Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi.
Identifieur interne : 000090 ( Main/Corpus ); précédent : 000089; suivant : 000091Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi.
Auteurs : Alice Casassola ; Sandra P. Brammer ; Márcia S. Chaves ; José A. Martinelli ; Francesca Stefanato ; Lesley A. BoydSource :
- Physiological and molecular plant pathology [ 0885-5765 ] ; 2015.
Abstract
Leaf rust, caused by the foliar pathogen Puccinia triticina is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between P. triticina and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72 h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding.
DOI: 10.1016/j.pmpp.2014.12.004
PubMed: 25892845
PubMed Central: PMC4394150
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Brammer</name><affiliation><nlm:affiliation>Department of Biotechnology and Phytophatology, Brazilian Agricultural Research Corporation, BR285, Km294, Passo Fundo, Rio Grande do Sul 99001970, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Chaves, Marcia S" sort="Chaves, Marcia S" uniqKey="Chaves M" first="Márcia S" last="Chaves">Márcia S. Chaves</name><affiliation><nlm:affiliation>Department of Biotechnology and Phytophatology, Brazilian Agricultural Research Corporation, BR285, Km294, Passo Fundo, Rio Grande do Sul 99001970, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Martinelli, Jose A" sort="Martinelli, Jose A" uniqKey="Martinelli J" first="José A" last="Martinelli">José A. Martinelli</name><affiliation><nlm:affiliation>Department of Fitossanidade, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves 7712, Porto Alegre, Rio Grande do Sul 91540000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Stefanato, Francesca" sort="Stefanato, Francesca" uniqKey="Stefanato F" first="Francesca" last="Stefanato">Francesca Stefanato</name><affiliation><nlm:affiliation>Department of Genetics and Pre-Breeding, National Institute of Agricultural Botany, Huntington Road, Cambridge, Cambridgeshire CB3 0LE, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Boyd, Lesley A" sort="Boyd, Lesley A" uniqKey="Boyd L" first="Lesley A" last="Boyd">Lesley A. Boyd</name><affiliation><nlm:affiliation>Department of Genetics and Pre-Breeding, National Institute of Agricultural Botany, Huntington Road, Cambridge, Cambridgeshire CB3 0LE, UK ; Department of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25892845</idno><idno type="pmid">25892845</idno><idno type="doi">10.1016/j.pmpp.2014.12.004</idno><idno type="pmc">PMC4394150</idno><idno type="wicri:Area/Main/Corpus">000090</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000090</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi.</title><author><name sortKey="Casassola, Alice" sort="Casassola, Alice" uniqKey="Casassola A" first="Alice" last="Casassola">Alice Casassola</name><affiliation><nlm:affiliation>Agronomy Post-Graduate Program, University of Passo Fundo, BR285, Passo Fundo, Rio Grande do Sul 99052900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Brammer, Sandra P" sort="Brammer, Sandra P" uniqKey="Brammer S" first="Sandra P" last="Brammer">Sandra P. Brammer</name><affiliation><nlm:affiliation>Department of Biotechnology and Phytophatology, Brazilian Agricultural Research Corporation, BR285, Km294, Passo Fundo, Rio Grande do Sul 99001970, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Chaves, Marcia S" sort="Chaves, Marcia S" uniqKey="Chaves M" first="Márcia S" last="Chaves">Márcia S. Chaves</name><affiliation><nlm:affiliation>Department of Biotechnology and Phytophatology, Brazilian Agricultural Research Corporation, BR285, Km294, Passo Fundo, Rio Grande do Sul 99001970, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Martinelli, Jose A" sort="Martinelli, Jose A" uniqKey="Martinelli J" first="José A" last="Martinelli">José A. Martinelli</name><affiliation><nlm:affiliation>Department of Fitossanidade, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves 7712, Porto Alegre, Rio Grande do Sul 91540000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Stefanato, Francesca" sort="Stefanato, Francesca" uniqKey="Stefanato F" first="Francesca" last="Stefanato">Francesca Stefanato</name><affiliation><nlm:affiliation>Department of Genetics and Pre-Breeding, National Institute of Agricultural Botany, Huntington Road, Cambridge, Cambridgeshire CB3 0LE, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Boyd, Lesley A" sort="Boyd, Lesley A" uniqKey="Boyd L" first="Lesley A" last="Boyd">Lesley A. Boyd</name><affiliation><nlm:affiliation>Department of Genetics and Pre-Breeding, National Institute of Agricultural Botany, Huntington Road, Cambridge, Cambridgeshire CB3 0LE, UK ; Department of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Physiological and molecular plant pathology</title><idno type="ISSN">0885-5765</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Leaf rust, caused by the foliar pathogen <i>Puccinia triticina</i> is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between <i>P. triticina</i> and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72 h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">25892845</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0885-5765</ISSN><JournalIssue CitedMedium="Print"><Volume>89</Volume><PubDate><Year>2015</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Physiological and molecular plant pathology</Title><ISOAbbreviation>Physiol Mol Plant Pathol</ISOAbbreviation></Journal><ArticleTitle>Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi.</ArticleTitle><Pagination><MedlinePgn>49-54</MedlinePgn></Pagination><Abstract><AbstractText>Leaf rust, caused by the foliar pathogen <i>Puccinia triticina</i> is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between <i>P. triticina</i> and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72 h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Casassola</LastName><ForeName>Alice</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Agronomy Post-Graduate Program, University of Passo Fundo, BR285, Passo Fundo, Rio Grande do Sul 99052900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brammer</LastName><ForeName>Sandra P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Department of Biotechnology and Phytophatology, Brazilian Agricultural Research Corporation, BR285, Km294, Passo Fundo, Rio Grande do Sul 99001970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chaves</LastName><ForeName>Márcia S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Biotechnology and Phytophatology, Brazilian Agricultural Research Corporation, BR285, Km294, Passo Fundo, Rio Grande do Sul 99001970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martinelli</LastName><ForeName>José A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Department of Fitossanidade, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves 7712, Porto Alegre, Rio Grande do Sul 91540000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stefanato</LastName><ForeName>Francesca</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Genetics and Pre-Breeding, National Institute of Agricultural Botany, Huntington Road, Cambridge, Cambridgeshire CB3 0LE, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boyd</LastName><ForeName>Lesley A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Department of Genetics and Pre-Breeding, National Institute of Agricultural Botany, Huntington Road, Cambridge, Cambridgeshire CB3 0LE, UK ; Department of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Physiol Mol Plant Pathol</MedlineTA><NlmUniqueID>9882868</NlmUniqueID><ISSNLinking>0885-5765</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">APR, adult plant resistance</Keyword><Keyword MajorTopicYN="N">AQP1, aquaporin</Keyword><Keyword MajorTopicYN="N">COMT1, caffeic acid O-methyltransferase</Keyword><Keyword MajorTopicYN="N">ETI, Effector-Triggered-Immunity</Keyword><Keyword MajorTopicYN="N">FREX, fructan exohydrolase</Keyword><Keyword MajorTopicYN="N">G6DPH, glucose-6-phosphate dehydrogenase</Keyword><Keyword MajorTopicYN="N">GAPDH, glyceraldehyde 3-phosphate dehydrogenase</Keyword><Keyword MajorTopicYN="N">HSP80, heat shock protein 80</Keyword><Keyword MajorTopicYN="N">LHC, light-harvesting complex</Keyword><Keyword MajorTopicYN="N">LTP, type 1 non-specific lipid transfer protein precursor</Keyword><Keyword MajorTopicYN="N">MIP, major intrinsic proteins</Keyword><Keyword MajorTopicYN="N">NADPH, nicotinamide adenine dinucleotide phosphate</Keyword><Keyword MajorTopicYN="N">PAL, phenylalanine ammonia-lyase</Keyword><Keyword MajorTopicYN="N">PR, pathogenesis-related</Keyword><Keyword MajorTopicYN="N">PRA2, class III peroxidase</Keyword><Keyword MajorTopicYN="N">PTI, PAMP-Triggered-Immunity</Keyword><Keyword MajorTopicYN="N">Pre-haustorial</Keyword><Keyword MajorTopicYN="N">Puccinia triticina</Keyword><Keyword MajorTopicYN="N">Quantitative PCR</Keyword><Keyword MajorTopicYN="N">RBR1, retinoblastoma related protein 1</Keyword><Keyword MajorTopicYN="N">ROS, reactive oxygen species</Keyword><Keyword MajorTopicYN="N">Triticum aestivum (L.) thell</Keyword><Keyword MajorTopicYN="N">WCAB, chlorophyll a/b-binding protein WCAB precursor</Keyword><Keyword MajorTopicYN="N">Wheat breeding</Keyword><Keyword MajorTopicYN="N">ZIP5, putative zinc transporter</Keyword><Keyword MajorTopicYN="N">hai, hours after inoculation</Keyword><Keyword MajorTopicYN="N">qPCR, quantitative PCR</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>12</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25892845</ArticleId><ArticleId IdType="doi">10.1016/j.pmpp.2014.12.004</ArticleId><ArticleId IdType="pii">S0885-5765(14)00086-1</ArticleId><ArticleId IdType="pmc">PMC4394150</ArticleId></ArticleIdList><pmc-dir>pmcsd</pmc-dir>
<ReferenceList><Reference><Citation>Hereditas. 2001;135(2-3):111-4</Citation><ArticleIdList><ArticleId IdType="pubmed">12152322</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2012 Aug;63(13):4647-70</Citation><ArticleIdList><ArticleId IdType="pubmed">22732107</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2008 Aug 15;414(1):53-61</Citation><ArticleIdList><ArticleId IdType="pubmed">18462192</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Dec;200(4):1187-99</Citation><ArticleIdList><ArticleId IdType="pubmed">23952213</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2008 Mar;9(2):137-45</Citation><ArticleIdList><ArticleId IdType="pubmed">18705847</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2002 Jun 18;3(7):RESEARCH0034</Citation><ArticleIdList><ArticleId IdType="pubmed">12184808</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2006;57(1):213-23</Citation><ArticleIdList><ArticleId IdType="pubmed">16330524</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2006;44:135-62</Citation><ArticleIdList><ArticleId IdType="pubmed">16602946</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2012 Jan;1817(1):218-31</Citation><ArticleIdList><ArticleId IdType="pubmed">21641332</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2004 Nov;9(11):534-40</Citation><ArticleIdList><ArticleId IdType="pubmed">15501178</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:627-654</Citation><ArticleIdList><ArticleId IdType="pubmed">15012303</ArticleId></ArticleIdList></Reference><Reference><Citation>J Environ Sci (China). 2012;24(10):1843-53</Citation><ArticleIdList><ArticleId IdType="pubmed">23520855</ArticleId></ArticleIdList></Reference><Reference><Citation>Genet Mol Res. 2012 Aug 24;11(3):2823-34</Citation><ArticleIdList><ArticleId IdType="pubmed">23007977</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2005 Aug 15;1730(2):114-25</Citation><ArticleIdList><ArticleId IdType="pubmed">16061294</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2004 Nov;15(11):5118-29</Citation><ArticleIdList><ArticleId IdType="pubmed">15342780</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2010 Oct;22(10):3357-73</Citation><ArticleIdList><ArticleId IdType="pubmed">20952635</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiology. 2002 Nov;148(Pt 11):3705-13</Citation><ArticleIdList><ArticleId IdType="pubmed">12427960</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2002 Oct;130(2):549-60</Citation><ArticleIdList><ArticleId IdType="pubmed">12376624</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Sep 17;8(9):e75293</Citation><ArticleIdList><ArticleId IdType="pubmed">24069397</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(12):e52439</Citation><ArticleIdList><ArticleId IdType="pubmed">23285044</ArticleId></ArticleIdList></Reference><Reference><Citation>J Plant Physiol. 2011 Jun 15;168(9):990-4</Citation><ArticleIdList><ArticleId IdType="pubmed">21315476</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2008 May 01;9:203</Citation><ArticleIdList><ArticleId IdType="pubmed">18447959</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2012 Dec;53(12):2127-41</Citation><ArticleIdList><ArticleId IdType="pubmed">23161856</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Oct 30;425(6961):973-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14586469</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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