Nonisotopic single‐strand conformation polymorphism analysis of sequence variability in ribosomal DNA expansion segments within the genus Trichinella (Nematoda: Adenophorea)
Identifieur interne : 000A15 ( Istex/Corpus ); précédent : 000A14; suivant : 000A16Nonisotopic single‐strand conformation polymorphism analysis of sequence variability in ribosomal DNA expansion segments within the genus Trichinella (Nematoda: Adenophorea)
Auteurs : Robin B. Gasser ; Min Hu ; Youssef G. Abs El-Osta ; Dante S. Zarlenga ; Edoardo PozioSource :
- ELECTROPHORESIS [ 0173-0835 ] ; 2004-10.
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- KwdEn :
Abstract
A nonisotopic single‐strand conformation polymorphism (SSCP) approach was employed to ‘fingerprint’ sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first‐stage) larvae representing all currently recognized species/genotypes of Trichinella. In addition, phylogenetic analyses of the D3 sequence data set, employing three different tree‐building algorithms, examined the relationships among all of them. These analyses showed strong support that the encapsulated species T. spiralis and T. nelsoni formed a group to the exclusion of the other encapsulated species T. britovi and its related genotypes Trichinella T8 and T9 and T. murrelli, and T. nativa and Trichinella T6, and strong support that T. nativa and Trichinella T6 grouped together. Also, these eight encapsulated members grouped to the exclusion of the nonencapsulated species T. papuae and T. zimbabwensis and the three representatives of T. pseudospiralis investigated. The findings showed that nonencapsulated species constitute a complex group which is distinct from the encapsulated species and supported the current hypothesis that encapsulated Trichinella group external to the nonencapsulated forms, in accordance with independent biological and biochemical data sets.
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DOI: 10.1002/elps.200405985
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ISTEX:FCB97481F8E3619CF196CDBAF1C1031799335003Le document en format XML
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In addition, phylogenetic analyses of the D3 sequence data set, employing three different tree‐building algorithms, examined the relationships among all of them. These analyses showed strong support that the encapsulated species T. spiralis and T. nelsoni formed a group to the exclusion of the other encapsulated species T. britovi and its related genotypes Trichinella T8 and T9 and T. murrelli, and T. nativa and Trichinella T6, and strong support that T. nativa and Trichinella T6 grouped together. Also, these eight encapsulated members grouped to the exclusion of the nonencapsulated species T. papuae and T. zimbabwensis and the three representatives of T. pseudospiralis investigated. The findings showed that nonencapsulated species constitute a complex group which is distinct from the encapsulated species and supported the current hypothesis that encapsulated Trichinella group external to the nonencapsulated forms, in accordance with independent biological and biochemical data sets.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Robin B. Gasser</name><affiliations><json:string>Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia</json:string><json:string>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia +61‐3‐97312366===</json:string></affiliations></json:item><json:item><name>Min Hu</name><affiliations><json:string>Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia</json:string></affiliations></json:item><json:item><name>Youssef G. 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In addition, phylogenetic analyses of the D3 sequence data set, employing three different tree‐building algorithms, examined the relationships among all of them. These analyses showed strong support that the encapsulated species T. spiralis and T. nelsoni formed a group to the exclusion of the other encapsulated species T. britovi and its related genotypes Trichinella T8 and T9 and T. murrelli, and T. nativa and Trichinella T6, and strong support that T. nativa and Trichinella T6 grouped together. Also, these eight encapsulated members grouped to the exclusion of the nonencapsulated species T. papuae and T. zimbabwensis and the three representatives of T. pseudospiralis investigated. The findings showed that nonencapsulated species constitute a complex group which is distinct from the encapsulated species and supported the current hypothesis that encapsulated Trichinella group external to the nonencapsulated forms, in accordance with independent biological and biochemical data sets.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Genetic variation</term></item><item><term>Nuclear ribosomal DNA</term></item><item><term>Phylogenetic relationships</term></item><item><term>Single‐strand conformation polymorphism analysis</term></item><item><term>Trichinella</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2004-03-23">Received</change><change when="2004-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/FCB97481F8E3619CF196CDBAF1C1031799335003/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>WILEY‐VCH Verlag</publisherName><publisherLoc>Weinheim</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1522-2683</doi><issn type="print">0173-0835</issn><issn type="electronic">1522-2683</issn><idGroup><id type="product" value="ELPS"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="ELECTROPHORESIS">ELECTROPHORESIS</title><title type="tocForm">Electrophoresis</title><title type="short">ELECTROPHORESIS</title></titleGroup></publicationMeta><publicationMeta level="part" position="200"><doi origin="wiley" registered="yes">10.1002/elps.v25:20</doi><numberingGroup><numbering type="journalVolume" number="25">25</numbering><numbering type="journalIssue">20</numbering></numberingGroup><coverDate startDate="2004-10">No. 20 October 2004</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="7" status="forIssue"><doi origin="wiley" registered="yes">10.1002/elps.200405985</doi><idGroup><id type="unit" value="ELPS200405985"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title></titleGroup><copyright ownership="publisher">Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</copyright><eventGroup><event type="manuscriptReceived" date="2004-03-23"></event><event type="firstOnline" date="2004-10-18"></event><event type="publishedOnlineFinalForm" date="2004-10-18"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.18.1 mode:FullText source:HeaderRef result:HeaderRef" date="2010-09-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-12"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst">3357</numbering><numbering type="pageLast">3364</numbering></numberingGroup><correspondenceTo>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia <b>Fax:</b> +61‐3‐97312366===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ELPS.ELPS3357.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="2"></count><count type="referenceTotal" number="28"></count></countGroup><titleGroup><title type="main" xml:lang="en">Nonisotopic single‐strand conformation polymorphism analysis of sequence variability in ribosomal DNA expansion segments within the genus <b><i>Trichinella</i></b> (Nematoda: Adenophorea)</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Robin B.</givenNames><familyName>Gasser</familyName></personName><contactDetails><email>robinbg@unimelb.edu.au</email></contactDetails></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Min</givenNames><familyName>Hu</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Youssef G.</givenNames><familyName>Abs El‐Osta</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#a2"><personName><givenNames>Dante S.</givenNames><familyName>Zarlenga</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#a3"><personName><givenNames>Edoardo</givenNames><familyName>Pozio</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="AU" type="organization"><unparsedAffiliation>Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US" type="organization"><unparsedAffiliation>US Department of Agriculture, ARS, Bovine Functional Genomics Laboratory, Beltsville, MD, USA</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="IT" type="organization"><unparsedAffiliation>Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Genetic variation</keyword><keyword xml:id="kwd2">Nuclear ribosomal DNA</keyword><keyword xml:id="kwd3">Phylogenetic relationships</keyword><keyword xml:id="kwd4">Single‐strand conformation polymorphism analysis</keyword><keyword xml:id="kwd5"><i>Trichinella</i></keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>A nonisotopic single‐strand conformation polymorphism (SSCP) approach was employed to ‘fingerprint’ sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first‐stage) larvae representing all currently recognized species/genotypes of <i>Trichinella</i>. In addition, phylogenetic analyses of the D3 sequence data set, employing three different tree‐building algorithms, examined the relationships among all of them. These analyses showed strong support that the encapsulated species <i>T. spiralis</i> and <i>T. nelsoni</i> formed a group to the exclusion of the other encapsulated species <i>T. britovi</i> and its related genotypes <i>Trichinella</i> T8 and T9 and <i>T. murrelli</i>, and <i>T</i>. <i>nativa</i> and <i>Trichinella</i> T6, and strong support that <i>T</i>. <i>nativa</i> and <i>Trichinella</i> T6 grouped together. Also, these eight encapsulated members grouped to the exclusion of the nonencapsulated species <i>T. papuae</i> and <i>T. zimbabwensis</i> and the three representatives of <i>T. pseudospiralis</i> investigated. The findings showed that nonencapsulated species constitute a complex group which is distinct from the encapsulated species and supported the current hypothesis that encapsulated <i>Trichinella</i> group external to the nonencapsulated forms, in accordance with independent biological and biochemical data sets.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Nonisotopic single‐strand conformation polymorphism analysis of sequence variability in ribosomal DNA expansion segments within the genus Trichinella (Nematoda: Adenophorea)</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Nonisotopic single‐strand conformation polymorphism analysis of sequence variability in ribosomal DNA expansion segments within the genus Trichinella (Nematoda: Adenophorea)</title></titleInfo><name type="personal"><namePart type="given">Robin B.</namePart><namePart type="family">Gasser</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia</affiliation><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia +61‐3‐97312366===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Min</namePart><namePart type="family">Hu</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Youssef G.</namePart><namePart type="family">Abs El‐Osta</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dante S.</namePart><namePart type="family">Zarlenga</namePart><affiliation>US Department of Agriculture, ARS, Bovine Functional Genomics Laboratory, Beltsville, MD, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Edoardo</namePart><namePart type="family">Pozio</namePart><affiliation>Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2004-10</dateIssued><dateCaptured encoding="w3cdtf">2004-03-23</dateCaptured><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">2</extent><extent unit="references">28</extent></physicalDescription><abstract lang="en">A nonisotopic single‐strand conformation polymorphism (SSCP) approach was employed to ‘fingerprint’ sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first‐stage) larvae representing all currently recognized species/genotypes of Trichinella. In addition, phylogenetic analyses of the D3 sequence data set, employing three different tree‐building algorithms, examined the relationships among all of them. These analyses showed strong support that the encapsulated species T. spiralis and T. nelsoni formed a group to the exclusion of the other encapsulated species T. britovi and its related genotypes Trichinella T8 and T9 and T. murrelli, and T. nativa and Trichinella T6, and strong support that T. nativa and Trichinella T6 grouped together. Also, these eight encapsulated members grouped to the exclusion of the nonencapsulated species T. papuae and T. zimbabwensis and the three representatives of T. pseudospiralis investigated. The findings showed that nonencapsulated species constitute a complex group which is distinct from the encapsulated species and supported the current hypothesis that encapsulated Trichinella group external to the nonencapsulated forms, in accordance with independent biological and biochemical data sets.</abstract><subject lang="en"><genre>keywords</genre><topic>Genetic variation</topic><topic>Nuclear ribosomal DNA</topic><topic>Phylogenetic relationships</topic><topic>Single‐strand conformation polymorphism analysis</topic><topic>Trichinella</topic></subject><relatedItem type="host"><titleInfo><title>ELECTROPHORESIS</title></titleInfo><titleInfo type="abbreviated"><title>ELECTROPHORESIS</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0173-0835</identifier><identifier type="eISSN">1522-2683</identifier><identifier type="DOI">10.1002/(ISSN)1522-2683</identifier><identifier type="PublisherID">ELPS</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><detail type="issue"><caption>no.</caption><number>20</number></detail><extent unit="pages"><start>3357</start><end>3364</end><total>8</total></extent></part></relatedItem><identifier type="istex">FCB97481F8E3619CF196CDBAF1C1031799335003</identifier><identifier type="DOI">10.1002/elps.200405985</identifier><identifier type="ArticleID">ELPS200405985</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. 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