Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.
Identifieur interne : 001371 ( Main/Corpus ); précédent : 001370; suivant : 001372Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.
Auteurs : Megan R. France ; Diana L. Thomas ; Jia Liu ; Grant Mcfadden ; Amy L. Macneill ; Edward J. RoySource :
- The Journal of general virology [ 1465-2099 ] ; 2011.
English descriptors
- KwdEn :
- Animals (MeSH), Brain (pathology), Brain (virology), Ependyma (pathology), Ependyma (virology), Gene Expression (MeSH), Genes, Reporter (MeSH), Histocytochemistry (MeSH), Injections, Intraventricular (MeSH), Luminescent Proteins (genetics), Luminescent Proteins (metabolism), Mice (MeSH), Mice, Inbred C57BL (MeSH), Microscopy (MeSH), Myxoma virus (pathogenicity), Staining and Labeling (methods), Viral Proteins (biosynthesis).
- MESH :
- chemical , biosynthesis : Viral Proteins.
- chemical , genetics : Luminescent Proteins.
- chemical , metabolism : Luminescent Proteins.
- methods : Staining and Labeling.
- pathogenicity : Myxoma virus.
- pathology : Brain, Ependyma.
- virology : Brain, Ependyma.
- Animals, Gene Expression, Genes, Reporter, Histocytochemistry, Injections, Intraventricular, Mice, Mice, Inbred C57BL, Microscopy.
Abstract
Oncolytic viruses that selectively infect and lyse cancer cells have potential as therapeutic agents. Myxoma virus, a poxvirus that is known to be pathogenic only in rabbits, has not been reported to infect normal tissues in humans or mice. We observed that when recombinant virus was injected directly into the lateral ventricle of the mouse brain, virally encoded red fluorescent protein was expressed in ependymal and subventricular cells. Cells were positive for nestin, a marker of neural stem cells. Rapamycin increased the number of cells expressing the virally encoded protein. However, protein expression was transient. Cells expressing the virally encoded protein did not undergo apoptosis and the ependymal lining remained intact. Myxoma virus appears to be safe when injected into the brain despite the transient expression of virally derived protein in a small population of periventricular cells.
DOI: 10.1099/vir.0.026690-0
PubMed: 20861319
Links to Exploration step
pubmed:20861319Le document en format XML
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<author><name sortKey="France, Megan R" sort="France, Megan R" uniqKey="France M" first="Megan R" last="France">Megan R. France</name>
<affiliation><nlm:affiliation>Department of Molecular and Integrative Physiology, University of Illinois, Urbana-Champaign, IL 61801, USA.</nlm:affiliation>
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<author><name sortKey="Thomas, Diana L" sort="Thomas, Diana L" uniqKey="Thomas D" first="Diana L" last="Thomas">Diana L. Thomas</name>
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<author><name sortKey="Liu, Jia" sort="Liu, Jia" uniqKey="Liu J" first="Jia" last="Liu">Jia Liu</name>
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<author><name sortKey="Mcfadden, Grant" sort="Mcfadden, Grant" uniqKey="Mcfadden G" first="Grant" last="Mcfadden">Grant Mcfadden</name>
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<author><name sortKey="Macneill, Amy L" sort="Macneill, Amy L" uniqKey="Macneill A" first="Amy L" last="Macneill">Amy L. Macneill</name>
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<author><name sortKey="Roy, Edward J" sort="Roy, Edward J" uniqKey="Roy E" first="Edward J" last="Roy">Edward J. Roy</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.</title>
<author><name sortKey="France, Megan R" sort="France, Megan R" uniqKey="France M" first="Megan R" last="France">Megan R. France</name>
<affiliation><nlm:affiliation>Department of Molecular and Integrative Physiology, University of Illinois, Urbana-Champaign, IL 61801, USA.</nlm:affiliation>
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<author><name sortKey="Thomas, Diana L" sort="Thomas, Diana L" uniqKey="Thomas D" first="Diana L" last="Thomas">Diana L. Thomas</name>
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<author><name sortKey="Liu, Jia" sort="Liu, Jia" uniqKey="Liu J" first="Jia" last="Liu">Jia Liu</name>
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<author><name sortKey="Mcfadden, Grant" sort="Mcfadden, Grant" uniqKey="Mcfadden G" first="Grant" last="Mcfadden">Grant Mcfadden</name>
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<author><name sortKey="Macneill, Amy L" sort="Macneill, Amy L" uniqKey="Macneill A" first="Amy L" last="Macneill">Amy L. Macneill</name>
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<author><name sortKey="Roy, Edward J" sort="Roy, Edward J" uniqKey="Roy E" first="Edward J" last="Roy">Edward J. Roy</name>
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<series><title level="j">The Journal of general virology</title>
<idno type="eISSN">1465-2099</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Brain (pathology)</term>
<term>Brain (virology)</term>
<term>Ependyma (pathology)</term>
<term>Ependyma (virology)</term>
<term>Gene Expression (MeSH)</term>
<term>Genes, Reporter (MeSH)</term>
<term>Histocytochemistry (MeSH)</term>
<term>Injections, Intraventricular (MeSH)</term>
<term>Luminescent Proteins (genetics)</term>
<term>Luminescent Proteins (metabolism)</term>
<term>Mice (MeSH)</term>
<term>Mice, Inbred C57BL (MeSH)</term>
<term>Microscopy (MeSH)</term>
<term>Myxoma virus (pathogenicity)</term>
<term>Staining and Labeling (methods)</term>
<term>Viral Proteins (biosynthesis)</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Viral Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luminescent Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Luminescent Proteins</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Staining and Labeling</term>
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<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Myxoma virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Brain</term>
<term>Ependyma</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Brain</term>
<term>Ependyma</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Gene Expression</term>
<term>Genes, Reporter</term>
<term>Histocytochemistry</term>
<term>Injections, Intraventricular</term>
<term>Mice</term>
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<front><div type="abstract" xml:lang="en">Oncolytic viruses that selectively infect and lyse cancer cells have potential as therapeutic agents. Myxoma virus, a poxvirus that is known to be pathogenic only in rabbits, has not been reported to infect normal tissues in humans or mice. We observed that when recombinant virus was injected directly into the lateral ventricle of the mouse brain, virally encoded red fluorescent protein was expressed in ependymal and subventricular cells. Cells were positive for nestin, a marker of neural stem cells. Rapamycin increased the number of cells expressing the virally encoded protein. However, protein expression was transient. Cells expressing the virally encoded protein did not undergo apoptosis and the ependymal lining remained intact. Myxoma virus appears to be safe when injected into the brain despite the transient expression of virally derived protein in a small population of periventricular cells.</div>
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<ArticleTitle>Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells.</ArticleTitle>
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<Abstract><AbstractText>Oncolytic viruses that selectively infect and lyse cancer cells have potential as therapeutic agents. Myxoma virus, a poxvirus that is known to be pathogenic only in rabbits, has not been reported to infect normal tissues in humans or mice. We observed that when recombinant virus was injected directly into the lateral ventricle of the mouse brain, virally encoded red fluorescent protein was expressed in ependymal and subventricular cells. Cells were positive for nestin, a marker of neural stem cells. Rapamycin increased the number of cells expressing the virally encoded protein. However, protein expression was transient. Cells expressing the virally encoded protein did not undergo apoptosis and the ependymal lining remained intact. Myxoma virus appears to be safe when injected into the brain despite the transient expression of virally derived protein in a small population of periventricular cells.</AbstractText>
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