Immunological tools for the assessment of both humoral and cellular immune responses in Foxes (Vulpes vulpes) using ovalbumin and cholera toxin B as an antigenic model.
Identifieur interne : 000266 ( PubMed/Corpus ); précédent : 000265; suivant : 000267Immunological tools for the assessment of both humoral and cellular immune responses in Foxes (Vulpes vulpes) using ovalbumin and cholera toxin B as an antigenic model.
Auteurs : Magali Rolland-Turner ; Guillaume Farre ; Delphine Muller ; Nelly Rouet ; Franck BoueSource :
- Vaccine [ 0264-410X ] ; 2004.
English descriptors
- KwdEn :
- Administration, Intranasal, Animals, Antibody Formation (immunology), Antibody Specificity, Antibody-Producing Cells (immunology), Antitoxins (biosynthesis), Antitoxins (immunology), Blotting, Western, Centrifugation, Density Gradient, Cholera Toxin (immunology), Cross Reactions, Dogs, Enzyme-Linked Immunosorbent Assay, Female, Foxes (immunology), Immunity, Cellular (immunology), Immunization, Immunoglobulin G (analysis), Immunoglobulin G (biosynthesis), Injections, Intravenous, Monocytes (immunology), Ovalbumin (immunology), Spleen (cytology), Spleen (immunology), Vagina (immunology).
- MESH :
- chemical , analysis : Immunoglobulin G.
- chemical , biosynthesis : Antitoxins, Immunoglobulin G.
- cytology : Spleen.
- immunology : Antibody Formation, Antibody-Producing Cells, Antitoxins, Cholera Toxin, Foxes, Immunity, Cellular, Monocytes, Ovalbumin, Spleen, Vagina.
- Administration, Intranasal, Animals, Antibody Specificity, Blotting, Western, Centrifugation, Density Gradient, Cross Reactions, Dogs, Enzyme-Linked Immunosorbent Assay, Female, Immunization, Injections, Intravenous.
Abstract
The immune response in the fox (Vulpes vulpes), despite the success of the oral rabies vaccine is not well characterized, and specific immunological tools are needed. To investigate both the humoral and cellular immune response, we used ovalbumin (OVA) and cholera toxin B (CTB) as an antigenic model to set-up ELISA and ELISPOT antibodies secreting cells (ASC) assays in the fox model. Identification of antibodies that cross-react with fox immunoglobulin was performed by Western blot, and their use was adapted for both the ELISA and ELISPOT ASC assay. The humoral and cellular specific immune responses were assessed after intra-muscular or intra-nasal immunization. Intra-muscular immunization resulted in the development of both cellular and humoral anti-OVA and anti-CTB responses in peripheral blood mononuclear cells (PBMCs). Immunization via the intra-nasal route resulted in the development of a cellular and humoral response against CTB in PBMCs. This immune response was confirmed using splenocytes from immunized animals by ELISPOT assay at euthanasia. Females immunized via the intra-nasal route developed specific anti-CTB IgM, IgA and IgG in vaginal fluids after the initial boost (day 26) showing that mucosal immunization produces a vaginal immune response in foxes. These immunological tools developed here are now available to be adapted to other antigenic models to facilitate further immune studies in foxes.
DOI: 10.1016/j.vaccine.2004.06.050
PubMed: 15474706
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pubmed:15474706Le document en format XML
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N" first="Nelly" last="Rouet">Nelly Rouet</name></author><author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boue">Franck Boue</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15474706</idno><idno type="pmid">15474706</idno><idno type="doi">10.1016/j.vaccine.2004.06.050</idno><idno type="wicri:Area/PubMed/Corpus">000266</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000266</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunological tools for the assessment of both humoral and cellular immune responses in Foxes (Vulpes vulpes) using ovalbumin and cholera toxin B as an antigenic model.</title><author><name sortKey="Rolland Turner, Magali" sort="Rolland Turner, Magali" uniqKey="Rolland Turner M" first="Magali" last="Rolland-Turner">Magali Rolland-Turner</name><affiliation><nlm:affiliation>AFSSA Nancy, Laboratoire d'études et de recherches sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzéville, France. m.rolland@afssa.fr</nlm:affiliation></affiliation></author><author><name sortKey="Farre, Guillaume" sort="Farre, Guillaume" uniqKey="Farre G" first="Guillaume" last="Farre">Guillaume Farre</name></author><author><name sortKey="Muller, Delphine" sort="Muller, Delphine" uniqKey="Muller D" first="Delphine" last="Muller">Delphine Muller</name></author><author><name sortKey="Rouet, Nelly" sort="Rouet, Nelly" uniqKey="Rouet N" first="Nelly" last="Rouet">Nelly Rouet</name></author><author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boue">Franck Boue</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2004" 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(immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antitoxins</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Formation</term><term>Antibody-Producing Cells</term><term>Antitoxins</term><term>Cholera Toxin</term><term>Foxes</term><term>Immunity, Cellular</term><term>Monocytes</term><term>Ovalbumin</term><term>Spleen</term><term>Vagina</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Antibody Specificity</term><term>Blotting, Western</term><term>Centrifugation, Density Gradient</term><term>Cross Reactions</term><term>Dogs</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Immunization</term><term>Injections, Intravenous</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The immune response in the fox (Vulpes vulpes), despite the success of the oral rabies vaccine is not well characterized, and specific immunological tools are needed. To investigate both the humoral and cellular immune response, we used ovalbumin (OVA) and cholera toxin B (CTB) as an antigenic model to set-up ELISA and ELISPOT antibodies secreting cells (ASC) assays in the fox model. Identification of antibodies that cross-react with fox immunoglobulin was performed by Western blot, and their use was adapted for both the ELISA and ELISPOT ASC assay. The humoral and cellular specific immune responses were assessed after intra-muscular or intra-nasal immunization. Intra-muscular immunization resulted in the development of both cellular and humoral anti-OVA and anti-CTB responses in peripheral blood mononuclear cells (PBMCs). Immunization via the intra-nasal route resulted in the development of a cellular and humoral response against CTB in PBMCs. This immune response was confirmed using splenocytes from immunized animals by ELISPOT assay at euthanasia. Females immunized via the intra-nasal route developed specific anti-CTB IgM, IgA and IgG in vaginal fluids after the initial boost (day 26) showing that mucosal immunization produces a vaginal immune response in foxes. These immunological tools developed here are now available to be adapted to other antigenic models to facilitate further immune studies in foxes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15474706</PMID><DateCreated><Year>2004</Year><Month>10</Month><Day>11</Day></DateCreated><DateCompleted><Year>2004</Year><Month>12</Month><Day>27</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0264-410X</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>31-32</Issue><PubDate><Year>2004</Year><Month>Oct</Month><Day>22</Day></PubDate></JournalIssue><Title>Vaccine</Title><ISOAbbreviation>Vaccine</ISOAbbreviation></Journal><ArticleTitle>Immunological tools for the assessment of both humoral and cellular immune responses in Foxes (Vulpes vulpes) using ovalbumin and cholera toxin B as an antigenic model.</ArticleTitle><Pagination><MedlinePgn>4163-72</MedlinePgn></Pagination><Abstract><AbstractText>The immune response in the fox (Vulpes vulpes), despite the success of the oral rabies vaccine is not well characterized, and specific immunological tools are needed. To investigate both the humoral and cellular immune response, we used ovalbumin (OVA) and cholera toxin B (CTB) as an antigenic model to set-up ELISA and ELISPOT antibodies secreting cells (ASC) assays in the fox model. Identification of antibodies that cross-react with fox immunoglobulin was performed by Western blot, and their use was adapted for both the ELISA and ELISPOT ASC assay. The humoral and cellular specific immune responses were assessed after intra-muscular or intra-nasal immunization. Intra-muscular immunization resulted in the development of both cellular and humoral anti-OVA and anti-CTB responses in peripheral blood mononuclear cells (PBMCs). Immunization via the intra-nasal route resulted in the development of a cellular and humoral response against CTB in PBMCs. This immune response was confirmed using splenocytes from immunized animals by ELISPOT assay at euthanasia. Females immunized via the intra-nasal route developed specific anti-CTB IgM, IgA and IgG in vaginal fluids after the initial boost (day 26) showing that mucosal immunization produces a vaginal immune response in foxes. These immunological tools developed here are now available to be adapted to other antigenic models to facilitate further immune studies in foxes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rolland-Turner</LastName><ForeName>Magali</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>AFSSA Nancy, Laboratoire d'études et de recherches sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzéville, France. m.rolland@afssa.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Farre</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Muller</LastName><ForeName>Delphine</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Rouet</LastName><ForeName>Nelly</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Boue</LastName><ForeName>Franck</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Vaccine</MedlineTA><NlmUniqueID>8406899</NlmUniqueID><ISSNLinking>0264-410X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000992">Antitoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>9006-59-1</RegistryNumber><NameOfSubstance UI="D010047">Ovalbumin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9012-63-9</RegistryNumber><NameOfSubstance UI="D002772">Cholera Toxin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000281" MajorTopicYN="N">Administration, Intranasal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000917" MajorTopicYN="N">Antibody Formation</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="N">Antibody Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000921" MajorTopicYN="N">Antibody-Producing Cells</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000992" MajorTopicYN="N">Antitoxins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000276" 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