/pmic.201000014</idno><idno type="wicri:Area/Main/Corpus">000138</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000138</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteome analysis of wheat leaf rust fungus, Puccinia triticina, infection structures enriched for haustoria.</title><author><name sortKey="Song, Xiao" sort="Song, Xiao" uniqKey="Song X" first="Xiao" last="Song">Xiao Song</name><affiliation><nlm:affiliation>Agriculture & Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Rampitsch, Christof" sort="Rampitsch, Christof" uniqKey="Rampitsch C" first="Christof" last="Rampitsch">Christof Rampitsch</name></author><author><name sortKey="Soltani, Bahram" sort="Soltani, Bahram" uniqKey="Soltani B" first="Bahram" last="Soltani">Bahram Soltani</name></author><author><name sortKey="Mauthe, Wayne" sort="Mauthe, Wayne" uniqKey="Mauthe W" first="Wayne" last="Mauthe">Wayne Mauthe</name></author><author><name sortKey="Linning, Rob" sort="Linning, Rob" uniqKey="Linning R" first="Rob" last="Linning">Rob Linning</name></author><author><name sortKey="Banks, Travis" sort="Banks, Travis" uniqKey="Banks T" first="Travis" last="Banks">Travis Banks</name></author><author><name sortKey="Mccallum, Brent" sort="Mccallum, Brent" uniqKey="Mccallum B" first="Brent" last="Mccallum">Brent Mccallum</name></author><author><name sortKey="Bakkeren, Guus" sort="Bakkeren, Guus" uniqKey="Bakkeren G" first="Guus" last="Bakkeren">Guus Bakkeren</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (chemistry)</term><term>Basidiomycota (genetics)</term><term>Centrifugation, Density Gradient (MeSH)</term><term>Cluster Analysis (MeSH)</term><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Expressed Sequence Tags (chemistry)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Host-Parasite Interactions (MeSH)</term><term>Mass Spectrometry (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Proteome (genetics)</term><term>Proteome (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Sequence Analysis (MeSH)</term><term>Sequence Homology (MeSH)</term><term>Triticum (microbiology)</term><term>Up-Regulation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Basidiomycota</term><term>Expressed Sequence Tags</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Fungal Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Centrifugation, Density Gradient</term><term>Cluster Analysis</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Gene Expression Regulation, Fungal</term><term>Host-Parasite Interactions</term><term>Mass Spectrometry</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis</term><term>Sequence Homology</term><term>Up-Regulation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Puccinia triticina (Pt) is a representative of several cereal-infecting rust fungal pathogens of major economic importance world wide. Upon entry through leaf stomata, these fungi establish intracellular haustoria, crucial feeding structures. We report the first proteome of infection structures from parasitized wheat leaves, enriched for haustoria through filtration and sucrose density centrifugation. 2-D PAGE MS/MS and gel-based LC-MS (GeLC-MS) were used to separate proteins. Generated spectra were compared with a partial proteome predicted from a preliminary Pt genome and generated ESTs, to a comprehensive genome-predicted protein complement from the related wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt) and to various plant resources. We identified over 260 fungal proteins, 16 of which matched peptides from Pgt. Based on bioinformatic analyses and/or the presence of a signal peptide, at least 50 proteins were predicted to be secreted. Among those, six have effector protein signatures, some are related and the respective genes of several seem to belong to clusters. Many ribosomal structural proteins, proteins involved in energy, general metabolism and transport were detected. Measuring gene expression over several life cycle stages of ten representative candidates using quantitative RT-PCR, all were shown to be strongly upregulated and four expressed solely upon infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21280219</PMID><DateCompleted><Year>2011</Year><Month>08</Month><Day>01</Day></DateCompleted><DateRevised><Year>2011</Year><Month>02</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>5</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Proteome analysis of wheat leaf rust fungus, Puccinia triticina, infection structures enriched for haustoria.</ArticleTitle><Pagination><MedlinePgn>944-63</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.201000014</ELocationID><Abstract><AbstractText>Puccinia triticina (Pt) is a representative of several cereal-infecting rust fungal pathogens of major economic importance world wide. Upon entry through leaf stomata, these fungi establish intracellular haustoria, crucial feeding structures. We report the first proteome of infection structures from parasitized wheat leaves, enriched for haustoria through filtration and sucrose density centrifugation. 2-D PAGE MS/MS and gel-based LC-MS (GeLC-MS) were used to separate proteins. Generated spectra were compared with a partial proteome predicted from a preliminary Pt genome and generated ESTs, to a comprehensive genome-predicted protein complement from the related wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt) and to various plant resources. We identified over 260 fungal proteins, 16 of which matched peptides from Pgt. Based on bioinformatic analyses and/or the presence of a signal peptide, at least 50 proteins were predicted to be secreted. Among those, six have effector protein signatures, some are related and the respective genes of several seem to belong to clusters. Many ribosomal structural proteins, proteins involved in energy, general metabolism and transport were detected. Measuring gene expression over several life cycle stages of ten representative candidates using quantitative RT-PCR, all were shown to be strongly upregulated and four expressed solely upon infection.</AbstractText><CopyrightInformation>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. 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