Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.
Identifieur interne : 001371 ( Main/Corpus ); précédent : 001370; suivant : 001372Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.
Auteurs : Megan R. France ; Diana L. Thomas ; Jia Liu ; Grant Mcfadden ; Amy L. Macneill ; Edward J. RoySource :
- The Journal of general virology [ 1465-2099 ] ; 2011.
English descriptors
- KwdEn :
- Animals (MeSH), Brain (pathology), Brain (virology), Ependyma (pathology), Ependyma (virology), Gene Expression (MeSH), Genes, Reporter (MeSH), Histocytochemistry (MeSH), Injections, Intraventricular (MeSH), Luminescent Proteins (genetics), Luminescent Proteins (metabolism), Mice (MeSH), Mice, Inbred C57BL (MeSH), Microscopy (MeSH), Myxoma virus (pathogenicity), Staining and Labeling (methods), Viral Proteins (biosynthesis).
- MESH :
- chemical , biosynthesis : Viral Proteins.
- chemical , genetics : Luminescent Proteins.
- chemical , metabolism : Luminescent Proteins.
- methods : Staining and Labeling.
- pathogenicity : Myxoma virus.
- pathology : Brain, Ependyma.
- virology : Brain, Ependyma.
- Animals, Gene Expression, Genes, Reporter, Histocytochemistry, Injections, Intraventricular, Mice, Mice, Inbred C57BL, Microscopy.
Abstract
Oncolytic viruses that selectively infect and lyse cancer cells have potential as therapeutic agents. Myxoma virus, a poxvirus that is known to be pathogenic only in rabbits, has not been reported to infect normal tissues in humans or mice. We observed that when recombinant virus was injected directly into the lateral ventricle of the mouse brain, virally encoded red fluorescent protein was expressed in ependymal and subventricular cells. Cells were positive for nestin, a marker of neural stem cells. Rapamycin increased the number of cells expressing the virally encoded protein. However, protein expression was transient. Cells expressing the virally encoded protein did not undergo apoptosis and the ependymal lining remained intact. Myxoma virus appears to be safe when injected into the brain despite the transient expression of virally derived protein in a small population of periventricular cells.
DOI: 10.1099/vir.0.026690-0
PubMed: 20861319
Links to Exploration step
pubmed:20861319Le document en format XML
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Thomas</name></author><author><name sortKey="Liu, Jia" sort="Liu, Jia" uniqKey="Liu J" first="Jia" last="Liu">Jia Liu</name></author><author><name sortKey="Mcfadden, Grant" sort="Mcfadden, Grant" uniqKey="Mcfadden G" first="Grant" last="Mcfadden">Grant Mcfadden</name></author><author><name sortKey="Macneill, Amy L" sort="Macneill, Amy L" uniqKey="Macneill A" first="Amy L" last="Macneill">Amy L. Macneill</name></author><author><name sortKey="Roy, Edward J" sort="Roy, Edward J" uniqKey="Roy E" first="Edward J" last="Roy">Edward J. 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Myxoma virus, a poxvirus that is known to be pathogenic only in rabbits, has not been reported to infect normal tissues in humans or mice. We observed that when recombinant virus was injected directly into the lateral ventricle of the mouse brain, virally encoded red fluorescent protein was expressed in ependymal and subventricular cells. Cells were positive for nestin, a marker of neural stem cells. Rapamycin increased the number of cells expressing the virally encoded protein. However, protein expression was transient. Cells expressing the virally encoded protein did not undergo apoptosis and the ependymal lining remained intact. Myxoma virus appears to be safe when injected into the brain despite the transient expression of virally derived protein in a small population of periventricular cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20861319</PMID><DateCompleted><Year>2011</Year><Month>01</Month><Day>18</Day></DateCompleted><DateRevised><Year>2010</Year><Month>12</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1465-2099</ISSN><JournalIssue CitedMedium="Internet"><Volume>92</Volume><Issue>Pt 1</Issue><PubDate><Year>2011</Year><Month>Jan</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J Gen Virol</ISOAbbreviation></Journal><ArticleTitle>Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.</ArticleTitle><Pagination><MedlinePgn>195-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.026690-0</ELocationID><Abstract><AbstractText>Oncolytic viruses that selectively infect and lyse cancer cells have potential as therapeutic agents. Myxoma virus, a poxvirus that is known to be pathogenic only in rabbits, has not been reported to infect normal tissues in humans or mice. We observed that when recombinant virus was injected directly into the lateral ventricle of the mouse brain, virally encoded red fluorescent protein was expressed in ependymal and subventricular cells. Cells were positive for nestin, a marker of neural stem cells. Rapamycin increased the number of cells expressing the virally encoded protein. However, protein expression was transient. Cells expressing the virally encoded protein did not undergo apoptosis and the ependymal lining remained intact. Myxoma virus appears to be safe when injected into the brain despite the transient expression of virally derived protein in a small population of periventricular cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>France</LastName><ForeName>Megan R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Department of Molecular and Integrative Physiology, University of Illinois, Urbana-Champaign, IL 61801, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>Diana L</ForeName><Initials>DL</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jia</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>McFadden</LastName><ForeName>Grant</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>MacNeill</LastName><ForeName>Amy L</ForeName><Initials>AL</Initials></Author><Author ValidYN="Y"><LastName>Roy</LastName><ForeName>Edward J</ForeName><Initials>EJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>09</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C413662">red fluorescent protein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001921" MajorTopicYN="N">Brain</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004805" MajorTopicYN="N">Ependyma</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006651" MajorTopicYN="N">Histocytochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007276" MajorTopicYN="N">Injections, Intraventricular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008853" MajorTopicYN="N">Microscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009233" MajorTopicYN="N">Myxoma virus</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20861319</ArticleId><ArticleId IdType="pii">vir.0.026690-0</ArticleId><ArticleId IdType="doi">10.1099/vir.0.026690-0</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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