Primary multiplication site of the vaccinia-rabies glycoprotein recombinant virus administered to foxes by the oral route.
Identifieur interne : 000487 ( PubMed/Corpus ); précédent : 000486; suivant : 000488Primary multiplication site of the vaccinia-rabies glycoprotein recombinant virus administered to foxes by the oral route.
Auteurs : I. Thomas ; B. Brochier ; B. Languet ; J. Blancou ; D. Peharpre ; M P Kieny ; P. Desmettre ; G. Chappuis ; P P PastoretSource :
- The Journal of general virology [ 0022-1317 ] ; 1990.
English descriptors
- KwdEn :
- Administration, Oral, Animals, Base Sequence, DNA, Viral (analysis), DNA, Viral (genetics), Fluorescent Antibody Technique, Foxes, Molecular Sequence Data, Mouth Mucosa (microbiology), Oligonucleotide Probes, Palate, Soft (microbiology), Palatine Tonsil (microbiology), Polymerase Chain Reaction, Rabies (microbiology), Rabies (prevention & control), Rabies (veterinary), Rabies Vaccines (administration & dosage), Rabies virus (genetics), Rabies virus (growth & development), Rabies virus (immunology), Vaccines, Synthetic (administration & dosage), Vaccinia virus (genetics), Vaccinia virus (growth & development), Vaccinia virus (immunology).
- MESH :
- chemical , administration & dosage : Rabies Vaccines, Vaccines, Synthetic.
- chemical , analysis : DNA, Viral.
- chemical , genetics : DNA, Viral.
- genetics : Rabies virus, Vaccinia virus.
- growth & development : Rabies virus, Vaccinia virus.
- immunology : Rabies virus, Vaccinia virus.
- microbiology : Mouth Mucosa, Palate, Soft, Palatine Tonsil, Rabies.
- prevention & control : Rabies.
- veterinary : Rabies.
- Administration, Oral, Animals, Base Sequence, Fluorescent Antibody Technique, Foxes, Molecular Sequence Data, Oligonucleotide Probes, Polymerase Chain Reaction.
Abstract
The primary multiplication site of VVTGgRAB, a recombinant vaccinia virus (VV) expressing the rabies virus G glycoprotein, was studied in comparison with that of the parental VV Copenhagen strain, after oral administration to foxes. Foxes were fed with 10(8) TCID50 of either VVTGgRAB or VV and were sacrificed 12, 24, 48 or 96 h after inoculation. Both viruses were detected by viral isolation in the tonsils during the first 48 h after inoculation at titres between 10(2) and 10(4.3) TCID50/ml. Indirect immunofluorescence confirmed the presence of the virus in tonsils of some of the foxes. The polymerase chain reaction allowed the detection of VVTGgRAB in the tonsils of both of two foxes tested after 24 h, three of three foxes after 48 h, in the buccal mucosa of one of two foxes tested after 24 h and two of three foxes after 48 h and in the soft palate of one of two foxes tested after 24 h and one of three foxes after 48 h. VV was detected in the tonsils of one fox tested after 48 h, in the buccal mucosa of another fox tested after 24 h, and in the first fox after 48 h by the same reaction. Foxes were inoculated with virus isolated from fox tonsils 24 h after oral administration (with or without cell culture amplification) to perform back passages. No virus could be isolated in either case after this passage. The innocuity of VVTGgRAB was also demonstrated when foxes were inoculated with passaged virus.
DOI: 10.1099/0022-1317-71-1-37
PubMed: 2406370
Links to Exploration step
pubmed:2406370Le document en format XML
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Peharpre</name></author><author><name sortKey="Kieny, M P" sort="Kieny, M P" uniqKey="Kieny M" first="M P" last="Kieny">M P Kieny</name></author><author><name sortKey="Desmettre, P" sort="Desmettre, P" uniqKey="Desmettre P" first="P" last="Desmettre">P. Desmettre</name></author><author><name sortKey="Chappuis, G" sort="Chappuis, G" uniqKey="Chappuis G" first="G" last="Chappuis">G. 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Thomas</name><affiliation><nlm:affiliation>Department of Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.</nlm:affiliation></affiliation></author><author><name sortKey="Brochier, B" sort="Brochier, B" uniqKey="Brochier B" first="B" last="Brochier">B. Brochier</name></author><author><name sortKey="Languet, B" sort="Languet, B" uniqKey="Languet B" first="B" last="Languet">B. Languet</name></author><author><name sortKey="Blancou, J" sort="Blancou, J" uniqKey="Blancou J" first="J" last="Blancou">J. Blancou</name></author><author><name sortKey="Peharpre, D" sort="Peharpre, D" uniqKey="Peharpre D" first="D" last="Peharpre">D. Peharpre</name></author><author><name sortKey="Kieny, M P" sort="Kieny, M P" uniqKey="Kieny M" first="M P" last="Kieny">M P Kieny</name></author><author><name sortKey="Desmettre, P" sort="Desmettre, P" uniqKey="Desmettre P" first="P" last="Desmettre">P. Desmettre</name></author><author><name sortKey="Chappuis, G" sort="Chappuis, G" uniqKey="Chappuis G" first="G" last="Chappuis">G. Chappuis</name></author><author><name sortKey="Pastoret, P P" sort="Pastoret, P P" uniqKey="Pastoret P" first="P P" last="Pastoret">P P Pastoret</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Oral</term><term>Animals</term><term>Base Sequence</term><term>DNA, Viral (analysis)</term><term>DNA, Viral (genetics)</term><term>Fluorescent Antibody Technique</term><term>Foxes</term><term>Molecular Sequence Data</term><term>Mouth Mucosa (microbiology)</term><term>Oligonucleotide Probes</term><term>Palate, Soft (microbiology)</term><term>Palatine Tonsil (microbiology)</term><term>Polymerase Chain Reaction</term><term>Rabies (microbiology)</term><term>Rabies (prevention & control)</term><term>Rabies (veterinary)</term><term>Rabies Vaccines (administration & dosage)</term><term>Rabies virus (genetics)</term><term>Rabies virus (growth & development)</term><term>Rabies virus (immunology)</term><term>Vaccines, Synthetic (administration & dosage)</term><term>Vaccinia virus (genetics)</term><term>Vaccinia virus (growth & development)</term><term>Vaccinia virus (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Rabies Vaccines</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rabies virus</term><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Rabies virus</term><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Rabies virus</term><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Mouth Mucosa</term><term>Palate, Soft</term><term>Palatine Tonsil</term><term>Rabies</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Rabies</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Rabies</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term><term>Animals</term><term>Base Sequence</term><term>Fluorescent Antibody Technique</term><term>Foxes</term><term>Molecular Sequence Data</term><term>Oligonucleotide Probes</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The primary multiplication site of VVTGgRAB, a recombinant vaccinia virus (VV) expressing the rabies virus G glycoprotein, was studied in comparison with that of the parental VV Copenhagen strain, after oral administration to foxes. Foxes were fed with 10(8) TCID50 of either VVTGgRAB or VV and were sacrificed 12, 24, 48 or 96 h after inoculation. Both viruses were detected by viral isolation in the tonsils during the first 48 h after inoculation at titres between 10(2) and 10(4.3) TCID50/ml. Indirect immunofluorescence confirmed the presence of the virus in tonsils of some of the foxes. The polymerase chain reaction allowed the detection of VVTGgRAB in the tonsils of both of two foxes tested after 24 h, three of three foxes after 48 h, in the buccal mucosa of one of two foxes tested after 24 h and two of three foxes after 48 h and in the soft palate of one of two foxes tested after 24 h and one of three foxes after 48 h. VV was detected in the tonsils of one fox tested after 48 h, in the buccal mucosa of another fox tested after 24 h, and in the first fox after 48 h by the same reaction. Foxes were inoculated with virus isolated from fox tonsils 24 h after oral administration (with or without cell culture amplification) to perform back passages. No virus could be isolated in either case after this passage. The innocuity of VVTGgRAB was also demonstrated when foxes were inoculated with passaged virus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2406370</PMID><DateCreated><Year>1990</Year><Month>03</Month><Day>23</Day></DateCreated><DateCompleted><Year>1990</Year><Month>03</Month><Day>23</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>71 ( Pt 1)</Volume><PubDate><Year>1990</Year><Month>Jan</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. 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The polymerase chain reaction allowed the detection of VVTGgRAB in the tonsils of both of two foxes tested after 24 h, three of three foxes after 48 h, in the buccal mucosa of one of two foxes tested after 24 h and two of three foxes after 48 h and in the soft palate of one of two foxes tested after 24 h and one of three foxes after 48 h. VV was detected in the tonsils of one fox tested after 48 h, in the buccal mucosa of another fox tested after 24 h, and in the first fox after 48 h by the same reaction. Foxes were inoculated with virus isolated from fox tonsils 24 h after oral administration (with or without cell culture amplification) to perform back passages. No virus could be isolated in either case after this passage. The innocuity of VVTGgRAB was also demonstrated when foxes were inoculated with passaged virus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>I</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brochier</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Languet</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Blancou</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Peharpre</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Kieny</LastName><ForeName>M P</ForeName><Initials>MP</Initials></Author><Author ValidYN="Y"><LastName>Desmettre</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Chappuis</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Pastoret</LastName><ForeName>P P</ForeName><Initials>PP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015345">Oligonucleotide Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011819">Rabies Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014614">Vaccines, Synthetic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000284" MajorTopicYN="N">Administration, Oral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005589" MajorTopicYN="Y">Foxes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009061" MajorTopicYN="N">Mouth Mucosa</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015345" MajorTopicYN="N">Oligonucleotide Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010160" MajorTopicYN="N">Palate, Soft</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014066" MajorTopicYN="N">Palatine Tonsil</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011818" MajorTopicYN="N">Rabies</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011819" MajorTopicYN="N">Rabies Vaccines</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011820" MajorTopicYN="N">Rabies virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014614" MajorTopicYN="N">Vaccines, Synthetic</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014616" MajorTopicYN="N">Vaccinia virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2406370</ArticleId><ArticleId 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