Genetic characterisation of attenuated SAD rabies virus strains used for oral vaccination of wildlife.
Identifieur interne : 000195 ( PubMed/Corpus ); précédent : 000194; suivant : 000196Genetic characterisation of attenuated SAD rabies virus strains used for oral vaccination of wildlife.
Auteurs : Lutz Geue ; Susann Schares ; Christina Schnick ; Jeannette Kliemt ; Aline Beckert ; Conrad Freuling ; Franz J. Conraths ; Bernd Hoffmann ; Reto Zanoni ; Denise Marston ; Lorraine Mcelhinney ; Nicholas Johnson ; Anthony R. Fooks ; Noel Tordo ; Thomas MüllerSource :
- Vaccine [ 0264-410X ] ; 2008.
English descriptors
- KwdEn :
- Animals, Base Sequence, Europe, Foxes, Genome, Viral, INDEL Mutation, Molecular Sequence Data, Phylogeny, RNA, Viral (genetics), Rabies (prevention & control), Rabies (veterinary), Rabies Vaccines, Rabies virus (genetics), Recombination, Genetic, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Vaccines, Attenuated.
- MESH :
- chemical , genetics : RNA, Viral.
- geographic : Europe, Rabies Vaccines, Vaccines, Attenuated.
- genetics : Rabies virus.
- prevention & control : Rabies.
- veterinary : Rabies.
- Animals, Base Sequence, Foxes, Genome, Viral, INDEL Mutation, Molecular Sequence Data, Phylogeny, Recombination, Genetic, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology.
Abstract
The elimination of rabies from the red fox (Vulpes vulpes) in Western Europe has been achieved by the oral rabies vaccination (ORV) of wildlife with a range of attenuated rabies virus strains. With the exception of the vaccinia rabies glycoprotein recombinant vaccine (VRG), all strains were originally derived from a common ancestor; the Street Alabama Dufferin (SAD) field strain. However, after more than 30 years of ORV it is still not possible to distinguish these vaccine strains and there is little information on the genetic basis for their attenuation. We therefore sequenced and compared the full-length genome of five commercially available SAD vaccine viruses (SAD B19, SAD P5/88, SAG2, SAD VA1 and SAD Bern) and four other SAD strains (the original SAD Bern, SAD VA1, ERA and SAD 1-3670 Wistar). Nucleotide sequencing allowed identifying each vaccine strain unambiguously. Phylogenetic analysis revealed that the majority of the currently used commercial attenuated rabies virus vaccines appear to be derived from SAD B19 rather than from SAD Bern. One commercially available vaccine virus did not contain the SAD strain mentioned in the product information of the producer. Two SAD vaccine strains appeared to consist of mixed genomic sequences. Furthermore, in-del events targeting A-rich sequences (in positive strand) within the 3' non-coding regions of M and G genes were observed in SAD-derivates developed in Europe. Our data also supports the idea of a possible recombination that had occurred during the derivation of the European branch of SAD viruses. If confirmed, this recombination event would be the first one reported among RABV vaccine strains.
DOI: 10.1016/j.vaccine.2008.04.007
PubMed: 18485548
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Conraths</name></author><author><name sortKey="Hoffmann, Bernd" sort="Hoffmann, Bernd" uniqKey="Hoffmann B" first="Bernd" last="Hoffmann">Bernd Hoffmann</name></author><author><name sortKey="Zanoni, Reto" sort="Zanoni, Reto" uniqKey="Zanoni R" first="Reto" last="Zanoni">Reto Zanoni</name></author><author><name sortKey="Marston, Denise" sort="Marston, Denise" uniqKey="Marston D" first="Denise" last="Marston">Denise Marston</name></author><author><name sortKey="Mcelhinney, Lorraine" sort="Mcelhinney, Lorraine" uniqKey="Mcelhinney L" first="Lorraine" last="Mcelhinney">Lorraine Mcelhinney</name></author><author><name sortKey="Johnson, Nicholas" sort="Johnson, Nicholas" uniqKey="Johnson N" first="Nicholas" last="Johnson">Nicholas Johnson</name></author><author><name sortKey="Fooks, Anthony R" sort="Fooks, Anthony R" uniqKey="Fooks A" first="Anthony R" last="Fooks">Anthony R. Fooks</name></author><author><name sortKey="Tordo, Noel" sort="Tordo, Noel" uniqKey="Tordo N" first="Noel" last="Tordo">Noel Tordo</name></author><author><name sortKey="Muller, Thomas" sort="Muller, Thomas" uniqKey="Muller T" first="Thomas" last="Müller">Thomas Müller</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18485548</idno><idno type="pmid">18485548</idno><idno type="doi">10.1016/j.vaccine.2008.04.007</idno><idno type="wicri:Area/PubMed/Corpus">000195</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000195</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic characterisation of attenuated SAD rabies virus strains used for oral vaccination of wildlife.</title><author><name sortKey="Geue, Lutz" sort="Geue, Lutz" uniqKey="Geue L" first="Lutz" last="Geue">Lutz Geue</name><affiliation><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, WHO Collaborating Centre for Rabies Surveillance and Research, Wusterhausen, Germany. lutz.geue@fli.bund.de</nlm:affiliation></affiliation></author><author><name sortKey="Schares, Susann" sort="Schares, Susann" uniqKey="Schares S" first="Susann" last="Schares">Susann Schares</name></author><author><name sortKey="Schnick, Christina" sort="Schnick, Christina" uniqKey="Schnick C" first="Christina" last="Schnick">Christina Schnick</name></author><author><name sortKey="Kliemt, Jeannette" sort="Kliemt, Jeannette" uniqKey="Kliemt J" first="Jeannette" last="Kliemt">Jeannette Kliemt</name></author><author><name sortKey="Beckert, Aline" sort="Beckert, Aline" uniqKey="Beckert A" first="Aline" last="Beckert">Aline Beckert</name></author><author><name sortKey="Freuling, Conrad" sort="Freuling, Conrad" uniqKey="Freuling C" first="Conrad" last="Freuling">Conrad Freuling</name></author><author><name sortKey="Conraths, Franz J" sort="Conraths, Franz J" uniqKey="Conraths F" first="Franz J" last="Conraths">Franz J. Conraths</name></author><author><name sortKey="Hoffmann, Bernd" sort="Hoffmann, Bernd" uniqKey="Hoffmann B" first="Bernd" last="Hoffmann">Bernd Hoffmann</name></author><author><name sortKey="Zanoni, Reto" sort="Zanoni, Reto" uniqKey="Zanoni R" first="Reto" last="Zanoni">Reto Zanoni</name></author><author><name sortKey="Marston, Denise" sort="Marston, Denise" uniqKey="Marston D" first="Denise" last="Marston">Denise Marston</name></author><author><name sortKey="Mcelhinney, Lorraine" sort="Mcelhinney, Lorraine" uniqKey="Mcelhinney L" first="Lorraine" last="Mcelhinney">Lorraine Mcelhinney</name></author><author><name sortKey="Johnson, Nicholas" sort="Johnson, Nicholas" uniqKey="Johnson N" first="Nicholas" last="Johnson">Nicholas Johnson</name></author><author><name sortKey="Fooks, Anthony R" sort="Fooks, Anthony R" uniqKey="Fooks A" first="Anthony R" last="Fooks">Anthony R. Fooks</name></author><author><name sortKey="Tordo, Noel" sort="Tordo, Noel" uniqKey="Tordo N" first="Noel" last="Tordo">Noel Tordo</name></author><author><name sortKey="Muller, Thomas" sort="Muller, Thomas" uniqKey="Muller T" first="Thomas" last="Müller">Thomas Müller</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Europe</term><term>Foxes</term><term>Genome, Viral</term><term>INDEL Mutation</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>RNA, Viral (genetics)</term><term>Rabies (prevention & control)</term><term>Rabies (veterinary)</term><term>Rabies Vaccines</term><term>Rabies virus (genetics)</term><term>Recombination, Genetic</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Europe</term><term>Rabies Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rabies virus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Rabies</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Rabies</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Foxes</term><term>Genome, Viral</term><term>INDEL Mutation</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Recombination, Genetic</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The elimination of rabies from the red fox (Vulpes vulpes) in Western Europe has been achieved by the oral rabies vaccination (ORV) of wildlife with a range of attenuated rabies virus strains. With the exception of the vaccinia rabies glycoprotein recombinant vaccine (VRG), all strains were originally derived from a common ancestor; the Street Alabama Dufferin (SAD) field strain. However, after more than 30 years of ORV it is still not possible to distinguish these vaccine strains and there is little information on the genetic basis for their attenuation. We therefore sequenced and compared the full-length genome of five commercially available SAD vaccine viruses (SAD B19, SAD P5/88, SAG2, SAD VA1 and SAD Bern) and four other SAD strains (the original SAD Bern, SAD VA1, ERA and SAD 1-3670 Wistar). Nucleotide sequencing allowed identifying each vaccine strain unambiguously. Phylogenetic analysis revealed that the majority of the currently used commercial attenuated rabies virus vaccines appear to be derived from SAD B19 rather than from SAD Bern. One commercially available vaccine virus did not contain the SAD strain mentioned in the product information of the producer. Two SAD vaccine strains appeared to consist of mixed genomic sequences. Furthermore, in-del events targeting A-rich sequences (in positive strand) within the 3' non-coding regions of M and G genes were observed in SAD-derivates developed in Europe. Our data also supports the idea of a possible recombination that had occurred during the derivation of the European branch of SAD viruses. If confirmed, this recombination event would be the first one reported among RABV vaccine strains.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18485548</PMID><DateCreated><Year>2008</Year><Month>06</Month><Day>13</Day></DateCreated><DateCompleted><Year>2008</Year><Month>09</Month><Day>09</Day></DateCompleted><DateRevised><Year>2008</Year><Month>06</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0264-410X</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>26</Issue><PubDate><Year>2008</Year><Month>Jun</Month><Day>19</Day></PubDate></JournalIssue><Title>Vaccine</Title><ISOAbbreviation>Vaccine</ISOAbbreviation></Journal><ArticleTitle>Genetic characterisation of attenuated SAD rabies virus strains used for oral vaccination of wildlife.</ArticleTitle><Pagination><MedlinePgn>3227-35</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.vaccine.2008.04.007</ELocationID><Abstract><AbstractText>The elimination of rabies from the red fox (Vulpes vulpes) in Western Europe has been achieved by the oral rabies vaccination (ORV) of wildlife with a range of attenuated rabies virus strains. With the exception of the vaccinia rabies glycoprotein recombinant vaccine (VRG), all strains were originally derived from a common ancestor; the Street Alabama Dufferin (SAD) field strain. However, after more than 30 years of ORV it is still not possible to distinguish these vaccine strains and there is little information on the genetic basis for their attenuation. We therefore sequenced and compared the full-length genome of five commercially available SAD vaccine viruses (SAD B19, SAD P5/88, SAG2, SAD VA1 and SAD Bern) and four other SAD strains (the original SAD Bern, SAD VA1, ERA and SAD 1-3670 Wistar). Nucleotide sequencing allowed identifying each vaccine strain unambiguously. Phylogenetic analysis revealed that the majority of the currently used commercial attenuated rabies virus vaccines appear to be derived from SAD B19 rather than from SAD Bern. One commercially available vaccine virus did not contain the SAD strain mentioned in the product information of the producer. Two SAD vaccine strains appeared to consist of mixed genomic sequences. Furthermore, in-del events targeting A-rich sequences (in positive strand) within the 3' non-coding regions of M and G genes were observed in SAD-derivates developed in Europe. Our data also supports the idea of a possible recombination that had occurred during the derivation of the European branch of SAD viruses. If confirmed, this recombination event would be the first one reported among RABV vaccine strains.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Geue</LastName><ForeName>Lutz</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, WHO Collaborating Centre for Rabies Surveillance and Research, Wusterhausen, Germany. lutz.geue@fli.bund.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schares</LastName><ForeName>Susann</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Schnick</LastName><ForeName>Christina</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Kliemt</LastName><ForeName>Jeannette</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Beckert</LastName><ForeName>Aline</ForeName><Initials>A</Initials></Author><Author 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UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>04</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Vaccine</MedlineTA><NlmUniqueID>8406899</NlmUniqueID><ISSNLinking>0264-410X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011819">Rabies Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014613">Vaccines, Attenuated</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005060" MajorTopicYN="N" Type="Geographic">Europe</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005589" MajorTopicYN="N">Foxes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054643" MajorTopicYN="N">INDEL Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011818" MajorTopicYN="N">Rabies</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & 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Attenuated</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2007</Year><Month>12</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>04</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>5</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>9</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>5</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18485548</ArticleId><ArticleId IdType="pii">S0264-410X(08)00440-4</ArticleId><ArticleId 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