The New Species Brucella microti Replicates in Macrophages and Causes Death in Murine Models of Infection
Identifieur interne : 001573 ( Istex/Corpus ); précédent : 001572; suivant : 001574The New Species Brucella microti Replicates in Macrophages and Causes Death in Murine Models of Infection
Auteurs : María P. Jiménez De Bagüés ; Safia Ouahrani-Bettache ; Juan F. Quintana ; Olga Mitjana ; Nabil Hanna ; Stéphanie Bessoles ; Françoise Sanchez ; Holger C. Scholz ; Virginie Lafont ; Stephan Köhler ; Alessandra OcchialiniSource :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2010-07-01.
Abstract
Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results. B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. In experimental cellular and murine infections, B. microti exhibited a high pathogenic potential, compared with other Brucella species.
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DOI: 10.1086/653084
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Jiménez De Bagüés</name><affiliation><mods:affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</mods:affiliation></affiliation></author><author><name sortKey="Ouahrani Bettache, Safia" sort="Ouahrani Bettache, Safia" uniqKey="Ouahrani Bettache S" first="Safia" last="Ouahrani-Bettache">Safia Ouahrani-Bettache</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Quintana, Juan F" sort="Quintana, Juan F" uniqKey="Quintana J" first="Juan F." last="Quintana">Juan F. Quintana</name><affiliation><mods:affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</mods:affiliation></affiliation></author><author><name sortKey="Mitjana, Olga" sort="Mitjana, Olga" uniqKey="Mitjana O" first="Olga" last="Mitjana">Olga Mitjana</name><affiliation><mods:affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</mods:affiliation></affiliation></author><author><name sortKey="Hanna, Nabil" sort="Hanna, Nabil" uniqKey="Hanna N" first="Nabil" last="Hanna">Nabil Hanna</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Bessoles, Stephanie" sort="Bessoles, Stephanie" uniqKey="Bessoles S" first="Stéphanie" last="Bessoles">Stéphanie Bessoles</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Sanchez, Francoise" sort="Sanchez, Francoise" uniqKey="Sanchez F" first="Françoise" last="Sanchez">Françoise Sanchez</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Scholz, Holger C" sort="Scholz, Holger C" uniqKey="Scholz H" first="Holger C." last="Scholz">Holger C. Scholz</name><affiliation><mods:affiliation>Department of Bacteriology, Bundeswehr Institute of Microbiology, Germany</mods:affiliation></affiliation></author><author><name sortKey="Lafont, Virginie" sort="Lafont, Virginie" uniqKey="Lafont V" first="Virginie" last="Lafont">Virginie Lafont</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Kohler, Stephan" sort="Kohler, Stephan" uniqKey="Kohler S" first="Stephan" last="Köhler">Stephan Köhler</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Occhialini, Alessandra" sort="Occhialini, Alessandra" uniqKey="Occhialini A" first="Alessandra" last="Occhialini">Alessandra Occhialini</name><affiliation><mods:affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Université Montpellier II, Montpellier, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: aocchial@univ-montp2.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University Chicago Press</publisher><date type="published" when="2010-07-01">2010-07-01</date><biblScope unit="volume">202</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="3">3</biblScope><biblScope unit="page" to="10">10</biblScope></imprint><idno type="ISSN">0022-1899</idno></series><idno type="istex">8799C525C2DF2F14574A7157E7EB70FB20F7AE8D</idno><idno type="DOI">10.1086/653084</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results. B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. In experimental cellular and murine infections, B. microti exhibited a high pathogenic potential, compared with other Brucella species.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>María P. Jiménez de Bagüés</name><affiliations><json:string>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</json:string></affiliations></json:item><json:item><name>Safia Ouahrani-Bettache</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string></affiliations></json:item><json:item><name>Juan F. Quintana</name><affiliations><json:string>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</json:string></affiliations></json:item><json:item><name>Olga Mitjana</name><affiliations><json:string>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</json:string></affiliations></json:item><json:item><name>Nabil Hanna</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string></affiliations></json:item><json:item><name>Stéphanie Bessoles</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string></affiliations></json:item><json:item><name>Françoise Sanchez</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string></affiliations></json:item><json:item><name>Holger C. Scholz</name><affiliations><json:string>Department of Bacteriology, Bundeswehr Institute of Microbiology, Germany</json:string></affiliations></json:item><json:item><name>Virginie Lafont</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string></affiliations></json:item><json:item><name>Stephan Köhler</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string></affiliations></json:item><json:item><name>Alessandra Occhialini</name><affiliations><json:string>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</json:string><json:string>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</json:string><json:string>Université Montpellier II, Montpellier, France</json:string><json:string>E-mail: aocchial@univ-montp2.fr</json:string></affiliations></json:item></author><language><json:string>unknown</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results. B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. In experimental cellular and murine infections, B. microti exhibited a high pathogenic potential, compared with other Brucella species.</abstract><qualityIndicators><score>9.398</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1256</abstractCharCount><pdfWordCount>4998</pdfWordCount><pdfCharCount>28874</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>200</abstractWordCount></qualityIndicators><title>The New Species Brucella microti Replicates in Macrophages and Causes Death in Murine Models of 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Infection</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>The University Chicago Press</publisher><availability><p>© 2010 by the Infectious Diseases Society of America</p></availability><date>2010</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">The New Species Brucella microti Replicates in Macrophages and Causes Death in Murine Models of Infection</title><author xml:id="author-1"><persName><forename type="first">María P.</forename><surname>Jiménez de Bagüés</surname></persName><affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">Safia</forename><surname>Ouahrani-Bettache</surname></persName><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Juan F.</forename><surname>Quintana</surname></persName><affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">Olga</forename><surname>Mitjana</surname></persName><affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</affiliation></author><author xml:id="author-5"><persName><forename type="first">Nabil</forename><surname>Hanna</surname></persName><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">Stéphanie</forename><surname>Bessoles</surname></persName><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author><author xml:id="author-7"><persName><forename type="first">Françoise</forename><surname>Sanchez</surname></persName><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author><author xml:id="author-8"><persName><forename type="first">Holger C.</forename><surname>Scholz</surname></persName><affiliation>Department of Bacteriology, Bundeswehr Institute of Microbiology, Germany</affiliation></author><author xml:id="author-9"><persName><forename type="first">Virginie</forename><surname>Lafont</surname></persName><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author><author xml:id="author-10"><persName><forename type="first">Stephan</forename><surname>Köhler</surname></persName><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author><author xml:id="author-11" corresp="yes"><persName><forename type="first">Alessandra</forename><surname>Occhialini</surname></persName><email>aocchial@univ-montp2.fr</email><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation></author></analytic><monogr><title level="j">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="pISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University Chicago Press</publisher><date type="published" when="2010-07-01"></date><biblScope unit="volume">202</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="3">3</biblScope><biblScope unit="page" to="10">10</biblScope></imprint></monogr><idno type="istex">8799C525C2DF2F14574A7157E7EB70FB20F7AE8D</idno><idno type="DOI">10.1086/653084</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><abstract><p>Background. The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results. B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. In experimental cellular and murine infections, B. microti exhibited a high pathogenic potential, compared with other Brucella species.</p></abstract></profileDesc><revisionDesc><change when="2010-07-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/8799C525C2DF2F14574A7157E7EB70FB20F7AE8D/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">jinfdis</journal-id><journal-id journal-id-type="publisher-id">jid</journal-id><journal-title>The Journal of Infectious Diseases</journal-title><abbrev-journal-title>The Journal of Infectious Diseases</abbrev-journal-title><issn pub-type="ppub">0022-1899</issn><issn pub-type="epub">1537-6613</issn><publisher><publisher-name>The University Chicago Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1086/653084</article-id><article-categories><subj-group subj-group-type="heading"><subject>Major Articles and Brief Reports</subject><subj-group subj-group-type="heading"><subject>Bacteria</subject><subj-group subj-group-type="heading"><subject>Major Articles</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>The New Species <italic>Brucella microti</italic> Replicates in Macrophages and Causes Death in Murine Models of Infection</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jiménez de Bagüés</surname><given-names>María P.</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Ouahrani-Bettache</surname><given-names>Safia</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Quintana</surname><given-names>Juan F.</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Mitjana</surname><given-names>Olga</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Hanna</surname><given-names>Nabil</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Bessoles</surname><given-names>Stéphanie</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Sanchez</surname><given-names>Françoise</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Scholz</surname><given-names>Holger C.</given-names></name><xref rid="aff5" ref-type="aff">5</xref></contrib><contrib contrib-type="author"><name><surname>Lafont</surname><given-names>Virginie</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref></contrib><contrib contrib-type="author"><name><surname>Köhler</surname><given-names>Stephan</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="fn1" ref-type="fn">a</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Occhialini</surname><given-names>Alessandra</given-names></name><xref rid="cor1" ref-type="corresp"></xref><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="fn1" ref-type="fn">a</xref></contrib><aff id="aff1"><label>1</label><institution>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón</institution>, <addr-line>Zaragoza, Spain</addr-line></aff><aff id="aff2"><label>2</label><institution>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS)</institution>, <addr-line>Montpellier, France</addr-line></aff><aff id="aff3"><label>3</label><institution>UMR 5236, CPBS, Université Montpellier I</institution>, <addr-line>Montpellier, France</addr-line></aff><aff id="aff4"><label>4</label><institution>Université Montpellier II</institution>, <addr-line>Montpellier, France</addr-line></aff><aff id="aff5"><label>5</label><institution>Department of Bacteriology, Bundeswehr Institute of Microbiology</institution>, <addr-line>Germany</addr-line></aff></contrib-group><author-notes><corresp id="cor1">Reprints or correspondence: Dr Alessandra Occhialini, CBPS, UMR 5236, Université Montpellier II, cc 100, Pl. E. Bataillon, 34095 Montpellier cedex 05, France (<email>aocchial@univ-montp2.fr</email>).</corresp></author-notes><pub-date pub-type="ppub"><day>1</day><month>7</month><year>2010</year></pub-date><volume>202</volume><issue>1</issue><fpage>3</fpage><lpage>10</lpage><history><date date-type="received"><day>19</day><month>8</month><year>2009</year></date><date date-type="accepted"><day>22</day><month>10</month><year>2009</year></date></history><copyright-statement>© 2010 by the Infectious Diseases Society of America</copyright-statement><copyright-year>2010</copyright-year><abstract><p><bold><italic>Background.</italic></bold> The recent isolation of <italic>Brucella microti</italic> from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new <italic>Brucella</italic> species in both in vitro and in vivo models of infection was analyzed.</p><p><bold><italic>Methods.</italic></bold> The ability of <italic>B. microti</italic> (as compared to that of the closely related species <italic>Brucella suis</italic>) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of <italic>B. microti</italic> and <italic>B. suis</italic> was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice.</p><p><bold><italic>Results.</italic></bold> <italic>B. microti</italic> showed an enhanced capacity for intramacrophagic replication compared with that of <italic>B. suis.</italic> Surprisingly, and in contrast to other species of <italic>Brucella</italic>, 10<sup>5</sup> colony-forming units of <italic>B. microti</italic> killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with <italic>B. microti</italic> peaked at day 3, compared with <italic>B. suis</italic> infection, which peaked at day 7. Sublethal doses of <italic>B. microti</italic> induced good protection against a subsequent challenge with lethal doses.</p><p><bold><italic>Conclusions.</italic></bold> In experimental cellular and murine infections, <italic>B. microti</italic> exhibited a high pathogenic potential, compared with other <italic>Brucella</italic> species.</p></abstract></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The New Species Brucella microti Replicates in Macrophages and Causes Death in Murine Models of Infection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The New Species Brucella microti Replicates in Macrophages and Causes Death in Murine Models of Infection</title></titleInfo><name type="personal"><namePart type="given">María P.</namePart><namePart type="family">Jiménez de Bagüés</namePart><affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Safia</namePart><namePart type="family">Ouahrani-Bettache</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Juan F.</namePart><namePart type="family">Quintana</namePart><affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Olga</namePart><namePart type="family">Mitjana</namePart><affiliation>Unidad de Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Gobierno de Aragón, Zaragoza, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nabil</namePart><namePart type="family">Hanna</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stéphanie</namePart><namePart type="family">Bessoles</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise</namePart><namePart type="family">Sanchez</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Holger C.</namePart><namePart type="family">Scholz</namePart><affiliation>Department of Bacteriology, Bundeswehr Institute of Microbiology, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Virginie</namePart><namePart type="family">Lafont</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephan</namePart><namePart type="family">Köhler</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Alessandra</namePart><namePart type="family">Occhialini</namePart><affiliation>Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 5236, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France</affiliation><affiliation>UMR 5236, CPBS, Université Montpellier I, Montpellier, France</affiliation><affiliation>Université Montpellier II, Montpellier, France</affiliation><affiliation>E-mail: aocchial@univ-montp2.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>The University Chicago Press</publisher><dateIssued 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The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe. In this work, the pathogenic potential of this new Brucella species in both in vitro and in vivo models of infection was analyzed. Methods. The ability of B. microti (as compared to that of the closely related species Brucella suis) to replicate in human macrophages and in human and murine macrophage-like cells was determined. The behavior of B. microti and B. suis was evaluated in vivo in murine models of infection with Balb/c, CD1, and C57BL/6 mice. Results. B. microti showed an enhanced capacity for intramacrophagic replication compared with that of B. suis. Surprisingly, and in contrast to other species of Brucella, 105 colony-forming units of B. microti killed 82% of Balb/c mice within 7 days. Infection of spleen and liver with B. microti peaked at day 3, compared with B. suis infection, which peaked at day 7. Sublethal doses of B. microti induced good protection against a subsequent challenge with lethal doses. Conclusions. 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