Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.
Identifieur interne : 000667 ( Main/Corpus ); précédent : 000666; suivant : 000668Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.
Auteurs : Yuanxing Gu ; Yingshan Zhou ; Xinfeng Shi ; Yongping Xin ; Ying Shan ; Cong Chen ; Tong Cao ; Weihuan Fang ; Xiaoliang LiSource :
- Archives of virology [ 1432-8798 ] ; 2018.
English descriptors
- KwdEn :
- Adenine (analogs & derivatives), Adenine (pharmacology), Androstadienes (pharmacology), Animals (MeSH), Autophagy (drug effects), Autophagy (genetics), Autophagy-Related Protein 5 (antagonists & inhibitors), Autophagy-Related Protein 5 (genetics), Autophagy-Related Protein 5 (metabolism), Cell Line (MeSH), Epithelial Cells (drug effects), Epithelial Cells (metabolism), Epithelial Cells (virology), Gene Expression Regulation (MeSH), Genes, Reporter (MeSH), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Host-Pathogen Interactions (MeSH), Kidney (MeSH), Microtubule-Associated Proteins (genetics), Microtubule-Associated Proteins (metabolism), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), Signal Transduction (MeSH), Sirolimus (pharmacology), Swine (MeSH), Teschovirus (drug effects), Teschovirus (genetics), Teschovirus (growth & development), Teschovirus (metabolism), Virus Replication (drug effects), Virus Replication (genetics), Wortmannin (MeSH).
- MESH :
- chemical , analogs & derivatives : Adenine.
- chemical , antagonists & inhibitors : Autophagy-Related Protein 5.
- chemical , genetics : Autophagy-Related Protein 5, Green Fluorescent Proteins, Microtubule-Associated Proteins, RNA, Small Interfering.
- chemical , metabolism : Autophagy-Related Protein 5, Green Fluorescent Proteins, Microtubule-Associated Proteins, RNA, Small Interfering.
- chemical , pharmacology : Adenine, Androstadienes, Sirolimus.
- drug effects : Autophagy, Epithelial Cells, Teschovirus, Virus Replication.
- genetics : Autophagy, Teschovirus, Virus Replication.
- growth & development : Teschovirus.
- metabolism : Epithelial Cells, Teschovirus.
- virology : Epithelial Cells.
- Animals, Cell Line, Gene Expression Regulation, Genes, Reporter, Host-Pathogen Interactions, Kidney, Signal Transduction, Swine, Wortmannin.
Abstract
Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.
DOI: 10.1007/s00705-017-3652-2
PubMed: 29177545
Links to Exploration step
pubmed:29177545Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.</title><author><name sortKey="Gu, Yuanxing" sort="Gu, Yuanxing" uniqKey="Gu Y" first="Yuanxing" last="Gu">Yuanxing Gu</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Qingdao Agricultural University, Qingdao, 266109, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Yingshan" sort="Zhou, Yingshan" uniqKey="Zhou Y" first="Yingshan" last="Zhou">Yingshan Zhou</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Animal Science and Technology, China-Australia Joint-Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Zhejiang A&F University, Lin'an, 311300, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Xinfeng" sort="Shi, Xinfeng" uniqKey="Shi X" first="Xinfeng" last="Shi">Xinfeng Shi</name><affiliation><nlm:affiliation>Animal Products Quality Testing Center of Zhejiang Province, Hangzhou, 310020, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xin, Yongping" sort="Xin, Yongping" uniqKey="Xin Y" first="Yongping" last="Xin">Yongping Xin</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shan, Ying" sort="Shan, Ying" uniqKey="Shan Y" first="Ying" last="Shan">Ying Shan</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Cong" sort="Chen, Cong" uniqKey="Chen C" first="Cong" last="Chen">Cong Chen</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Tong" sort="Cao, Tong" uniqKey="Cao T" first="Tong" last="Cao">Tong Cao</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Fang, Weihuan" sort="Fang, Weihuan" uniqKey="Fang W" first="Weihuan" last="Fang">Weihuan Fang</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Xiaoliang" sort="Li, Xiaoliang" uniqKey="Li X" first="Xiaoliang" last="Li">Xiaoliang Li</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. xlli@zju.edu.cn.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29177545</idno><idno type="pmid">29177545</idno><idno type="doi">10.1007/s00705-017-3652-2</idno><idno type="wicri:Area/Main/Corpus">000667</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000667</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.</title><author><name sortKey="Gu, Yuanxing" sort="Gu, Yuanxing" uniqKey="Gu Y" first="Yuanxing" last="Gu">Yuanxing Gu</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Qingdao Agricultural University, Qingdao, 266109, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Yingshan" sort="Zhou, Yingshan" uniqKey="Zhou Y" first="Yingshan" last="Zhou">Yingshan Zhou</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Animal Science and Technology, China-Australia Joint-Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Zhejiang A&F University, Lin'an, 311300, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Xinfeng" sort="Shi, Xinfeng" uniqKey="Shi X" first="Xinfeng" last="Shi">Xinfeng Shi</name><affiliation><nlm:affiliation>Animal Products Quality Testing Center of Zhejiang Province, Hangzhou, 310020, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xin, Yongping" sort="Xin, Yongping" uniqKey="Xin Y" first="Yongping" last="Xin">Yongping Xin</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shan, Ying" sort="Shan, Ying" uniqKey="Shan Y" first="Ying" last="Shan">Ying Shan</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Cong" sort="Chen, Cong" uniqKey="Chen C" first="Cong" last="Chen">Cong Chen</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Tong" sort="Cao, Tong" uniqKey="Cao T" first="Tong" last="Cao">Tong Cao</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Fang, Weihuan" sort="Fang, Weihuan" uniqKey="Fang W" first="Weihuan" last="Fang">Weihuan Fang</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Xiaoliang" sort="Li, Xiaoliang" uniqKey="Li X" first="Xiaoliang" last="Li">Xiaoliang Li</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. xlli@zju.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Archives of virology</title><idno type="eISSN">1432-8798</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenine (analogs & derivatives)</term><term>Adenine (pharmacology)</term><term>Androstadienes (pharmacology)</term><term>Animals (MeSH)</term><term>Autophagy (drug effects)</term><term>Autophagy (genetics)</term><term>Autophagy-Related Protein 5 (antagonists & inhibitors)</term><term>Autophagy-Related Protein 5 (genetics)</term><term>Autophagy-Related Protein 5 (metabolism)</term><term>Cell Line (MeSH)</term><term>Epithelial Cells (drug effects)</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Gene Expression Regulation (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Kidney (MeSH)</term><term>Microtubule-Associated Proteins (genetics)</term><term>Microtubule-Associated Proteins (metabolism)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>Signal Transduction (MeSH)</term><term>Sirolimus (pharmacology)</term><term>Swine (MeSH)</term><term>Teschovirus (drug effects)</term><term>Teschovirus (genetics)</term><term>Teschovirus (growth & development)</term><term>Teschovirus (metabolism)</term><term>Virus Replication (drug effects)</term><term>Virus Replication (genetics)</term><term>Wortmannin (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Adenine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Autophagy-Related Protein 5</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Autophagy-Related Protein 5</term><term>Green Fluorescent Proteins</term><term>Microtubule-Associated Proteins</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Autophagy-Related Protein 5</term><term>Green Fluorescent Proteins</term><term>Microtubule-Associated Proteins</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adenine</term><term>Androstadienes</term><term>Sirolimus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Autophagy</term><term>Epithelial Cells</term><term>Teschovirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Autophagy</term><term>Teschovirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Teschovirus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>Teschovirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Gene Expression Regulation</term><term>Genes, Reporter</term><term>Host-Pathogen Interactions</term><term>Kidney</term><term>Signal Transduction</term><term>Swine</term><term>Wortmannin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29177545</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>20</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-8798</ISSN><JournalIssue CitedMedium="Internet"><Volume>163</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Archives of virology</Title><ISOAbbreviation>Arch Virol</ISOAbbreviation></Journal><ArticleTitle>Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.</ArticleTitle><Pagination><MedlinePgn>623-632</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00705-017-3652-2</ELocationID><Abstract><AbstractText>Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Yuanxing</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Qingdao Agricultural University, Qingdao, 266109, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yingshan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Animal Science and Technology, China-Australia Joint-Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Zhejiang A&F University, Lin'an, 311300, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Xinfeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Animal Products Quality Testing Center of Zhejiang Province, Hangzhou, 310020, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xin</LastName><ForeName>Yongping</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shan</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Cong</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Tong</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Weihuan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiaoliang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. xlli@zju.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2015C02044</GrantID><Agency>Department of S&T of Zhejiang Province</Agency><Country></Country></Grant><Grant><GrantID>Y201635576</GrantID><Agency>Department of Education of Zhejiang Province</Agency><Country></Country></Grant><Grant><GrantID>2017ZDNT004</GrantID><Agency>Dabei Agricultural Discipline Development and Talent Training Fund</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>11</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Arch Virol</MedlineTA><NlmUniqueID>7506870</NlmUniqueID><ISSNLinking>0304-8608</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000730">Androstadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000071187">Autophagy-Related Protein 5</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008869">Microtubule-Associated Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>5142-23-4</RegistryNumber><NameOfSubstance UI="C025946">3-methyladenine</NameOfSubstance></Chemical><Chemical><RegistryNumber>JAC85A2161</RegistryNumber><NameOfSubstance UI="D000225">Adenine</NameOfSubstance></Chemical><Chemical><RegistryNumber>W36ZG6FT64</RegistryNumber><NameOfSubstance UI="D020123">Sirolimus</NameOfSubstance></Chemical><Chemical><RegistryNumber>XVA4O219QW</RegistryNumber><NameOfSubstance UI="D000077191">Wortmannin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000225" MajorTopicYN="N">Adenine</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000730" MajorTopicYN="N">Androstadienes</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="N">Autophagy</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000071187" MajorTopicYN="N">Autophagy-Related Protein 5</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="Y">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007668" MajorTopicYN="N">Kidney</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008869" MajorTopicYN="N">Microtubule-Associated Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034741" MajorTopicYN="N">RNA, Small Interfering</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052700" MajorTopicYN="N">Teschovirus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000077191" MajorTopicYN="N">Wortmannin</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>06</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>10</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29177545</ArticleId><ArticleId IdType="doi">10.1007/s00705-017-3652-2</ArticleId><ArticleId IdType="pii">10.1007/s00705-017-3652-2</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000667 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000667 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:29177545
|texte= Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:29177545" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |