Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust.
Identifieur interne : 000118 ( Main/Corpus ); précédent : 000117; suivant : 000119Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust.
Auteurs : Letizia Bernardo ; Bhakti Prinsi ; Alfredo Simone Negri ; Luigi Cattivelli ; Luca Espen ; Giampiero ValèSource :
- BMC genomics [ 1471-2164 ] ; 2012.
English descriptors
- KwdEn :
- Basidiomycota (immunology), Blotting, Western (MeSH), Chromatography, Liquid (MeSH), Disease Resistance (genetics), Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Profiling (MeSH), Hordeum (genetics), Hordeum (immunology), Plant Diseases (immunology), Plant Diseases (microbiology), Proteome (MeSH), Proteomics (MeSH), Real-Time Polymerase Chain Reaction (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical : Proteome.
- genetics : Disease Resistance, Hordeum.
- immunology : Basidiomycota, Hordeum, Plant Diseases.
- microbiology : Plant Diseases.
- Blotting, Western, Chromatography, Liquid, Electrophoresis, Gel, Two-Dimensional, Gene Expression Profiling, Proteomics, Real-Time Polymerase Chain Reaction, Tandem Mass Spectrometry.
Abstract
BACKGROUND
Leaf rust, caused by the biotrophic fungal pathogen Puccinia hordei, is one of the most important foliar disease of barley (Hordeum vulgare) and represents a serious threat in many production regions of the world. The leaf rust resistance gene Rph15 is of outstanding interest for resistance breeding because it confers resistance to over 350 Puccinia hordei isolates collected from around the world. Molecular and biochemical mechanisms responsible for the Rph15 effectiveness are currently not investigated. The aim of the present work was to study the Rph15-based defence responses using a proteomic approach.
RESULTS
Protein pattern changes in response to the leaf rust pathogen infection were investigated in two barley near isogenic lines (NILs), Bowman (leaf rust susceptible) and Bowman-Rph15 (leaf rust resistant), differing for the introgression of the leaf rust resistance gene Rph15. Two infection time points, 24 hours and four days post inoculation (dpi), were analysed. No statistically significant differences were identified at the early time point, while at 4 dpi eighteen protein spots were significantly up or down regulated with a fold-change equal or higher than two in response to pathogen infection. Almost all the pathogen-responsive proteins were identified in the Bowman-Rph15 resistant NIL. Protein spots were characterized by LC-MS/MS analysis and found to be involved in photosynthesis and energy metabolism, carbohydrate metabolism, protein degradation and defence. Proteomic data were complemented by transcriptional analysis of the respective genes. The identified proteins can be related to modulation of the photosynthetic apparatus components, re-direction of the metabolism to sustain defence responses and deployment of defence proteins.
CONCLUSIONS
The identification of leaf rust infection-modulated defence responses restricted to the resistant NIL support the hypothesis that basal defence responses of Bowman, but not the Rph15 resistance gene-based ones, are suppressed or delayed by pathogen effectors to levels below the detection power of the adopted proteomic approach. Additionally, Rph15-mediated resistance processes identified mainly resides on a modulation of primary metabolism, affecting photosyntesis and carbohydrate pool.
DOI: 10.1186/1471-2164-13-642
PubMed: 23167439
PubMed Central: PMC3541957
Links to Exploration step
pubmed:23167439Le document en format XML
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Protaso 302, Fiorenzuola d'Arda, PC I-29017, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Prinsi, Bhakti" sort="Prinsi, Bhakti" uniqKey="Prinsi B" first="Bhakti" last="Prinsi">Bhakti Prinsi</name></author><author><name sortKey="Negri, Alfredo Simone" sort="Negri, Alfredo Simone" uniqKey="Negri A" first="Alfredo Simone" last="Negri">Alfredo Simone Negri</name></author><author><name sortKey="Cattivelli, Luigi" sort="Cattivelli, Luigi" uniqKey="Cattivelli L" first="Luigi" last="Cattivelli">Luigi Cattivelli</name></author><author><name sortKey="Espen, Luca" sort="Espen, Luca" uniqKey="Espen L" first="Luca" last="Espen">Luca Espen</name></author><author><name sortKey="Vale, Giampiero" sort="Vale, Giampiero" uniqKey="Vale G" first="Giampiero" last="Valè">Giampiero Valè</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:23167439</idno><idno type="pmid">23167439</idno><idno type="doi">10.1186/1471-2164-13-642</idno><idno type="pmc">PMC3541957</idno><idno type="wicri:Area/Main/Corpus">000118</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000118</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust.</title><author><name sortKey="Bernardo, Letizia" sort="Bernardo, Letizia" uniqKey="Bernardo L" first="Letizia" last="Bernardo">Letizia Bernardo</name><affiliation><nlm:affiliation>CRA-Consiglio per la ricerca e la sperimentazione in agricoltura, Genomics Research Centre, Via S. Protaso 302, Fiorenzuola d'Arda, PC I-29017, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Prinsi, Bhakti" sort="Prinsi, Bhakti" uniqKey="Prinsi B" first="Bhakti" last="Prinsi">Bhakti Prinsi</name></author><author><name sortKey="Negri, Alfredo Simone" sort="Negri, Alfredo Simone" uniqKey="Negri A" first="Alfredo Simone" last="Negri">Alfredo Simone Negri</name></author><author><name sortKey="Cattivelli, Luigi" sort="Cattivelli, Luigi" uniqKey="Cattivelli L" first="Luigi" last="Cattivelli">Luigi Cattivelli</name></author><author><name sortKey="Espen, Luca" sort="Espen, Luca" uniqKey="Espen L" first="Luca" last="Espen">Luca Espen</name></author><author><name sortKey="Vale, Giampiero" sort="Vale, Giampiero" uniqKey="Vale G" first="Giampiero" last="Valè">Giampiero Valè</name></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (immunology)</term><term>Blotting, Western (MeSH)</term><term>Chromatography, Liquid (MeSH)</term><term>Disease Resistance (genetics)</term><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Hordeum (genetics)</term><term>Hordeum (immunology)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Proteome (MeSH)</term><term>Proteomics (MeSH)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Hordeum</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Basidiomycota</term><term>Hordeum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Chromatography, Liquid</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Gene Expression Profiling</term><term>Proteomics</term><term>Real-Time Polymerase Chain Reaction</term><term>Tandem Mass Spectrometry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Leaf rust, caused by the biotrophic fungal pathogen Puccinia hordei, is one of the most important foliar disease of barley (Hordeum vulgare) and represents a serious threat in many production regions of the world. The leaf rust resistance gene Rph15 is of outstanding interest for resistance breeding because it confers resistance to over 350 Puccinia hordei isolates collected from around the world. Molecular and biochemical mechanisms responsible for the Rph15 effectiveness are currently not investigated. The aim of the present work was to study the Rph15-based defence responses using a proteomic approach.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Protein pattern changes in response to the leaf rust pathogen infection were investigated in two barley near isogenic lines (NILs), Bowman (leaf rust susceptible) and Bowman-Rph15 (leaf rust resistant), differing for the introgression of the leaf rust resistance gene Rph15. Two infection time points, 24 hours and four days post inoculation (dpi), were analysed. No statistically significant differences were identified at the early time point, while at 4 dpi eighteen protein spots were significantly up or down regulated with a fold-change equal or higher than two in response to pathogen infection. Almost all the pathogen-responsive proteins were identified in the Bowman-Rph15 resistant NIL. Protein spots were characterized by LC-MS/MS analysis and found to be involved in photosynthesis and energy metabolism, carbohydrate metabolism, protein degradation and defence. Proteomic data were complemented by transcriptional analysis of the respective genes. The identified proteins can be related to modulation of the photosynthetic apparatus components, re-direction of the metabolism to sustain defence responses and deployment of defence proteins.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The identification of leaf rust infection-modulated defence responses restricted to the resistant NIL support the hypothesis that basal defence responses of Bowman, but not the Rph15 resistance gene-based ones, are suppressed or delayed by pathogen effectors to levels below the detection power of the adopted proteomic approach. Additionally, Rph15-mediated resistance processes identified mainly resides on a modulation of primary metabolism, affecting photosyntesis and carbohydrate pool.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23167439</PMID><DateCompleted><Year>2013</Year><Month>06</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><PubDate><Year>2012</Year><Month>Nov</Month><Day>20</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust.</ArticleTitle><Pagination><MedlinePgn>642</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1471-2164-13-642</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Leaf rust, caused by the biotrophic fungal pathogen Puccinia hordei, is one of the most important foliar disease of barley (Hordeum vulgare) and represents a serious threat in many production regions of the world. The leaf rust resistance gene Rph15 is of outstanding interest for resistance breeding because it confers resistance to over 350 Puccinia hordei isolates collected from around the world. Molecular and biochemical mechanisms responsible for the Rph15 effectiveness are currently not investigated. The aim of the present work was to study the Rph15-based defence responses using a proteomic approach.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Protein pattern changes in response to the leaf rust pathogen infection were investigated in two barley near isogenic lines (NILs), Bowman (leaf rust susceptible) and Bowman-Rph15 (leaf rust resistant), differing for the introgression of the leaf rust resistance gene Rph15. Two infection time points, 24 hours and four days post inoculation (dpi), were analysed. No statistically significant differences were identified at the early time point, while at 4 dpi eighteen protein spots were significantly up or down regulated with a fold-change equal or higher than two in response to pathogen infection. Almost all the pathogen-responsive proteins were identified in the Bowman-Rph15 resistant NIL. Protein spots were characterized by LC-MS/MS analysis and found to be involved in photosynthesis and energy metabolism, carbohydrate metabolism, protein degradation and defence. Proteomic data were complemented by transcriptional analysis of the respective genes. The identified proteins can be related to modulation of the photosynthetic apparatus components, re-direction of the metabolism to sustain defence responses and deployment of defence proteins.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The identification of leaf rust infection-modulated defence responses restricted to the resistant NIL support the hypothesis that basal defence responses of Bowman, but not the Rph15 resistance gene-based ones, are suppressed or delayed by pathogen effectors to levels below the detection power of the adopted proteomic approach. 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