Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in faecal samples of foxes.
Identifieur interne : 000648 ( Ncbi/Checkpoint ); précédent : 000647; suivant : 000649Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in faecal samples of foxes.
Auteurs : P. Monnier [France] ; F. Cliquet ; M. Aubert ; S. BretagneSource :
- Veterinary parasitology [ 0304-4017 ] ; 1996.
English descriptors
- KwdEn :
- Animals, Animals, Wild, DNA Primers, DNA, Helminth (analysis), Echinococcosis (diagnosis), Echinococcosis (veterinary), Echinococcus (isolation & purification), Feces (microbiology), Foxes (microbiology), Parasite Egg Count, Polymerase Chain Reaction (methods), Polymerase Chain Reaction (veterinary), Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- chemical , analysis : DNA, Helminth.
- chemical : DNA Primers.
- diagnosis : Echinococcosis.
- isolation & purification : Echinococcus.
- methods : Polymerase Chain Reaction.
- microbiology : Feces, Foxes.
- veterinary : Echinococcosis, Polymerase Chain Reaction.
- Animals, Animals, Wild, Parasite Egg Count, Reproducibility of Results, Sensitivity and Specificity.
Abstract
A polymerase chain reaction (PCR) method was developed in order to permit a sensitive and specific identification of Echinococcus multilocularis DNA from fox faecal specimens. In an attempt to overcome problems related to the presence of endogenous PCR inhibitors in faecal extracts, we investigated a DNA extraction technique using an anion binding resin (Gene-Fizz). This simple and rapid procedure was applied to 61 faecal samples. Compared with the traditional microscopic examination, the sensitivity of PCR using Gene-Fizz was 82.3% and the specificity was 95.5%. No PCR signal was obtained for 20 Echinococcus granulosus isolates, showing that this method allowed discrimination between E. multilocularis and E. granulosus. Large-scale epidemiological surveys of fox excrements may be possible by using Gene-Fizz treatment prior to PCR amplification reactions.
PubMed: 9017867
Affiliations:
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pubmed:9017867Le document en format XML
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Aubert</name></author><author><name sortKey="Bretagne, S" sort="Bretagne, S" uniqKey="Bretagne S" first="S" last="Bretagne">S. Bretagne</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:9017867</idno><idno type="pmid">9017867</idno><idno type="wicri:Area/PubMed/Corpus">000398</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000398</idno><idno type="wicri:Area/PubMed/Curation">000398</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000398</idno><idno type="wicri:Area/PubMed/Checkpoint">000398</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000398</idno><idno type="wicri:Area/Ncbi/Merge">000648</idno><idno type="wicri:Area/Ncbi/Curation">000648</idno><idno type="wicri:Area/Ncbi/Checkpoint">000648</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in faecal samples of foxes.</title><author><name sortKey="Monnier, P" sort="Monnier, P" uniqKey="Monnier P" first="P" last="Monnier">P. Monnier</name><affiliation wicri:level="1"><nlm:affiliation>Cneva Nancy, Laboratoire d'Etudes sur la Rage et la Pathologie des Animaux sauvages, Malzeville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Cneva Nancy, Laboratoire d'Etudes sur la Rage et la Pathologie des Animaux sauvages, Malzeville</wicri:regionArea><wicri:noRegion>Malzeville</wicri:noRegion><wicri:noRegion>Malzeville</wicri:noRegion></affiliation></author><author><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name></author><author><name sortKey="Aubert, M" sort="Aubert, M" uniqKey="Aubert M" first="M" last="Aubert">M. Aubert</name></author><author><name sortKey="Bretagne, S" sort="Bretagne, S" uniqKey="Bretagne S" first="S" last="Bretagne">S. Bretagne</name></author></analytic><series><title level="j">Veterinary parasitology</title><idno type="ISSN">0304-4017</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Animals, Wild</term><term>DNA Primers</term><term>DNA, Helminth (analysis)</term><term>Echinococcosis (diagnosis)</term><term>Echinococcosis (veterinary)</term><term>Echinococcus (isolation & purification)</term><term>Feces (microbiology)</term><term>Foxes (microbiology)</term><term>Parasite Egg Count</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase Chain Reaction (veterinary)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Echinococcosis</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Echinococcus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Feces</term><term>Foxes</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Echinococcosis</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Animals, Wild</term><term>Parasite Egg Count</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A polymerase chain reaction (PCR) method was developed in order to permit a sensitive and specific identification of Echinococcus multilocularis DNA from fox faecal specimens. In an attempt to overcome problems related to the presence of endogenous PCR inhibitors in faecal extracts, we investigated a DNA extraction technique using an anion binding resin (Gene-Fizz). This simple and rapid procedure was applied to 61 faecal samples. Compared with the traditional microscopic examination, the sensitivity of PCR using Gene-Fizz was 82.3% and the specificity was 95.5%. No PCR signal was obtained for 20 Echinococcus granulosus isolates, showing that this method allowed discrimination between E. multilocularis and E. granulosus. Large-scale epidemiological surveys of fox excrements may be possible by using Gene-Fizz treatment prior to PCR amplification reactions.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Aubert, M" sort="Aubert, M" uniqKey="Aubert M" first="M" last="Aubert">M. Aubert</name><name sortKey="Bretagne, S" sort="Bretagne, S" uniqKey="Bretagne S" first="S" last="Bretagne">S. Bretagne</name><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name></noCountry><country name="France"><noRegion><name sortKey="Monnier, P" sort="Monnier, P" uniqKey="Monnier P" first="P" last="Monnier">P. Monnier</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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