Segmental Sedimentation and Counting Technique (SSCT): An adaptable method for qualitative diagnosis of Echinococcus multilocularis in fox intestines
Identifieur interne : 000013 ( PascalFrancis/Checkpoint ); précédent : 000012; suivant : 000014Segmental Sedimentation and Counting Technique (SSCT): An adaptable method for qualitative diagnosis of Echinococcus multilocularis in fox intestines
Auteurs : G. Umhang [France] ; N. Woronoff-Rhen [France] ; B. Combes [France] ; F. Boue [France]Source :
- Experimental parasitology [ 0014-4894 ] ; 2011.
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Abstract
A modified Segmental Sedimentation and Counting Technique (SSCT) to examine the presence of Echinococcus multilocularis helminths in segments of fox (Vulpes vulpes) intestine is described and compared to the "gold standard", SCT. Out of the 358 intestines collected, 117 were E. multilocularis positive. Using SSCT methods we compare the sensitivity of individual or pairs of segments to establish a tradeoff between saving time and the reliability of the diagnosis, especially in areas with low infection intensities. The results show that the analysis of segment S4 associated with segment S1 or S2 give 98.3% sensitivity, with specificity close to 100%. Based on our results and the time saved, we recommend using SSCT for routine examination of fox intestines for large epidemiological studies, particularly where the endemic prevalence of E. multilocularis is low or unknown.
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Boue</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>French Agency for Food, Environmental and Occupational Health and Safety (Anses), Nancy Rabies and Wildlife Laboratory, Technopole Agricole et Vétérinaire, BP 40009</s1><s2>54220 Malzéville</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Experimental parasitology</title><title level="j" type="abbreviated">Exp. parasitol.</title><idno type="ISSN">0014-4894</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Experimental parasitology</title><title level="j" type="abbreviated">Exp. parasitol.</title><idno type="ISSN">0014-4894</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Counting</term><term>Diagnosis (taxonomy)</term><term>Echinococcus multilocularis</term><term>Enumeration(counting)</term><term>Fox</term><term>Method</term><term>Parasite</term><term>Sedimentation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Sédimentation</term><term>Comptage</term><term>Dénombrement</term><term>Méthode</term><term>Diagnose</term><term>Renard</term><term>Parasite</term><term>Echinococcus multilocularis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A modified Segmental Sedimentation and Counting Technique (SSCT) to examine the presence of Echinococcus multilocularis helminths in segments of fox (Vulpes vulpes) intestine is described and compared to the "gold standard", SCT. 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Umhang</name></region><name sortKey="Boue, F" sort="Boue, F" uniqKey="Boue F" first="F." last="Boue">F. Boue</name><name sortKey="Combes, B" sort="Combes, B" uniqKey="Combes B" first="B." last="Combes">B. Combes</name><name sortKey="Woronoff Rhen, N" sort="Woronoff Rhen, N" uniqKey="Woronoff Rhen N" first="N." last="Woronoff-Rhen">N. Woronoff-Rhen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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