Reference Genes in the Pathosystem Phakopsora pachyrhizi/ Soybean Suitable for Normalization in Transcript Profiling.
Identifieur interne : 000088 ( Main/Corpus ); précédent : 000087; suivant : 000089Reference Genes in the Pathosystem Phakopsora pachyrhizi/ Soybean Suitable for Normalization in Transcript Profiling.
Auteurs : Daniela Hirschburger ; Manuel Müller ; Ralf T. Voegele ; Tobias LinkSource :
- International journal of molecular sciences [ 1422-0067 ] ; 2015.
English descriptors
- KwdEn :
- Gene Expression Regulation, Fungal (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Essential (MeSH), Host-Pathogen Interactions (MeSH), Phakopsora pachyrhizi (genetics), Phakopsora pachyrhizi (physiology), Plant Diseases (genetics), Plant Diseases (microbiology), Real-Time Polymerase Chain Reaction (MeSH), Soybeans (genetics), Soybeans (microbiology), Transcriptome (MeSH).
- MESH :
- genetics : Phakopsora pachyrhizi, Plant Diseases, Soybeans.
- microbiology : Plant Diseases, Soybeans.
- physiology : Phakopsora pachyrhizi.
- Gene Expression Regulation, Fungal, Gene Expression Regulation, Plant, Genes, Essential, Host-Pathogen Interactions, Real-Time Polymerase Chain Reaction, Transcriptome.
Abstract
Phakopsora pachyrhizi is a devastating pathogen on soybean, endangering soybean production worldwide. Use of Host Induced Gene Silencing (HIGS) and the study of effector proteins could provide novel strategies for pathogen control. For both approaches quantification of transcript abundance by RT-qPCR is essential. Suitable stable reference genes for normalization are indispensable to obtain accurate RT-qPCR results. According to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines and using algorithms geNorm and NormFinder we tested candidate reference genes from P. pachyrhizi and Glycine max for their suitability in normalization of transcript levels throughout the infection process. For P. pachyrhizi we recommend a combination of CytB and PDK or GAPDH for in planta experiments. Gene expression during in vitro stages and over the whole infection process was found to be highly unstable. Here, RPS14 and UbcE2 are ranked best by geNorm and NormFinder. Alternatively CytB that has the smallest Cq range (Cq: quantification cycle) could be used. We recommend specification of gene expression relative to the germ tube stage rather than to the resting urediospore stage. For studies omitting the resting spore and the appressorium stages a combination of Elf3 and RPS9, or PKD and GAPDH should be used. For normalization of soybean genes during rust infection Ukn2 and cons7 are recommended.
DOI: 10.3390/ijms160923057
PubMed: 26404265
PubMed Central: PMC4613351
Links to Exploration step
pubmed:26404265Le document en format XML
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<term>Phakopsora pachyrhizi (genetics)</term>
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<front><div type="abstract" xml:lang="en">Phakopsora pachyrhizi is a devastating pathogen on soybean, endangering soybean production worldwide. Use of Host Induced Gene Silencing (HIGS) and the study of effector proteins could provide novel strategies for pathogen control. For both approaches quantification of transcript abundance by RT-qPCR is essential. Suitable stable reference genes for normalization are indispensable to obtain accurate RT-qPCR results. According to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines and using algorithms geNorm and NormFinder we tested candidate reference genes from P. pachyrhizi and Glycine max for their suitability in normalization of transcript levels throughout the infection process. For P. pachyrhizi we recommend a combination of CytB and PDK or GAPDH for in planta experiments. Gene expression during in vitro stages and over the whole infection process was found to be highly unstable. Here, RPS14 and UbcE2 are ranked best by geNorm and NormFinder. Alternatively CytB that has the smallest Cq range (Cq: quantification cycle) could be used. We recommend specification of gene expression relative to the germ tube stage rather than to the resting urediospore stage. For studies omitting the resting spore and the appressorium stages a combination of Elf3 and RPS9, or PKD and GAPDH should be used. For normalization of soybean genes during rust infection Ukn2 and cons7 are recommended. </div>
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<ReferenceList><Reference><Citation>Mol Plant Pathol. 2014 May;15(4):379-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24341524</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(9):e46487</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23029532</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2008 Jan;9(1):59-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18705884</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2009;183(4):993-1000</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19558422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Mol Biol. 2009;10:93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19785741</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pest Manag Sci. 2014 Mar;70(3):378-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23589453</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 May;153(1):52-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20200069</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 1997 May;10(4):427-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9150592</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2002 Jun 18;3(7):RESEARCH0034</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12184808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2014 Aug 20;5:416</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25191335</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2007 May 15;23(10):1289-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17379693</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Lett. 2011 Sep;322(2):131-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21707731</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2009;4(7):e6162</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19584937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2010 Sep;22(9):3130-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20884801</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Biol. 2011 Sep;115(9):891-901</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21872186</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2011 Mar;11(5):944-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21280219</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2001 May 1;29(9):e45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11328886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2009 Apr;55(4):611-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19246619</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013;8(10):e75271</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24124481</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2009 Feb;22(2):123-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19132865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2010 Jan;61(2):364-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19874543</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Mol Biol. 2008;9:59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18573215</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2015;10(8):e0134890</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26244340</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 May 31;108(22):9166-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21536894</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Aug;40(15):e115</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22730293</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2010 Mar;11(2):169-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20447267</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Res. 2004 Aug 1;64(15):5245-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15289330</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2005 Dec;42(12):949-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16291502</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(12):e15208</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21179435</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2007 Aug;20(8):887-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17722693</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Res Notes. 2013;6:196</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23668315</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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