The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.
Identifieur interne : 000076 ( Main/Corpus ); précédent : 000075; suivant : 000077The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.
Auteurs : Changhai Liu ; Carsten Pedersen ; Torsten Schultz-Larsen ; Geziel B. Aguilar ; Kenneth Madriz-Orde Ana ; Mogens S. Hovm Ller ; Hans Thordal-ChristensenSource :
- The New phytologist [ 1469-8137 ] ; 2016.
Abstract
We identified a wheat stripe rust (Puccinia striiformis) effector candidate (PEC6) with pattern-triggered immunity (PTI) suppression function and its corresponding host target. PEC6 compromised PTI host species-independently. In Nicotiana benthamiana, it hampers reactive oxygen species (ROS) accumulation and callose deposition induced by Pseudomonas fluorescens. In Arabidopsis, plants expressing PEC6 were more susceptible to Pseudomonas syringae pv. tomato (Pto) DC3000 ΔAvrPto/ΔAvrPtoB. In wheat, PEC6-suppression of P. fluorescens-elicited PTI was revealed by the fact that it allowed activation of effector-triggered immunity by Pto DC3000. Knocking down of PEC6 expression by virus-mediated host-induced gene silencing decreased the number of rust pustules, uncovering PEC6 as an important pathogenicity factor. PEC6, overexpressed in plant cells without its signal peptide, was localized to the nucleus and cytoplasm. A yeast two-hybrid assay showed that PEC6 interacts with both wheat and Arabidopsis adenosine kinases (ADKs). Knocking down wheat ADK expression by virus-induced gene silencing reduced leaf growth and enhanced the number of rust pustules, indicating that ADK is important in plant development and defence. ADK plays essential roles in regulating metabolism, cytokinin interconversion and methyl transfer reactions, and our data propose a model where PEC6 may affect one of these processes by targeting ADK to favour fungal growth.
DOI: 10.1111/nph.14034
PubMed: 27252028
Links to Exploration step
pubmed:27252028Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.</title><author><name sortKey="Liu, Changhai" sort="Liu, Changhai" uniqKey="Liu C" first="Changhai" last="Liu">Changhai Liu</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Pedersen, Carsten" sort="Pedersen, Carsten" uniqKey="Pedersen C" first="Carsten" last="Pedersen">Carsten Pedersen</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Schultz Larsen, Torsten" sort="Schultz Larsen, Torsten" uniqKey="Schultz Larsen T" first="Torsten" last="Schultz-Larsen">Torsten Schultz-Larsen</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Aguilar, Geziel B" sort="Aguilar, Geziel B" uniqKey="Aguilar G" first="Geziel B" last="Aguilar">Geziel B. Aguilar</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Madriz Orde Ana, Kenneth" sort="Madriz Orde Ana, Kenneth" uniqKey="Madriz Orde Ana K" first="Kenneth" last="Madriz-Orde Ana">Kenneth Madriz-Orde Ana</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Hovm Ller, Mogens S" sort="Hovm Ller, Mogens S" uniqKey="Hovm Ller M" first="Mogens S" last="Hovm Ller">Mogens S. Hovm Ller</name><affiliation><nlm:affiliation>Department of Agroecology, Aarhus University, Forsøgsvej 1, Slagelse, DK-4200, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Thordal Christensen, Hans" sort="Thordal Christensen, Hans" uniqKey="Thordal Christensen H" first="Hans" last="Thordal-Christensen">Hans Thordal-Christensen</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27252028</idno><idno type="pmid">27252028</idno><idno type="doi">10.1111/nph.14034</idno><idno type="wicri:Area/Main/Corpus">000076</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000076</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.</title><author><name sortKey="Liu, Changhai" sort="Liu, Changhai" uniqKey="Liu C" first="Changhai" last="Liu">Changhai Liu</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Pedersen, Carsten" sort="Pedersen, Carsten" uniqKey="Pedersen C" first="Carsten" last="Pedersen">Carsten Pedersen</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Schultz Larsen, Torsten" sort="Schultz Larsen, Torsten" uniqKey="Schultz Larsen T" first="Torsten" last="Schultz-Larsen">Torsten Schultz-Larsen</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Aguilar, Geziel B" sort="Aguilar, Geziel B" uniqKey="Aguilar G" first="Geziel B" last="Aguilar">Geziel B. Aguilar</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Madriz Orde Ana, Kenneth" sort="Madriz Orde Ana, Kenneth" uniqKey="Madriz Orde Ana K" first="Kenneth" last="Madriz-Orde Ana">Kenneth Madriz-Orde Ana</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Hovm Ller, Mogens S" sort="Hovm Ller, Mogens S" uniqKey="Hovm Ller M" first="Mogens S" last="Hovm Ller">Mogens S. Hovm Ller</name><affiliation><nlm:affiliation>Department of Agroecology, Aarhus University, Forsøgsvej 1, Slagelse, DK-4200, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Thordal Christensen, Hans" sort="Thordal Christensen, Hans" uniqKey="Thordal Christensen H" first="Hans" last="Thordal-Christensen">Hans Thordal-Christensen</name><affiliation><nlm:affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We identified a wheat stripe rust (Puccinia striiformis) effector candidate (PEC6) with pattern-triggered immunity (PTI) suppression function and its corresponding host target. PEC6 compromised PTI host species-independently. In Nicotiana benthamiana, it hampers reactive oxygen species (ROS) accumulation and callose deposition induced by Pseudomonas fluorescens. In Arabidopsis, plants expressing PEC6 were more susceptible to Pseudomonas syringae pv. tomato (Pto) DC3000 ΔAvrPto/ΔAvrPtoB. In wheat, PEC6-suppression of P. fluorescens-elicited PTI was revealed by the fact that it allowed activation of effector-triggered immunity by Pto DC3000. Knocking down of PEC6 expression by virus-mediated host-induced gene silencing decreased the number of rust pustules, uncovering PEC6 as an important pathogenicity factor. PEC6, overexpressed in plant cells without its signal peptide, was localized to the nucleus and cytoplasm. A yeast two-hybrid assay showed that PEC6 interacts with both wheat and Arabidopsis adenosine kinases (ADKs). Knocking down wheat ADK expression by virus-induced gene silencing reduced leaf growth and enhanced the number of rust pustules, indicating that ADK is important in plant development and defence. ADK plays essential roles in regulating metabolism, cytokinin interconversion and methyl transfer reactions, and our data propose a model where PEC6 may affect one of these processes by targeting ADK to favour fungal growth.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">27252028</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2016</Year><Month>06</Month><Day>02</Day></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.14034</ELocationID><Abstract><AbstractText>We identified a wheat stripe rust (Puccinia striiformis) effector candidate (PEC6) with pattern-triggered immunity (PTI) suppression function and its corresponding host target. PEC6 compromised PTI host species-independently. In Nicotiana benthamiana, it hampers reactive oxygen species (ROS) accumulation and callose deposition induced by Pseudomonas fluorescens. In Arabidopsis, plants expressing PEC6 were more susceptible to Pseudomonas syringae pv. tomato (Pto) DC3000 ΔAvrPto/ΔAvrPtoB. In wheat, PEC6-suppression of P. fluorescens-elicited PTI was revealed by the fact that it allowed activation of effector-triggered immunity by Pto DC3000. Knocking down of PEC6 expression by virus-mediated host-induced gene silencing decreased the number of rust pustules, uncovering PEC6 as an important pathogenicity factor. PEC6, overexpressed in plant cells without its signal peptide, was localized to the nucleus and cytoplasm. A yeast two-hybrid assay showed that PEC6 interacts with both wheat and Arabidopsis adenosine kinases (ADKs). Knocking down wheat ADK expression by virus-induced gene silencing reduced leaf growth and enhanced the number of rust pustules, indicating that ADK is important in plant development and defence. ADK plays essential roles in regulating metabolism, cytokinin interconversion and methyl transfer reactions, and our data propose a model where PEC6 may affect one of these processes by targeting ADK to favour fungal growth.</AbstractText><CopyrightInformation>© 2016 University of Copenhagen. New Phytologist © 2016 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Changhai</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pedersen</LastName><ForeName>Carsten</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schultz-Larsen</LastName><ForeName>Torsten</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aguilar</LastName><ForeName>Geziel B</ForeName><Initials>GB</Initials><AffiliationInfo><Affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Madriz-Ordeñana</LastName><ForeName>Kenneth</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hovmøller</LastName><ForeName>Mogens S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Agroecology, Aarhus University, Forsøgsvej 1, Slagelse, DK-4200, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thordal-Christensen</LastName><ForeName>Hans</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Section for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, DK-1871, Denmark.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><CommentsCorrectionsList><CommentsCorrections RefType="ExpressionOfConcernIn"><RefSource>New Phytol. 2017 Feb;213(3):1556</RefSource><PMID Version="1">28079930</PMID></CommentsCorrections></CommentsCorrectionsList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">adenosine kinase</Keyword><Keyword MajorTopicYN="Y">effector</Keyword><Keyword MajorTopicYN="Y">pattern-triggered immunity</Keyword><Keyword MajorTopicYN="Y">stripe rust</Keyword><Keyword MajorTopicYN="Y">wheat (Triticum aestivum)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>6</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>6</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>6</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27252028</ArticleId><ArticleId IdType="doi">10.1111/nph.14034</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RustEffectorV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000076 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000076 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RustEffectorV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:27252028
|texte= The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:27252028" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a RustEffectorV1
| This area was generated with Dilib version V0.6.37. |  |