Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies.
Identifieur interne : 000081 ( Main/Corpus ); précédent : 000080; suivant : 000082Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies.
Auteurs : Benjamin Petre ; Diane G O. Saunders ; Jan Sklenar ; Cécile Lorrain ; Ksenia V. Krasileva ; Joe Win ; Sébastien Duplessis ; Sophien KamounSource :
- PloS one [ 1932-6203 ] ; 2016.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (metabolism), Basidiomycota (pathogenicity), Chromatography, Liquid (MeSH), Computer Simulation (MeSH), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Green Fluorescent Proteins (metabolism), Microscopy, Confocal (MeSH), Open Reading Frames (MeSH), Plant Diseases (microbiology), Plant Leaves (metabolism), Plant Leaves (microbiology), Protein Interaction Mapping (MeSH), RNA, Messenger (metabolism), Tandem Mass Spectrometry (MeSH), Tobacco (microbiology), Triticum (microbiology), Virulence (MeSH).
- MESH :
- chemical , metabolism : Fungal Proteins, Green Fluorescent Proteins, RNA, Messenger.
- genetics : Basidiomycota.
- metabolism : Basidiomycota, Plant Leaves.
- microbiology : Plant Diseases, Plant Leaves, Tobacco, Triticum.
- pathogenicity : Basidiomycota.
- Chromatography, Liquid, Computer Simulation, Gene Expression Profiling, Gene Expression Regulation, Plant, Microscopy, Confocal, Open Reading Frames, Protein Interaction Mapping, Tandem Mass Spectrometry, Virulence.
Abstract
Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors.
DOI: 10.1371/journal.pone.0149035
PubMed: 26863009
PubMed Central: PMC4749346
Links to Exploration step
pubmed:26863009Le document en format XML
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<affiliation><nlm:affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.</nlm:affiliation>
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<author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name>
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<term>Computer Simulation (MeSH)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
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<term>Plant Leaves (metabolism)</term>
<term>Plant Leaves (microbiology)</term>
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<term>Tobacco (microbiology)</term>
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<front><div type="abstract" xml:lang="en">Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors. </div>
</front>
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<Abstract><AbstractText>Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors. </AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petre</LastName>
<ForeName>Benjamin</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.</Affiliation>
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<AffiliationInfo><Affiliation>INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.</Affiliation>
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</AffiliationInfo>
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