Evaluation of an enzyme‐linked immunosorbent assay (ELISA) for the serological diagnosis of canine sarcoptic mange
Identifieur interne : 000907 ( Istex/Curation ); précédent : 000906; suivant : 000908Evaluation of an enzyme‐linked immunosorbent assay (ELISA) for the serological diagnosis of canine sarcoptic mange
Auteurs : Set Bornstein [Suède] ; Per Thebo [Suède] ; G Ran Zakrisson [Suède]Source :
- Veterinary Dermatology [ 0959-4493 ] ; 1996-03.
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Abstract
Abstract The purpose of this study was to evaluate a serodiagnostic test (enzyme‐linked immunosorbent assay; ELISA) for sarcoptic mange in dogs and to characterize the assay antigen, based on the mite Sarcoptes scabiei var. vulpes. The ELISA, applied to sera from 359 dogs suspected of having sarcoptic mange, showed a sensitivity and specificity of 92 and 96%, respectively. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) of the antigen employed in the ELISA revealed polypeptide bands with molecular weights ranging between 14 and 164 kDa. In Western blot analyses antigens of molecular weights between 62 and 64 kDa dominated. Particularly dominant were antigens of 164 and 147 kDa. These were found to have isoelectrical points in the range of 5.7–6.9.
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DOI: 10.1111/j.1365-3164.1996.tb00222.x
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