Distribution of Trichinella spiralis larvae in muscles from a naturally infected horse
Identifieur interne : 000752 ( Istex/Corpus ); précédent : 000751; suivant : 000753Distribution of Trichinella spiralis larvae in muscles from a naturally infected horse
Auteurs : E. Pozio ; G. V. Celano ; L. Sacchi ; C. Pavia ; P. Rossi ; A. Tamburrini ; S. Corona ; G. La RosaSource :
- Veterinary Parasitology [ 0304-4017 ] ; 1998.
Abstract
Epidemiological investigations conducted during 10 trichinellosis outbreaks between 1975 and 1994 showed that horse-meat was the probable source of infection. Though hundreds of thousands of horses have been examined at abattoirs in America and Europe to detect Trichinella infection by artificial digestion or trichinelloscopy, an infected horse has never been detected during routine analysis, which consists of examining 1 g of tissue muscle from the diaphragm. In November 1996, a naturally infected horse imported from Romania was detected in Southern Italy. The parasite was identified as Trichinella spiralis by random amplified polymorphic DNA analysis. Artificial digestion of tissue samples from 60 different muscles from 13 different sites of the infected horse carcass showed that M. levator Labii maxillaris, M. hyoideus transversus, and M. buccinator were the 3 most infected muscles. Muscles from the tongue, the masseter, and the diaphragm, which have normally been considered the muscles of choice for diagnosis, were the 4th, 6th and 13th most infected muscles, respectively. When comparing body sites, muscle tissues from the head showed the highest level of infection, followed by muscles from the neck. This finding may explain the negative results that have been obtained in the past during routine examination of the diaphragm of horses.
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DOI: 10.1016/S0304-4017(97)00141-6
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Pozio</name><affiliation><mods:affiliation>E-mail: pozio@pop3.iss.it</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</mods:affiliation></affiliation></author><author><name sortKey="Celano, G V" sort="Celano, G V" uniqKey="Celano G" first="G. V." last="Celano">G. V. Celano</name><affiliation><mods:affiliation>Istituto di Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Bari, P.O. Box 7–70010 Valenzano, Bari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Sacchi, L" sort="Sacchi, L" uniqKey="Sacchi L" first="L." last="Sacchi">L. Sacchi</name><affiliation><mods:affiliation>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</mods:affiliation></affiliation></author><author><name sortKey="Pavia, C" sort="Pavia, C" uniqKey="Pavia C" first="C." last="Pavia">C. Pavia</name><affiliation><mods:affiliation>Veterinary Service, Azienda Sanitaria Locale Bari 2, Barletta 70051, Italy</mods:affiliation></affiliation></author><author><name sortKey="Rossi, P" sort="Rossi, P" uniqKey="Rossi P" first="P." last="Rossi">P. Rossi</name><affiliation><mods:affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</mods:affiliation></affiliation></author><author><name sortKey="Tamburrini, A" sort="Tamburrini, A" uniqKey="Tamburrini A" first="A." last="Tamburrini">A. Tamburrini</name><affiliation><mods:affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</mods:affiliation></affiliation></author><author><name sortKey="Corona, S" sort="Corona, S" uniqKey="Corona S" first="S." last="Corona">S. Corona</name><affiliation><mods:affiliation>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</mods:affiliation></affiliation></author><author><name sortKey="La Rosa, G" sort="La Rosa, G" uniqKey="La Rosa G" first="G." last="La Rosa">G. La Rosa</name><affiliation><mods:affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Parasitology</title><title level="j" type="abbrev">VETPAR</title><idno type="ISSN">0304-4017</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">74</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="19">19</biblScope><biblScope unit="page" to="27">27</biblScope></imprint><idno type="ISSN">0304-4017</idno></series><idno type="istex">E467CB5AC89DA0943ECA0C191902FA7A1389F032</idno><idno type="DOI">10.1016/S0304-4017(97)00141-6</idno><idno type="PII">S0304-4017(97)00141-6</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-4017</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Epidemiological investigations conducted during 10 trichinellosis outbreaks between 1975 and 1994 showed that horse-meat was the probable source of infection. Though hundreds of thousands of horses have been examined at abattoirs in America and Europe to detect Trichinella infection by artificial digestion or trichinelloscopy, an infected horse has never been detected during routine analysis, which consists of examining 1 g of tissue muscle from the diaphragm. In November 1996, a naturally infected horse imported from Romania was detected in Southern Italy. The parasite was identified as Trichinella spiralis by random amplified polymorphic DNA analysis. Artificial digestion of tissue samples from 60 different muscles from 13 different sites of the infected horse carcass showed that M. levator Labii maxillaris, M. hyoideus transversus, and M. buccinator were the 3 most infected muscles. Muscles from the tongue, the masseter, and the diaphragm, which have normally been considered the muscles of choice for diagnosis, were the 4th, 6th and 13th most infected muscles, respectively. When comparing body sites, muscle tissues from the head showed the highest level of infection, followed by muscles from the neck. This finding may explain the negative results that have been obtained in the past during routine examination of the diaphragm of horses.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>E. Pozio</name><affiliations><json:string>E-mail: pozio@pop3.iss.it</json:string><json:string>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</json:string></affiliations></json:item><json:item><name>G.V. Celano</name><affiliations><json:string>Istituto di Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Bari, P.O. Box 7–70010 Valenzano, Bari, Italy</json:string></affiliations></json:item><json:item><name>L. Sacchi</name><affiliations><json:string>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</json:string></affiliations></json:item><json:item><name>C. Pavia</name><affiliations><json:string>Veterinary Service, Azienda Sanitaria Locale Bari 2, Barletta 70051, Italy</json:string></affiliations></json:item><json:item><name>P. Rossi</name><affiliations><json:string>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</json:string></affiliations></json:item><json:item><name>A. Tamburrini</name><affiliations><json:string>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</json:string></affiliations></json:item><json:item><name>S. Corona</name><affiliations><json:string>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</json:string></affiliations></json:item><json:item><name>G. La Rosa</name><affiliations><json:string>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Horse</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Trichinella spiralis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Diagnosis nematoda</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Muscles</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Italy</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Epidemiological investigations conducted during 10 trichinellosis outbreaks between 1975 and 1994 showed that horse-meat was the probable source of infection. Though hundreds of thousands of horses have been examined at abattoirs in America and Europe to detect Trichinella infection by artificial digestion or trichinelloscopy, an infected horse has never been detected during routine analysis, which consists of examining 1 g of tissue muscle from the diaphragm. In November 1996, a naturally infected horse imported from Romania was detected in Southern Italy. The parasite was identified as Trichinella spiralis by random amplified polymorphic DNA analysis. Artificial digestion of tissue samples from 60 different muscles from 13 different sites of the infected horse carcass showed that M. levator Labii maxillaris, M. hyoideus transversus, and M. buccinator were the 3 most infected muscles. Muscles from the tongue, the masseter, and the diaphragm, which have normally been considered the muscles of choice for diagnosis, were the 4th, 6th and 13th most infected muscles, respectively. When comparing body sites, muscle tissues from the head showed the highest level of infection, followed by muscles from the neck. This finding may explain the negative results that have been obtained in the past during routine examination of the diaphragm of horses.</abstract><qualityIndicators><score>5.17</score><pdfVersion>1.2</pdfVersion><pdfPageSize>461 x 684 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1360</abstractCharCount><pdfWordCount>2734</pdfWordCount><pdfCharCount>16574</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>203</abstractWordCount></qualityIndicators><title>Distribution of Trichinella spiralis larvae in muscles from a naturally infected horse</title><pii><json:string>S0304-4017(97)00141-6</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>74</volume><pii><json:string>S0304-4017(00)X0041-6</json:string></pii><pages><last>27</last><first>19</first></pages><issn><json:string>0304-4017</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Veterinary Parasitology</title><publicationDate>1998</publicationDate></host><categories><wos><json:string>science</json:string><json:string>veterinary sciences</json:string><json:string>parasitology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>mycology & parasitology</json:string></scienceMetrix></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1016/S0304-4017(97)00141-6</json:string></doi><id>E467CB5AC89DA0943ECA0C191902FA7A1389F032</id><score>0.15827611</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/E467CB5AC89DA0943ECA0C191902FA7A1389F032/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/E467CB5AC89DA0943ECA0C191902FA7A1389F032/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/E467CB5AC89DA0943ECA0C191902FA7A1389F032/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Distribution of Trichinella spiralis larvae in muscles from a naturally infected horse</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1998</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Distribution of Trichinella spiralis larvae in muscles from a naturally infected horse</title><author xml:id="author-1"><persName><forename type="first">E.</forename><surname>Pozio</surname></persName><email>pozio@pop3.iss.it</email><note type="correspondence"><p>Corresponding author. Tel.: +39-6-4990-2304; fax: +39-6-4938-7065</p></note><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation></author><author xml:id="author-2"><persName><forename type="first">G.V.</forename><surname>Celano</surname></persName><affiliation>Istituto di Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Bari, P.O. Box 7–70010 Valenzano, Bari, Italy</affiliation></author><author xml:id="author-3"><persName><forename type="first">L.</forename><surname>Sacchi</surname></persName><affiliation>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</affiliation></author><author xml:id="author-4"><persName><forename type="first">C.</forename><surname>Pavia</surname></persName><affiliation>Veterinary Service, Azienda Sanitaria Locale Bari 2, Barletta 70051, Italy</affiliation></author><author xml:id="author-5"><persName><forename type="first">P.</forename><surname>Rossi</surname></persName><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation></author><author xml:id="author-6"><persName><forename type="first">A.</forename><surname>Tamburrini</surname></persName><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation></author><author xml:id="author-7"><persName><forename type="first">S.</forename><surname>Corona</surname></persName><affiliation>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</affiliation></author><author xml:id="author-8"><persName><forename type="first">G.</forename><surname>La Rosa</surname></persName><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation></author></analytic><monogr><title level="j">Veterinary Parasitology</title><title level="j" type="abbrev">VETPAR</title><idno type="pISSN">0304-4017</idno><idno type="PII">S0304-4017(00)X0041-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">74</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="19">19</biblScope><biblScope unit="page" to="27">27</biblScope></imprint></monogr><idno type="istex">E467CB5AC89DA0943ECA0C191902FA7A1389F032</idno><idno type="DOI">10.1016/S0304-4017(97)00141-6</idno><idno type="PII">S0304-4017(97)00141-6</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Epidemiological investigations conducted during 10 trichinellosis outbreaks between 1975 and 1994 showed that horse-meat was the probable source of infection. Though hundreds of thousands of horses have been examined at abattoirs in America and Europe to detect Trichinella infection by artificial digestion or trichinelloscopy, an infected horse has never been detected during routine analysis, which consists of examining 1 g of tissue muscle from the diaphragm. In November 1996, a naturally infected horse imported from Romania was detected in Southern Italy. The parasite was identified as Trichinella spiralis by random amplified polymorphic DNA analysis. Artificial digestion of tissue samples from 60 different muscles from 13 different sites of the infected horse carcass showed that M. levator Labii maxillaris, M. hyoideus transversus, and M. buccinator were the 3 most infected muscles. Muscles from the tongue, the masseter, and the diaphragm, which have normally been considered the muscles of choice for diagnosis, were the 4th, 6th and 13th most infected muscles, respectively. When comparing body sites, muscle tissues from the head showed the highest level of infection, followed by muscles from the neck. This finding may explain the negative results that have been obtained in the past during routine examination of the diaphragm of horses.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Horse</term></item><item><term>Trichinella spiralis</term></item><item><term>Diagnosis nematoda</term></item><item><term>Muscles</term></item><item><term>Italy</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/E467CB5AC89DA0943ECA0C191902FA7A1389F032/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VETPAR</jid><aid>97001416</aid><ce:pii>S0304-4017(97)00141-6</ce:pii><ce:doi>10.1016/S0304-4017(97)00141-6</ce:doi><ce:copyright type="unknown" year="1998"></ce:copyright></item-info><head><ce:title>Distribution of <ce:italic>Trichinella spiralis</ce:italic> larvae in muscles from a naturally infected horse</ce:title><ce:author-group><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Pozio</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:e-address>pozio@pop3.iss.it</ce:e-address></ce:author><ce:author><ce:given-name>G.V.</ce:given-name><ce:surname>Celano</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>L.</ce:given-name><ce:surname>Sacchi</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.</ce:given-name><ce:surname>Pavia</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>P.</ce:given-name><ce:surname>Rossi</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Tamburrini</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Corona</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>G.</ce:given-name><ce:surname>La Rosa</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Istituto di Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Bari, P.O. Box 7–70010 Valenzano, Bari, Italy</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Veterinary Service, Azienda Sanitaria Locale Bari 2, Barletta 70051, Italy</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Corresponding author. Tel.: +39-6-4990-2304; fax: +39-6-4938-7065</ce:text></ce:correspondence></ce:author-group><ce:date-received day="14" month="4" year="1997"></ce:date-received><ce:date-accepted day="2" month="7" year="1997"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Epidemiological investigations conducted during 10 trichinellosis outbreaks between 1975 and 1994 showed that horse-meat was the probable source of infection. Though hundreds of thousands of horses have been examined at abattoirs in America and Europe to detect <ce:italic>Trichinella</ce:italic> infection by artificial digestion or trichinelloscopy, an infected horse has never been detected during routine analysis, which consists of examining 1 g of tissue muscle from the diaphragm. In November 1996, a naturally infected horse imported from Romania was detected in Southern Italy. The parasite was identified as <ce:italic>Trichinella spiralis</ce:italic> by random amplified polymorphic DNA analysis. Artificial digestion of tissue samples from 60 different muscles from 13 different sites of the infected horse carcass showed that <ce:italic>M. levator Labii maxillaris, M. hyoideus transversus</ce:italic>, and <ce:italic>M. buccinator</ce:italic> were the 3 most infected muscles. Muscles from the tongue, the masseter, and the diaphragm, which have normally been considered the muscles of choice for diagnosis, were the 4th, 6th and 13th most infected muscles, respectively. When comparing body sites, muscle tissues from the head showed the highest level of infection, followed by muscles from the neck. This finding may explain the negative results that have been obtained in the past during routine examination of the diaphragm of horses.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Horse</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Trichinella spiralis</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Diagnosis nematoda</ce:text></ce:keyword><ce:keyword><ce:text>Muscles</ce:text></ce:keyword><ce:keyword><ce:text>Italy</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Distribution of Trichinella spiralis larvae in muscles from a naturally infected horse</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Distribution of</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Pozio</namePart><affiliation>E-mail: pozio@pop3.iss.it</affiliation><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation><description>Corresponding author. Tel.: +39-6-4990-2304; fax: +39-6-4938-7065</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.V.</namePart><namePart type="family">Celano</namePart><affiliation>Istituto di Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Bari, P.O. Box 7–70010 Valenzano, Bari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Sacchi</namePart><affiliation>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Pavia</namePart><affiliation>Veterinary Service, Azienda Sanitaria Locale Bari 2, Barletta 70051, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Rossi</namePart><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Tamburrini</namePart><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Corona</namePart><affiliation>Department of Animal Biology, University of Pavia, Piazza Botta, 9–27100 Pavia, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">La Rosa</namePart><affiliation>Laboratory of Parasitology, Istituto Superiore di Sanità, viale Regina Elena, 299-00161 Rome, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Epidemiological investigations conducted during 10 trichinellosis outbreaks between 1975 and 1994 showed that horse-meat was the probable source of infection. Though hundreds of thousands of horses have been examined at abattoirs in America and Europe to detect Trichinella infection by artificial digestion or trichinelloscopy, an infected horse has never been detected during routine analysis, which consists of examining 1 g of tissue muscle from the diaphragm. In November 1996, a naturally infected horse imported from Romania was detected in Southern Italy. The parasite was identified as Trichinella spiralis by random amplified polymorphic DNA analysis. Artificial digestion of tissue samples from 60 different muscles from 13 different sites of the infected horse carcass showed that M. levator Labii maxillaris, M. hyoideus transversus, and M. buccinator were the 3 most infected muscles. Muscles from the tongue, the masseter, and the diaphragm, which have normally been considered the muscles of choice for diagnosis, were the 4th, 6th and 13th most infected muscles, respectively. When comparing body sites, muscle tissues from the head showed the highest level of infection, followed by muscles from the neck. This finding may explain the negative results that have been obtained in the past during routine examination of the diaphragm of horses.</abstract><subject><genre>Keywords</genre><topic>Horse</topic><topic>Trichinella spiralis</topic><topic>Diagnosis nematoda</topic><topic>Muscles</topic><topic>Italy</topic></subject><relatedItem type="host"><titleInfo><title>Veterinary Parasitology</title></titleInfo><titleInfo type="abbreviated"><title>VETPAR</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19980115</dateIssued></originInfo><identifier type="ISSN">0304-4017</identifier><identifier type="PII">S0304-4017(00)X0041-6</identifier><part><date>19980115</date><detail type="volume"><number>74</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>89</end></extent><extent unit="pages"><start>19</start><end>27</end></extent></part></relatedItem><identifier type="istex">E467CB5AC89DA0943ECA0C191902FA7A1389F032</identifier><identifier type="DOI">10.1016/S0304-4017(97)00141-6</identifier><identifier type="PII">S0304-4017(97)00141-6</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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