Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.
Identifieur interne : 000905 ( Main/Corpus ); précédent : 000904; suivant : 000906Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.
Auteurs : Zhenkai Dai ; Jianfei Huang ; Xiaoya Lei ; Yiming Yan ; Piaopiao Lu ; Huanmin Zhang ; Wencheng Lin ; Weiguo Chen ; Jingyun Ma ; Qingmei XieSource :
- Vaccine [ 1873-2518 ] ; 2017.
English descriptors
- KwdEn :
- Adaptive Immunity (drug effects), Animals (MeSH), Antibodies, Viral (biosynthesis), Autophagy (drug effects), Autophagy (genetics), Autophagy (immunology), Avian Leukosis (genetics), Avian Leukosis (immunology), Avian Leukosis (prevention & control), Avian Leukosis (virology), Avian Leukosis Virus (drug effects), Avian Leukosis Virus (immunology), Chickens (MeSH), Electroporation (MeSH), Genetic Vectors (chemistry), Genetic Vectors (immunology), Immunity, Innate (drug effects), Interferon-gamma (genetics), Interferon-gamma (immunology), Interleukin-10 (genetics), Interleukin-10 (immunology), Interleukin-2 (genetics), Interleukin-2 (immunology), Poultry Diseases (genetics), Poultry Diseases (immunology), Poultry Diseases (prevention & control), Poultry Diseases (virology), Sirolimus (pharmacology), T-Lymphocytes (drug effects), T-Lymphocytes (immunology), T-Lymphocytes (virology), Vaccination (MeSH), Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, DNA, Viral Vaccines.
- chemical , biosynthesis : Antibodies, Viral.
- chemistry : Genetic Vectors.
- drug effects : Adaptive Immunity, Autophagy, Avian Leukosis Virus, Immunity, Innate, T-Lymphocytes.
- genetics : Autophagy, Avian Leukosis, Interferon-gamma, Interleukin-10, Interleukin-2, Poultry Diseases, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- immunology : Autophagy, Avian Leukosis, Avian Leukosis Virus, Genetic Vectors, Interferon-gamma, Interleukin-10, Interleukin-2, Poultry Diseases, T-Lymphocytes, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- chemical , pharmacology : Sirolimus.
- prevention & control : Avian Leukosis, Poultry Diseases.
- virology : Avian Leukosis, Poultry Diseases, T-Lymphocytes.
- Animals, Chickens, Electroporation, Vaccination.
Abstract
Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-γ expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+CD8+T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J.
DOI: 10.1016/j.vaccine.2016.12.034
PubMed: 28049588
Links to Exploration step
pubmed:28049588Le document en format XML
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<author><name sortKey="Chen, Weiguo" sort="Chen, Weiguo" uniqKey="Chen W" first="Weiguo" last="Chen">Weiguo Chen</name>
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<author><name sortKey="Ma, Jingyun" sort="Ma, Jingyun" uniqKey="Ma J" first="Jingyun" last="Ma">Jingyun Ma</name>
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<author><name sortKey="Xie, Qingmei" sort="Xie, Qingmei" uniqKey="Xie Q" first="Qingmei" last="Xie">Qingmei Xie</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China. Electronic address: qmx@scau.edu.cn.</nlm:affiliation>
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<author><name sortKey="Dai, Zhenkai" sort="Dai, Zhenkai" uniqKey="Dai Z" first="Zhenkai" last="Dai">Zhenkai Dai</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
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<author><name sortKey="Huang, Jianfei" sort="Huang, Jianfei" uniqKey="Huang J" first="Jianfei" last="Huang">Jianfei Huang</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
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<author><name sortKey="Lei, Xiaoya" sort="Lei, Xiaoya" uniqKey="Lei X" first="Xiaoya" last="Lei">Xiaoya Lei</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
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<author><name sortKey="Yan, Yiming" sort="Yan, Yiming" uniqKey="Yan Y" first="Yiming" last="Yan">Yiming Yan</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
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<author><name sortKey="Lu, Piaopiao" sort="Lu, Piaopiao" uniqKey="Lu P" first="Piaopiao" last="Lu">Piaopiao Lu</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
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<author><name sortKey="Zhang, Huanmin" sort="Zhang, Huanmin" uniqKey="Zhang H" first="Huanmin" last="Zhang">Huanmin Zhang</name>
<affiliation><nlm:affiliation>USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Lin, Wencheng" sort="Lin, Wencheng" uniqKey="Lin W" first="Wencheng" last="Lin">Wencheng Lin</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</nlm:affiliation>
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<author><name sortKey="Chen, Weiguo" sort="Chen, Weiguo" uniqKey="Chen W" first="Weiguo" last="Chen">Weiguo Chen</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Ma, Jingyun" sort="Ma, Jingyun" uniqKey="Ma J" first="Jingyun" last="Ma">Jingyun Ma</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Xie, Qingmei" sort="Xie, Qingmei" uniqKey="Xie Q" first="Qingmei" last="Xie">Qingmei Xie</name>
<affiliation><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China. Electronic address: qmx@scau.edu.cn.</nlm:affiliation>
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<series><title level="j">Vaccine</title>
<idno type="eISSN">1873-2518</idno>
<imprint><date when="2017" type="published">2017</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptive Immunity (drug effects)</term>
<term>Animals (MeSH)</term>
<term>Antibodies, Viral (biosynthesis)</term>
<term>Autophagy (drug effects)</term>
<term>Autophagy (genetics)</term>
<term>Autophagy (immunology)</term>
<term>Avian Leukosis (genetics)</term>
<term>Avian Leukosis (immunology)</term>
<term>Avian Leukosis (prevention & control)</term>
<term>Avian Leukosis (virology)</term>
<term>Avian Leukosis Virus (drug effects)</term>
<term>Avian Leukosis Virus (immunology)</term>
<term>Chickens (MeSH)</term>
<term>Electroporation (MeSH)</term>
<term>Genetic Vectors (chemistry)</term>
<term>Genetic Vectors (immunology)</term>
<term>Immunity, Innate (drug effects)</term>
<term>Interferon-gamma (genetics)</term>
<term>Interferon-gamma (immunology)</term>
<term>Interleukin-10 (genetics)</term>
<term>Interleukin-10 (immunology)</term>
<term>Interleukin-2 (genetics)</term>
<term>Interleukin-2 (immunology)</term>
<term>Poultry Diseases (genetics)</term>
<term>Poultry Diseases (immunology)</term>
<term>Poultry Diseases (prevention & control)</term>
<term>Poultry Diseases (virology)</term>
<term>Sirolimus (pharmacology)</term>
<term>T-Lymphocytes (drug effects)</term>
<term>T-Lymphocytes (immunology)</term>
<term>T-Lymphocytes (virology)</term>
<term>Vaccination (MeSH)</term>
<term>Vaccines, DNA (administration & dosage)</term>
<term>Vaccines, DNA (genetics)</term>
<term>Vaccines, DNA (immunology)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Viral Vaccines (genetics)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, DNA</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Genetic Vectors</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Adaptive Immunity</term>
<term>Autophagy</term>
<term>Avian Leukosis Virus</term>
<term>Immunity, Innate</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Autophagy</term>
<term>Avian Leukosis</term>
<term>Interferon-gamma</term>
<term>Interleukin-10</term>
<term>Interleukin-2</term>
<term>Poultry Diseases</term>
<term>Vaccines, DNA</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Autophagy</term>
<term>Avian Leukosis</term>
<term>Avian Leukosis Virus</term>
<term>Genetic Vectors</term>
<term>Interferon-gamma</term>
<term>Interleukin-10</term>
<term>Interleukin-2</term>
<term>Poultry Diseases</term>
<term>T-Lymphocytes</term>
<term>Vaccines, DNA</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sirolimus</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Avian Leukosis</term>
<term>Poultry Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Avian Leukosis</term>
<term>Poultry Diseases</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chickens</term>
<term>Electroporation</term>
<term>Vaccination</term>
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<front><div type="abstract" xml:lang="en">Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-γ expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+CD8+T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J.</div>
</front>
</TEI>
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<DateCompleted><Year>2017</Year>
<Month>12</Month>
<Day>08</Day>
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<DateRevised><Year>2017</Year>
<Month>12</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2518</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>35</Volume>
<Issue>5</Issue>
<PubDate><Year>2017</Year>
<Month>02</Month>
<Day>01</Day>
</PubDate>
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<Title>Vaccine</Title>
<ISOAbbreviation>Vaccine</ISOAbbreviation>
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<ArticleTitle>Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.</ArticleTitle>
<Pagination><MedlinePgn>808-813</MedlinePgn>
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<Abstract><AbstractText>Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-γ expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+CD8+T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J.</AbstractText>
<CopyrightInformation>Copyright © 2016 Elsevier Ltd. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dai</LastName>
<ForeName>Zhenkai</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Jianfei</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lei</LastName>
<ForeName>Xiaoya</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Yiming</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lu</LastName>
<ForeName>Piaopiao</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Huanmin</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Wencheng</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Weiguo</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ma</LastName>
<ForeName>Jingyun</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xie</LastName>
<ForeName>Qingmei</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China. Electronic address: qmx@scau.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>12</Month>
<Day>31</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Vaccine</MedlineTA>
<NlmUniqueID>8406899</NlmUniqueID>
<ISSNLinking>0264-410X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007376">Interleukin-2</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>130068-27-8</RegistryNumber>
<NameOfSubstance UI="D016753">Interleukin-10</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>82115-62-6</RegistryNumber>
<NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>W36ZG6FT64</RegistryNumber>
<NameOfSubstance UI="D020123">Sirolimus</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D056704" MajorTopicYN="N">Adaptive Immunity</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001343" MajorTopicYN="N">Autophagy</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001353" MajorTopicYN="N">Avian Leukosis</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001354" MajorTopicYN="N">Avian Leukosis Virus</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018274" MajorTopicYN="N">Electroporation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016753" MajorTopicYN="N">Interleukin-10</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007376" MajorTopicYN="N">Interleukin-2</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011201" MajorTopicYN="N">Poultry Diseases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014611" MajorTopicYN="Y">Vaccination</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Autophagy</Keyword>
<Keyword MajorTopicYN="Y">Avian leucosis virus</Keyword>
<Keyword MajorTopicYN="Y">DNA vaccine</Keyword>
<Keyword MajorTopicYN="Y">Rapamycin</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>07</Month>
<Day>24</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>12</Month>
<Day>12</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>12</Month>
<Day>14</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>12</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28049588</ArticleId>
<ArticleId IdType="pii">S0264-410X(16)31259-2</ArticleId>
<ArticleId IdType="doi">10.1016/j.vaccine.2016.12.034</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
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