Loss of heterozygosity by SCRaMbLEing.
Identifieur interne : 000316 ( Main/Corpus ); précédent : 000315; suivant : 000317Loss of heterozygosity by SCRaMbLEing.
Auteurs : Yunxiang Li ; Yi Wu ; Lu Ma ; Zhou Guo ; Wenhai Xiao ; Yingjin YuanSource :
- Science China. Life sciences [ 1869-1889 ] ; 2019.
English descriptors
- KwdEn :
- Aneuploidy (MeSH), Chromosomes, Fungal (genetics), Drug Resistance, Microbial (drug effects), Drug Resistance, Microbial (genetics), Evolution, Molecular (MeSH), Genetic Techniques (MeSH), Genetic Variation (MeSH), Genome, Fungal (genetics), Genomic Instability (MeSH), Heterozygote (MeSH), Humans (MeSH), Loss of Heterozygosity (genetics), Phenotype (MeSH), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae Proteins (genetics), Sirolimus (pharmacology), Transcription Factors (genetics).
- MESH :
- chemical , genetics : Saccharomyces cerevisiae Proteins, Transcription Factors.
- drug effects : Drug Resistance, Microbial.
- genetics : Chromosomes, Fungal, Drug Resistance, Microbial, Genome, Fungal, Loss of Heterozygosity, Saccharomyces cerevisiae.
- chemical , pharmacology : Sirolimus.
- Aneuploidy, Evolution, Molecular, Genetic Techniques, Genetic Variation, Genomic Instability, Heterozygote, Humans, Phenotype.
Abstract
Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE, different levels of loss of heterozygosity (LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome X, whole chromosome LOH of synthetic chromosome X, and duplication of chromosome VIII (trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function.
DOI: 10.1007/s11427-019-9504-5
PubMed: 30900161
Links to Exploration step
pubmed:30900161Le document en format XML
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<term>Drug Resistance, Microbial (genetics)</term>
<term>Evolution, Molecular (MeSH)</term>
<term>Genetic Techniques (MeSH)</term>
<term>Genetic Variation (MeSH)</term>
<term>Genome, Fungal (genetics)</term>
<term>Genomic Instability (MeSH)</term>
<term>Heterozygote (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Loss of Heterozygosity (genetics)</term>
<term>Phenotype (MeSH)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae Proteins (genetics)</term>
<term>Sirolimus (pharmacology)</term>
<term>Transcription Factors (genetics)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Drug Resistance, Microbial</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Fungal</term>
<term>Drug Resistance, Microbial</term>
<term>Genome, Fungal</term>
<term>Loss of Heterozygosity</term>
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<term>Genetic Techniques</term>
<term>Genetic Variation</term>
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<front><div type="abstract" xml:lang="en">Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE, different levels of loss of heterozygosity (LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome X, whole chromosome LOH of synthetic chromosome X, and duplication of chromosome VIII (trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function.</div>
</front>
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<Abstract><AbstractText>Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE, different levels of loss of heterozygosity (LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome X, whole chromosome LOH of synthetic chromosome X, and duplication of chromosome VIII (trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function.</AbstractText>
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<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019656" MajorTopicYN="N">Loss of Heterozygosity</DescriptorName>
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<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName>
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<Keyword MajorTopicYN="N">aneuploidy</Keyword>
<Keyword MajorTopicYN="N">loss of heterozygosity (LOH)</Keyword>
<Keyword MajorTopicYN="N">rapid adaptive evolution</Keyword>
<Keyword MajorTopicYN="N">structural variation</Keyword>
<Keyword MajorTopicYN="N">synthetic yeast genome</Keyword>
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<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
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