Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses.
Identifieur interne : 000221 ( PubMed/Corpus ); précédent : 000220; suivant : 000222Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses.
Auteurs : E. Picard-Meyer ; J. Barrat ; F. CliquetSource :
- Journal of virological methods [ 0166-0934 ] ; 2007.
English descriptors
- KwdEn :
- Animals, Base Sequence, Biotechnology (methods), Cell Line, Cloning, Molecular, Cricetinae, DNA Primers, Dogs, Evaluation Studies as Topic, Feasibility Studies, Filtration (instrumentation), Filtration (veterinary), Foxes, Lyssavirus (genetics), Molecular Sequence Data, Nucleic Acid Amplification Techniques, Paper, RNA, Viral (analysis), Rabies virus (genetics), Rabies virus (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Sensitivity and Specificity, Sequence Homology, Nucleic Acid, Specimen Handling (instrumentation), Specimen Handling (veterinary), Time Factors.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical : DNA Primers.
- genetics : Lyssavirus, Rabies virus.
- instrumentation : Filtration, Specimen Handling.
- isolation & purification : Rabies virus.
- methods : Biotechnology.
- veterinary : Filtration, Reverse Transcriptase Polymerase Chain Reaction, Specimen Handling.
- Animals, Base Sequence, Cell Line, Cloning, Molecular, Cricetinae, Dogs, Evaluation Studies as Topic, Feasibility Studies, Foxes, Molecular Sequence Data, Nucleic Acid Amplification Techniques, Paper, Sensitivity and Specificity, Sequence Homology, Nucleic Acid, Time Factors.
Abstract
This study evaluates the feasibility of the use of the FTA Gene Guard System (a commercial product consisting of filter paper impregnated with patented chemicals supplied by the Whatman company) for the shipment, storage and detection of RNA rabies viruses by a simplified hemi-nested reverse transcriptase polymerase chain reaction. HnRT-PCR of the rabies virus nucleoprotein gene with specific primers showed that viral RNA extracted from crude infected tissues remained stable after fixation on the filter paper under diverse environmental conditions for at least 35 days. The sequence analysis of the products amplified from five out of the seven known genotypes of Lyssaviruses showed the stability of viral RNA viruses after fixation on the filter paper. Furthermore, the sensitivity of the hnRT-PCR following RNA fixation on the filter paper was equivalent to that of standard hnRT-PCR. In conclusion, the stability of viral RNA and the inactivation of infectivity make the FTA technology useful for the storage, transport, collection and subsequent molecular analysis of viral rabies RNA, facilitating epidemiological investigations in the field.
DOI: 10.1016/j.jviromet.2006.11.011
PubMed: 17157394
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pubmed:17157394Le document en format XML
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Cliquet</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17157394</idno><idno type="pmid">17157394</idno><idno type="doi">10.1016/j.jviromet.2006.11.011</idno><idno type="wicri:Area/PubMed/Corpus">000221</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000221</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses.</title><author><name sortKey="Picard Meyer, E" sort="Picard Meyer, E" uniqKey="Picard Meyer E" first="E" last="Picard-Meyer">E. Picard-Meyer</name><affiliation><nlm:affiliation>National Laboratory of Research on Rabies and Wildlife Diseases, Community Reference Institute for Rabies Serology, AFSSA Nancy, BP 40009, F-54220 Malzéville, France. e.picard@nancy.afssa.fr</nlm:affiliation></affiliation></author><author><name sortKey="Barrat, J" sort="Barrat, J" uniqKey="Barrat J" first="J" last="Barrat">J. Barrat</name></author><author><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Biotechnology (methods)</term><term>Cell Line</term><term>Cloning, Molecular</term><term>Cricetinae</term><term>DNA Primers</term><term>Dogs</term><term>Evaluation Studies as Topic</term><term>Feasibility Studies</term><term>Filtration (instrumentation)</term><term>Filtration (veterinary)</term><term>Foxes</term><term>Lyssavirus (genetics)</term><term>Molecular Sequence Data</term><term>Nucleic Acid Amplification Techniques</term><term>Paper</term><term>RNA, Viral (analysis)</term><term>Rabies virus (genetics)</term><term>Rabies virus (isolation & purification)</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Sensitivity and Specificity</term><term>Sequence Homology, Nucleic Acid</term><term>Specimen Handling (instrumentation)</term><term>Specimen Handling (veterinary)</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lyssavirus</term><term>Rabies virus</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Filtration</term><term>Specimen Handling</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Rabies virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Filtration</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Specimen Handling</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Cloning, Molecular</term><term>Cricetinae</term><term>Dogs</term><term>Evaluation Studies as Topic</term><term>Feasibility Studies</term><term>Foxes</term><term>Molecular Sequence Data</term><term>Nucleic Acid Amplification Techniques</term><term>Paper</term><term>Sensitivity and Specificity</term><term>Sequence Homology, Nucleic Acid</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study evaluates the feasibility of the use of the FTA Gene Guard System (a commercial product consisting of filter paper impregnated with patented chemicals supplied by the Whatman company) for the shipment, storage and detection of RNA rabies viruses by a simplified hemi-nested reverse transcriptase polymerase chain reaction. HnRT-PCR of the rabies virus nucleoprotein gene with specific primers showed that viral RNA extracted from crude infected tissues remained stable after fixation on the filter paper under diverse environmental conditions for at least 35 days. The sequence analysis of the products amplified from five out of the seven known genotypes of Lyssaviruses showed the stability of viral RNA viruses after fixation on the filter paper. Furthermore, the sensitivity of the hnRT-PCR following RNA fixation on the filter paper was equivalent to that of standard hnRT-PCR. In conclusion, the stability of viral RNA and the inactivation of infectivity make the FTA technology useful for the storage, transport, collection and subsequent molecular analysis of viral rabies RNA, facilitating epidemiological investigations in the field.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17157394</PMID><DateCreated><Year>2007</Year><Month>02</Month><Day>05</Day></DateCreated><DateCompleted><Year>2007</Year><Month>04</Month><Day>11</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0166-0934</ISSN><JournalIssue CitedMedium="Print"><Volume>140</Volume><Issue>1-2</Issue><PubDate><Year>2007</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of virological methods</Title><ISOAbbreviation>J. Virol. Methods</ISOAbbreviation></Journal><ArticleTitle>Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses.</ArticleTitle><Pagination><MedlinePgn>174-82</MedlinePgn></Pagination><Abstract><AbstractText>This study evaluates the feasibility of the use of the FTA Gene Guard System (a commercial product consisting of filter paper impregnated with patented chemicals supplied by the Whatman company) for the shipment, storage and detection of RNA rabies viruses by a simplified hemi-nested reverse transcriptase polymerase chain reaction. HnRT-PCR of the rabies virus nucleoprotein gene with specific primers showed that viral RNA extracted from crude infected tissues remained stable after fixation on the filter paper under diverse environmental conditions for at least 35 days. The sequence analysis of the products amplified from five out of the seven known genotypes of Lyssaviruses showed the stability of viral RNA viruses after fixation on the filter paper. Furthermore, the sensitivity of the hnRT-PCR following RNA fixation on the filter paper was equivalent to that of standard hnRT-PCR. In conclusion, the stability of viral RNA and the inactivation of infectivity make the FTA technology useful for the storage, transport, collection and subsequent molecular analysis of viral rabies RNA, facilitating epidemiological investigations in the field.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Picard-Meyer</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>National Laboratory of Research on Rabies and Wildlife Diseases, Community Reference Institute for Rabies Serology, AFSSA Nancy, BP 40009, F-54220 Malzéville, France. e.picard@nancy.afssa.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barrat</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Cliquet</LastName><ForeName>F</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>12</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Virol Methods</MedlineTA><NlmUniqueID>8005839</NlmUniqueID><ISSNLinking>0166-0934</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" 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UI="D005374" MajorTopicYN="N">Filtration</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005589" MajorTopicYN="N">Foxes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018114" MajorTopicYN="N">Lyssavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="N">Nucleic Acid Amplification Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010209" MajorTopicYN="Y">Paper</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011820" MajorTopicYN="N">Rabies virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013048" MajorTopicYN="N">Specimen Handling</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000662" 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