Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19.
Identifieur interne : 001484 ( Main/Exploration ); précédent : 001483; suivant : 001485Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19.
Auteurs : K K Conzelmann [Allemagne] ; J H Cox ; L G Schneider ; H J ThielSource :
- Virology [ 0042-6822 ] ; 1990.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antigens, Surface (genetics), Base Sequence, Capsid (genetics), Cell Line, Cloning, Molecular, DNA, Viral (genetics), Glycoproteins (genetics), Molecular Sequence Data, Nucleoproteins (genetics), Oligonucleotide Probes, RNA, Viral (genetics), Rabies Vaccines (genetics), Rabies virus (genetics), Serial Passage, Vaccines, Attenuated (genetics), Viral Core Proteins (genetics), Viral Matrix Proteins (genetics), Viral Nonstructural Proteins, Viral Proteins (genetics).
- MESH :
- chemical , genetics : Antigens, Surface, DNA, Viral, Glycoproteins, Nucleoproteins, RNA, Viral, Rabies Vaccines, Vaccines, Attenuated, Viral Core Proteins, Viral Matrix Proteins, Viral Proteins.
- genetics : Capsid, Rabies virus.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, Molecular Sequence Data, Oligonucleotide Probes, Serial Passage, Viral Nonstructural Proteins.
Abstract
Complementary DNA spanning the entire genome of the attenuated rabies virus strain SAD B19 which is used for oral immunization of foxes in Europe was cloned and sequenced. The viral genome comprises 11,928 nucleotides and encodes the five viral proteins N, NS, M, G, and L. Deduced protein sequences are highly similar to those of the pathogenic PV strain, homologies ranging from 90.6% for the M to 98.6% for the L protein. The five cistrons are separated by intergenic regions of 2, 5, 5, and 24 nucleotides, respectively. The G transcription stop/polyadenylation consensus signal in SAD B19 is destroyed by a deletion of three A residues. The strong conservation of both noncoding and coding nucleotide sequences indicates a high selective pressure on the primary structure of rabies virus genomic RNA.
PubMed: 2139267
Affiliations:
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Le document en format XML
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first="L G" last="Schneider">L G Schneider</name></author><author><name sortKey="Thiel, H J" sort="Thiel, H J" uniqKey="Thiel H" first="H J" last="Thiel">H J Thiel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2139267</idno><idno type="pmid">2139267</idno><idno type="wicri:Area/PubMed/Corpus">000480</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000480</idno><idno type="wicri:Area/PubMed/Curation">000480</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000480</idno><idno type="wicri:Area/PubMed/Checkpoint">000480</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000480</idno><idno type="wicri:Area/Ncbi/Merge">000265</idno><idno type="wicri:Area/Ncbi/Curation">000265</idno><idno type="wicri:Area/Ncbi/Checkpoint">000265</idno><idno type="wicri:doubleKey">0042-6822:1990:Conzelmann K:molecular:cloning:and</idno><idno type="wicri:Area/Main/Merge">001588</idno><idno type="wicri:Area/Main/Curation">001484</idno><idno type="wicri:Area/Main/Exploration">001484</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19.</title><author><name sortKey="Conzelmann, K K" sort="Conzelmann, K K" uniqKey="Conzelmann K" first="K K" last="Conzelmann">K K Conzelmann</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Virus Diseases of Animals, Tübingen</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Tübingen</settlement></placeName></affiliation></author><author><name sortKey="Cox, J H" sort="Cox, J H" uniqKey="Cox J" first="J H" last="Cox">J H Cox</name></author><author><name sortKey="Schneider, L G" sort="Schneider, L G" uniqKey="Schneider L" first="L G" last="Schneider">L G Schneider</name></author><author><name sortKey="Thiel, H J" sort="Thiel, H J" uniqKey="Thiel H" first="H J" last="Thiel">H J Thiel</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antigens, Surface (genetics)</term><term>Base Sequence</term><term>Capsid (genetics)</term><term>Cell Line</term><term>Cloning, Molecular</term><term>DNA, Viral (genetics)</term><term>Glycoproteins (genetics)</term><term>Molecular Sequence Data</term><term>Nucleoproteins (genetics)</term><term>Oligonucleotide Probes</term><term>RNA, Viral (genetics)</term><term>Rabies Vaccines (genetics)</term><term>Rabies virus (genetics)</term><term>Serial Passage</term><term>Vaccines, Attenuated (genetics)</term><term>Viral Core Proteins (genetics)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Nonstructural Proteins</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Surface</term><term>DNA, Viral</term><term>Glycoproteins</term><term>Nucleoproteins</term><term>RNA, Viral</term><term>Rabies Vaccines</term><term>Vaccines, Attenuated</term><term>Viral Core Proteins</term><term>Viral Matrix Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Capsid</term><term>Rabies virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Cloning, Molecular</term><term>Molecular Sequence Data</term><term>Oligonucleotide Probes</term><term>Serial Passage</term><term>Viral Nonstructural Proteins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Complementary DNA spanning the entire genome of the attenuated rabies virus strain SAD B19 which is used for oral immunization of foxes in Europe was cloned and sequenced. The viral genome comprises 11,928 nucleotides and encodes the five viral proteins N, NS, M, G, and L. Deduced protein sequences are highly similar to those of the pathogenic PV strain, homologies ranging from 90.6% for the M to 98.6% for the L protein. The five cistrons are separated by intergenic regions of 2, 5, 5, and 24 nucleotides, respectively. The G transcription stop/polyadenylation consensus signal in SAD B19 is destroyed by a deletion of three A residues. The strong conservation of both noncoding and coding nucleotide sequences indicates a high selective pressure on the primary structure of rabies virus genomic RNA.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Tübingen</li></region><settlement><li>Tübingen</li></settlement></list><tree><noCountry><name sortKey="Cox, J H" sort="Cox, J H" uniqKey="Cox J" first="J H" last="Cox">J H Cox</name><name sortKey="Schneider, L G" sort="Schneider, L G" uniqKey="Schneider L" first="L G" last="Schneider">L G Schneider</name><name sortKey="Thiel, H J" sort="Thiel, H J" uniqKey="Thiel H" first="H J" last="Thiel">H J Thiel</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Conzelmann, K K" sort="Conzelmann, K K" uniqKey="Conzelmann K" first="K K" last="Conzelmann">K K Conzelmann</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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