Segmental sedimentation and counting technique (SSCT): an adaptable method for qualitative diagnosis of Echinococcus multilocularis in fox intestines.
Identifieur interne : 000148 ( PubMed/Corpus ); précédent : 000147; suivant : 000149Segmental sedimentation and counting technique (SSCT): an adaptable method for qualitative diagnosis of Echinococcus multilocularis in fox intestines.
Auteurs : G. Umhang ; N. Woronoff-Rhen ; B. Combes ; F. BouéSource :
- Experimental parasitology [ 1090-2449 ] ; 2011.
English descriptors
- KwdEn :
- Animals, Echinococcosis (diagnosis), Echinococcosis (parasitology), Echinococcosis (veterinary), Echinococcus multilocularis (growth & development), Echinococcus multilocularis (isolation & purification), Foxes (parasitology), Intestinal Diseases, Parasitic (diagnosis), Intestinal Diseases, Parasitic (parasitology), Intestinal Diseases, Parasitic (veterinary), Intestines (parasitology), Sensitivity and Specificity.
- MESH :
- diagnosis : Echinococcosis, Intestinal Diseases, Parasitic.
- growth & development : Echinococcus multilocularis.
- isolation & purification : Echinococcus multilocularis.
- parasitology : Echinococcosis, Foxes, Intestinal Diseases, Parasitic, Intestines.
- veterinary : Echinococcosis, Intestinal Diseases, Parasitic.
- Animals, Sensitivity and Specificity.
Abstract
A modified Segmental Sedimentation and Counting Technique (SSCT) to examine the presence of Echinococcus multilocularis helminths in segments of fox (Vulpes vulpes) intestine is described and compared to the "gold standard", SCT. Out of the 358 intestines collected, 117 were E. multilocularis positive. Using SSCT methods we compare the sensitivity of individual or pairs of segments to establish a tradeoff between saving time and the reliability of the diagnosis, especially in areas with low infection intensities. The results show that the analysis of segment S4 associated with segment S1 or S2 give 98.3% sensitivity, with specificity close to 100%. Based on our results and the time saved, we recommend using SSCT for routine examination of fox intestines for large epidemiological studies, particularly where the endemic prevalence of E. multilocularis is low or unknown.
DOI: 10.1016/j.exppara.2011.01.004
PubMed: 21256846
Links to Exploration step
pubmed:21256846Le document en format XML
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Umhang</name><affiliation><nlm:affiliation>French Agency for Food, Environmental and Occupational Health and Safety (Anses), Nancy Rabies and Wildlife Laboratory, Technopole Agricole et Vétérinaire, Malzéville, France.</nlm:affiliation></affiliation></author><author><name sortKey="Woronoff Rhen, N" sort="Woronoff Rhen, N" uniqKey="Woronoff Rhen N" first="N" last="Woronoff-Rhen">N. Woronoff-Rhen</name></author><author><name sortKey="Combes, B" sort="Combes, B" uniqKey="Combes B" first="B" last="Combes">B. Combes</name></author><author><name sortKey="Boue, F" sort="Boue, F" uniqKey="Boue F" first="F" last="Boué">F. Boué</name></author></analytic><series><title level="j">Experimental parasitology</title><idno type="eISSN">1090-2449</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Echinococcosis (diagnosis)</term><term>Echinococcosis (parasitology)</term><term>Echinococcosis (veterinary)</term><term>Echinococcus multilocularis (growth & development)</term><term>Echinococcus multilocularis (isolation & purification)</term><term>Foxes (parasitology)</term><term>Intestinal Diseases, Parasitic (diagnosis)</term><term>Intestinal Diseases, Parasitic (parasitology)</term><term>Intestinal Diseases, Parasitic (veterinary)</term><term>Intestines (parasitology)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Echinococcosis</term><term>Intestinal Diseases, Parasitic</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Echinococcus multilocularis</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Echinococcus multilocularis</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Echinococcosis</term><term>Foxes</term><term>Intestinal Diseases, Parasitic</term><term>Intestines</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Echinococcosis</term><term>Intestinal Diseases, Parasitic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A modified Segmental Sedimentation and Counting Technique (SSCT) to examine the presence of Echinococcus multilocularis helminths in segments of fox (Vulpes vulpes) intestine is described and compared to the "gold standard", SCT. Out of the 358 intestines collected, 117 were E. multilocularis positive. Using SSCT methods we compare the sensitivity of individual or pairs of segments to establish a tradeoff between saving time and the reliability of the diagnosis, especially in areas with low infection intensities. The results show that the analysis of segment S4 associated with segment S1 or S2 give 98.3% sensitivity, with specificity close to 100%. Based on our results and the time saved, we recommend using SSCT for routine examination of fox intestines for large epidemiological studies, particularly where the endemic prevalence of E. multilocularis is low or unknown.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21256846</PMID><DateCreated><Year>2011</Year><Month>03</Month><Day>28</Day></DateCreated><DateCompleted><Year>2011</Year><Month>05</Month><Day>17</Day></DateCompleted><DateRevised><Year>2011</Year><Month>03</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2449</ISSN><JournalIssue CitedMedium="Internet"><Volume>128</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>Experimental parasitology</Title><ISOAbbreviation>Exp. Parasitol.</ISOAbbreviation></Journal><ArticleTitle>Segmental sedimentation and counting technique (SSCT): an adaptable method for qualitative diagnosis of Echinococcus multilocularis in fox intestines.</ArticleTitle><Pagination><MedlinePgn>57-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.exppara.2011.01.004</ELocationID><Abstract><AbstractText>A modified Segmental Sedimentation and Counting Technique (SSCT) to examine the presence of Echinococcus multilocularis helminths in segments of fox (Vulpes vulpes) intestine is described and compared to the "gold standard", SCT. Out of the 358 intestines collected, 117 were E. multilocularis positive. Using SSCT methods we compare the sensitivity of individual or pairs of segments to establish a tradeoff between saving time and the reliability of the diagnosis, especially in areas with low infection intensities. The results show that the analysis of segment S4 associated with segment S1 or S2 give 98.3% sensitivity, with specificity close to 100%. Based on our results and the time saved, we recommend using SSCT for routine examination of fox intestines for large epidemiological studies, particularly where the endemic prevalence of E. multilocularis is low or unknown.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Umhang</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>French Agency for Food, Environmental and Occupational Health and Safety (Anses), Nancy Rabies and Wildlife Laboratory, Technopole Agricole et Vétérinaire, Malzéville, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Woronoff-Rhen</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Combes</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Boué</LastName><ForeName>F</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>01</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Exp Parasitol</MedlineTA><NlmUniqueID>0370713</NlmUniqueID><ISSNLinking>0014-4894</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004443" MajorTopicYN="N">Echinococcosis</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D048210" MajorTopicYN="N">Echinococcus multilocularis</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005589" MajorTopicYN="N">Foxes</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007411" MajorTopicYN="N">Intestinal Diseases, Parasitic</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007422" MajorTopicYN="N">Intestines</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>01</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21256846</ArticleId><ArticleId IdType="pii">S0014-4894(11)00010-5</ArticleId><ArticleId IdType="doi">10.1016/j.exppara.2011.01.004</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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