RenardV1, PascalFrancis, Corpus, bibRecord, 000053

Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase

Identifieur interne : 000053 ( PascalFrancis/Corpus ); précédent : 000052; suivant : 000054

Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase

Auteurs : Yann Verdier ; Guillaume Farre ; Nelly Rouet ; Zoltan Kele ; Tamas Janaky ; Franck Boue

Source :

Journal of andrology [ 0196-3635 ] ; 2005.

RBID : Pascal:05-0364918

Descripteurs français

Pascal (Inist)
- Testicule, Spermatozoïde, Polypeptide, Cytochrome-c oxidase, Spécificité tissu, Contraception, Méthode immunologique, Appareil génital mâle, Reproduction, EC 1.9.3.1.

English descriptors

KwdEn :
- Contraception, Cytochrome-c oxidase, EC 1.9.3.1, Immunological method, Male genital system, Polypeptide, Reproduction, Spermatozoa, Testicle, Tissue specificity.

Abstract

Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a fox sperm protein of 14.7 kd. fSP8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. No glycosylation was detected, on fSP8, as shown both by deglycosylation assay and lectin labeling. To determine the fSP8 sequence, the NH₂-terminal sequence was first obtained, and a piece of cDNA was amplified from testicular RNA by Rapid Amplification of cDNA extremities polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 879 base pairs was obtained, which includes a major open reading frame coding for 128 amino acids. Mass spectrometric analyses have confirmed the position of the open reading frame. Analysis of the predicted amino acids sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated a homology with the Zinc binding site of the subunit Vb of the cytochrome c oxidase. On the C-terminal extremity, fSP8 presents a high homology to the Vb polypeptide of the cytochrome c oxidase from bovine, mouse, and human; however the 34 amino acids on the NH₂-extremity were specific to fSP8. Moreover, it was demonstrated that this sequence was testis-specific. This could contribute to the antigenicity of this protein. fSP8 is one of the first fox sperm antigens to be cloned and sequenced.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0196-3635`
A02	`01`			`@0 JOAND3`
A03		`1`		`@0 J. androl.`
A05				`@2 26`
A06				`@2 3`
A08	`01`	`1`	`ENG`	`@1 Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase`
A11	`01`	`1`		`@1 VERDIER (Yann)`
A11	`02`	`1`		`@1 FARRE (Guillaume)`
A11	`03`	`1`		`@1 ROUET (Nelly)`
A11	`04`	`1`		`@1 KELE (Zoltan)`
A11	`05`	`1`		`@1 JANAKY (Tamas)`
A11	`06`	`1`		`@1 BOUE (Franck)`
A14	`01`			`@1 AFSSA Nancy, Unité de Recherches sur la Rage et les Maladies Emergentes @2 Malzéville @3 FRA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 6 aut.`
A14	`02`			`@1 Department of Medical Chemistry, University of Szeged @2 Szeged @3 HUN @Z 1 aut. @Z 4 aut. @Z 5 aut.`
A20				`@1 319-327`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 18896 @5 354000129857280020`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 32 ref.`
A47	`01`	`1`		`@0 05-0364918`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of andrology`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a fox sperm protein of 14.7 kd. fSP8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. No glycosylation was detected, on fSP8, as shown both by deglycosylation assay and lectin labeling. To determine the fSP8 sequence, the NH₂-terminal sequence was first obtained, and a piece of cDNA was amplified from testicular RNA by Rapid Amplification of cDNA extremities polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 879 base pairs was obtained, which includes a major open reading frame coding for 128 amino acids. Mass spectrometric analyses have confirmed the position of the open reading frame. Analysis of the predicted amino acids sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated a homology with the Zinc binding site of the subunit Vb of the cytochrome c oxidase. On the C-terminal extremity, fSP8 presents a high homology to the Vb polypeptide of the cytochrome c oxidase from bovine, mouse, and human; however the 34 amino acids on the NH₂-extremity were specific to fSP8. Moreover, it was demonstrated that this sequence was testis-specific. This could contribute to the antigenicity of this protein. fSP8 is one of the first fox sperm antigens to be cloned and sequenced.
C02	`01`	`X`		`@0 002B20B`
C03	`01`	`X`	`FRE`	`@0 Testicule @5 01`
C03	`01`	`X`	`ENG`	`@0 Testicle @5 01`
C03	`01`	`X`	`SPA`	`@0 Testículo @5 01`
C03	`02`	`X`	`FRE`	`@0 Spermatozoïde @5 02`
C03	`02`	`X`	`ENG`	`@0 Spermatozoa @5 02`
C03	`02`	`X`	`SPA`	`@0 Espermatozoide @5 02`
C03	`03`	`X`	`FRE`	`@0 Polypeptide @5 03`
C03	`03`	`X`	`ENG`	`@0 Polypeptide @5 03`
C03	`03`	`X`	`SPA`	`@0 Polipéptido @5 03`
C03	`04`	`X`	`FRE`	`@0 Cytochrome-c oxidase @2 FE @5 04`
C03	`04`	`X`	`ENG`	`@0 Cytochrome-c oxidase @2 FE @5 04`
C03	`04`	`X`	`SPA`	`@0 Cytochrome-c oxidase @2 FE @5 04`
C03	`05`	`X`	`FRE`	`@0 Spécificité tissu @5 07`
C03	`05`	`X`	`ENG`	`@0 Tissue specificity @5 07`
C03	`05`	`X`	`SPA`	`@0 Especificidad tejido @5 07`
C03	`06`	`X`	`FRE`	`@0 Contraception @5 08`
C03	`06`	`X`	`ENG`	`@0 Contraception @5 08`
C03	`06`	`X`	`SPA`	`@0 Anticoncepción @5 08`
C03	`07`	`X`	`FRE`	`@0 Méthode immunologique @5 13`
C03	`07`	`X`	`ENG`	`@0 Immunological method @5 13`
C03	`07`	`X`	`SPA`	`@0 Método inmunológico @5 13`
C03	`08`	`X`	`FRE`	`@0 Appareil génital mâle @5 14`
C03	`08`	`X`	`ENG`	`@0 Male genital system @5 14`
C03	`08`	`X`	`SPA`	`@0 Aparato genital macho @5 14`
C03	`09`	`X`	`FRE`	`@0 Reproduction @5 15`
C03	`09`	`X`	`ENG`	`@0 Reproduction @5 15`
C03	`09`	`X`	`SPA`	`@0 Reproducción @5 15`
C03	`10`	`X`	`FRE`	`@0 EC 1.9.3.1 @4 CD @5 96`
C03	`10`	`X`	`ENG`	`@0 EC 1.9.3.1 @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Oxidoreductases @2 FE`
C07	`01`	`X`	`ENG`	`@0 Oxidoreductases @2 FE`
C07	`01`	`X`	`SPA`	`@0 Oxidoreductases @2 FE`
C07	`02`	`X`	`FRE`	`@0 Enzyme @2 FE`
C07	`02`	`X`	`ENG`	`@0 Enzyme @2 FE`
C07	`02`	`X`	`SPA`	`@0 Enzima @2 FE`
C07	`03`	`X`	`FRE`	`@0 Cellule germinale @5 20`
C07	`03`	`X`	`ENG`	`@0 Germinal cell @5 20`
C07	`03`	`X`	`SPA`	`@0 Célula germinal @5 20`
N21				`@1 255`

Format Inist (serveur)

NO :	PASCAL 05-0364918 INIST
ET :	Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase
AU :	VERDIER (Yann); FARRE (Guillaume); ROUET (Nelly); KELE (Zoltan); JANAKY (Tamas); BOUE (Franck)
AF :	AFSSA Nancy, Unité de Recherches sur la Rage et les Maladies Emergentes/Malzéville/France (1 aut., 2 aut., 3 aut., 6 aut.); Department of Medical Chemistry, University of Szeged/Szeged/Hongrie (1 aut., 4 aut., 5 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of andrology; ISSN 0196-3635; Coden JOAND3; Etats-Unis; Da. 2005; Vol. 26; No. 3; Pp. 319-327; Bibl. 32 ref.
LA :	Anglais
EA :	Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a fox sperm protein of 14.7 kd. fSP8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. No glycosylation was detected, on fSP8, as shown both by deglycosylation assay and lectin labeling. To determine the fSP8 sequence, the NH₂-terminal sequence was first obtained, and a piece of cDNA was amplified from testicular RNA by Rapid Amplification of cDNA extremities polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 879 base pairs was obtained, which includes a major open reading frame coding for 128 amino acids. Mass spectrometric analyses have confirmed the position of the open reading frame. Analysis of the predicted amino acids sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated a homology with the Zinc binding site of the subunit Vb of the cytochrome c oxidase. On the C-terminal extremity, fSP8 presents a high homology to the Vb polypeptide of the cytochrome c oxidase from bovine, mouse, and human; however the 34 amino acids on the NH₂-extremity were specific to fSP8. Moreover, it was demonstrated that this sequence was testis-specific. This could contribute to the antigenicity of this protein. fSP8 is one of the first fox sperm antigens to be cloned and sequenced.
CC :	002B20B
FD :	Testicule; Spermatozoïde; Polypeptide; Cytochrome-c oxidase; Spécificité tissu; Contraception; Méthode immunologique; Appareil génital mâle; Reproduction; EC 1.9.3.1
FG :	Oxidoreductases; Enzyme; Cellule germinale
ED :	Testicle; Spermatozoa; Polypeptide; Cytochrome-c oxidase; Tissue specificity; Contraception; Immunological method; Male genital system; Reproduction; EC 1.9.3.1
EG :	Oxidoreductases; Enzyme; Germinal cell
SD :	Testículo; Espermatozoide; Polipéptido; Cytochrome-c oxidase; Especificidad tejido; Anticoncepción; Método inmunológico; Aparato genital macho; Reproducción
LO :	INIST-18896.354000129857280020
ID :	05-0364918

Links to Exploration step

Pascal:05-0364918

Le document en format XML

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<front><div type="abstract" xml:lang="en">Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a fox sperm protein of 14.7 kd. fSP8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. No glycosylation was detected, on fSP8, as shown both by deglycosylation assay and lectin labeling. To determine the fSP8 sequence, the NH<sub>2</sub>
-terminal sequence was first obtained, and a piece of cDNA was amplified from testicular RNA by Rapid Amplification of cDNA extremities polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 879 base pairs was obtained, which includes a major open reading frame coding for 128 amino acids. Mass spectrometric analyses have confirmed the position of the open reading frame. Analysis of the predicted amino acids sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated a homology with the Zinc binding site of the subunit Vb of the cytochrome c oxidase. On the C-terminal extremity, fSP8 presents a high homology to the Vb polypeptide of the cytochrome c oxidase from bovine, mouse, and human; however the 34 amino acids on the NH<sub>2</sub>
-extremity were specific to fSP8. Moreover, it was demonstrated that this sequence was testis-specific. This could contribute to the antigenicity of this protein. fSP8 is one of the first fox sperm antigens to be cloned and sequenced.</div>
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<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a fox sperm protein of 14.7 kd. fSP8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. No glycosylation was detected, on fSP8, as shown both by deglycosylation assay and lectin labeling. To determine the fSP8 sequence, the NH<sub>2</sub>
-terminal sequence was first obtained, and a piece of cDNA was amplified from testicular RNA by Rapid Amplification of cDNA extremities polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 879 base pairs was obtained, which includes a major open reading frame coding for 128 amino acids. Mass spectrometric analyses have confirmed the position of the open reading frame. Analysis of the predicted amino acids sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated a homology with the Zinc binding site of the subunit Vb of the cytochrome c oxidase. On the C-terminal extremity, fSP8 presents a high homology to the Vb polypeptide of the cytochrome c oxidase from bovine, mouse, and human; however the 34 amino acids on the NH<sub>2</sub>
-extremity were specific to fSP8. Moreover, it was demonstrated that this sequence was testis-specific. This could contribute to the antigenicity of this protein. fSP8 is one of the first fox sperm antigens to be cloned and sequenced.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B20B</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Testicule</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Testicle</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Testículo</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Spermatozoïde</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Spermatozoa</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Espermatozoide</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Polypeptide</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Polypeptide</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Polipéptido</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Cytochrome-c oxidase</s0>
<s2>FE</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Cytochrome-c oxidase</s0>
<s2>FE</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Cytochrome-c oxidase</s0>
<s2>FE</s2>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Spécificité tissu</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Tissue specificity</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Especificidad tejido</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Contraception</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Contraception</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Anticoncepción</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Méthode immunologique</s0>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Immunological method</s0>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Método inmunológico</s0>
<s5>13</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Appareil génital mâle</s0>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Male genital system</s0>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Aparato genital macho</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Reproduction</s0>
<s5>15</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Reproduction</s0>
<s5>15</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Reproducción</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>EC 1.9.3.1</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>EC 1.9.3.1</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Cellule germinale</s0>
<s5>20</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Germinal cell</s0>
<s5>20</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Célula germinal</s0>
<s5>20</s5>
</fC07>
<fN21><s1>255</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 05-0364918 INIST</NO>
<ET>Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase</ET>
<AU>VERDIER (Yann); FARRE (Guillaume); ROUET (Nelly); KELE (Zoltan); JANAKY (Tamas); BOUE (Franck)</AU>
<AF>AFSSA Nancy, Unité de Recherches sur la Rage et les Maladies Emergentes/Malzéville/France (1 aut., 2 aut., 3 aut., 6 aut.); Department of Medical Chemistry, University of Szeged/Szeged/Hongrie (1 aut., 4 aut., 5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of andrology; ISSN 0196-3635; Coden JOAND3; Etats-Unis; Da. 2005; Vol. 26; No. 3; Pp. 319-327; Bibl. 32 ref.</SO>
<LA>Anglais</LA>
<EA>Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a fox sperm protein of 14.7 kd. fSP8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. No glycosylation was detected, on fSP8, as shown both by deglycosylation assay and lectin labeling. To determine the fSP8 sequence, the NH<sub>2</sub>
-terminal sequence was first obtained, and a piece of cDNA was amplified from testicular RNA by Rapid Amplification of cDNA extremities polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 879 base pairs was obtained, which includes a major open reading frame coding for 128 amino acids. Mass spectrometric analyses have confirmed the position of the open reading frame. Analysis of the predicted amino acids sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated a homology with the Zinc binding site of the subunit Vb of the cytochrome c oxidase. On the C-terminal extremity, fSP8 presents a high homology to the Vb polypeptide of the cytochrome c oxidase from bovine, mouse, and human; however the 34 amino acids on the NH<sub>2</sub>
-extremity were specific to fSP8. Moreover, it was demonstrated that this sequence was testis-specific. This could contribute to the antigenicity of this protein. fSP8 is one of the first fox sperm antigens to be cloned and sequenced.</EA>
<CC>002B20B</CC>
<FD>Testicule; Spermatozoïde; Polypeptide; Cytochrome-c oxidase; Spécificité tissu; Contraception; Méthode immunologique; Appareil génital mâle; Reproduction; EC 1.9.3.1</FD>
<FG>Oxidoreductases; Enzyme; Cellule germinale</FG>
<ED>Testicle; Spermatozoa; Polypeptide; Cytochrome-c oxidase; Tissue specificity; Contraception; Immunological method; Male genital system; Reproduction; EC 1.9.3.1</ED>
<EG>Oxidoreductases; Enzyme; Germinal cell</EG>
<SD>Testículo; Espermatozoide; Polipéptido; Cytochrome-c oxidase; Especificidad tejido; Anticoncepción; Método inmunológico; Aparato genital macho; Reproducción</SD>
<LO>INIST-18896.354000129857280020</LO>
<ID>05-0364918</ID>
</server>
</inist>
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Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase

Cloning and sequencing of cDNA encoding for the testis- specific fox (Vulpes Vulpes) sperm polypeptide Vb of the cytochrome C oxidase

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